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GLDC (NM_000170) Human Untagged Clone
CAT＃: SC120090
GLDC (untagged)-Human glycine dehydrogenase (decarboxylating) (GLDC), nuclear gene encoding mitochondrial protein
CNY 10,664.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | GCE; GCSP; HYGN1 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_000170 edited
GGCACGAGGGCAGCGCCCATTCATTGCCCGCGAGCGTCCATCCATCTGTCCGGCCGACTG TCCAGCGAAAGGGGCTCCAGGCCGGGCGCAGCCGCCACCCGGGGGACCGAGGCCAGGAGA GGGGCCAAGAGCGCGGCTGACCCTTGCGGGCCGGGGCAGGGGACGGTGGCCGCGGCCATG CAGTCCTGTGCCAGGGCGTGGGGGCTGCGCCTGGGCCGCGGGGTCGGGGGCGGCCGCCGC CTGGCTGGGGGATCGGGGCCGTGCTGGGCGCCGCGGAGCCGGGACAGCAGCAGTGGCGGC GGGGACAGCGCCGCGGCTGGGGCCTCGCGCCTCCTGGAGCGCCTTCTGCCCAGACACGAC GACTTCGCTCGGAGGCACATCGGCCCTGGGGACAAAGACCAGAGAGAGATGCTGCAGACC TTGGGGCTGGCGAGCATTGATGAATTGATCGAGAAGACGGTCCCTGCCAACATCCGTTTG AAAAGACCCTTGAAAATGGAAGACCCTGTTTGTGAAAATGAAATCCTTGCAACTCTGCAT GCCATTTCAAGCAAAAACCAGATCTGGAGATCGTATATTGGCATGGGCTATTATAACTGC TCAGTGCCACAGACGATTTTGCGGAACTTACTGGAGAACTCAGGATGGATCACCCAGTAT ACTCCATACCAGCCTGAGGTGTCTCAGGGGAGGCTGGAGAGTTTACTCAACTACCAGACC ATGGTGTGTGACATCACAGGCCTGGACATGGCCAATGCATCCCTGCTGGATGAGGGGACT GCAGCCGCAGAGGCACTGCAGCTGTGCTACAGACACAACAAGAGGAGGAAATTTCTCGTT GATCCCCGTTGCCACCCACAGACAATAGCTGTTGTCCAGACTCGAGCCAAATATACTGGA GTCCTCACTGAGCTGAAGTTACCCTGTGAAATGGACTTCAGTGGAAAAGATGTCAGTGGA GTGTTGTTCCAGTACCCAGACACGGAGGGGAAGGTGGAAGACTTTACGGAACTCGTGGAG AGAGCTCATCAGAGTGGGAGCCTGGCCTGCTGTGCTACTGACCTTTTAGCTTTGTGCATC TTGAGGCCACCTGGAGAATTTGGGGTAGACATCGCCCTGGGCAGCTCCCAGAGATTTGGA GTGCCACTGGGCTATGGGGGACCCCATGCAGCATTTTTTGCTGTCCGAGAAAGCTTGGTG AGAATGATGCCTGGAAGAATGGTGGGGGTAACAAGAGATGCCACTGGGAAAGAAGTGTAT CGTCTTGCTCTTCAAACCAGGGAGCAACACATTCGGAGAGACAAGGCTACCAGCAACATC TGTACAGCTCAGGCCCTCTTGGCGAATATGGCTGCCATGTTTGCAATCTACCATGGTTCC CATGGGCTGGAGCATATTGCTAGGAGGGTACATAATGCCACTTTGATTTTGTCAGAAGGT CTCAAGCGAGCAGGGCATCAACTCCAGCATGACCTGTTCTTTGATACCTTGAAGATTCAG TGTGGCTGCTCAGTGAAGGAGGTCTTGGGCAGGGCCGCTCAGCGGCAGATCAATTTTCGG CTTTTTGAGGATGGCACACTTGGTATTTCTCTTGATGAAACAGTCAATGAAAAAGATCTG GACGATTTGTTGTGGATCTTTGGTTGTGAGTCATCTGCAGAACTGGTTGCTGAAAGCATG GGAGAGGAGTGCAGAGGTATTCCAGGGTCTGTGTTCAAGAGGACCAGCCCGTTCCTCACC