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PEPD (NM_000285) Human Untagged Clone
CAT＃: SC119982
PEPD (untagged)-Human peptidase D (PEPD), transcript variant 1
CNY 3,656.00
| Cited in 3 publications. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | PROLIDASE |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_000285, the custom clone sequence may differ by one or more nucleotides
ATGGCGGCGGCCACCGGACCCTCGTTTTGGCTGGGGAATGAAACCCTGAAGGTGCCGCTGGCGCTCTTTG CCTTGAACCGGCAGCGCCTGTGTGAGCGGCTGCGGAAGAACCCTGCTGTGCAGGCCGGCTCCATCGTGGT CCTGCAGGGCGGGGAGGAGACTCAGCGCTACTGCACCGACACCGGGGTCCTCTTCCGCCAGGAGTCCTTC TTTCACTGGGCGTTCGGTGTCACTGAGCCAGGCTGCTATGGTGTCATCGATGTTGACACTGGGAAGTCGA CCCTGTTTGTGCCCAGGCTTCCTGCCAGCCATGCCACCTGGATGGGAAAGATCCATTCCAAGGAGCACTT CAAGGAGAAGTATGCCGTGGACGACGTCCAGTACGTAGATGAGATTGCCAGCGTCCTGACGTCACAGAAG CCCTCTGTCCTCCTCACTTTGCGTGGCGTCAACACGGACAGCGGCAGTGTCTGCAGGGAGGCCTCCTTTG ACGGCATCAGCAAGTTCGAAGTCAACAATACCATTCTTCACCCAGAGATCGTTGAGTGCCGAGTGTTTAA GACGGATATGGAGCTGGAGGTTCTGCGCTATACCAATAAAATCTCCAGCGAGGCCCACCGTGAGGTAATG AAGGCTGTAAAAGTGGGAATGAAAGAATATGAGTTGGAAAGCCTCTTCGAGCACTACTGCTACTCCCGGG GCGGCATGCGCCACAGCTCCTACACCTGCATCTGCGGCAGTGGTGAGAACTCAGCCGTGCTACACTACGG ACACGCCGGAGCTCCCAACGACCGAACGATCCAGAATGGGGATATGTGCCTGTTCGACATGGGCGGTGAG TATTACTGCTTCGCTTCCGACATCACCTGCTCCTTTCCCGCCAACGGCAAGTTCACTGCAGACCAGAAGG CCGTCTATGAGGCAGTGCTGCGGAGCTCCCGTGCCGTCATGGGTGCCATGAAGCCAGGTGTCTGGTGGCC TGACATGCACCGCCTGGCTGACCGCATCCACCTGGAGGAGCTGGCCCACATGGGCATCCTGAGCGGCAGC GTGGACGCCATGGTCCAGGCTCACCTGGGGGCCGTGTTTATGCCTCACGGGCTTGGCCACTTCCTGGGCA TTGACGTGCACGACGTGGGAGGCTACCCAGAGGGCGTGGAGCGCATCGACGAGCCCGGCCTGCGGAGCCT GCGCACTGCACGGCACCTGCAGCCAGGCATGGTGCTCACCGTGGAGCCGGGCATCTACTTCATCGACCAC CTCCTGGATGAGGCCCTGGCGGACCCGGCCCGCGCCTCCTTCCTTAACCGCGAGGTCCTGCAGCGCTTTC GCGGTTTTGGCGGGGTCCGCATCGAGGAGGACGTCGTGGTGACTGACAGCGGCATAGAGCTGCTGACCTG CGTGCCCCGCACTGTGGAAGAGATTGAAGCATGCATGGCAGGCTGTGACAAGGCCTTTACCCCCTTCTCT GGCCCCAAGTAG >OriGene 5' read for NM_000285 unedited
ATTTTTCANATTTTGTATACGACTCACTTATAGGGCGGCCGCGATTCGGCACGAGGGTGA CGCCGGTGCCGGGCGAACTGGCGGCGGCCACCGGACCCTCGTTTTGGCTGGGGAATGAAA CCCTGAAGGTGCCGCTGGCGCTCTTTGCCTTGAACCGGCAGCGCCTGTGTGAGCGGCTGC GGAAGAACCCTGCTGTGCAGGCCGGCTCCATCGTGGTCCTGCAGGGCGGGGAGGAGACTC AGCGCTACTGCACCGACACCGGGGTCCTCTTCCGCCAGGAGTCCTTCTTTCACTGGGCGT TCGGTGTCACTGAGCCAGGCTGCTATGGTGTCATCGATGTTGACACTGGGAAGTCGACCC TGTTTGTGCCCAGGCTTCCTGCCAGCCATGCCACCTGGATGGGAAAGATCCATTCCAAGG AGCACTTCAAGGAGAAGTATGCCGTGGACGACGTCCAGTACGTAGATGAGATTGCCAGCG TCCTGACGTCACAGAAGCCCTCTGTCCTCCTCACTTTGCGTGGCGTCAACACGGACAGCG GCAGTGTCTGCAGGGAGGCCTCCTTTGACGGCATCAGCAAGTTCGAAGTCAACAATACCA TTCTTCACCCAGAGATCGTTGAGTGCCGAGTGTTTAAGACGGATATGGAGCTGGAGGTTC TGCGCTATACCAATAAAATCTCCAGCGAGGCCCACCGTGAGGTAATGAAGGCTGTAAAAG TGGGAATGAAAGAATATGAGTTGGAAAGCCTCTTCGAGCACTACTGCTACTCCNGGNGCG GCATGCGCCACAGCTCCTACACCTGCATCTGCGGCAGTGGTGAGACNTCAGCCGTGCTAC ACTACGGACACGCCGGAGCTNCCAACGACCGAACGATTCAGAATGGGGATATGTGCCCTG TTCGACATG >OriGene 3' read for NM_000285 unedited
