HMGCL (NM_000191) Human Untagged Clone
CAT#: SC120045
HMGCL (untagged)-Human 3-hydroxymethyl-3-methylglutaryl-CoA lyase (HMGCL), nuclear gene encoding mitochondrial protein, transcript variant 1
CNY 2,400.00
CNY 2,950.00
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Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | HL |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC120045 sequence for NM_000191 edited (data generated by NextGen Sequencing)
ATGGCAGCAATGAGGAAGGCGCTTCCGCGGCGACTGGTGGGCTTGGCGTCCCTCCGGGCT GTCAGCACCTCATCTATGGGCACTTTACCAAAGCGGGTGAAAATTGTGGAAGTTGGTCCC CGAGATGGACTACAAAATGAAAAGAATATCGTATCTACTCCAGTGAAAATCAAGCTGATA GACATGCTTTCTGAAGCAGGACTCTCTGTTATAGAAACCACCAGCTTTGTGTCTCCTAAG TGGGTTCCCCAGATGGGTGACCACACTGAAGTCTTGAAGGGCATTCAGAAGTTTCCTGGC ATCAACTACCCAGTCCTGACCCCAAATTTGAAAGGCTTCGAGGCAGCGGTTGCTGCTGGA GCCAAGGAAGTAGTCATCTTTGGAGCTGCCTCAGAGCTCTTCACCAAGAAGAACATCAAT TGTTCCATAGAGGAGAGTTTTCAGAGGTTTGACGCAATCCTGAAGGCAGCGCAGTCAGCC AATATTTCTGTGCGGGGGTACGTCTCCTGTGCTCTTGGCTGCCCTTATGAAGGGAAGATC TCCCCAGCTAAAGTAGCTGAGGTCACCAAGAAGTTCTACTCAATGGGCTGCTACGAGATC TCCCTGGGGGACACCATTGGTGTGGGCACCCCAGGGATCATGAAAGACATGCTGTCTGCT GTCATGCAGGAAGTGCCTCTGGCTGCCCTGGCTGTCCACTGCCATGACACCTATGGTCAA GCCCTGGCCAACACCTTGATGGCCCTGCAGATGGGAGTGAGTGTCGTGGACTCTTCTGTG GCAGGACTTGGAGGCTGTCCCTACGCACAGGGGGCATCAGGAAACTTGGCCACAGAAGAC CTGGTCTACATGCTAGAGGGCTTGGGCATTCACACGGGTGTGAATCTCCAGAAGCTTCTG GAAGCTGGAAACTTTATCTGTCAAGCCCTGAACAGAAAAACTAGCTCCAAAGTGGCTCAG GCTACCTGTAAACTCTGA Clone variation with respect to NM_000191.2 654 a=>g >OriGene 5' read for NM_000191 unedited
NNNNNGGTCGACATTTGTATACGACTCACTATAGGCGGCCGCGNAATTCGCACGAGGTGG CAGCAATGAGGAAGGCGCTTCCGCGGCGACTGGTGGGCTTGGCGTCCCTCCGGGCTGTCA GCACCTCATCTATGGGCACTTTACCAAAGCGGGTGAAAATTGTGGAAGTTGGTCCCCGAG ATGGACTACAAAATGAAAAGAATATCGTATCTACTCCAGTGAAAATCAAGCTGATAGACA TGCTTTCTGAAGCAGGACTCTCTGTTATAGAAACCACCAGCTTTGTGTCTCCTAAGTGGG TTCCCCAGATGGGTGACCACACTGAAGTCTTGAAGGGCATTCAGAAGTTTCCTGGCATCA ACTACCCAGTCCTGACCCCAAATTTGAAAGGCTTCGAGGCAGCGGTTGCTGCTGGAGCCA AGGAAGTAGTCATCTTTGGAGCTGCCTCAGAGCTCTTCACCAAGAAGAACATCAATTGTT CCATAGAGGAGAGTTTTCAGAGGTTTGACGCAATCCTGAAGGCAGCGCAGTCAGCCAATA TTTCTGTGCGGGGGTACGTCTCCTGTGCTCTTGGCTGCCCTTATGAAGGGAAGATCTCCC CAGCTAAAGTAGCTGAGGTCACCAAGAAGTTCTACTCAATGGGCTGCTACGAGATCTCCC TGNGGGACACCATTGGTGTGGGCACCCCAGGGATCATGANAGACATGCTGTCTGCTGTCA TGCANGAAGTGCCTCTGGCTGNCCTGGCTGTCCACTGCCATGACACCTATGGTCAAGCCC CTGGCCAACACCTTGATGGCCCTGCAAATGGGAGTGAGTGTCGTGGACTCTTCTGTGGCA GGACTTGGAGGCTGTCCCTACCACAGGGGGCAT >OriGene 3' read for NM_000191 unedited
