United States
Germany
Japan
United Kingdom
China
GRIK1 (NM_000830) Human Untagged Clone
CAT＃: SC122079
GRIK1 (untagged)-Human glutamate receptor, ionotropic, kainate 1 (GRIK1), transcript variant 1
CNY 10,096.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | EAA3; EEA3; GLR5; GluK1; gluR-5; GLUR5 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_000830, the custom clone sequence may differ by one or more nucleotides
ATGGAGCACGGCACACTCCTCGCCCAGCCCGGGCTCTGGACCAGGGACACCAGCTGGGCACTCCTCTATT TCCTCTGCTATATCCTCCCTCAGACCGCCCCGCAAGTACTCAGGATCGGAGGGATTTTTGAAACAGTGGA AAATGAGCCTGTTAATGTTGAAGAATTAGCTTTCAAGTTTGCAGTCACCAGCATTAACAGAAACCGAACC CTGATGCCTAACACCACATTAACCTATGACATCCAGAGAATTAACCTTTTTGATAGTTTTGAAGCCTCGC GGAGAGCATGTGACCAGCTGGCTCTTGGTGTGGCTGCTCTCTTTGGCCCTTCCCATAGCTCCTCCGTCAG TGCTGTGCAGTCTATTTGCAATGCTCTCGAAGTTCCACACATACAGACCCGCTGGAAACACCCCTCGGTG GACAACAAAGATTTGTTTTACATCAACCTTTACCCAGATTATGCAGCTATCAGCAGGGCGATCCTGGATC TGGTCCTCTATTACAACTGGAAAACAGTGACAGTGGTGTATGAAGACAGCACAGGTCTAATTCGTCTACA AGAGCTCATCAAAGCTCCCTCCAGATATAATATTAAAATCAAAATCCGCCAGCTGCCCTCTGGGAATAAA GATGCCAAGCCTTTACTCAAGGAGATGAAGAAAGGCAAGGAGTTCTATGTGATATTTGATTGTTCACATG AAACAGCCGCTGAAATCCTTAAGCAGATTCTGTTCATGGGCATGATGACCGAGTACTATCACTACTTTTT CACAACCCTGGACTTATTTGCTTTGGATCTGGAACTCTATAGGTACAGTGGCGTAAACATGACCGGGTTT CGGCTGCTTAACATTGACAACCCTCACGTGTCATCCATCATTGAGAAGTGGTCCATGGAGAGACTGCAGG CCCCACCCAGGCCCGAGACTGGCCTTTTGGATGGCATGATGACAACTGAAGCGGCTCTGATGTACGATGC TGTGTACATGGTGGCCATTGCCTCGCACCGGGCATCCCAGCTGACCGTCAGCTCCCTGCAGTGCCATAGA CATAAGCCATGGCGCCTCGGACCCAGATTTATGAACCTGATCAAAGAGGCCCGGTGGGATGGCTTGACTG GGCATATCACCTTTAATAAAACCAATGGCTTGAGGAAGGATTTTGATCTGGACATTATTAGTCTCAAAGA GGAAGGAACTGAAAAGGCTGCTGGCGAAGTGTCTAAACACTTGTATAAAGTGTGGAAGAAGATTGGGATT TGGAATTCCAACAGTGGGCTTAACATGACGGACAGCAACAAAGACAAGTCCAGCAATATCACTGATTCAT TGGCCAACAGAACACTCATTGTCACCACCATTCTGGAAGAACCCTATGTTATGTACAGGAAATCTGATAA GCCTCTATATGGAAATGACAGATTTGAAGGATATTGCCTAGACCTGTTGAAAGAATTGTCAAACATCCTG GGTTTCATTTATGATGTTAAACTAGTTCCCGATGGCAAATATGGGGCCCAGAATGACAAAGGGGAGTGGA ACGGGATGGTTAAAGAACTCATAGATCACAGGGCTGACCTGGCAGTGGCTCCTCTTACCATCACCTACGT GCGGGAGAAAGTCATTGACTTCTCCAAACCCTTCATGACCCTAGGCATCAGCATTCTCTACCGGAAGCCC AATGGTACCAATCCAGGCGTTTTCTCCTTCCTCAACCCCCTGTCTCCAGATATTTGGATGTATGTGCTCT TAGCCTGCTTGGGAGTCAGCTGTGTACTCTTTGTGATTGCAAGGTTTACACCCTACGAGTGGTATAACCC CCACCCATGCAACCCTGACTCAGACGTGGTGGAAAACAATTTTACTTTACTAAATAGTTTCTGGTTTGGA GTTGGAGCTCTCATGCAGCAAGGATCAGAGCTGATGCCCAAAGCTCTATCGACCAGAATAGTTGGAGGGA