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Asparagine synthetase (ASNS) (NM_133436) Human Untagged Clone
CAT＃: SC120500
ASNS (untagged)-Human asparagine synthetase (glutamine-hydrolyzing) (ASNS), transcript variant 1
CNY 6,272.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ASNSD; TS11 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC120500 sequence for NM_133436 edited (data generated by NextGen Sequencing)
ATGTGTGGCATTTGGGCGCTGTTTGGCAGTGATGATTGCCTTTCTGTTCAGTGTCTGAGT GCTATGAAGATTGCACACAGAGGTCCAGATGCATTCCGTTTTGAGAATGTCAATGGATAC ACCAACTGCTGCTTTGGATTTCACCGGTTGGCGGTAGTTGACCCGCTGTTTGGAATGCAG CCAATTCGAGTGAAGAAATATCCGTATTTGTGGCTCTGTTACAATGGTGAAATCTACAAC CATAAGAAGATGCAACAGCATTTTGAATTTGAATACCAGACCAAAGTGGATGGTGAGATA ATCCTTCATCTTTATGACAAAGGAGGAATTGAGCAAACAATTTGTATGTTGGATGGTGTG TTTGCATTTGTTTTACTGGATACTGCCAATAAGAAAGTGTTCCTGGGTAGAGATACATAT GGAGTCAGACCTTTGTTTAAAGCAATGACAGAAGATGGATTTTTGGCTGTATGTTCAGAA GCTAAAGGTCTTGTTACATTGAAGCACTCCGCGACTCCCTTTTTAAAAGTGGAGCCTTTT CTTCCTGGACACTATGAAGTTTTGGATTTAAAGCCAAATGGCAAAGTTGCATCCGTGGAA ATGGTTAAATATCATCACTGTCGGGATGAACCCCTGCACGCCCTCTATGACAATGTGGAG AAACTCTTTCCAGGTTTTGAGATAGAAACTGTGAAGAACAACCTCAGGATCCTTTTTAAT AATGCTGTAAAGAAACGTTTGATGACAGACAGAAGGATTGGCTGCCTTTTATCAGGGGGC TTGGACTCCAGCTTGGTTGCTGCCACTCTGTTGAAGCAGCTGAAAGAAGCCCAAGTACAG TATCCTCTCCAGACATTTGCAATTGGCATGGAAGACAGCCCCGATTTACTGGCTGCTAGA AAGGTGGCAGATCATATTGGAAGTGAACATTATGAAGTCCTTTTTAACTCTGAGGAAGGC ATTCAGGCTCTGGATGAAGTCATATTTTCCTTGGAAACTTATGACATTACAACAGTTCGT GCTTCAGTAGGTATGTATTTAATTTCCAAGTATATTCGGAAGAACACAGATAGCGTGGTG ATCTTCTCTGGAGAAGGATCAGATGAACTTACGCAGGGTTACATATATTTTCACAAGGCT CCTTCTCCTGAAAAAGCCGAGGAGGAGAGTGAGAGGCTTCTGAGGGAACTCTATTTGTTT GATGTTCTCCGCGCAGATCGAACTACTGCTGCCCATGGTCTTGAACTGAGAGTCCCATTT CTAGATCATCGATTTTCTTCCTATTACTTGTCTCTGCCACCAGAAATGAGAATTCCAAAG AATGGGATAGAAAAACATCTCCTGAGAGAGACGTTTGAGGATTCCAATCTGATACCCAAA GAGATTCTCTGGCGACCAAAAGAAGCCTTCAGTGATGGAATAACTTCAGTTAAGAATTCC TGGTTTAAGATTTTACAGGAATACGTTGAACATCAGGTTGATGATGCAATGATGGCAAAT GCAGCCCAGAAATTTCCCTTCAATACTCCTAAAACCAAAGAAGGATATTACTACCGTCAA GTCTTTGAACGCCATTACCCAGGCCGGGCTGACTGGCTGAGCCATTACTGGATGCCCAAG TGGATCAATGCCACTGACCCTTCTGCCCGCACGCTGACCCACTACAAGTCAGCTGTCAAA GCTTAG Clone variation with respect to NM_133436.3 629 t=>a >OriGene 5' read for NM_133436 unedited
TGTCTACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGATCACCCTGACCTGCTTA CGCCCAGATTTTCTTCAATCACATCTGAATAAATCACTTGAAGAAAGCTTATAGCTTCAT TGCACCATGTGTGGCATTTGGGCGCTGTTTGGCAGTGATGATTGCCTTTCTGTTCAGTGT CTGAGTGCTATGAAGATTGCACACAGAGGTCCAGATGCATTCCGTTTTGAGAATGTCAAT GGATACACCAACTGCTGCTTTGGATTTCACCGGTTGGCGGTAGTTGACCCGCTGTTTGGA ATGCAGCCAATTCGAGTGAAGAAATATCCGTATTTGTGGCTCTGTTACAATGGTGAAATC TACAACCATAAGAAGATGCAACAGCATTTTGAATTTGAATACCAGACCAAAGTGGATGGT GAGATAATCCTTCATCTTTATGACAAAGGAGGAATTGAGCAAACAATTTGTATGTTGGAT GGTGTGTTTGCATTTGTTTTACTGGATACTGCCAATAAGAAAGTGTTCCTGGGTAGAGAT ACATATGGAGTCAGACCTTTGTTTAAAGCAATGACAGAAGATGGATTTTTGGCTGTATGT TCAGAAGCTAAAGGTCTTGTTACATTGAAGCACTCCGCGACTCCCTTTTTAAAAGTGGAG CCTTTTCTTCCTGGACACTATGAAGTTNTGGATTTAAAGCCCAAATGGCAAGTTGCATCC GTGGAAATGGTTAAATATCATCACTGTCGGGATGAACCCCTGCACGCCCTTCTATGACAT GTGGAGAAACTCTTNTCCAGTTTTGAGATAGAAACTGTGAAGAACAACCTCAGNATCCTT TTTAAATATGCTGTAAAGAAACGTTTGATGACAGAAGAAGGATNGGCTGCCTTTATCAGG GGGCTNNGNACTCACCTTGNTGCTGCCCTCTGTTGAGCAN >OriGene 3' read for NM_133436 unedited
CCGCGGCACGCTATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTAAAATTAGGACTTTTATTTTTTTCACACCCAAGTTAGCCTGAGTTGACTCTT ATTGGTCCCCTATTTACCCACAGTCCCCATCCAACACGAAAAAATATTTGCTTTCACATT ACAGCATAAAGACCACCTAAGCTTTGACAGCTGACTTGGAGGGGGTCAGCGGGCGGGCAA AAGGGTCAGGGGCATTGATCCACTTGGGCATCCAGTAATGGGTCAACCAGTCAGCCCGGC CTGGGTAATGGGGTTCAAAGACTTGACGGGAGTAATATCCTTCTTTGGGTTTAGGAGTAT TGAAGGGAAATTTCTGGGCTGGATTTGCCATCATTGGATCATCAACCTGATGGTCAACGG ATTCCTGGAAAATCTTAAACCAGGAATTCTTAACTGAAGTTATTCCATCACTGAAGGCTT CTTTTGGGCGCCAGAGAATCTCTTTGGGTATCAAATTGGAATCCTCAAACGTCTCTCTCA GGAGAAGTTTTTCTATCCCATTCTTTGGAATTCTCATTTCTGGGGGCAGAGACAAGTAAT AGGAAGAAAATCGATGATCCAAAAATGGGACTCTCAGTTCAAGACCATGGGCAGCAGTAG TTCGATCTGCGCGGAGAACATCAAACAAATAGAGTTCCCTCAGAAGCCTCTCACTCTCCT CCTCGGCTTTTTCAGGAGAAGGAGCCCTTGTGAAAATATATGGAACCCTGCGGTAAGTTC ATCTGATCCTTCTCCAGAGAAGACACCACGCTATCTGTGTTCCTCCGAATATACTCTGGA ATTAAATACCATACCTACTGGAGCACGAACTGTGGGAATGCCATAAGTCTCCAAGGGAAA AATGACTTCCATCCAAAGCCCTGATGCCCTCCTCANAGTAAAAAGGACTCCTTAAGGTCC CTTCC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_133436 |
| Insert Size | 1950 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_133436.1, NP_597680.1 |
| RefSeq Size | 2348 bp |
| RefSeq ORF | 1686 bp |
| Locus ID | 440 |
| UniProt ID | P08243 |
| Domains | GATase_2, Asn_synthase |
| Protein Families | Druggable Genome |
| Protein Pathways | Alanine, aspartate and glutamate metabolism, Metabolic pathways, Nitrogen metabolism |
| Gene Summary | The protein encoded by this gene is involved in the synthesis of asparagine. This gene complements a mutation in the temperature-sensitive hamster mutant ts11, which blocks progression through the G1 phase of the cell cycle at nonpermissive temperature. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010] Transcript Variant: This variant (1) is the longest variant. Variants 1, 2, 3, and 7 encode the same isoform (a). |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC215380 | ASNS (Myc-DDK-tagged)-Human asparagine synthetase (glutamine-hydrolyzing) (ASNS), transcript variant 1 |
CNY 6,264.00 |
|
| RC215380L1 | Lenti ORF clone of Human asparagine synthetase (glutamine-hydrolyzing) (ASNS), transcript variant 1, Myc-DDK-tagged |
CNY 8,664.00 |
|
| RC215380L2 | Lenti ORF clone of Human asparagine synthetase (glutamine-hydrolyzing) (ASNS), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC215380L3 | Lenti ORF clone of Human asparagine synthetase (glutamine-hydrolyzing) (ASNS), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC215380L4 | Lenti ORF clone of Human asparagine synthetase (glutamine-hydrolyzing) (ASNS), transcript variant 1, mGFP tagged |
CNY 8,664.00 |
|
| RG215380 | ASNS (tGFP-tagged) - Human asparagine synthetase (glutamine-hydrolyzing) (ASNS), transcript variant 1 |
CNY 7,864.00 |
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