H3FK (HIST1H3H) (BC007518) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	H3/k; H3F1K; H3FK
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC007518 edited CAAAAATTTGAGTCCTTCGCCAATCCGGTTACTGTTGGGTAGGCCTTCAGCATACTTTTG TCCAATCAGCTTCAGACTCTCACTATAAATAAGCGGCTAGCTTTCTCTTTCTCCTGAAGT GAATCTAGCTCTGAAGGCATGGCGCGTACGAAGCAGACTGCTCGCAAGTCCACCGGCGGC AAGGCTCCGCGCAAGCAGCTGGCCACCAAGGCGGCTCGGAAGAGCGCTCCGGCCACCGGC GGTGTCAAGAAGCCCCATCGCTATCGGCCTGGTACAGTGGCTCTCCGCGAGATTCGCCGC TACCAGAAGTCCACCGAGCTGCTGATCAGAAAGCTGCCTTTTCAGCGTCTGGTGCGTGAG ATCGCGCAGGACTTCAAGACCGACTTGCGCTTCCAGAGCTCCGCGGTGATGGCGCTGCAA GAGGCATGCGAGGCCTACCTGGTGGGGCTCTTTGAGGACACCAACCTGTGCGCCATCCAC GCCAAGCGGGTGACTATCATGCCCAAGGACATCCAGCTCGCACGTCGTATCCGCGGCGAG AGGGCTTGAGTCTCAAGGACTCACTGATTACATACCCAAAGGCTCTTTTCAGAGCCACCC ACATGCGCGCTGAAAAGATCTGTTTCTCTCAGGAATTCTTCCTGGTACTTGTTTTGCCTG TAGTAGATAGGGCCCATTTCCAGACGTTATACAATCTGTTTCGTAAGACTCAGCCTATCC CTTTTTGAATGCTAATTTTGGGAGTCTTAACATCTAATAATGTCCGGCATTTTTCCGTAA GCATTGAGTGTAGCCAAAAGTTCCTTCGTGTATTGCTCTCCCATCTCCGCAGCCCGGTTT TGACCGGATGGTGCTTCTAATTTTCTGCTAACCTGTACTGTGGTGTGTGTATATTTCTTG CCAACACGCCAGAAATAAAACTAAGGTTGTACTGAAGTTGGAAAAATTCAGGTTAATGTA GCTCATGCTGGCTAAAGTGAAACGTTCTCTCCCCGCCCCCCGTTCCTAAGCAGTGTTAAG TTTTCTTTGAATTTTTTCAAGCCGGATTTTGGGCCTGCTTAAACCACTTAAATGTAGTTA ATGACAGATGGTTTGAGGTTTAAAAGTCTTCTGGAGAAAGCCCGCCAGAGAACATTCCCT TTGAAGCCCCATGTAAAAATACGTGTGGGAGAGAAAGTGTTTTCTCTGACTTCTGCTGAC AGTGGCTAAAACTCTGAACTGTCAGGAGTATTCAAAATAAGACTGCCTTGTAGGTAAGCC TGTGGTAGTTTTTTTGAGCACAGGATAAAATACTTGAGTCTTTGCTTAAATGTTACTTTC TCAATGAGGCTTTGTATGACTAAATAAAATCTGTATAATCCCCACAAAAAAAAAAAAAAA AAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC007518 unedited NNNTTAGTCAAAATTGTATACGACTCACTAAGGCGGCCGCGAATTCGCACGAGCAAAAAT TGAGTCCTTCGCCAATCCGGTTACTGTTGGGTAGGCCTTCAGCATACTTTTGTCCAATCA GCTTCAGACTCTCACTATAAATAAGCGGCTAGCTTTCTCTTTCTCCTGAAGTGAATCTAG CTCTGAAGGCATGGCGCGTACGAAGCAGACTGCTCGCAAGTCCACCGGCGGCAAGGCTCC GCGCAAGCAGCTGGCCACCAAGGCGGCTCGGAAGAGCGCTCCGGCCACCGGCGGTGTCAA GAAGCCCCATCGCTATCGGCCTGGTACAGTGGCTCTCCGCGAGATTCGCCGCTACCAGAA GTCCACCGAGCTGCTGATCAGAAAGCTGCCTTTTCAGCGTCTGGTGCGTGAGATCGCGCA GGACTTCAAGACCGACTTGCGCTTCCAGAGCTCCGCGGTGATGGCGCTGCAAGAGGCATG CGAGGCCTACCTGGTGGGGCTCTTTGAGGACACCAACCTGTGCGCCATCCACGCCAAGCG GGTGACTATCATGCCCAAGGACATCCAGCTCGCACGTCGTATCCGCGGCGAGAGGGCTTG AGTCTCAAGGACTCACTGATTACATACCCAAAGGCTCTTTTCAGAGCCACCCACATGCGC GCTGAAAAGATCTGTTTCTCTCAGGAATTCTTCCTGGTACTTGTTTTGCCTGTAGTAGAT AGGGCCCATTTCCAGACGTTATACAATCTGTTTCGTAAGACTCAGCCTATCCCTTTTTGA ATGCTAAATTTTGGGAGTCTTAACATCTAAATATGTCCGGCATTTTTCCGTAGCATTGAG TGTAGCCCAAAGTTCCTTCGTGTATTGGCTCTCCATCTNCGCAGCCCGTTTT
Restriction Sites	Please inquire
ACCN	BC007518
Insert Size	1396 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC007518.2
RefSeq Size	1387 bp
Locus ID	8357
Protein Pathways	Systemic lupus erythematosus
Gene Summary	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]

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