GAMT (NM_000156) Human Untagged Clone

CAT#: SC120079

GAMT (untagged)-Human guanidinoacetate N-methyltransferase (GAMT), transcript variant 1



  "NM_000156" in other vectors (7)

CNY 2,400.00

CNY 2,950.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
GAMT Rabbit polyclonal Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

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TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms CCDS2; HEL-S-20; PIG2; TP53I2
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC120079 sequence for NM_000156 edited (data generated by NextGen Sequencing)
ATGAGCGCCCCCAGCGCGACCCCCATCTTCGCGCCCGGCGAGAACTGCAGCCCCGCGTGG
GGGGCGGCGCCCGCGGCCTACGACGCAGCGGACACGCACCTGCGCATCCTGGGCAAGCCG
GTGATGGAGCGCTGGGAGACCCCCTATATGCACGCGCTGGCCGCCGCCGCCTCCTCCAAA
GGGGGCCGGGTCCTGGAGGTGGGCTTTGGCATGGCCATCGCAGCGTCAAAGGTGCAGGAG
GCGCCCATTGATGAGCATTGGATCATCGAGTGCAATGACGGCGTCTTCCAGCGGCTCCGG
GACTGGGCCCCACGGCAGACACACAAGGTCATCCCCTTGAAAGGCCTGTGGGAGGATGTG
GCACCCACCCTGCCTGACGGTCACTTTGATGGGATCCTGTACGACACGTACCCACTCTCG
GAGGAGACCTGGCACACACACCAGTTCAACTTCATCAAGAACCACGCCTTTCGCCTGCTG
AAGCCGGGGGGCGTCCTCACCTACTGCAACCTCACCTCCTGGGGGGAGCTGATGAAGTCC
AAGTACTCAGACATCACCATCATGTTTGAGGAGACGCAGGTGCCCGCGCTGCTGGAGGCC
GGCTTCCGGAGGGAGAACATCCGTACGGAGGTGATGGCGCTGGTCCCACCGGCCGACTGC
CGCTACTACGCCTTCCCACAGATGATCACGCCCCTGGTGACCAAAGGCTGA

Clone variation with respect to NM_000156.4
>OriGene 5' read for NM_000156 unedited
GGTCACAATTGTAAACGACTCCTATAGGCGGCCGCGCATTCGGCACGAGGCGAGGTCGGG
TCGCCGTCCAGCCTGCAGCATGAGCGCCCCCAGCGCGACCCCCATCTTCGCGCCCGGCGA
GAACTGCAGCCCCGCGTGGGGGGCGGCGCCCGCGGCCTACGACGCAGCGGACACGCACCT
GCGCATCCTGGGCAAGCCGGTGATGGAGCGCTGGGAGACCCCCTATATGCACGCGCTGGC
CGCCGCCGCCTCCTCCAAGGGGGCCGGGTCCTGGAGGTGGGCTTTGGCATGGCCATCGCA
GCGTCAAAGGTGCAGGAGGCGCCCATTGATGAGCATTGGATCATCGAGTGCAATGACGGC
GTCTTCCAGCGGCTCCGGGACTGGGCCCCACGGCAGACACACAAGGTCATCCCCTTGAAA
GGCCTGTGGGAGGATGTGGCACCCACCCTGCCTGACGGTCACTTTGATGGGATCCTGTAC
GACACGTACCCACTCTCGGAGGAGACCTGGCACACACACCAGTTCAACTTCATCAAGAAC
CACGCCTTTCGCCTGCTGAAGCCGGGGGGCGTCCTCACCTACTGCAACCTCACCTNCTGG
GGGGAGCTGATGAAGTCCAAGTACTCAGACATCACCATCATGTTTGAGGAGACGCAGGTG
CCCGCGCTGCTGGAGGCCGCCTTCCGGAGGGAGAACATCCGTACGGAGGTGATGGCGCTG
GTCCCACCGGCCGACTGGCGCTACTACGCCTTTCCACAGATGATCACGCCCCCTGGTGAC
CAAAGGCTGAGCCCCCACCCCGGCCCGGGCACACCCATGCCCTCCTCCGTGCCCTTCCTG
GCCGGAGTCCAAGCTGTCGCACCAGCCCTGGGCTGATCCCAGCTGTGTGTAACAGAAGCT
TTCCCG
>OriGene 3' read for NM_000156 unedited
NCGTGGGGGCGNACCACGCCCTTGTTTACTTCACCTCAGGCACCCTGCAGTGGGCAGCAG
TGACCCTCACATANAAGCTGGGAAAGCTGCTGGTGACACACAGCTGGGATCAGCCCTGGG
CTGGTGGGACCCCTCACAGAGAAGCCGGGAAAGCTTCTGGTGACACACAGCTGGGATCAG
CCCAGGGCTGGTGCGACACCCTGGACTCCCGGCCAGGAATGCACGGAGGAGGGCATGGGT
GTGGCCGGGCCGGGGTGGGGGCTCAGCCTTTGGTCACCAGGGGCGTGATCATCTGTGGGA
AGGCGTAGTAGCGGCAGTCGGCCGGTGGGACCAGCGCCATCACCTCCGTACGGATGTTCT
CCCTCCGGAAGCCGGACTCCAGCAGCGCGGGCACCTGCGTCTCCTCAAACATGATGGTGA
TGCCTGAGTACTTGGACTTCATCAGCTCCCCCCAGCAGGTGAGGTTGCAGTAGGTGAGGA
CGCCCCCCGGCTTCAGCAGGCGAAAGGAGTGGNTCTTGATGAAGTTGAACTGGTGTGTGT
GCCACGTCTCCTCCGAGAGTGGTACGTGTCTTACAGGATTCCATTAAGTGACCGTCAGGC
CAGGTGGGTGTCACATCCTCCCACAAGCCTTTNAAGGGGGTGACCCTTGTGTGCTCGCCG
TGGGGCCCANNCCCGGAACCGCTGTAAGACCCCGGCATTTGCCTCCAAGATCCCATGGTC
ATCAATGGTTGCCTTCTGTTCTCTTTGCCNCTGCGATCGTTCTNTCCAATACCCACCTTC
CNGGNCCCGGCTCCCTTTCAGCAGCACGCTGTGNTNGTGCCATTACGTGCTTAAAAGAGG
GGCGACCAATCTAGACTCCTTCACCCCTCTCTCCCCAGGAAGCCNGAAACGACTGTATCT
CCTCATCCATAATGTCCTCGGGACCGCTGCTCCCCATGAGAGCANCATAATACTGACCGT
CTACGCCCNAG
Restriction Sites NotI-NotI     
ACCN NM_000156
Insert Size 1170 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_000156.4, NP_000147.1
RefSeq Size 1086 bp
RefSeq ORF 711 bp
Locus ID 2593
UniProt ID Q14353
Protein Families Druggable Genome
Protein Pathways Arginine and proline metabolism, Glycine, serine and threonine metabolism, Metabolic pathways
Gene Summary The protein encoded by this gene is a methyltransferase that converts guanidoacetate to creatine, using S-adenosylmethionine as the methyl donor. Defects in this gene have been implicated in neurologic syndromes and muscular hypotonia, probably due to creatine deficiency and accumulation of guanidinoacetate in the brain of affected individuals. Two transcript variants encoding different isoforms have been described for this gene. Pseudogenes of this gene are found on chromosomes 2 and 13. [provided by RefSeq, Feb 2012]
Transcript Variant: This variant (1) is the longer transcript but encodes the shorter isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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