CATCAAGTGTTCAACAGCTACCACTCTGAAACAAACATTGTCCGGTACATGAAGAAACTG GAAAATAAAGACATTTCCCTTGTTCACAGCATGATTCCACTGGGATCCTGCACCATGAAA CTGAACAGTTCGTCTGAACTCGCACCTATCACATGGAAAGAATTTGCAAACATCCACCCC TTTGTGCCTCTGGATCAAGCTCAAGGATATCAGCAGCTTTTCCGAGAGCTTGAGAAGGAT TTGTGTGAACTCACAGGTTATGACCAGGTCTGTTTCCAGCCAAACAGCGGAGCCCAGGGA GAATATGCTGGACTGGCCACTATCCGAGCCTACTTAAACCAGAAAGGAGAGGGGCACAGA ACGGTTTGCCTCATTCCGAAATCAGCACATGGGACCAACCCAGCAAGTGCCCACATGGCA GGCATGAAGATTCAGCCTGTGGAGGTGGATAAATATGGGAATATCGATGCAGTTCACCTC AAGGCCATGGTGGATAAGCACAAGGAGAACCTAGCAGCTATCATGATTACATACCCATCC ACCAATGGGGTGTTTGAAGAGAACATCAGTGACGTGTGTGACCTCATCCATCAACATGGA GGACAGGTCTACCTAGACGGGGCAAATATGAATGCTCAGGTGGGAATCTGTCGCCCTGGA GACTTCGGGTCTGATGTCTCGCACCTAAATCTTCACAAGACCTTCTGCATTCCCCACGGA GGAGGTGGTCCTGGCATGGGGCCCATCGGAGTGAAGAAACATCTCGCCCCGTTTTTGCCC AATCATCCCGTCATTTCACTAAAGCGGAATGAGGATGCCTGTCCTGTGGGAACCGTCAGT GCGGCCCCATGGGGCTCCAGTTCCATCTTGCCCATTTCCTGGGCTTATATCAAGATGATG GGAGGCAAGGGTCTTAAACAAGCCACGGAAACTGCGATATTAAATGCCAACTACATGGCC AAGCGATTAGAAACACACTACAGAATTCTTTTCAGGGGTGCAAGAGGTTATGTGGGTCAT GAATTTATTTTGGACACGAGACCCTTCAAAAAGTCTGCAAATATTGAGGCTGTGGATGTG GCCAAGAGACTCCAGGATTATGGATTTCACGCCCCTACCATGTCCTGGCCTGTGGCAGGG ACCCTCATGGTGGAGCCCACTGAGTCGGAGGACAAGGCAGAGCTGGACAGATTCTGTGAT GCCATGATCAGCATTCGGCAGGAAATTGCTGACATTGAGGAGGGCCGCATCGACCCCAGG GTCAATCCGCTGAAGATGTCTCCACACTCCCTGACCTGCGTTACATCTTCCCACTGGGAC CGGCCTTATTCCAGAGAGGTGGCAGCATTCCCACTCCCCTTCGTGAAACCAGAGAACAAA TTCTGGCCAACGATTGCCCGGATTGATGACATATATGGAGATCAGCACCTGGTTTGTACC TGCCCACCCATGGAAGTTTATGAGTCTCCATTTTCTGAACAAAAGAGGGCGTCTTCTTAG TCCTCTCTCCCTAAGTTTAAAGGACTGATTTGATGCCTCTCCCCAGAGCATTTGATAAGC AAGAAAGATTTCATCTCCCACCCCAGCCTCAAGTAGGAGTTTTATATACTGTGTATATCT CTGTAATCTCTGTCAAGGTAAATGTAAATACAGTAGCTGGAGGGAGTCGAAGCTGATGGT TGGAAGACGGATTTGCTTTGGTATTCTGCTTCCACATGTGCCAGTTGCCTGGATTGGGAG CCATTTTGTGTTTTGCGTAGAAAGTTTTAGGAACTTTAACTTTTAATGTGGCAAGTTTGC AGATGTCATAGAGGCTATCCTGGAGACTTAATAGACATTTTTTTGTTCCAAAAGAGTCCA TGTGGACTGTGCCATCTGTGGGAAATCCCAGGGCAAATGTTTACATTTTGTATACCCTGA AGAACTCTTTTTCCTCTAATATGCCTAATCTGTAATCACATTTCTGAGTGTTTTCCTCTT TTTCTGTGTGAGGTTTTTTTTTTTTAATCTGCATTTATTAGTATTCTAATAAAAGCTTTT GATCGGAAAAAAAAAAAAAAAAAAC >OriGene 5' read for NM_000170 unedited
