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Rb (RB1) (NM_000321) Human Untagged Clone
CAT＃: SC119971
RB1 (untagged)-Human retinoblastoma 1 (RB1)
CNY 10,208.00
货期*
现货
规格
| Cited in 5 publications. |
Product images
经常一起买 (3)
RB1 mouse monoclonal antibody,clone OTI13A6
CNY 1,999.00
CNY 2,700.00
热销推荐Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | OSRC; p105-Rb; p110-RB1; pp110; PPP1R130; pRb; RB |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_000321 edited
GGGGAGGGCGCGTCCGGTTTTTCTCAGGGGACGTTGAAATTATTTTTGTAACGGGAGTCG GGAGAGGACGGGGCGTGCCCCGACGTGCGCGCGCGTCGTCCTCCCCGGCGCTCCTCCACA GCTCGCTGGCTCCCGCCGCGGAAAGGCGTCATGCCGCCCAAAACCCCCCGAAAAACGGCC GCCACCGCCGCCGCTGCCGCCGCGGAACCCCCGGCACCGCCGCCGCCGCCCCCTCCTGAG GAGGACCCAGAGCAGGACAGCGGCCCGGAGGACCTGCCTCTCGTCAGGCTTGAGTTTGAA GAAACAGAAGAACCTGATTTTACTGCATTATGTCAGAAATTAAAGATACCAGATCATGTC AGAGAGAGAGCTTGGTTAACTTGGGAGAAAGTTTCATCTGTGGATGGAGTATTGGGAGGT TATATTCAAAAGAAAAAGGAACTGTGGGGAATCTGTATCTTTATTGCAGCAGTTGACCTA GATGAGATGTCGTTCACTTTTACTGAGCTACAGAAAAACATAGAAATCAGTGTCCATAAA TTCTTTAACTTACTAAAAGAAATTGATACCAGTACCAAAGTTGATAATGCTATGTCAAGA CTGTTGAAGAAGTATGATGTATTGTTTGCACTCTTCAGCAAATTGGAAAGGACATGTGAA CTTATATATTTGACACAACCCAGCAGTTCGATATCTACTGAAATAAATTCTGCATTGGTG CTAAAAGTTTCTTGGATCACATTTTTATTAGCTAAAGGGGAAGTATTACAAATGGAAGAT GATCTGGTGATTTCATTTCAGTTAATGCTATGTGTCCTTGACTATTTTATTAAACTCTCA CCTCCCATGTTGCTCAAAGAACCATATAAAACAGCTGTTATACCCATTAATGGTTCACCT CGAACACCCAGGCGAGGTCAGAACAGGAGTGCACGGATAGCAAAACAACTAGAAAATGAT ACAAGAATTATTGAAGTTCTCTGTAAAGAACATGAATGTAATATAGATGAGGTGAAAAAT GTTTATTTCAAAAATTTTATACCTTTTATGAATTCTCTTGGACTTGTAACATCTAATGGA CTTCCAGAGGTTGAAAATCTTTCTAAACGATACGAAGAAATTTATCTTAAAAATAAAGAT CTAGATGCAAGATTATTTTTGGATCATGATAAAACTCTTCAGACTGATTCTATAGACAGT TTTGAAACACAGAGAACACCACGAAAAAGTAACCTTGATGAAGAGGTGAATGTAATTCCT CCACACACTCCAGTTAGGACTGTTATGAACACTATCCAACAATTAATGATGATTTTAAAT TCAGCAAGTGATCAACCTTCAGAAAATCTGATTTCCTATTTTAACAACTGCACAGTGAAT CCAAAAGAAAGTATACTGAAAAGAGTGAAGGATATAGGATACATCTTTAAAGAGAAATTT GCTAAAGCTGTGGGACAGGGTTGTGTCGAAATTGGATCACAGCGATACAAACTTGGAGTT CGCTTGTATTACCGAGTAATGGAATCCATGCTTAAATCAGAAGAAGAACGATTATCCATT CAAAATTTTAGCAAACTTCTGAATGACAACATTTTTCATATGTCTTTATTGGCGTGCGCT CTTGAGGTTGTAATGGCCACATATAGCAGAAGTACATCTCAGAATCTTGATTCTGGAACA GATTTGTCTTTCCCATGGATTCTGAATGTGCTTAATTTAAAAGCCTTTGATTTTTACAAA GTGATCGAAAGTTTTATCAAAGCAGAAGGCAACTTGACAAGAGAAATGATAAAACATTTA GAACGATGTGAACATCGAATCATGGAATCCCTTGCATGGCTCTCAGATTCACCTTTATTT GATCTTATTAAACAATCAAAGGACCGAGAAGGACCAACTGATCACCTTGAATCTGCTTGT CCTCTTAATCTTCCTCTCCAGAATAATCACACTGCAGCAGATATGTATCTTTCTCCTGTA AGATCTCCAAAGAAAAAAGGTTCAACTACGCGTGTAAATTCTACTGCAAATGCAGAGACA CAAGCAACCTCAGCCTTCCAGACCCAGAAGCCATTGAAATCTACCTCTCTTTCACTGTTT TATAAAAAAGTGTATCGGCTAGCCTATCTCCGGCTAAATACACTTTGTGAACGCCTTCTG