IL24 (BC009681) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	C49A; FISP; IL-24; IL-24 splice variant delE3; IL-24 splice variant delE5; IL10B; Interleukin 24; mda-7; MDA7; melanoma differentiation association protein 7; Mob-5; OTTHUMP00000034620; ST16; suppression of tumorigenicity 16 (melanoma differentiation)
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC009681 edited ATGACTTCCACGGCTGGGACGGGAACCTTCCACCCACAGCTATGCCTCTGATTGGTGAAT GGTGAAGGTGCCTGTCTAACTTTTCTGTAAAAAGAACCAGCTGCCTCCAGGCAGCCAGCC CTCAAGCATCACTTACAGGACCAGAGGGACAAGACATGACTGTGATGAGGAGCTGCTTTC GCCAATTTAACACCAAGAAGAATTGAGGCTGCTTGGGAGGAAGGCCAGGAGGAACACGAG ACTGAGAGATGAATTTTCAACAGAGGCTGCAAAGCCTGTGGACTTTAGCCAGCAGACCCT TCTGCCCTCCTTTGCTGGCGACAGCCTCTCAAATGCAGATGGTTGTGCTCCCTTGCCTGG GTTTTACCCTGCTTCTCTGGAGCCAGGTATCAGGGGCCCAGGGCCAAGAATTCCACTTTG GGCCCTGCCAAGTGAAGGGGGTTGTTCCCCAGAAACTGTGGGAAGCCTTCTGGGCTGTGA AAGACACTATGCAAGCTCAGGATAACATCACGAGTGCCCGGCTGCTGCAGCAGGAGGTTC TGCAGAACGTCTCGGATGCTGAGAGCTGTTACCTTGTCCACACCCTGCTGGAGTTCTACT TGAAAACTGTTTTCAAAAACTACCACAATAGAACAGTTGAAGTCAGGACTCTGAAGTCAT TCTCTACTCTGGCCAACAACTTTGTTCTCATCGTGTCACAACTGCAACCCAGTCAAGAAA ATGAGATGTTTTCCATCAGAGACAGTGCACACAGGCGGTTTCTGCTATTCCGGAGAGCAT TCAAACAGTTGGACGTAGAAGCAGCTCTGACCAAAGCCCTTGGGGAAGTGGACATTCTTC TGACCTGGATGCAGAAATTCTACAAGCTCTGAATGTCTAGACCAGGACCTCCCTCCCCCT GGCACTGGTTTGTTCCCTGTGTCATTTCAAACAGTCTCCCTTCCTATGCTGTTCACTGGA CACTTCACGCCCTTGGCCATGGGTCCCATTCTTGGCCCAGGATTATTGTCAAAGAAGTCA TTCTTTAAGCAGCGCCAGTGACAGTCAGGGAAGGTGCCTCTGGATGCTGTGAAGAGTCTA CAGAGAAGATTCTTGTATTTATTACAACTCTATTTAATTAATGTCAGTATTTCAACTGAA GTTCTATTTATTTGTGAGACTGTAAGTTACATGAAGGCAGCAGAATATTGTGCCCCATGC TTCTTTACCCCTCACAATCCTTGCCACAGTGTGGGGCAGTGGATGGGTGCTTAGTAAGTA CTTAATAAACTGTGGTGCTTTTTTTGGCCTGTCAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC009681 unedited CCGGAGTTAGTACGCCTGGGGATCCTTCCCGCTGTTTTGACCTCCATAGAAGAACCGGGA CCGATCCAGCCTCCGGGATCTAGCCTGAGGGCGCGGGACGGATAACAATTTCACACAGGA AACAGCTATGACCATTAGGCCTATTTAGGTGACACTATAGAACAAGTTTGTACAAAAAAG CAGGCTGGTACCGGTCCGGAATTCCCGGGATATCGTCGACCCACGCGTCCGATGACTTCC ACGGGTGGGACGGGAACCTTCCACCCACAGCTATGCCTCTGATTGGTGAATGGTGAAGGT GCCTGTCTAACTTTTCTGTAAAAAGAACCAGCTGCCTCCAGGCAGCCAGCCCTCAAGCAT CACTTACAGGACCAGAGGGACAAGACATGACTGTGATGAGGAGCTGCTTTCGCCAATTTA ACACCAAGAAGAATTGAGGCTGCTTGGGAGGAAGGCCANGAGGAACACGAGACTGAGAGA TGAATTTTCAACAGAGGCTGCAAAGCCTGTGGACTTTAGCCAGCAGACCCTTCTGCCCTC CTTTGCTGGCGACAGCCTCTCAAATGCAGATGGTTGTGCTCCCTTGCCTGCGTTTTACCC TGGTTCTCTGGAGCCAGGTATCAAGGGCCCACGGCCAAGAATTCCACTTTGGGCCCTGCC ACATGAAAGGGGTTGTTCCCCAGAAACTGGGGGAAGCCTTCTGGGCTGGGAAAGACACTA TGCACGCTCAGGATAACATCCCAAGTGCCCGGCTGCTGAACCAGGAGGTTCTGCAGAACC TCTCGGATGCTGAAGAGCTGTACCTTGGCCACACCCGGTTGGAGTTCTATTGAAAACGGT TTCAAAACTACCCCAATAGAACAGTGGAGGGAGGGACTTTAAATCCTTGTTCTATCTGGC GAAAN
Restriction Sites	Please inquire
ACCN	BC009681
Insert Size	1309 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC009681.1, AAH09681.1
RefSeq Size	1309 bp
Locus ID	11009
Protein Families	Druggable Genome, Secreted Protein
Protein Pathways	Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway
Gene Summary	This gene encodes a member of the IL10 family of cytokines. It was identified as a gene induced during terminal differentiation in melanoma cells. The protein encoded by this gene can induce apoptosis selectively in various cancer cells. Overexpression of this gene leads to elevated expression of several GADD family genes, which correlates with the induction of apoptosis. The phosphorylation of mitogen-activated protein kinase 14 (MAPK7/P38), and heat shock 27kDa protein 1 (HSPB2/HSP27) are found to be induced by this gene in melanoma cells, but not in normal immortal melanocytes. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

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