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Glucose 6 phosphate isomerase (GPI) (NM_000175) Human Untagged Clone
CAT＃: SC120063
GPI (untagged)-Human glucose-6-phosphate isomerase (GPI), transcript variant 2
CNY 4,160.00
Product images
CNY 1,999.00
CNY 2,700.00
热销推荐Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | AMF; GNPI; NLK; PGI; PHI; SA-36; SA36 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC120063 sequence for NM_000175 edited (data generated by NextGen Sequencing)
ATGGCCGCTCTCACCCGGGACCCCCAGTTCCAGAAGCTGCAGCAATGGTACCGCGAGCAC CGCTCCGAGCTGAACCTGCGCCGCCTCTTCGATGCCAACAAGGACCGCTTCAACCACTTC AGCTTGACCCTCAACACCAACCATGGGCATATCCTGGTGGATTACTCCAAGAACCTGGTG ACGGAGGACGTGATGCGGATGCTGGTGGACTTGGCCAAGTCCAGGGGCGTGGAGGCCGCC CGGGAGCGGATGTTCAATGGTGAGAAGATCAACTACACCGAGGGTCGAGCCGTGCTGCAC GTGGCTCTGCGGAACCGGTCAAACACACCCATCCTGGTAGACGGCAAGGATGTGATGCCA GAGGTCAACAAGGTTCTGGACAAGATGAAGTCTTTCTGCCAGCGTGTCCGGAGCGGTGAC TGGAAGGGGTACACAGGCAAGACCATCACGGACGTCATCAACATTGGCATTGGCGGCTCC GACCTGGGACCCCTCATGGTGACTGAAGCCCTTAAGCCATACTCTTCAGGAGGTCCCCGC GTCTGGTATGTCTCCAACATTGATGGAACTCACATTGCCAAAACCCTGGCCCAGCTGAAC CCCGAGTCCTCCCTGTTCATCATTGCCTCCAAGACCTTTACTACCCAGGAGACCATCACG AATGCAGAGACGGCGAAGGAGTGGTTTCTCCAGGCGGCCAAGGATCCTTCTGCAGTGGCG AAGCACTTTGTTGCCCTGTCTACTAACACAACCAAAGTGAAGGAGTTTGGAATTGACCCT CAAAACATGTTCGAGTTCTGGGATTGGGTGGGAGGACGCTACTCGCTGTGGTCGGCCATC GGACTCTCCATTGCCCTGCACGTGGGTTTTGACAACTTCGAGCAGCTGCTCTCGGGGGCT CACTGGATGGACCAGCACTTCCGCACGACGCCCCTGGAGAAGAACGCCCCCGTCTTGCTG GCCCTGCTGGGTATCTGGTACATCAACTGCTTTGGGTGTGAGACACACGCCATGCTGCCC TATGACCAGTACCTGCACCGCTTTGCTGCGTACTTCCAGCAGGGCGACATGGAGTCCAAT GGGAAATACATCACCAAATCTGGAACCCGTGTGGACCACCAGACAGGCCCCATTGTGTGG GGGGAGCCAGGGACCAATGGCCAGCATGCTTTTTACCAGCTCATCCACCAAGGCACCAAG ATGATACCCTGTGACTTCCTCATCCCGGTCCAGACCCAGCACCCCATACGGAAGGGTCTG CATCACAAGATCCTCCTGGCCAACTTCTTGGCCCAGACAGAGGCCCTGATGAGGGGAAAA TCGACGGAGGAGGCCCGAAAGGAGCTCCAGGCTGCGGGCAAGAGTCCAGAGGACCTTGAG AGGCTGCTGCCACATAAGGTCTTTGAAGGAAATCGCCCAACCAACTCTATTGTGTTCACC AAGCTCACACCATTCATGCTTGGAGCCTTGGTCGCCATGTATGAGCACAAGATCTTCGTT CAGGGCATCATCTGGGACATCAACAGCTTTGACCAGTGGGGAGTGGAGCTGGGAAAGCAG CTGGCTAAGAAAATAGAGCCTGAGCTTGATGGCAGTGCTCAAGTGACCTCTCACGACGCT TCTACCAATGGGCTCATCAACTTCATCAAGCAGCAGCGCGAGGCCAGAGTCCAATAA Clone variation with respect to NM_000175.3 >OriGene 5' read for NM_000175 unedited
TTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGTAGTCCCGCCATGGC CGCTCTCACCCGGGACCCCCAGTTCCAGAAGCTGCAGCAATGGTACCGCGAGCACCGCTC CGAGCTGAACCTGCGCCGCCTCTTCGATGCCAACAAGGACCGCTTCAACCACTTCAGCTT GACCCTCAACACCAACCATGGGCATATCCTGGTGGATTACTCCAAGAACCTGGTGACGGA GGACGTGATGCGGATGCTGGTGGACTTGGCCAAGTCCAGGGGCGTGGAGGCCGCCCGGGA GCGGATGTTCAATGGTGAGAAGATCAACTACACCGAGGGTCGAGCCGTGCTGCACGTGGC TCTGCGGAACCGGTCAAACACACCCATCCTGGTAGACGGCAAGGATGTGATGCCAGAGGT CAACAAGGTTCTGGACAAGATGAAGTCTTTCTGCCAGCGTGTCCGGAGCGGTGACTGGAA GGGGTACACAGGCAAGACCATCACGGACGTCATCAACATTGGCATTGGCGGCTCCGACCT GNGACCCCTCATGGTGACTGAAGCCCTTAAGCCATACTCTTCAGGAGGTCCCCGCGTCTG GTATGTCTCCAACATTGATGGAACTCACATTGCCAAAACCCTGGCCCAGCTGAACCCCGA GTCCTCCCTGTTCATCATTGCCTNCAAGACCTTTACTACCCCAGAGACCATCACGAATGC AAAGACGGCGAAGGAGTGGTTTCTCCAGGCGGGCAAAGATCCTTCTGCAGTGGCGAAACA CTTTGTTGCCCTGTCTACTAACCCACCAAAGTGAAGAGTTTGGGATTGACCCTCAAACAT GTTCCAGTTCTGGGATTGGTGGGGAGACGCTACTCCTGTGTCCGGCATCGGACTTTCATT GCCCTGCACGGGGTTTTGAACACTT >OriGene 3' read for NM_000175 unedited
TTACCGCGGCCGCATCTANATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTGCAAAAGAACACCAGTTTATTGGACTCTGGCCTCGCGCTGCTGCTTGATGAAA TTGATGAACCCATTGGTAAAAACGTCGTGAGAGGGCACTTGAGCACTGCCATCAAGCTCA GGCTTTATTTTCTTAACCAGCTGGTTTCCCAGCTCCACTCCCCACTGGTCAAAGCTGGTG ATGTCCCAAATGATGCCCTGAACGAAAATCTTGGGCTCATACATGGGGACCAAGGGTCCA AACATGAATGGGGTGAGCTTGGGGAACACAATAAAGTTGGGTGGGCGATTTCCTTCAAAG ACCTTATGTGGCAGCAGCCTCTCAAGGGCCTCTGGACTCTTGCCCGCAACCTGGAGCTCC TTTCGGGCCTCCTCCGGCGATTTTCCCCTCATCAAGGCCTCTGTCTGGGCCAAAAAATTG GCCAGGAGGATCTTGTGATGCAAACCCTTCCCTATGGGGGGCTGGGTCTGGACCGGGATG AGGAAATCACAGGGTATCATCTTGGTGCCTTGGTGGATGAGCTGGTAAAAAACATGCTGG CCATTGGTCCCTGGCTCCCCCCCACAATGGGGCCTGTCTGGTGGGCCCACCGGTTCCAAA ATTGGTGATGTTTTTCCCATTGGACTCCATGGCGCCCTGGTGGAAGTACCCAACAAAACG GGGCAGGTCTGGTCATAGGGCACCATGGGGTGGGCTCACACCCAAGCAGTTGATGTCCAA ATCCCCACAGGGCCACAAGACGGGGGCCTTTTTTTCAGGGGCGTTTTGCGGAAATGCTGG TCCTCACATGGAGCCCCCCAGAACACTTGTTTAAAATTGTCAAAACCCCCTCCCGGGGCA TGGAAAAGCCCTAGGCCACCCCACGAAA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_000175 |
| Insert Size | 1750 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000175.2, NP_000166.2 |
| RefSeq Size | 2075 bp |
| RefSeq ORF | 1677 bp |
| Locus ID | 2821 |
| UniProt ID | P06744 |
| Domains | PGI |
| Protein Families | Druggable Genome |
| Protein Pathways | Amino sugar and nucleotide sugar metabolism, Glycolysis / Gluconeogenesis, Metabolic pathways, Pentose phosphate pathway, Starch and sucrose metabolism |
| Gene Summary | This gene encodes a member of the glucose phosphate isomerase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In the cytoplasm, the gene product functions as a glycolytic enzyme (glucose-6-phosphate isomerase) that interconverts glucose-6-phosphate and fructose-6-phosphate. Extracellularly, the encoded protein (also referred to as neuroleukin) functions as a neurotrophic factor that promotes survival of skeletal motor neurons and sensory neurons, and as a lymphokine that induces immunoglobulin secretion. The encoded protein is also referred to as autocrine motility factor based on an additional function as a tumor-secreted cytokine and angiogenic factor. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2016] Transcript Variant: This variant (2) differs in the 5' UTR and uses a downstream start codon, compared to variant 3. The encoded isoform (2) has a shorter N-terminus, compared to isoform 3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201232 | GPI (Myc-DDK-tagged)-Human glucose-6-phosphate isomerase (GPI), transcript variant 2 |
CNY 4,152.00 |
|
| RC201232L1 | Lenti ORF clone of Human glucose-6-phosphate isomerase (GPI), transcript variant 2, Myc-DDK-tagged |
CNY 6,552.00 |
|
| RC201232L2 | Lenti ORF clone of Human glucose-6-phosphate isomerase (GPI), transcript variant 2, mGFP tagged |
CNY 6,460.00 |
|
| RC201232L3 | Lenti ORF clone of Human glucose-6-phosphate isomerase (GPI), transcript variant 2, Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC201232L4 | Lenti ORF clone of Human glucose-6-phosphate isomerase (GPI), transcript variant 2, mGFP tagged |
CNY 6,460.00 |
|
| RG201232 | GPI (tGFP-tagged) - Human glucose-6-phosphate isomerase (GPI), transcript variant 2 |
CNY 5,040.00 |
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