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p21 (CDKN1A) (NM_000389) Human Untagged Clone
CAT＃: SC119947
CDKN1A (untagged)-Human cyclin-dependent kinase inhibitor 1A (p21, Cip1) (CDKN1A), transcript variant 1
CNY 1,800.00
| Cited in 7 publications. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CAP20; CDKN1; CIP1; MDA-6; P21; p21CIP1; SDI1; WAF1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_000389 edited
ATGTCAGAACCGGCTGGGGATGTCCGTCAGAACCCATGCGGCAGCAAGGCCTGCCGCCGC CTCTTCGGCCCAGTGGACAGCGAGCAGCTGAGCCGCGACTGTGATGCGCTAATGGCGGGC TGCATCCAGGAGGCCCGTGAGCGATGGAACTTCGACTTTGTCACCGAGACACCACTGGAG GGTGACTTCGCCTGGGAGCGTGTGCGGGGCCTTGGCCTGCCCAAGCTCTACCTTCCCACG GGGCCCCGGCGAGGCCGGGATGAGTTGGGAGGAGGCAGGCGGCCTGGCACCTCACCTGCT CTGCTGCAGGGGACAGCAGAGGAAGACCATGTGGACCTGTCACTGTCTTGTACCCTTGTG CCTCGCTCAGGGGAGCAGGCTGAAGGGTCCCCAGGTGGACCTGGAGACTCTCAGGGTCGA AAACGGCGGCAGACCAGCATGACAGATTTCTACCACTCCAAACGCCGGCTGATCTTCTCC AAGAGGAAGCCCTAA >OriGene 5' read for NM_000389 unedited
TGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGCCGAAGTCAGTTCC TTGTGGAGCCGGAGCTGGGCGCGGATTCGCCGAGGCACCGAGGCACTCAGAGGAGGCGCC ATGTCAGAACCGGCTGGGGATGTCCGTCAGAACCCATGCGGCAGCAAGGCCTGCCGCCGC CTCTTCGGCCCAGTGGACAGCGAGCAGCTGAGCCGCGACTGTGATGCGCTAATGGCGGGC TGCATCCAGGAGGCCCGTGAGCGATGGAACTTCGACTTTGTCACCGAGACACCACTGGAG GGTGACTTCGCCTGGGAGCGTGTGCGGGGCCTTGGCCTGCCCAAGCTCTACCTTCCCACG GGGCCCCGGCGAGGCCGGGATGAGTTGGGAGGAGGCAGGCGGCCTGGCACCTCACCTGCT CTGCTGCAGGGGACAGCAGAGGAAGACCATGTGGACCTGTCACTGTCTTGTACCCTTGTG CCTCGCTCAGGGGAGCAGGCTGAAGGGTCCCCAGGTGGACCTGGAGACTCTCAGGGTCGA AAACGGCGGCAGACCAGCATGACAGATTTCTACCACTCCAAACGCCGGCTGATCTTCTCC AAGAGGAAGCCCTAATCCGCCCACAGGAAGCCTGCAGTCCTGGAAGCGCGAGGGCCTCAA AGGCCCGCTCTACATCTTCTGCCTTAGTCTCAGTTTGTGTGTCTTAATTATTATTTGTGT TTTAATTTAAACACCTCCTCATGTACATACCCTGGCCGCCCCCTGCCCCCCAGCCTCTGG CATTAGAATTATNTAAACAANAACTANGCGGTTGAATGAGAAGTTCCTAAGAGTGCNTGG CATTNTTATTTTATGAAATACTATTTAAAGCCTNCTCANCCCGTGNTCTCCTTTCCTCTC TCCCGGAGGTGGGGTGGGCCGGCTCATGCCAGCTACTTNCTCCTC >OriGene 3' read for NM_000389 unedited
CGCGGCACGCTATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT TTAAAGGCACTAAAAATCATTTATTGAGCACCTGCTGTATATTCAGCATTGGGGGAGGAG CTGTGAAAGACACAAAACAGTACAGGGTGGGGTCCCTGCCCTCGAGAGGTTTACAGTCTA GGGGGAGAAACGGGAACCAGGACACATGGGGAGCCGAGAGAAAACAGTCCAGGCCAGTAT GTTACAGGAGCTGGAAGGTGTTTGGGGTCAAACCCCAATACTCCAAGTACACTAAGCACT TCAGTGCCTCCAGGGGCTCAACGTTAGTGCCAGGAAAGACAACTACTCCCAGCCCCATAT GAGCCCACGTGGCATGCCCTGTCCATAGCCTTTACTGCCACCATCTTAAAATGTCTGACT CCTTGTTCCGCTGCTAATCAAAGTGCAATGAACTGGGGAGGGATGGGGTGGATGAAGAAG GTCGCTGGACAATTTGAGGGGCCAGTGTCTCCCTCCTAAAAAGATCTACTCCCCCATCAT ATACCCCTAACACAGAGATAACCCCACTCAAGGGGGCCTGTGCCACCACATGGGACCCTC ACCCCCACAGTTAGAGGAGGGGGCGGCCGCTGCTTGAGCTGCCTGAGGTAAAACTAGGGT GCCCTTCTTCTTGTGTGTCCCTTCCCCTTCAGTCCATTGAGCTGGGGGTGGGGGTGGGAC AGGCACCTCAGAGCCATCTGGAGCTGATAGGGTCCTGAAGGGAAACGACCAGGGGGAGGA ACAGCACCAAAGGGGATTTGCCNAGCCCATCTGGAAAATATGTTTACCTGGGCCTCCTCA AAAGGTCAGGGGAGCCAAAAAGGAAATAGCTAACACTGAGACGCCTCNCCAGTGCAGGTA AAAGGCCCATGAGGCAGCGGGGTGGTTTGTTCAGGACCCACCTTCCCCTGCCTTAAAGAA CAAGGAG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_000389 |
| Insert Size | 2100 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000389.2, NP_000380.1 |
| RefSeq Size | 2140 bp |
| RefSeq ORF | 495 bp |
| Locus ID | 1026 |
| UniProt ID | P38936 |
| Domains | CDI |
| Protein Families | Druggable Genome |
| Protein Pathways | Bladder cancer, Cell cycle, Chronic myeloid leukemia, ErbB signaling pathway, Glioma, Melanoma, p53 signaling pathway, Pathways in cancer, Prostate cancer |
