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GAMT (NM_138924) Human Untagged Clone
CAT＃: SC120642
GAMT (untagged)-Human guanidinoacetate N-methyltransferase (GAMT), transcript variant 2
CNY 6,270.00
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热销推荐Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CCDS2; HEL-S-20; PIG2; TP53I2 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_138924 edited
ATGAGCGCCCCCAGCGCGACCCCCATCTTCGCGCCCGGCGAGAACTGCAGCCCCGCGTGG GGGGCGGCGCCCGCGGCCTACGACGCAGCGGACACGCACCTGCGCATCCTGGGCAAGCCG GTGATGGAGCGCTGGGAGACCCCCTATATGCACGCGCTGGCCGCCGCCGCCTCCTCCAAA GGGGGCCGGGTCCTGGAGGTGGGCTTTGGCATGGCCATCGCAGCGTCAAAGGTGCAGGAG GCGCCCATTGATGAGCATTGGATCATCGAGTGCAATGACGGCGTCTTCCAGCGGCTCCGG GACTGGGCCCCACGGCAGACACACAAGGTCATCCCCTTGAAAGGCCTGTGGGAGGATGTG GCACCCACCCTGCCTGACGGTCACTTTGATGGGATCCTGTACGACACGTACCCACTCTCG GAGGAGACCTGGCACACACACCAGTTCAACTTCATCAAGAACCACGCCTTTCGCCTGCTG AAGCCGGGGGGCGTCCTCACCTACTGCAACCTCACCTCCTGGGGGGAGCTGATGAAGTCC AAGTACTCAGACATCACCATCATGTTTGAGGAGACGCAGGTGCCCGCGCTGCTGGAGGCC GGCTTCCGGAGGGAGAACATCCGTACGGAGGTGATGGCGCTGGTCCCACCGGCCGACTGC CGCTACTACGCCTTCCCACAGATGATCACGCCCCTGGTGACCAAAGGCTGA >OriGene 5' read for NM_138924 unedited
NCACTNTNNAGGGCGGCCGCGAATTCGGCACGAGCCCGGGCGGCGCGCGATCGAGGTCGG GTCGCCGTCCAGCCTGCAGCATGAGCGCCCCCAGCGCGACCCCCATCTTCGCGCCCGGCG AGAACTGCAGCCCCGCGTGGGGGGCGGCGCCCGCGGCCTACGACGCAGCGGACACGCACC TGCGCATCCTGGGCAAGCCGGTGATGGAGCGCTGGGAGACCCCCTATATGCACGCGCTGG CCGCCGCCGCCTCCTCCAAGGGGGCCGGGTCCTGGAGGTGGGCTTTGGCATGGCCATCGC AGCGTCAAAGGTGCAGGAGGCGCCCATTGATGAGCATTGGATCATCGAGTGCAATGACGG CGTCTTCCAGCGGCTCCGGGACTGGGCCCCACGGCAGACACACAAGGTCATCCCCTTGAA AGGCCTGTGGGAGGATGTGGCACCCACCCTGCCTGACGGTCACTTTGATGGGATCCTGTA CGACACGTACCCACTCTCGGAGGAGACCTGGCACACACACCAGTTCAACTTCATCAAGAA CCACGCCTTTCGCCTGTTGAAGCCCGGGGTGCGTCCTCACCTACTGGAACCTCACCTCCT GGGGGGAGCCTCAGAAGTCCAAGTACTCAGACCTCACCATTATGTTTGAGGAGACCCACG TGCCCCGTCTGCTGGAAGGCCGTTTTCCGGAGGAGAAAATCCGTACGGAGGTGATGGNTT TGTTTTTACCCGCCGATTTGTGTTCTAACGCTTCCCACAAATTATCTTTTTTTTGTGACT AAAAGCTGATCCCCCCATTTTGTACCCGCCACATTTATGCCCCTTTTTTTGCATTCCTTT TACCGCACTTTTTTTTTTTCCCCTCTCTTTT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_138924 |
| Insert Size | 1250 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_138924.1, NP_620279.1 |
| RefSeq Size | 960 bp |
| RefSeq ORF | 810 bp |
| Locus ID | 2593 |
| UniProt ID | Q14353 |
| Protein Families | Druggable Genome |
| Protein Pathways | Arginine and proline metabolism, Glycine, serine and threonine metabolism, Metabolic pathways |
| Gene Summary | The protein encoded by this gene is a methyltransferase that converts guanidoacetate to creatine, using S-adenosylmethionine as the methyl donor. Defects in this gene have been implicated in neurologic syndromes and muscular hypotonia, probably due to creatine deficiency and accumulation of guanidinoacetate in the brain of affected individuals. Two transcript variants encoding different isoforms have been described for this gene. Pseudogenes of this gene are found on chromosomes 2 and 13. [provided by RefSeq, Feb 2012] Transcript Variant: This variant (2) lacks the 3'-most exon and fails to utilize the 3' splice donor site of the penultimate exon as compared to transcript variant 1. As a result, variant 2 encodes isoform b, which is longer and contains a distinct C-terminus, compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216741 | GAMT (Myc-DDK-tagged)-Human guanidinoacetate N-methyltransferase (GAMT), transcript variant 2 |
CNY 3,990.00 |
|
| RC216741L3 | Lenti ORF clone of Human guanidinoacetate N-methyltransferase (GAMT), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC216741L4 | Lenti ORF clone of Human guanidinoacetate N-methyltransferase (GAMT), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG216741 | GAMT (tGFP-tagged) - Human guanidinoacetate N-methyltransferase (GAMT), transcript variant 2 |
CNY 4,370.00 |
