Fbx32 (FBXO32) (NM_058229) Human Untagged Clone

CAT#: SC120247

FBXO32 (untagged)-Human F-box protein 32 (FBXO32), transcript variant 1



  "NM_058229" in other vectors (6)

CNY 5,488.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


Anti-FBXO32 Rabbit Polyclonal Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms Fbx32; MAFbx
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC120247 sequence for NM_058229 edited (data generated by NextGen Sequencing)
ATGCCATTCCTCGGGCAGGACTGGCGGTCCCCCGGGCAGAACTGGGTGAAGACGGCCGAC
GGCTGGAAGCGCTTCCTGGATGAGAAGAGCGGCAGTTTCGTGAGCGACCTCAGCAGTTAC
TGCAACAAGGAGGTATACAATAAGGAGAATCTTTTCAACAGCCTGAACTATGATGTTGCA
GCCAAGAAGAGAAAGAAGGACATGCTGAATAGCAAAACCAAAACTCAGTATTTCCACCAA
GAAAAATGGATCTATGTTCACAAAGGAAGTACTAAAGAGCGCCATGGATATTGCACCCTG
GGGGAAGCTTTCAACAGACTGGACTTCTCAACTGCCATTCTGGATTCCAGAAGATTTAAC
TACGTGGTCCGGCTGTTGGAGCTGATAGCAAAGTCACAGCTCACATCCCTGAGTGGCATC
GCCCAAAAGAACTTCATGAATATTTTGGAAAAAGTGGTACTGAAAGTCCTTGAAGACCAG
CAAAACATTAGACTAATAAGGGAACTACTCCAGACCCTCTACACATCCTTATGTACACTG
GTCCAAAGAGTCGGCAAGTCTGTGCTGGTCGGGAACATTAACATGTGGGTGTATCGGATG
GAGACGATTCTCCACTGGCAGCAGCAGCTGAACAACATTCAGATCACCAGGCCTGCCTTC
AAAGGCCTCACCTTCACTGACCTGCCTTTGTGCCTACAACTGAACATCATGCAGAGGCTG
AGCGACGGGCGGGACCTGGTCAGCCTGGGCCAGGCTGCCCCCGACCTGCACGTGCTCAGC
GAAGACCGGCTGCTGTGGAAGAAACTCTGCCAGTACCACTTCTCCGAGCGGCAGATCCGC
AAACGATTAATTCTGTCAGACAAAGGGCAGCTGGATTGGAAGAAGATGTATTTCAAACTT
GTCCGATGTTACCCAAGGAAAGAGCAGTATGGAGATACCCTTCAGCTCTGCAAACACTGT
CACATCCTTTCCTGGAAGGGCACTGACCATCCGTGCACTGCCAATAACCCAGAGAGCTGC
TCCGTTTCACTTTCACCCCAGGACTTTATCAACTTGTTCAAGTTCTGA

