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PAH (NM_000277) Human Untagged Clone
CAT＃: SC120014
PAH (untagged)-Human phenylalanine hydroxylase (PAH)
CNY 3,656.00
Product images
CNY 1,999.00
CNY 3,600.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | PH; PKU; PKU1 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC120014 sequence for NM_000277 edited (data generated by NextGen Sequencing)
ATGTCCACTGCGGTCCTGGAAAACCCAGGCTTGGGCAGGAAACTCTCTGACTTTGGACAG GAAACAAGCTATATTGAAGACAACTGCAATCAAAATGGTGCCATATCACTGATCTTCTCA CTCAAAGAAGAAGTTGGTGCATTGGCCAAAGTATTGCGCTTATTTGAGGAGAATGATGTA AACCTGACCCACATTGAATCTAGACCTTCTCGTTTAAAGAAAGATGAGTATGAATTTTTC ACCCATTTGGATAAACGTAGCCTGCCTGCTCTGACAAACATCATCAAGATCTTGAGGCAT GACATTGGTGCCACTGTCCATGAGCTTTCACGAGATAAGAAGAAAGACACAGTGCCCTGG TTCCCAAGAACCATTCAAGAGCTGGACAGATTTGCCAATCAGATTCTCAGCTATGGAGCG GAACTGGATGCTGACCACCCTGGTTTTAAAGATCCTGTGTACCGTGCAAGACGGAAGCAG TTTGCTGACATTGCCTACAACTACCGCCATGGGCAGCCCATCCCTCGAGTGGAATACATG GAGGAAGAAAAGAAAACATGGGGCACAGTGTTCAAGACTCTGAAGTCCTTGTATAAAACC CATGCTTGCTATGAGTACAATCACATTTTTCCACTTCTTGAAAAGTACTGTGGCTTCCAT GAAGATAACATTCCCCAGCTGGAAGACGTTTCTCAGTTCCTGCAGACTTGCACTGGTTTC CGCCTCCGACCTGTAGCTGGCCTGCTTTCCTCTCGGGATTTCTTGGGTGGCCTGGCCTTC CGAGTCTTCCACTGCACACAGTACATCAGACATGGATCCAAGCCCATGTATACCCCCGAA CCTGACATCTGCCATGAGCTGTTGGGACATGTGCCCTTGTTTTCAGATCGCAGCTTTGCC CAGTTTTCCCAGGAAATTGGCCTTGCCTCTCTGGGTGCACCTGATGAATACATTGAAAAG CTCGCCACAATTTACTGGTTTACTGTGGAGTTTGGGCTCTGCAAACAAGGAGACTCCATA AAGGCATATGGTGCTGGGCTCCTGTCATCCTTTGGTGAATTACAGTACTGCTTATCAGAG AAGCCAAAGCTTCTCCCCCTGGAGCTGGAGAAGACAGCCATCCAAAATTACACTGTCACG GAGTTCCAGCCCCTGTATTACGTGGCAGAGAGTTTTAATGATGCCAAGGAGAAAGTAAGG AACTTTGCTGCCACAATACCTCGGCCCTTCTCAGTTCGCTACGACCCATACACCCAAAGG ATTGAGGTCTTGGACAATACCCAGCAGCTTAAGATTTTGGCTGATTCCATTAACAGTGAA ATTGGAATCCTTTGCAGTGCCCTCCAGAAAATAAAGTAA Clone variation with respect to NM_000277.1 696 a=>g;735 g=>a >OriGene 5' read for NM_000277 unedited
NNNNGGGGGGGNNNNNNNNNNCNNNCGGNNCGGTACAAAATTGTATACGACTCATATAGG CGGCCGCGAAATTCGCACGAGGTACCTGAGGCCCTAAAAACCAGAGACCTCACTCCCGGG GAGCCAGCATGTCCACTGCGGNNTCCTGGAAAACCCAGGCTTGGGCAGGAAACTCTCTGA CTTTGGACAGGAAACAAGCTATATTGAAGACAACTGCAATCAAAATGGTGCCATATCACT GATCTTCTCACTCAAAGAAGAAGTTGGTGCATTGGCCAAAGTATTGCGCTTATTTGAGGA GAATGATGTAAACCTGACCCACATTGAATCTAGACCTTCTCGTTTAAAGAAAGATGAGTA TGAATTTTTCACCCATTTGGATAAACGTAGCCTGCCTGCTCTGACAAACATCATCAAGAT CTTGAGGCATGACATTGGTGCCACTGTCCATGAGCTTTCACGAGATAAGAAGAAAGACAC AGTGCCCTGGTTCCCAAGAACCATTCAAGAGCTGGACAGATTTGCCAATCAGATTCTCAG CTATGGAGCGGAACTGGATGCTGACCACCCTGGTTTTAAAGATCCTGTGTACCGTGCAAG ACGGAAGCAGTTTGCTGACATTGCCTACAACTACCGCCATGGGCAGCCCATCCCTCGAGT GGAATACATGGAGGAAGAAAAGAAAACATGGNGCACAGTGTTCAAGACTCTGAAGTCCTT GTATAAAACCCATGCTTGCTATGAGTACAATCACATTTTTCCACTTCTTGAAAAGTACTG TGGCTTCCATGAAGATAACATTCCCCAGCTGGAAGACGTTTCTCAGTTTCTGCAGACTTG CACTGGGTTCCGCCTCCGACCTGTAGCTGGCCTGCTTTCCCTCTCGGAATTCTGGGTGGC CTGGCCTTNCCGAGTCTTCACTGCCACAGTACATCAGACATGG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_000277 |
| Insert Size | 2300 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000277.1, NP_000268.1 |
| RefSeq Size | 2680 bp |
| RefSeq ORF | 1359 bp |
| Locus ID | 5053 |
| UniProt ID | P00439 |
| Domains | biopterin_H, ACT |
| Protein Families | Druggable Genome |
| Protein Pathways | Metabolic pathways, Phenylalanine, tyrosine and tryptophan biosynthesis, Phenylalanine metabolism |
| Gene Summary | This gene encodes a member of the biopterin-dependent aromatic amino acid hydroxylase protein family. The encoded phenylalanine hydroxylase enzyme hydroxylates phenylalanine to tyrosine and is the rate-limiting step in phenylalanine catabolism. Deficiency of this enzyme activity results in the autosomal recessive disorder phenylketonuria. [provided by RefSeq, Aug 2017] Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204694 | PAH (Myc-DDK-tagged)-Human phenylalanine hydroxylase (PAH) |
CNY 3,656.00 |
|
| RC204694L3 | Lenti ORF clone of Human phenylalanine hydroxylase (PAH), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC204694L4 | Lenti ORF clone of Human phenylalanine hydroxylase (PAH), mGFP tagged |
CNY 6,056.00 |
|
| RG204694 | PAH (tGFP-tagged) - Human phenylalanine hydroxylase (PAH) |
CNY 5,256.00 |