TATGGTGGTTTTTCACCCTAATCATCATTTCATCCTACACGGCCAATCTGGCTGCCTTCTTGACAGTAGA GAGAATGGAATCCCCCATAGATTCGGCAGATGATCTGGCAAAGCAAACCAAGATAGAATATGGGGCGGTT AGAGATGGATCAACAATGACCTTCTTCAAGAAATCAAAAATCTCCACCTATGAGAAGATGTGGGCTTTCA TGAGCAGCAGGCAGCAGACCGCCCTGGTAAGAAACAGTGATGAGGGGATCCAGAGAGTGCTCACCACAGA CTACGCGCTGCTGATGGAGTCCACCAGCATTGAGTATGTGACGCAGAGAAACTGCAACCTCACTCAGATC GGGGGCCTCATTGACTCCAAAGGTTACGGAGTGGGAACACCTATTGGTTCTCCTTACCGGGATAAAATTA CTATTGCTATTCTTCAACTCCAAGAAGAAGGGAAGCTGCATATGATGAAAGAGAAGTGGTGGCGTGGGAA TGGCTGCCCCGAGGAAGACAACAAAGAAGCCAGTGCCCTGGGAGTGGAAAATATTGGAGGCATCTTCATT GTTCTGGCTGCCGGACTGGTCCTTTCTGTATTTGTAGCTATTGGAGAATTCATATACAAATCACGGAAGA ATAATGATATTGAACAGGCTTTTTGTTTCTTTTATGGACTGCAATGTAAGCAAACCCATCCAACCAACTC CACTTCTGGAACTACTTTATCTACGGATTTAGAATGTGGTAAATTAATTCGAGAGGAGAGAGGGATTCGA AAACAGTCCTCAGTTCATACTGTGTAA >OriGene 5' read for NM_000830 unedited
TAAAGTCAGAATTTGTATACGACTCACTATAGGCGGCCGCGAATCGGCACGAGGCGTCCG GCAGCGGCTGTATCTCGGCATGAATTAAGAAGCTAGGAAGATGGAGCACGGCACACTCCT CGCCCAGCCCGGGCTCTGGACCAGGGACACCAGCTGGGCACTCCTCTATTTCCTCTGCTA TATCCTCCCTCAGACCGCCCCGCAAGTACTCAGGATCGGAGGGATTTTTGAAACAGTGGA AAATGAGCCTGTTAATGTTGAAGAATTAGCTTTCAAGTTTGCAGTCACCAGCATTAACAG AAACCGAACCCTGATGCCTAACACCACATTAACCTATGACATCCAGAGAATTAACCTTTT TGATAGTTTTGAAGCCTCGCGGAGAGCATGTGACCAGCTGGCTCTTGGTGTGGCTGCTCT CTTTGGCCCTTCCCATAGCTCCTCCGTCAGTGCTGTGCAGTCTATTTGCAATGCTCTCGA AGTTCCACACATACAGACCCGCTGGAAACACCCCTCGGTGGACAACAAAGATTTGTTTTA CATCAACCTTTACCCAGATTATGCAGCTATCAGCAGGGCGATCCTGGATCTGGTCCTCTA TTACAACTGGAAAACAGTGACAGTGGTGTATGAAGACAGCACAGGTCTAATTCGTCTACA AGAGCTCATCAAAGCTCCCTCCAGATATAATATTAAAATCAAAATCCGCCAGCTGCCCTC TGGGAATAAAGATGCCAAGCCTTTACTCAAGGAGATGAAGAAGGCAAGGAGTTCTATGTG ATATTTGATTGTTCACATGAAACAGCCGCTGAAATCCTTAGCAGATTCTGTTCATGGGCA TGATGACCGAGTCTATCACTACTTTTTCACAACCCTGGACTTATTTGN >OriGene 3' read for NM_000830 unedited
AGAACCTAAGNGAACCGTTGCTGTCTTATTATTTAGGAAGCACACACAACAATTTATTGG GTGAAAAATAAAAAATGCAAAATAATTGAGCATACAAATTTATTTTAAAAGAACTGGCTG TCACTCTCCCAAGTCATATCTATGTGAGCTTTTTAAACTTTTGTATTTCTTATATCTGTA TTCATAATCAGAGTTATGGATATAGATATATGGAAACACATCACACACTCCTCAGAAATC CTTTCTCCAAAAATCTGTAGGGAATGCATCCTTTTTCTTCCTACAGGCGTTTCCTTGGAT CACGCCACAGTCTCTTTTCTCTGAGTTCGTCTCTGATGACAAGTAAGGATACTTGTGAAG GAAGATTTCCCCTTAGTTCTTGACTTTTTCTTTATTTTTTTCTGATTCTTCAGTGAGATT CCCAGTTCTTCCATGATAGCGTTGAAAGAGAGACACTGTTCAATATCATTATTCTTCCGT GATTTGTATATGAATTCTCCAATAGCTACAAATACAGAAAGGACCAGTCCGGCAGCCAGA ACAATGAAGATGCCTCCAATATTTTCCACTCCCAGGGCACTGGCTTCTTTGTTGTCTTCC