GGTCACAATTGTATACGACTCACTATAGGCGGCCGCGATTCGGCACGAGGGCAGCGCCCA TTCATTGCCCGCGAGCGTCCATCCATCTGTCCGGCCGACTGTCCAGCGAAAGGGGCTCCA GGCCGGGCGCAGCCGCCACCCGGGGGACCGAGGCCAGGAGAGGGGCCAAGAGCGCGGCTG ACCCTTGCGGGCCGGGGCAGGGGACGGTGGCCGCGGCCATGCAGTCCTGTGCCAGGGCGT GGGGGCTGCGCCTGGGCCGCGGGGTCGGGGGCGGCCGCCGCCTGGCTGGGGGATCGGGGC CGTGCTGGGCGCCGCGGAGCCGGGACAGCAGCAGTGGCGGCGGGGACAGCGCCGCGGCCT GGGGGCCTCGCGCCTCCTGGAGCGCCTTCTGCCCAGACACGACGACTTCGCTCGGAGGCA CATCGGCCCTGGGGACAAAGACCAGAGAGAGATGCTGCAGACCTTGGGGCTGGCGAGCAT TGATGAATTGATCGAGAAGACGGTCCCTGCCAACATCCGTTTGAAAAGACCCTTGAAAAT GGAAGACCCTGTTTGTGAAAATGAAATCCTTGCAACTCTGCATGCCATTTCAAGCAAAAA CCAGATCTGGAGATCGTATATTGGCATGGGCTATTAATCGGGGGTTCAATGCCACAGACG ATTTTGCGGAACTTACTGGAGAACTCANGATGGATCACCCAGTATACTCCATTACCAGCC TGAGTGTCTCAGGNGAGGCTGNGAGAGTTACTCAACTACCAGACCATGGGTGTGTGACAT CACAGGCCTGGACATGGCCAATGCATCCCTGCTGGATGAAGGGGACTGCAGCCGCANAAG CACTGCAGCTGTGCTACAGACACAACAAGAGGGAGGAAATTTCTCGTTGATCCCCGTTGC CACCACAGACAATACCTGTTGTTCAGACTCGAGCCAAATTC >OriGene 3' read for NM_000170 unedited
TATGGACCGCGGCACGCAATCTAGTATCGAGTTTTTTTTTTTTTTTTTTCCGATCAAAAG CTTTTATTAGAATACTAATAAATGCAGATTAAAAAAAAAAAACCTCACACAGAAAAAGAG GAAAACACTCAGAAATGTGATTACAGATTAGGCATATTAGAGGAAAAAGAGTTCTTCAGG GTATACAAAATGTAAACATTTGCCCTGGGATTTCCCACAGATGGCACAGTCCACATGGAC TCTTTTGGAACAAAAAAATGTCTATTAAGTCTCCAGGATAGCCTCTATGACATCTGCAAA CTTGCCACATTAAAAGTTAAAGTTCCTAAAACTTTCTACGCAAAACACAAAATGGCTCCC AATCCAGGCAACTGGCACATGTGGAAGCAGAATACCAAAGCAAATCCGTCTTCCAACCAT CNAGCTTCGACTTCCTTCCCACCTACTGGAATTTAACCTTTTAACCCTTGGGGCGGGGTT TTTCCCCGGGTATTTTCCCCCGGGTTTTTTAAAACCCCCCTTTTGGGGGGGGGGGGGGGG AAAAAAAATTTTTTTGGTCTTAAAAGCGCCCGGGGGGGAGGGGGCAAAAAAACCCCCTCT TTTAACCTGTTTGGGGGGGAGGGAGGGCCCTTTAAAAAACCCCCCCCCTTTTTTTTTTTT TAAAAAAAAGGGGGGGGGCTCTCTTCCTTCCCTCCCCCCCGGGGGGGGGGGGGGGGGGGA AAAAAAAAAAGGGGGGGGGGGCCCTCTTTTATTTTTTAAAACTTCCGGCGGGCCTGTGGG GGGCCAAAAAAATATTTTTTTTTTTTTTTTTCTTGGGGGGGGGGGGGGGGGGGCCCCCCC CCCCTCTTTATAAATAAACCCCCCCCCCCCCAAAAAAA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_000170 |
| Insert Size | 3700 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000170.1, NP_000161.1 |
| RefSeq Size | 3783 bp |
| RefSeq ORF | 3063 bp |
| Locus ID | 2731 |
| UniProt ID | P23378 |
| Domains | GDC-P |
| Protein Families | Druggable Genome |