TTGGACCGCGCCGCATTCTANATCGAGTTTTTTTTTTTTTTTTTTGACCATTTTAGGAAA CCTTTTATTGCAAATGCCATTCTGCATATTGATTTTTGACAGAAAGTATCAGAAATGCTT CTTTCCTGGGAAAAGGAATATAAATGACAGCAAGACACATTTTAGTTGCTACTAAAGAAC AGCATTATTTTCAATCATTTTAAGTCGCTCATTTAATAAGCAAGGTATAAAACAGATTAA AGGTGGGAGCCTGCAAAAGGGTAATTAAAAAAGTGTTTCCTCCCCGGGAAACAGCACTGT TTGGTCTGATCAAATGCCGAAGCTGGGATCTGATTCTGGGTGCCGTCTCTCGCTACTGGA GTGCTGACCAGCAGGCTGCCCATCACGAAAAGAGGTTGCAAGGCCAGGCCCCCAGGTGCG CTGGGATTTCTGGCTGGCTCTACTTGGGGCCAGAGAAGGGGGTAAAGGCCTTGTCACAGC CTGCCATGCATGCTTCAATCTCTTCCACAGTGCGGGGCACGCAGGTCAGCAGCTCTATGC CGCTGTCAGTCACCACGACGTCCTCCTCGATGCGGACCCCGCCAAAACCGCGAAAGCGCT GCAGGACCTCGCGGTTAAGGAAGGAGGCGCGGGCCGGGTCCGCCAGGGCCTCATCCAGGA GGTGGTCGATGAAGTAGATGCCCGGCTCCACAGTGAGCACCATGCCTGGCTGCANGTGCC GTGCANTGCGCANGCTCCGCAGGCCCGGCTCGTCGATGCGCTCCACGCCCTCTGGGTAGC CTCCCCCTCATGCCCGTAATGCCAGGAAGTGGCCAACCCGTGAAGCATAAACCCGCGCCC AGGGAACCCTGACCCTGGCGTTCACCCTGCCTCAAGATGGCCATGTGGGCCAACTCCTTC AAGGGGAGCGGTAANCCAGCCGGGCTGTAAGGCCCCCAACCCTGGCTTTTGCCCCCCTGA CGGCCGGAGCTCG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_000285 |
| Insert Size | 1910 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000285.1, NP_000276.1 |
| RefSeq Size | 1888 bp |
| RefSeq ORF | 1482 bp |
| Locus ID | 5184 |
| UniProt ID | P12955 |
| Domains | Peptidase_M24, AMP_N |
| Protein Families | Druggable Genome, Protease |
| Gene Summary | This gene encodes a member of the peptidase family. The protein forms a homodimer that hydrolyzes dipeptides or tripeptides with C-terminal proline or hydroxyproline residues. The enzyme serves an important role in the recycling of proline, and may be rate limiting for the production of collagen. Mutations in this gene result in prolidase deficiency, which is characterized by the excretion of large amount of di- and tri-peptides containing proline. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2009] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). |
Citations (3)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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Loss of peptidase D binding restores the tumor suppressor functions of oncogenic p53 mutants
,null,
Communications Biology
,PubMed ID 34880421
[PEPD]
|
|
PEPD is a pivotal regulator of p53 tumor suppressor
,Yang, L;Li, Y;Bhattacharya, A;Zhang, Y;,
Nat Commun
,PubMed ID 29233996
[PEPD]
|
|
Prolidase Directly Binds and Activates Epidermal Growth Factor Receptor and Stimulates Downstream Signaling
,Lu Yang, Yun Li, Yi Ding, Kyoung-Soo Choi, A. Latif Kazim, and Yuesheng Zhang,
J. Biol. Chem., Jan 2013; 288: 2365 - 2375.
[PEPD]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201970 | PEPD (Myc-DDK-tagged)-Human peptidase D (PEPD), transcript variant 1 |
CNY 3,656.00 |
|
| RC201970L3 | Lenti ORF clone of Human peptidase D (PEPD), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201970L4 | Lenti ORF clone of Human peptidase D (PEPD), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG201970 | PEPD (tGFP-tagged) - Human peptidase D (PEPD), transcript variant 1 |
CNY 5,256.00 |
|
| SC322376 | PEPD (untagged)-Human peptidase D (PEPD), transcript variant 1 |
CNY 3,656.00 |