TCTGAGCACCCAGAATTAGAACATATCATCTGGACCCTTTTCCAGCACACCCTGCAGAAT GAGTATGAACTCATGAGAGACAGGCATTTGGACCAAATTATGATGTGTTCCATGTATGGC ATATGCAAAGTGAAGAATATAGACCTTAAATTCAAAATCATTGTAACAGCATACAAGGAT CTTCCTCATGCTGTTCAGGAGACATTCAAACGTGTTTTGATCAAAGAAGAGGAGTATGAT TCTATTATAGTATTCTATAACTCGGTCTTCATGCAGAGACTGAAAACAAATATTTTGCAG TATGCTTCCACCAGGCCCCCTACCTTGTCACCAATACCTCACATTCCTCGAAGCCCTTAC AAGTTTCCTAGTTCACCCTTACGGATTCCTGGAGGGAACATCTATATTTCACCCCTGAAG AGTCCATATAAAATTTCAGAAGGTCTGCCAACACCAACAAAAATGACTCCAAGATCAAGA ATCTTAGTATCAATTGGTGAATCATTCGGGACTTCTGAGAAGTTCCAGAAAATAAATCAG ATGGTATGTAACAGCGACCGTGTGCTCAAAAGAAGTGCTGAAGGAAGCAACCCTCCTAAA CCACTGAAAAAACTACGCTTTGATATTGAAGGATCAGATGAAGCAGATGGAAGTAAACAT CTCCCAGGAGAGTCCAAATTTCAGCAGAAACTGGCAGAAATGACTTCTACTCGAACACGA ATGCAAAAGCAGAAAATGAATGATAGCATGGATACCTCAAACAAGGAAGAGAAATGAGGA TCTCAGGACCTTGGTGGACACTGTGTACACCTCTGGATTCATTGTCTCTCACAGATGTGA CTGTATAACTTTCCCAGGTTCTGTTTATGGCCACATTTAATATCTTCAGCTCTTTTTGTG GATATAAAATGTGCAGATGCAATTGTTTGGGTGATTCCTAAGCCACTTGAAATGTTAGTC ATTGTTATTTATACAAGATTGAAAATCTTGTGTAAATCCTGCCATTTAAAAAGTTGTAGC AGATTGTTTCCTCTTCCAAAGTAAAATTGCTGTGCTTTATGGATAGTAAGAATGGCCCTA GAGTGGGAGTCCTGATAACCCAGGCCTGTCTGACTACTTTGCCTTCTTTTGTAGCATATA GGTGATGTTTGCTCTTGT >OriGene 5' read for NM_000321 unedited
NNNNGGTTCACATTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGCGGGA GAGGACGGGGCGTGCCCCGACGTGCGCGCGCGTCGTCCTCCCCGGCGCTCCTCCACAGCT CGCTGGCTCCCGCCGCGGAAAGGCGTCATGCCGCCCAAAACCCCCCGAAAAACGGCCGCC ACCGCCGCCGCTGCCGCCGCGGAACCCCCGGCACCGCCGCCGCCGCCCCCTCCTGAGGAG GACCCAGAGCAGGACAGCGGCCCGGAGGACCTGCCTCTCGTCAGGCTTGAGTTTGAAGAA ACAGAAGAACCTGATTTTACTGCATTATGTCAGAAATTAAAGATACCAGATCATGTCAGA GAGAGAGCTTGGTTAACTTGGGAGAAAGTTTCATCTGTGGATGGAGTATTGGGAGGTTAT ATTCAAAAGAAAAAGGAACTGTGGGGAATCTGTATCTTTATTGCAGCAGTTGACCTAGAT GAGATGTCGTTCACTTTTACTGAGCTACAGAAAAACATAGAAATCAGTGTCCATAAATTC TTTAACTTACTAAAAGAAATTGATACCAGTACCAAAGTTGATAATGCTATGTCAAGACTG TTGAAGAAGTATGATGTATTGTTTGCACTCTTCAGCAAATTGGAAAGGACATGTGAACTT ATATATTTGACACAACCCAGCAGTTCGATATCTACTGAAATAAATTCTGCATTGGTGCTA AAAGTTTCTTGGATCACATTTTTATTAGCTAAAGGGGAAGTATTACAAATGGAAGATGAT CTGGTGATTTCATTTCAGTTAATGCTATGTGTCCTTGACTATTTTATTAAACTCTCACCT CCCATGTTGCTCAAAGAACCATATAAAACAGCTGTTATACCCATT >OriGene 3' read for NM_000321 unedited
NNNGGTATTTCTTTGGACCGCGGNNCCGCATTTNANGATCGAGTTTTTTTTTTTTTTTTT TTTTTTTTTAAAACTCTTAACAACTAATTTATTTTAAAATAGACAAACTATTAATTGTAG AAAATAGTAACATAGCAATTTTAAATGTACAGTTTTAACCAATACAAAAAAGCAATAAAG CAATAATATCTGAAGCATTATTTAAGAAATCTCAATACACGATCTCTGAAGTTCCTAAAA TTCTGGCACTAATTCTAATGGGAACTTAGTAGCAAAAGACCCAGAAATAGTAAGCCCTTG ACCTAAAAACTAACTGATTTGTATGATATTCATGCAGAAACAATGATGAAATGGAGTCAA GTTTTCTAGTGTCATTGTTATCAAAATAACTGTCAAAATAGTAAGTTTGAAACTTAAATG AGCACAAAATAAAATTTTGTTTTCTAACAAGACCAGATTTCTTTTTAAAAATAATTCTGA GTTAGACAAAGTGATTTTCCTAAAAGCTAGCTGAAGCTACCTTAAATATCCCCTATTTTA