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GPCR MRGX2 (MRGPRX2) (NM_054030) Human Untagged Clone
CAT＃: SC120221
MRGPRX2 (untagged)-Human MAS-related GPR, member X2 (MRGPRX2)
CNY 2,400.00
CNY 2,950.00
| Cited in 2 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | MGRG3; MRGX2 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_054030, RT-PCR generated
TTCTGAGCATGGATCCAACCACCCCGGCCTGGGGAACAGAAAGTACAACAGTGAATGGAA ATGACCAAGCCCTTCTTCTGCTTTGTGGCAAGGAGACCCTGATCCCGGTCTTCCTGATCC TTTTCATTGCCCTGGTTGGGCTGGTAGGAAACGGGTTTGTGCTCTGGCTCCTGGGCTTCC GCATGCGCAGGAACGCCTTCTCTGTCTACGTCCTCAGCCTGGCCGGGGCCGACTTCCTCT TCCTCTGCTTCCAGATTATAAATTGCCTGGTGTACCTCAGTAACTTCTTCTGTTCCATCT CCATCAATTTCCCTAGCTTCTTCACCACTGTGATGACCTGTGCCTACCTTGCAGGCCTGA GCATGCTGAGCACCGTCAGCACCGAGCGCTGCCTGTCCGTCCTGTGGCCCATCTGGTATC GCTGCCGCCGCCCCAGACACCTGTCAGCGGTCGTGTGTGTCCTGCTCTGGGCCCTGTCCC TACTGCTGAGCATCTTGGAAGGGAAGTTCTGTGGCTTCTTATTTAGTGATGGTGACTCTG GTTGGTGTCAGACATTTGATTTCATCACTGCAGCGTGGCTGATTTTTTTATTCATGGTTC TCTGTGGGTCCAGTCTGGCCCTGCTGGTCAGGATCCTCTGTGGCTCCAGGGGTCTGCCAC TGACCAGGCTGTACCTGACCATCCTGCTCACAGTGCTGGTGTTCCTCCTCTGCGGCCTGC CCTTTGGCATTCAGTGGTTCCTAATATTATGGATCTGGAAGGATTCTGATGTCTTATTTT GTCATATTCATCCAGTTTCAGTTGTCCTGTCATCTCTTAACAGCAGTGCCAACCCCATCA TTTACTTCTTCGTGGGCTCTTTTAGGAAGCAGTGGCGGCTGCAGCAGCCGATCCTCAAGC TGGCTCTCCAGAGGGCTCTGCAGGACATTGCTGAGGTGGATCACAGTGAAGGATGCTTCC GTCAGGGCACCCCGGAGATGTCGAGAAGCAGTCTGGTGTAG >OriGene 5' read for NM_054030 unedited
TTGGGCAAATTCGGGATTTGTAAACTATCATATAGGCGGCCGCCAGTGTGATGGATATCT GCAGAATTCGGCTTTTCTGAGCATGGATCCAACCACCCCGGCCTGGGGAACAGAAAGTAC AACAGTGAATGGAAATGACCAAGCCCTTCTTCTGCTTTGTGGCAAGGAGACCCTGATCCC GGTCTTCCTGATCCTTTTCATTGCCCTGGTTGGGCTGGTAGGAAACGGGTTTGTGCTCTG GCTCCTGGGCTTCCGCATGCGCAGGAACGCCTTCTCTGTCTACGTCCTCAGCCTGGCCGG GGCCGACTTCCTCTTCCTCTGCTTCCAGATTATAAATTGCCTGGTGTACCTCAGTAACTT CTTCTGTTCCATCTCCATCAATTTCCCTAGCTTCTTCACCACTGTGATGACCTGTGCCTA CCTTGCAGGCCTGAGCATGCTGAGCACCGTCAGCACCGAGCGCTGCCTGTCCGTCCTGTG GCCCATCTGGTATCGCTGCCGCCGCCCCAGACACCTGTCAGCGGTCGTGTGTGTCCTGCT CTGGGCCCTGTCCCTACTGCTGAGCATCTTGGAAGGGAAGTTCTGTGGCTTCTTATTTAG TGATGGTGACTCTGGTTGGTGTCAGACATTTGATTTCATCACTGCAGCGTGGCTGATTTT TTTATTCATGGTTCTCTGTGGGTCCAGTCTGGCCCTGCTGGTCAGGATCCTCTGTGGCTC CAGGGGTCTGCCACTGACCAGGCTGTACCTGACCATCCTGCTCACAGTGCTTGGTGTTCC TCCTCTGCGGCCTGCCCTTTGGCATTCAGTGGTTCCTAATATATGGATTCTGGAAGATTC TGATGTCTTATTTTGTCATATTCATCCAGTT >OriGene 3' read for NM_054030 unedited
NGGCGCCATTGGGNGATAGGCAACTTGCCAGGGCCAGTAAAGCACTGGGGNAGGGTCACA GGGCAGCCACCCGGGCTCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCG ACAAGCTTGATATCGGTACCGAGCTCGGATCCACTAGTAACGGCCGCCAGTGTGCTGGAA TTCGGCTTCTACACCAGACTGCTTCTCGACATCTCCGGGGTGCCCTGACGGAAGCATCCT TCACTGTGATCCACCTCAGCAATGTCCTGCAGAGCCCTCTGGAGAGCCAGCTTGAGGATC GGCTGCTGCAGCCGCCACTGCTTCCTAAAAGAGCCCACGAAGAAGTAAATGATGGGGTTG GCACTGCTGTTAAGAGATGACAGGACAACTGAAACTGGATGAATATGACAAAATAAGACA