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NCE2 (UBE2F) (NM_080678) Human Untagged Clone
CAT＃: SC120332
UBE2F (untagged)-Human ubiquitin-conjugating enzyme E2F (putative) (UBE2F), transcript variant 1
CNY 2,400.00
CNY 2,950.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | NCE2 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC120332 sequence for NM_080678 edited (data generated by NextGen Sequencing)
ATGCTAACGCTAGCAAGTAAACTGAAGCGTGACGATGGTCTCAAAGGGTCCCGGACGGCA GCCACAGCGTCCGACTCGACTCGGAGGGTTTCTGTGAGAGACAAATTGCTTGTTAAAGAG GTTGCAGAACTTGAAGCTAATTTACCTTGTACATGTAAAGTGCATTTTCCTGATCCAAAC AAGCTTCATTGTTTTCAGCTAACAGTAACCCCAGATGAGGGTTACTACCAGGGTGGAAAA TTTCAGTTTGAAACTGAAGTTCCCGATGCGTACAACATGGTGCCTCCCAAAGTGAAATGC CTGACCAAGATCTGGCACCCCAACATCACAGAGACAGGGGAAATATGTCTGAGTTTATTG AGAGAACATTCAATTGATGGCACTGGCTGGGCTCCCACAAGAACATTAAAGGATGTCGTT TGGGGATTAAACTCTTTGTTTACTGATCTTTTGAATTTTGATGATCCACTGAATATTGAA GCTGCAGAACATCATTTGCGGGACAAGGAGGACTTCCGGAATAAAGTGGATGACTACATC AAACGTTATGCCAGATGA Clone variation with respect to NM_080678.2 >OriGene 5' read for NM_080678 unedited
TCAGAATTTTGTAATACGACTCACTATAGGGNNCGGCCGCGCAATTCGGCACGAGGCCGC CCCGCCACTTCCGGTCCCGCCGCCGGGAGCCGGTGCGGCTGTGAGGGGCCGCGTCTCGCA GCAGCCGCCCGGACCGGGCATGGTGTTGGGCGCCGGGCCCGCCTCGCCTGTCTCGGGGAG CCCAGGGTAAAGGCAGCAGTAATGCTAACGCTAGCAAGTAAACTGAAGCGTGACGATGGT CTCAAAGGGTCCCGGACGGCAGCCACAGCGTCCGACTCGACTCGGAGGGTTTCTGTGAGA GACAAATTGCTTGTTAAAGAGGTTGCAGAACTTGAAGCTAATTTACCTTGTACATGTAAA GTGCATTTTCCTGATCCAAACAAGCTTCATTGTTTTCAGCTAACAGTAACCCCAGATGAG GGTTACTACCAGGGTGGAAAATTTCAGTTTGAAACTGAAGTTCCCGATGCGTACAACATG GTGCCTCCCAAAGTGAAATGCCTGACCAAGATCTGGCACCCCAACATCACAGAGACAGGG GAAATATGTCTGAGTTTATTGAGAGAACATTCAATTGATGGCACTGGCTGGGCTCCCACA AGAACATTAAAGGATGTCGTTTGGGGATTAAACTCTTTGTTTACTGATCTTTTGAATTTT GATGATCCACTGAATATTGAAGCTGCAGAACATCATTTGCGGGACAAGGAGGACTTNCGG AATAAAGTGGATGACTACATCAAACGTTATGCCAGATGATAAAAGGGGACGNATGCAGGC CCATGGACTGTGTTACAGNTTGTCTCTTACATGAAAACAGCAGAAGTAGCCCCCTCTCCC GTCCTCATGCTCCNTCTCAGTCCCTGGATTGGCCAGTNCTGTGACCATGNTGCCCCTGAG AAGACCATCTTATGACTGCTCATTGTAGATGGAGAAATCACATAAATACANGCAGAAAAG TGTTTGGGCTCTA >OriGene 3' read for NM_080678 unedited
TGGGTAAACAACCTGATTGGGGAAAAGGNAAAACAACCCTCTGGCCTCAATTTTGAGTTC ATTACAACTTCTTAAGAATTTCACCAACAGCCTATACAGTACAAGTTCAAAAAAGTAAAC ATGTTAAGGTAAGCAGACAACTCTTCAGAAGCCCAAACACATTTTCTTGCTGTATTTATG TTGAATTCTCCATCTACAATGAGCAGTCATGTTTTATGGTCTTCTTCAGGGCAACATGGT CACAGGACTGGGGCAATCCAGGGGACTGAGAGGGAGCATGAGGACGGGAGAGGGGGCTAC CTCTTGCTGTTTCATGTTAGAGACAAACTGTAACACAGTCCATGGGCCTGCAATCGTCCC CTTTTATCATCTGGCATAACGTTTGATGTAGTCATCCACTTTATTCCGGAAGTCCTCCTT GTCCCGCAAATGATGTTCTGCAGCTTCAATATTCAGTGGATCATCAAAATTCANAAGATC AGTAAACAAAGAGTTTAATCCCCAAACGACATCCTTTAATGTTCTTGTGGGAGCCCAGCC AGTGCCATCAATTGAATGTTCTCTCAATAAACTCAGACATATTTCCCCTGTCTCTGTGAT GTTGGGGTGCCAGATCTTGGTCAGGCATTTCACTTTGGGAGGCACCATGTTGTACGCATC GGGAAACTCAGTTTCAAACTGAAATTTTCCAACCTGGTAGTTACCCCTCATCTGGGGTTA CTGTTAAGCTGAAACAATGAAAGCTTGTTTGAATCAGGGAAAATGCACCTTACATGTACA GGGTAAATTAGCTTTAAGGTTCTGCAACTCCTTTAACAAGCAATTTGGCTTTTAAGAAAA CCTTTCCAGTCCATTCCGACCCTGGGGCTGCCGTCCGGAACCTTTTGAACCATGGTACGC TTAAGGTTATTGGCTAGCGTTAGCATACTGGCTGGCTTAACCCTGGGCTCCCGAAAACAG GCAAGCGGGCCCG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_080678 |
| Insert Size | 1150 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_080678.1, NP_542409.1 |
| RefSeq Size | 1366 bp |
| RefSeq ORF | 558 bp |
| Locus ID | 140739 |
| UniProt ID | Q969M7 |
| Domains | UBCc |
| Protein Pathways | Ubiquitin mediated proteolysis |
| Gene Summary | Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) encodes the longest isoform (1). Both variants 1 and 2 encode the same isoform (1). |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203942 | UBE2F (Myc-DDK-tagged)-Human ubiquitin-conjugating enzyme E2F (putative) (UBE2F), transcript variant 1 |
CNY 2,400.00 |
|
| RC203942L3 | Lenti ORF clone of Human ubiquitin-conjugating enzyme E2F (putative) (UBE2F), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC203942L4 | Lenti ORF clone of Human ubiquitin-conjugating enzyme E2F (putative) (UBE2F), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG203942 | UBE2F (tGFP-tagged) - Human ubiquitin-conjugating enzyme E2F (putative) (UBE2F), transcript variant 1 |
CNY 4,000.00 |