| Protein Pathways | Glycine, serine and threonine metabolism, Metabolic pathways |
| Gene Summary | Degradation of glycine is brought about by the glycine cleavage system, which is composed of four mitochondrial protein components: P protein (a pyridoxal phosphate-dependent glycine decarboxylase), H protein (a lipoic acid-containing protein), T protein (a tetrahydrofolate-requiring enzyme), and L protein (a lipoamide dehydrogenase). The protein encoded by this gene is the P protein, which binds to glycine and enables the methylamine group from glycine to be transferred to the T protein. Defects in this gene are a cause of nonketotic hyperglycinemia (NKH).[provided by RefSeq, Jan 2010] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Nonketotic hyperglycinemia: Functional assessment of missense variants in GLDC to understand phenotypes of the disease
,Bravo-Alonso, I;Navarrete, R;Arribas-Carreira, L;Perona, A;Abia, D;Couce, ML;García-Cazorla, A;Morais, A;Domingo, R;Ramos, MA;Swanson, MA;Van Hove, JL;Ugarte, M;Pérez, B;Pérez-Cerdá, C;Rodríguez-Pombo, P;,
Hum. Mutat
,PubMed ID 28244183
[GLDC]
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211292 | GLDC (Myc-DDK-tagged)-Human glycine dehydrogenase (decarboxylating) (GLDC), nuclear gene encoding mitochondrial protein |
CNY 10,656.00 |
|
| RC211292L1 | Lenti-ORF clone of GLDC (Myc-DDK-tagged)-Human glycine dehydrogenase (decarboxylating) (GLDC), nuclear gene encoding mitochondrial protein |
CNY 13,056.00 |
|
| RC211292L2 | Lenti-ORF clone of GLDC (mGFP-tagged)-Human glycine dehydrogenase (decarboxylating) (GLDC), nuclear gene encoding mitochondrial protein |
CNY 12,070.00 |
|
| RC211292L3 | Lenti-ORF clone of GLDC (Myc-DDK-tagged)-Human glycine dehydrogenase (decarboxylating) (GLDC), nuclear gene encoding mitochondrial protein |
CNY 12,070.00 |
|
| RC211292L4 | Lenti-ORF clone of GLDC (mGFP-tagged)-Human glycine dehydrogenase (decarboxylating) (GLDC), nuclear gene encoding mitochondrial protein |
CNY 13,056.00 |
|
| RG211292 | GLDC (tGFP-tagged) - Human glycine dehydrogenase (decarboxylating) (GLDC), nuclear gene encoding mitochondrial protein |
CNY 12,256.00 |