AGTTACAGCATCTCTAAATAAGTTAATCACACAAGATAGTTTAAATACACCTTTAGGTGT AGGGGAGGGGAGAAGCGCCTCTTTTTCTAATGCAGCTGTTTTAATTTGAAGCTTTTGCAC AAAATCAGATAGAAACATTAATGCCTAACCCATAATGACCCTTGATTACTTGTAATTTTG GACTAGAAAAATGTGGCTTTGAACATGCCAGTGTTAGACCATACTGACTTAAAAAATAAA AGATAAATAGCCATTTGCCAGTTCAAAAGATCATGGAAGAATGTACCATTCCAGAAAAAC ACANACCTTTCCAAATTAAGGAGGCAGAATAATTTGAGATGCATTAGACAAACTCTCAGA AAA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_000321 |
| Insert Size | 4600 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000321.1, NP_000312.1 |
| RefSeq Size | 4740 bp |
| RefSeq ORF | 2787 bp |
| Locus ID | 5925 |
| UniProt ID | P06400 |
| Domains | RB_B, RB_A, CYCLIN |
| Protein Families | Druggable Genome, Transcription Factors |
| Protein Pathways | Bladder cancer, Cell cycle, Chronic myeloid leukemia, Glioma, Melanoma, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Prostate cancer, Small cell lung cancer |
| Gene Summary | The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq, Jul 2008] |
Citations (5)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Viral and tumor antigen-specific CD8 T-cell responses in Merkel cell carcinoma
,Samimi, M;Benlalam, H;Aumond, P;Gaboriaud, P;Fradin, D;Kervarrec, T;Florenceau, L;Vignard, V;Blom, A;Touzé, A;Gervois, N;Labarriere, N;,
Cell. Immunol.
,PubMed ID 31472938
[RB1]
|
|
A Point Mutation in DNA Polymerase ß (POLB) Gene Is Associated with Increased Progesterone Receptor (PR) Expression and Intraperitoneal Metastasis in Gastric Cancer
,Tan, X;Wu, X;Ren, S;Wang, H;Li, Z;Alshenawy, W;Li, W;Cui, J;Luo, G;Siegel, RS;Fu, SW;Lu, Y;,
J Cancer
,PubMed ID 27471563
[RB1]
|
|
IRF8 suppresses pathological cardiac remodelling by inhibiting calcineurin signalling
,Jiang, DS;Wei, X;Zhang, XF;Liu, Y;Zhang, Y;Chen, K;Gao, L;Zhou, H;Zhu, XH;Liu, PP;Bond Lau, W;Ma, X;Zou, Y;Zhang, XD;Fan, GC;Li, H;,
Nat Commun, Feb 2014.
,PubMed ID 24526256
[RB1]
|
|
Cyclin-dependent kinase inhibitor 2B regulates efferocytosis and atherosclerosis
,Kojima, Y;Downing, K;Kundu, R;Miller, C;Dewey, F;Lancero, H;Raaz, U;Perisic, L;Hedin, U;Schadt, E;Maegdefessel, L;Quertermous, T;Leeper, NJ;,
J. Clin. Invest., Feb 2014.
,PubMed ID 24531546
[RB1]
|
|
Phosphatidylinositol 3-Kinase/Protein Kinase C-Induced Phosphorylation of Sp1 and p107 Repressor Release Have a Critical Role in Histone Deacetylase Inhibitor-Mediated Depression of Transcription of the Luteinizing Hormone Receptor Gene
,Ying Zhang, Mingjuan Liao, and Maria L. Dufau,
Mol. Cell. Biol.,Sep 2006; 26: 6748 - 6761.
[RB1]
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| RG206933 | RB1 (tGFP-tagged) - Human retinoblastoma 1 (RB1) |
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