TCGGGGCAGCCATTCCCACGCCACCACTTCTCTTTCATCATATGCAGCTTCCCTTCTTCT TGGAGTTGAAGAATAGCAATAGTAATTTTATCCCGGTCAGGAGAACCAATAGGTGTTCCC ACTCCGTAACCTTTGGAGTCAATGATGCCCCCGATCTGAGTGATGTTGCAGTTACTCTGC GTCACATACTCGATGCTGGTGGACTCCATCAGCAGCGCGTATTCTGTGGTGAGCACTCTC TGGATCCCCTCATCACTGTTTTCTTACAAGGTCGGTCTGCTGCCTGCTGCTCATGAAAGC CCACATCTGCTATAAAGTGAAA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_000830 |
| Insert Size | 3000 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000830.3, NP_000821.1 |
| RefSeq Size | 3231 bp |
| RefSeq ORF | 2757 bp |
| Locus ID | 2897 |
| UniProt ID | P39086 |
| Domains | lig_chan, ANF_receptor |
| Protein Families | Druggable Genome, Ion Channels: Glutamate Receptors, Transmembrane |
| Protein Pathways | Neuroactive ligand-receptor interaction |
| Gene Summary | Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to the kainate family of glutamate receptors, which are composed of four subunits and function as ligand-activated ion channels. The subunit encoded by this gene is subject to RNA editing (CAG->CGG; Q->R) within the second transmembrane domain, which is thought to alter the properties of ion flow. Alternative splicing, resulting in transcript variants encoding different isoforms, has been noted for this gene. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1), also known as GluR5-1D, contains an alternate 3' terminal exon compared to variant 3. It encodes isoform 1, which is shorter than and has a distinct C-terminus compared to isoform 3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222898 | GRIK1 (Myc-DDK-tagged)-Human glutamate receptor, ionotropic, kainate 1 (GRIK1), transcript variant 1 |
CNY 10,080.00 |
|
| RC222898L1 | Lenti-ORF clone of GRIK1 (Myc-DDK-tagged)-Human glutamate receptor, ionotropic, kainate 1 (GRIK1), transcript variant 1 |
CNY 11,500.00 |
|
| RC222898L2 | Lenti-ORF clone of GRIK1 (mGFP-tagged)-Human glutamate receptor, ionotropic, kainate 1 (GRIK1), transcript variant 1 |
CNY 11,500.00 |
|
| RC222898L3 | Lenti-ORF clone of GRIK1 (Myc-DDK-tagged)-Human glutamate receptor, ionotropic, kainate 1 (GRIK1), transcript variant 1 |
CNY 11,500.00 |
|
| RC222898L4 | Lenti-ORF clone of GRIK1 (mGFP-tagged)-Human glutamate receptor, ionotropic, kainate 1 (GRIK1), transcript variant 1 |
CNY 11,500.00 |
|
| RG222898 | GRIK1 (tGFP-tagged) - Human glutamate receptor, ionotropic, kainate 1 (GRIK1), transcript variant 1 |
CNY 11,680.00 |