| Gene Summary | This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or -cyclin-dependent kinase4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen, a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of cyclin-dependent kinase2, and may be instrumental in the execution of apoptosis following caspase activation. Mice that lack this gene have the ability to regenerate damaged or missing tissue. Multiple alternatively spliced variants have been found for this gene. [provided by RefSeq, Sep 2015] Transcript Variant: This variant (1) differs in the 5' UTR, lacks an in-frame portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 3. The resulting isoform (1) has a shorter N-terminus, compared to isoform 2. Variants 1, 2, 4 and 5 encode the same isoform 1. |
Citations (7)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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AUF1 ligand circPCNX reduces cell proliferation by competing with p21 mRNA to increase p21 production
,Tsitsipatis, D;Grammatikakis, I;Driscoll, RK;Yang, X;Abdelmohsen, K;Harris, SC;Yang, JH;Herman, AB;Chang, MW;Munk, R;Martindale, JL;Mazan-Mamczarz, K;De, S;Lal, A;Gorospe, M;,
Nucleic acids research
,PubMed ID 33444453
[CDKN1A]
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Apolipoprotein E2 modulates cell cycle function to promote proliferation in pancreatic cancer cells via regulation of the c-Myc-p21Waf1 signalling pathway
,Du, S;Wang, H;Cai, J;Ren, R;Zhang, W;Wei, W;Shen, X;,
Biochem. Cell Biol.
,PubMed ID 32167787
[CDKN1A]
|
|
Androgen Receptor-Mediated Growth Suppression of HPr-1AR and PC3-Lenti-AR Prostate Epithelial Cells
,Kim, YC;Chen, C;Bolton, EC;,
PLoS ONE
,PubMed ID 26372468
[CDKN1A]
|
|
The Effects of Two Polymorphisms on p21cip1 Function and Their Association with Alzheimer's Disease in a Population of European Descent
,Yates, SC;Zafar, A;Rabai, EM;Foxall, JB;Nagy, S;Morrison, KE;Clarke, C;Esiri, MM;Christie, S;Smith, AD;Nagy, Z;,
PLoS ONE
,PubMed ID 25625488
[CDKN1A]
|
|
Inactivation of the mTORC1-Eukaryotic Translation Initiation Factor 4E Pathway Alters Stress Granule Formation
,Marie-Josée Fournier, Laetitia Coudert, Samia Mellaoui, Pauline Adjibade, Cristina Gareau, Marie-France Côté, Nahum Sonenberg, René C. Gaudreault, and Rachid Mazroui,
Mol. Cell. Biol., Jun 2013; 33: 2285 - 2301.
,PubMed ID 23547259
[CDKN1A]
|
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Depletion of the poly(C) binding proteins CP1 and 2 from K562 cells leads to p53-independent induction of cyclin dependent kinase inhibitor (CDKN1A) and G1 arrest
,Shelly A. Waggoner, Gregg J. Johannes, and Stephen A. Liebhaber,
J. Biol. Chem., Feb 2009; 10.1074/jbc.M806986200.
[CDKN1A]
|
|
The Aurora Kinase Inhibitor VX-680 Induces Endoreduplication and Apoptosis Preferentially in Cells with Compromised p53-Dependent Postmitotic Checkpoint Function
,Farid Gizatullin, Yao Yao, Victor Kung, Matthew W. Harding, Massimo Loda, and Geoffrey I. Shapiro,
Cancer Res., Aug 2006; 66: 7668 - 7677.
[CDKN1A]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201817 | CDKN1A (Myc-DDK-tagged)-Human cyclin-dependent kinase inhibitor 1A (p21, Cip1) (CDKN1A), transcript variant 1 |
CNY 1,800.00 |
|
| RC201817L1 | Lenti ORF clone of Human cyclin-dependent kinase inhibitor 1A (p21, Cip1) (CDKN1A), transcript variant 1, Myc-DDK-tagged |
CNY 4,200.00 |
|
| RC201817L2 | Lenti ORF clone of Human cyclin-dependent kinase inhibitor 1A (p21, Cip1) (CDKN1A), transcript variant 1, mGFP tagged |
CNY 4,200.00 |
|
| RC201817L3 | Lenti ORF clone of Human cyclin-dependent kinase inhibitor 1A (p21, Cip1) (CDKN1A), transcript variant 1, Myc-DDK-tagged |
CNY 4,200.00 |
|
| RC201817L4 | Lenti ORF clone of Human cyclin-dependent kinase inhibitor 1A (p21, Cip1) (CDKN1A), transcript variant 1, mGFP tagged |
CNY 4,200.00 |
|
| RG201817 | CDKN1A (tGFP-tagged) - Human cyclin-dependent kinase inhibitor 1A (p21, Cip1) (CDKN1A), transcript variant 1 |
CNY 3,400.00 |