TCAGAATCCTTCCAGATCCATAATATTAGGAACCACTGAATGCCAAAGGGCAGGCCGCAG AGGAAGAACACCAGCACTGTGAGCAGGATGGTCAGGTACAGCCTGGTCAGTGGCAGACCC CTGGAGCCACAGAGGATCCTGACCAGCAGGGCCAGACTGGACCCACAGAGAACCATGAAT AAAAAAATCAGCCACGCTGCAGTGATGAAATCAAATGTCTGACACCAACCAGAGTCACCA TCACTAAATAAGAAGCCACAGAACTTCCCTTCCAAGATGCTCAGCAGTAGGGACAGGGCC CAGAGCATGACACACACGACCGCTGACAGGTGTCTGGGGCGGCGGCAGCGATACCAGATG GGCCACAGGACGGACAGGCAGCGCTCGGTGCTGACAGTGCTCANCATGCTCAGGCCTGCA AGGTAGGCACAGGTCATCACAGTGGTGGAGAAGCTAGGGGAATTGATTGGAGATG |
| Restriction Sites | Please inquire |
| ACCN | NM_054030 |
| Insert Size | 1700 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_054030.2, NP_473371.1 |
| RefSeq Size | 2130 bp |
| RefSeq ORF | 993 bp |
| Locus ID | 117194 |
| UniProt ID | Q96LB1 |
| Protein Families | Druggable Genome, GPCR, Transmembrane |
| Gene Summary | Mast cell-specific receptor for basic secretagogues, i.e. cationic amphiphilic drugs, as well as endo- or exogenous peptides, consisting of a basic head group and a hydrophobic core (PubMed:25517090). Recognizes and binds small molecules containing a cyclized tetrahydroisoquinoline (THIQ), such as non-steroidal neuromuscular blocking drugs (NMBDs), including tubocurarine and atracurium. In response to these compounds, mediates pseudo-allergic reactions characterized by histamine release, inflammation and airway contraction (By similarity). Acts as a receptor for a number of other ligands, including peptides and alkaloids, such as cortistatin-14, proadrenomedullin N-terminal peptides PAMP-12 and, at lower extent, PAMP-20, antibacterial protein LL-37, PMX-53 peptide, beta-defensins, and complanadine A.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein. |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Mast cell degranulation via MRGPRX2 by isolated human albumin fragments
,Karhu, T;Akiyama, K;Vuolteenaho, O;Bergmann, U;Naito, T;Tatemoto, K;Herzig, KH;,
Biochim. Biophys. Acta
,PubMed ID 28844982
[MRGPRX2]
|
|
PMX-53 as a Dual CD88 Antagonist and an Agonist for Mas-Related Gene 2 (MrgX2) in Human Mast Cells
,Hariharan Subramanian, Sakeen W. Kashem, Sarah J. Collington, Hongchang Qu, John D. Lambris, and Hydar Ali,
Mol. Pharmacol., Jun 2011; 79: 1005 - 1013
[MRGPRX2]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209033 | MRGPRX2 (Myc-DDK-tagged)-Human MAS-related GPR, member X2 (MRGPRX2) |
CNY 2,400.00 |
|
| RC209033L3 | Lenti ORF clone of Human MAS-related GPR, member X2 (MRGPRX2), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC209033L4 | Lenti ORF clone of Human MAS-related GPR, member X2 (MRGPRX2), mGFP tagged |
CNY 4,800.00 |
|
| RG209033 | MRGPRX2 (tGFP-tagged) - Human MAS-related GPR, member X2 (MRGPRX2) |
CNY 4,000.00 |
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