CACTGGTACCGCGCCGCATTCTANGTCGAGTTTATTTCTTTTTTTTTTATGATGTGCCAT TCAACCTTTAATTACATAAGCTGTTTTCAAAAATCACATTCAGCGTGGAGATTTTCCAGA GATGCTGGAGTTGGCAGATGGCCACCACGTAGCTCTCCACTTTCCACAAATGGCCTCTGC CAGCTTGGGAAGAACCTCCATGACCTGTGTCCTGTTAAGTCAGAGAGCAAGGGCCCCACG GCCATGGCAGGGTGGAGCCGTGTAGAGGGTGGCCAGGTGGCCAGCTCGCGGATTCCATCC AGAGAGGAGACTCAAGGATCATGCTAAGTAGGGAACGGGGTTCCCACACGTCCTCAGGCA TTCAACTCCTGGGCCAGGGTCCGTAACAAAGGGAGACTTCAGCCCTCAAAGCCTCTCACT CCTCAGGTCTGGGCAGGAGGTCCTTCTGCCAAGCAGCTCCTCCTGCCCTTCGCCTGCTTT CTGCCAGGAGAGACCTCTGTGTAGAGCTGGCTATGAGGTACCTGCACCATTTAACCTATT CTCATTTCCATGTCCCCATCCATGAATCATCTGTGTGTGCCCCTATTTCCACATCATCCC CAGGGCTTCAGGTGGGCAAGGGGCTCAGAGTTTACAGGTAGCCTGAGCCACTTTGGAGCT AGTTTTTCTGTTCAGGGCTTGACAGATAAAGTTTCCAGCTTCCAGAAGCTTCTGGAGATT CACACCCGTGTGAATGCCCAAGCCCTCTAGCATGTAGACCAGGTCTTCTGTGGCCAGTTT TCTGATGCCNCCTGTGCGTANGGACAGCCCNCCAGTCCTGCCCCAGAAGAGTCACGACAC TCACTCCCATCTGCNAGCCCATCAGGTGGTTGCCAGGGCTTGACCTTAGTGTCATGGCAG TGNAAGNCCGGGCACCCGAAGCACTCCCTGCTGAACGCNAACGCTGTT |
Restriction Sites | NotI-NotI |
ACCN | NM_000191 |
Insert Size | 1750 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_000191.2, NP_000182.2 |
RefSeq Size | 1617 bp |
RefSeq ORF | 978 bp |
Locus ID | 3155 |
UniProt ID | P35914 |
Domains | HMGL-like |
Protein Families | Druggable Genome |
Protein Pathways | Butanoate metabolism, Metabolic pathways, Synthesis and degradation of ketone bodies, Valine, leucine and isoleucine degradation |
Gene Summary | The protein encoded by this gene belongs to the HMG-CoA lyase family. It is a mitochondrial enzyme that catalyzes the final step of leucine degradation and plays a key role in ketone body formation. Mutations in this gene are associated with HMG-CoA lyase deficiency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009] Transcript Variant: This variant (1) encodes the longer isoform (1). |
Documents
Product Manuals |
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC203817 | HMGCL (Myc-DDK-tagged)-Human 3-hydroxymethyl-3-methylglutaryl-CoA lyase (HMGCL), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 2,400.00 |
|
RC203817L3 | Lenti ORF clone of Human 3-hydroxymethyl-3-methylglutaryl-CoA lyase (HMGCL), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC203817L4 | Lenti ORF clone of Human 3-hydroxymethyl-3-methylglutaryl-CoA lyase (HMGCL), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
RG203817 | HMGCL (tGFP-tagged) - Human 3-hydroxymethyl-3-methylglutaryl-CoA lyase (HMGCL), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 4,000.00 |
|
SC322255 | HMGCL (untagged)-Human 3-hydroxymethyl-3-methylglutaryl-CoA lyase (HMGCL), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 2,400.00 |