Clone variation with respect to NM_058229.3
>OriGene 5' read for NM_058229 unedited
GATTTTGTAATACGACTCACTATAGGGCGGCCGCGCTGCAACGCTTGAGATCCTCTCCGC
GCCCGCCACCCCGCAGGGTGCCCCGCGCCGTTCCCGCCGCCCCGCCGCCCCCGTCGCGGG
CCCCTGCACCCCGAGCATCCGCCCCGGGTGGCACGTCCCCGAGCCCACCAGGCCGGCCCC
GTCTCCCCATCCGTCTAGTCCGCTCGCGGTGCCATGCCATTCCTCGGGCAGGACTGGCGG
TCCCCCGGGCAGAACTGGGTGAAGACGGCCGACGGCTGGAAGCGCTTCCTGGATGAGAAG
AGCGGCAGTTTCGTGAGCGACCTCAGCAGTTACTGCAACAAGGAGGTATACAATAAGGAG
AATCTTTTCAACAGCCTGAACTATGATGTTGCAGCCAAGAAGAGAAAGAAGGACATGCTG
AATAGCAAAACCAAAACTCAGTATTTCCACCAAGAAAAATGGATCTATGTTCACAAAGGA
AGTACTAAAGAGCGCCATGGATATTGCACCCTGGGGGAAGCTTTCAACAGACTGGACTTC
TCAACTGCCATTCTGGATTCCAGAAGATTTAACTACGTGGTCCGGCTGTTGGAGCTGATA
GCAAAGTCACAGCTCACATCCCTGAGTGGCATCGCCCAAAAGAACTTCATGAATATTTTG
GAAAAAGTGGTACTGAAAGTCCTTGAAGACCAGCAAAACATTAGACCTAATAGGGAACTA
CTCCAGACCCTCTACACATNCTTATGTACACTGGTCCAAAGAGTCGGNCAGTCTGTGCTG
GTCGGGAACATTACATGGNGGGTGTATCGGATGGAGACAATTCTCACTGGCAGCAGCAGC
TGAACACATTCAGATCACCAGCCTGGCTTCAAGGCCTCACCTTCACTGACCTGNCTTNNG
TGGCTACACTGACTCATGCANAGGCTGACGACGGNCGGNACCTGGTCAGNCTGGGCCAGC
TGNCCCCGACTGCCGTGCTTANNGAGACCGCTGNTTG
>OriGene 3' read for NM_058229 unedited
NGGCCTATTATGTACCGCGCCGCATTCTANGATCGATTTTTTTTTTTTTTTTTTTTGTTA
AAAAAAAATTTATTTACAGTATTTTGCTTTTCCATACCAATTCTAGTGAGAAGTTCACAT
TCTTAAATTCCCAGTCAGCAACTGCATTTCTCCCCTCCAATGTCCTCTCCATGACTTATC
TCTGAAGTTTCGAGCCAATGTTTAAAATGTACACTATTTACAAATGAAGTGTCCAAATGC
CATATTCCCAGCTCTCCAGTCAGCAGGGGGACCCTTCTGAAGTGTTGTCATGTGCTGGGA
TTCAGAACTTGAACAAGTTGATAAAGTCCTGGGGTGAAAGTGAAACGGAGCAGCTCTCTG
GGTTATTGGCAGTGCACGGATGGTCAGTGCCCTTCCAGGAAAGGATGTGACAGTGTTTGC
AGAGCTGAAGGGTATCTCCATACTGCTCTTTCCTTGGGTAACATCGGACAAGTTTGAAAT
ACATCTTCTTCCAATCCAGCTGCCCTTTGTCTGACAGAATTAATCGTTTGCGGATCTGCC
GCTCGGAGAAGTGGTACTGGCAGAGTTTCTTCCACAGCAGCCGGTCTTCGCTGAGCACGT
GCAGGTCGGGGGCAGCCTGGCCCATGCTGACCAGGTCCCGCCCGTCGCTCAGCCTCTGCA
TGATGTTCAGTTGTAAGCACAAAGGCAGGTCAGTGAAAGTGATGCCTTTGAAGGCAGGCC
TGGTGATCTGAATGTTGTTCAACTGCTGCTGCCAGTGGAGAATTGTCTCCATCCGATACA
CCCACATGTTAATGTTTCCCGACCAGCACAGACTTGCCGACTCTTTGGACCAGTGTACAT
AAGATGTGGTAAAGGGTCTGGAATAA
Restriction Sites NotI-NotI     
ACCN NM_058229
Insert Size 1500 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_058229.2, NP_478136.1
RefSeq Size 1530 bp
RefSeq ORF 1068 bp
Locus ID 114907
UniProt ID Q969P5
Domains F-box
Gene Summary This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class and contains an F-box domain. This protein is highly expressed during muscle atrophy, whereas mice deficient in this gene were found to be resistant to atrophy. This protein is thus a potential drug target for the treatment of muscle atrophy. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011]
Transcript Variant: This variant (1) has a longer and alternate 5' terminal sequence containing an in-frame translation start codon, as compared to variant 2. It encodes a longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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