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Caspase 8 (CASP8) (NM_033358) Human Untagged Clone
CAT＃: SC119997
CASP8 (untagged)-Human caspase 8, apoptosis-related cysteine peptidase (CASP8), transcript variant E
CNY 3,990.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ALPS2B; CAP4; Casp-8; FLICE; MACH; MCH5 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_033358 edited
GAATTCGGCACGAGGGATAGGCCTGTGACGAAGGTGCTACCATCGTGAGAGTAAGATTAT ATTCTCCTGCCTTTTAAAAAGATGGACTTCAGCAGAAATCTTTATGATATTGGGGAACAA CTGGACAGTGAAGATCTGGCCTCCCTCAAGTTCCTGAGCCTGGACTACATTCCGCAAAGG AAGCAAGAACCCATCAAGGATGCCTTGATGTTATTCCAGAGACTCCAGGAAAAGAGAATG TTGGAGGAAAGCAATCTGTCCTTCCTGAAGGAGCTGCTCTTCCGAATTAATAGACTGGAT TTGCTGATTACCTACCTAAACACTAGAAAGGAGGAGATGGAAAGGGAACTTCAGACACCA GGCAGGGCTCAAATTTCTGCCTACAGGGTCATGCTCTATCAGATTTCAGAAGAAGTGAGC AGATCAGAATTGAGGTCTTTTAAGTTTCTTTTGCAAGAGGAAATCTCCAAATGCAAACTG GATGATGACATGAACCTGCTGGATATTTTCATAGAGATGGAGAAGAGGGTCATCCTGGGA GAAGGAAAGTTGGACATCCTGAAAAGAGTCTGTGCCCAAATCAACAAGAGCCTGCTGAAG ATAATCAACGACTATGAAGAATTCAGCAAAGAGAGAAGCAGCAGCCTTGAAGGAAGTCCT GATGAATTTTCAAATGGGGAGGAGTTGTGTGGGGTAATGACAATCTCGGACTCTCCAAGA GAACAGGATAGTGAATCACAGACTTTGGACAAAGTTTACCAAATGAAAAGCAAACCTCGG GGATACTGTCTGATCATCAACAATCACAATTTTGCAAAAGCACGGGAGAAAGTGCCCAAA CTTCACAGCATTAGGGACAGGAATGGAACACACTTGGATGCAGGGGCTTTGACCACGACC TTTGAAGAGCTTCATTTTGAGATCAAGCCCCACGATGACTGCACAGTAGAGCAAATCTAT GAGATTTTGAAAATCTACCAACTCATGGACCACAGTAACATGGACTGCTTCATCTGCTGT ATCCTCTCCCATGGAGACAAGGGCATCATCTATGGCACTGATGGACAGGAGGCCCCCATC TATGAGCTGACATCTCAGTTCACTGGTTTGAAGTGCCCTTCCCTTGCTGGAAAACCCAAA GTGTTTTTTATTCAGGCTTGTCAGGGGGATAACTACCAGAAAGGTATACCTGTTGAGACT GATTCAGAGGAGCAACCCTATTTAGAAATGGATTTATCATCACCTCAAACGAGATATATC CCGGATGAGGCTGACTTTCTGCTGGGGATGGCCACTGTGAATAACTGTGTTTCCTACCGA AACCCTGCAGAGGGAACCTGGTACATCCAGTCACTTTGCCAGAGCCTGAGAGAGCGATGT CCTCGAGGCGATGATATTCTCACCATCCTGACTGAAGTGAACTATGAAGTAAGCAACAAG GATGACAAGAAAAACATGGGGAAACAGATGCCTCAGCCTACTTTCACACTAAGAAAAAAA CTTGTCTTCCCTTCTGATTGATGGTGCTATTTTGTTTGTTTTGTTTTGTTTTGTTTTTTT GAGACAGAATCTCGCTCTGTCGCCCAGGCTGGAGTGCAGTGGCGTGATCTCGGCTCACCG CAAGCTCCGCCTCCCGGGTTCACGCCATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGAC TACAGGGGCCCGCCACCACACCTGGCTAATTTTTTAAAAATATTTTTAGTAGAGACAGGG TTTCACTGTGTTAGCCAGGGTGGTCTTGATCTCCTGACCTCGTGATCCACCCACCTCGGC CTCCCAAAGTGCTGGGATTACAGGCGTGAGCCACCGCGCCTGGCCGATGGTACTATTTAG ATATAACACTATGTTTATTTACTAATTTTCTAGATTTTCTACTTTATTAATTGTTTTGCA CTTTTTTATAAGAGCTAAAGTTAAATAGGATATTAACAACAATAACACTGTCTCCTTTCT CTTATGCTTAAGGCTTTGGGAATGTTTTTAGCTGGTGGCAATAAATACCAGACACGTACA AAATCCAGCTATGAATATAGAGGGCTTATGATTCAGATTGTTATCTATCAACTATAAGCC CACTGTTAATATTCTATTAACTTTAATTCTCTTTCAAAGCTAAATTCCACACTACCACAT TAAAAAAATTAGAAAGTAGCCACGTATGGTGGCTCATGTCTATAATCCCAGCACTTTGGG AGGTTGAGGTGGGAGGATTGCTTGAACCCAAGAGGTCAAGGCTGCAGTGAGCCATGTTCA CACCGCTGCACTCAAGCTTGGGTGACAGAGCAAGACCCCGTCTCAAAAAAAATTTTTTTT TTAATAAAACAAAATTTGTTTGAAAACTTTTAAAAATTCAAATGATTTTTACAAGTTTTA AATAAGCTCTCCCCAAACTTGCTTTATGCCTTCTTATTGCTTTTATGATATATATATGCT TGGCTAACTATATTTGCTTTTTGCTAACAATGCTCTGGGGTCTTTTTATGCATTTGCATT TGCTCTTTCATCTCTGCTTGGATTATTTTAAATCATTAGGAATTAAGTTATCTTTAAAAT TTAAGTATCTTTTTTCAAAAACATTTTTTAATAGAATAAAATATAATTTGATCTTAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AACTCGAC >OriGene 5' read for NM_033358 unedited
TCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAAATTCGCACGAGGGATAGGC CTGTGACGAAGGTGCTACCATCGTGAGAGTAAGATTATATTCTCCTGCCTTTTAAAAAGA TGGACTTCAGCAGAAATCTTTATGATATTGGGGAACAACTGGACAGTGAAGATCTGGCCT CCCTCAAGTTCCTGAGCCTGGACTACATTCCGCAAAGGAAGCAAGAACCCATCAAGGATG CCTTGATGTTATTCCAGAGACTCCAGGAAAAGAGAATGTTGGAGGAAAGCAATCTGTCCT TCCTGAAGGAGCTGCTCTTCCGAATTAATAGACTGGATTTGCTGATTACCTACCTAAACA CTAGAAAGGAGGAGATGGAAAGGGAACTTCAGACACCAGGCAGGGCTCAAATTTCTGCCT ACAGGGTCATGCTCTATCAGATTTCAGAAGAAGTGAGCAGATCAGAATTGAGGTCTTTTA AGTTTCTTTTGCAAGAGGAAATCTCCAAATGCAAACTGGATGATGACATGAACCTGCTGG ATATTTTCATAGAGATGGAGAAGAGGGTCATCCTGNGAGAAGGAAAGTTGGACATCCTGA AAAGAGTCTGTGCCCAAATCAACAAGAGCCTGCTGAAGATAATCAACGACTATGAAGAAT TCAGCAAAGAGAGAAGCAGCAGCCTTGAAGGAAGTCCTGATGAATTTTCAAATGGGGAGG AGTTGTGTGGGGTAATGACAATCTCGGACTCTTCAAGAGAACAGGATAGTGAATCACAGA CTTTGGACANNAGTTACCAAATGAAAAGCANACCTCGNGATACTGTCTGATCATAACATC ACATTNTGCAAAGCACGGAGAAATGCCCAACTCACGCATAGGACAGGATGGACAACTNGA TGCAGGCTTGCCCGACTTGAGAGCTATTTGAATAGCCCACATACTGACATNACAATTTGA A |
| Restriction Sites | NotI-NotI |
| ACCN | NM_033358 |
| Insert Size | 2900 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_033358.1, NP_203522.1 |
| RefSeq Size | 1088 bp |
| RefSeq ORF | 708 bp |
| Locus ID | 841 |
| UniProt ID | Q14790 |
| Protein Families | Druggable Genome, Protease |
| Protein Pathways | Alzheimer's disease, Apoptosis, Huntington's disease, NOD-like receptor signaling pathway, p53 signaling pathway, Pathways in cancer, RIG-I-like receptor signaling pathway, Toll-like receptor signaling pathway, Viral myocarditis |
| Gene Summary | This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (E), also known as Beta-1, has multiple differences, one of which causes a frameshift, compared to variant G. It encodes isoform E, which is shorter than isoform G. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217090 | CASP8 (Myc-DDK-tagged)-Human caspase 8, apoptosis-related cysteine peptidase (CASP8), transcript variant E |
CNY 3,990.00 |
|
| RC217090L3 | Lenti-ORF clone of CASP8 (Myc-DDK-tagged)-Human caspase 8, apoptosis-related cysteine peptidase (CASP8), transcript variant E |
CNY 5,890.00 |
|
| RC217090L4 | Lenti-ORF clone of CASP8 (mGFP-tagged)-Human caspase 8, apoptosis-related cysteine peptidase (CASP8), transcript variant E |
CNY 5,890.00 |
|
| RG217090 | CASP8 (tGFP-tagged) - Human caspase 8, apoptosis-related cysteine peptidase (CASP8), transcript variant E |
CNY 4,240.00 |
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