ARSA (NM_000487) Human Untagged Clone

CAT#: SC119855

ARSA (untagged)-Human arylsulfatase A (ARSA), transcript variant 1



  "NM_000487" in other vectors (7)

CNY 3,784.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
Rabbit Polyclonal Anti-ARSA Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms ASA; MLD
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC119855 sequence for NM_000487 edited (data generated by NextGen Sequencing)
ATGTCCATGGGGGCACCGCGGTCCCTCCTCCTGGCCCTGGCTGCTGGCCTGGCCGTTGCC
CGTCCGCCCAACATCGTGCTGATCTTTGCCGACGACCTCGGCTATGGGGACCTGGGCTGC
TATGGGCACCCCAGCTCTACCACTCCCAACCTGGACCAGCTGGCGGCGGGAGGGCTGCGG
TTCACAGACTTCTACGTGCCTGTGTCTCTGTGCACACCCTCTAGGGCCGCCCTCCTGACC
GGCCGGCTCCCGGTTCGGATGGGCATGTACCCTGGCGTCCTGGTGCCCAGCTCCCGGGGG
GGCCTGCCCCTGGAGGAGGTGACCGTGGCCGAAGTCCTGGCTGCCCGAGGCTACCTCACA
GGAATGGCCGGCAAGTGGCACCTTGGGGTGGGGCCTGAGGGGGCCTTCCTGCCCCCCCAT
CAGGGCTTCCATCGATTTCTAGGCATCCCGTACTCCCACGACCAGGGCCCCTGCCAGAAC
CTGACCTGCTTCCCGCCGGCCACTCCTTGCGACGGTGGCTGTGACCAGGGCCTGGTCCCC
ATCCCACTGTTGGCCAACCTGTCCGTGGAGGCGCAGCCCCCCTGKCTGCCCGGACTAGAG
GCCCGCTACATGGCTTTCGCCCATGACCTCATGGCCGACGCCCAGCGCCAGGATCGCCCC
TTCTTCCTGTACTATGCCTCTCACCACACCCACTACCCTCAGTTCAGTGGGCAGAGCTTT
GCAGAGCGTTCAGGCCGCGGGCCATTTGGGGACTCCCTGATGGAGCTGGATGCAGCTGTG
GGGACCCTGATGACAGCCATAGGGGACCTGGGGCTGCTTGAAGAGACGCTGGTCATCTTC
ACTGCAGACAATGGACCTGAGACCATGCGTATGTCCCGAGGCGGCTGCTCCGGTCTCTTG
CGGTGTGGAAAGGGAACGACCTACGAGGGCGGTGTCCGAGAGCCTGCCTTGGCCTTCTGG
CCAGGTCATATCGCTCCCGGCGTGACCCACGAGCTGGCCAGCTCCCTGGACCTGCTGCCT
ACCCTGGCAGCCCTGGCTGGGGCCCCACTGCCCARTGTCACCTTGGATGGCTTTGACCTC
AGCCCCCTGCTGCTGGGCACAGGCAAGAGCCCTCGGCAGTCTCTCTTCTTCTACCCGTCC
TACCCAGACGAGGTCCGTGGGGTTTTTGCTGTGCGGAGTGGAAAGTACAAGGCTCACTTC
TTCACCCAGGGCTCTGCCCACAGTGATACCACTGCAGACCCTGCCTGCCACGCCTCCAGC
TCTCTGACTGCTCATGAGCCCCCGCTGCTCTATGACCTGTCCAAGGACCCTGGTGAGAAC
TACAACCTGCTGGGGGGTGTGGCCGGGGCCACCCCAGAGGTGCTGCAAGCCCTGAAACAG
CTTCAGCTGCTCAAGGCCCAGTTAGACGCAGCTGTGACCTTCGGCCCCAGCCAGGTGGCC
CGGGGCGAGGACCCCGCCCTGCAGATCTGCTGTCATCCTGGCTGCACCCCCCRCCCAGCT
TGCTGCCATTGCCCAGATCCCCATGCCTGA

Clone variation with respect to NM_000487.5
585 g=>k;1055 a=>r;1178 c=>g;1493 g=>r
>OriGene 5' read for NM_000487 unedited
ACCTCTGGATTTGTATCCGACTTCTATAGGCGGCCGCGCATTCGGCACGAGGCCGGCCCG
ACTCCGCTGGTCAGCGCCAAGTGACTTACGCCCCCGACCCTGAGCCCGGACCGCTAGGCG
AGGAGGATCAGATCTCCGCTCGAGAATCTGAAGGTGCCCTGGTCCTGGAGGAGTTCCGTC
CCAGCCCGCGGTCTCCCGGTACTGTCGGGCCCCGGCCCTCTGGAGCTTCAGGAGGCGGCC
GTCAGGGTCGGGGAGTATTTGGGTCCGGGGTCTCAGGGAAGGGCGGCGCCTGGGTCTGCG
GTATCGGAAAGAGCCTGCTGGAGCCAAGTAGCCCTCCCTCTCTTGGGACAGACCCCTCGG
TCCCATGTCCATGGGGGCACCGCGGTCCCTCCTCCTGGCCCTGGCTGCTGGCCTGGCCGT
TGCCCGTCCGCCCAACATCGTGCTGATCTTTGCCGACGACCTCGGCTATGGGGACCTGGG
CTGCTATGGGCACCCCAGCTCTACCACTCCCAACCTGGACCAGCTGGCGGCGGGAGGGCT
GCGGTTCACAGACTTCTACGTGCCTGTGTCTCTGTGCACACCCTCTAGGGCCGCCCTCCT
GACCGGCCGGCTCCCGGTTCGGATGGGCATGTACCCTGGCGTCCTGGTGCCCAGCTCCCG
GGGGGGCCTGCCCCTGGAGGAGGTGACCGTGGCCGAAGTCCTGGCTGCCCGAGGCTACCT
CACAGGAATGGCCGGCAAGTGGCACCTTAGGGTGGGGCCTGAGGGGGCCTTCCTGCCCCC
CATCAAGGCTTCCATCGATTTCTAGGCATCCCGTACTCCCACGACCAGAGCCCCTGCCAG
AACCTGACCTGCTTCCNGNCGGCCACTCCTTGCGACGGTGG
>OriGene 3' read for NM_000487 unedited
TAGAGTCGAGTTTTTTTTTTTTTTTTTTGCAAGTCTCCACTGGTGTTATTACGTTATCAG
GCACAAACCCCCTCCAGACACCTGAGCCTCCCCCACAGGCTCCCAGTGAGGAGCCATCAC
ATGCCCAGGCCAGCCGAGGGGCCCTCAGGCATGGGGATCTGGGCAATGGCAGCAAGCTGG
GCGGGGGGTGCAGCCAGGATGACAGCAGATCTGCAGGGCGGGGTCCTCGCCCCGGGCCAC
CTGGCTGGGGCCGAAGGTCACAGCTGCGTCTAACTGGGCCTTGAGCAGCTGAAGCTGTTT
CAGGGCTTGCAGCACCTCTGGGGTGGCCCCGGCCACACCCCCCAGCAGGTTGTAGTTCTC
ACCAGGGTCCTTGGACAGGTCATAGAGCAGCGGGGGCTCATGAGCAGTCACAGAGCTGGA
GGCGTGGCANGCAGGTTCTGCAGTGGTATCACTGTGGGCAAAGCCCTGGGTGAAGAATGA
GCCCTGTACTTCCCCTTCCCAAGCAAAACCCCACGACCCTCCCCTGGGAAGACGGGATTA
AAAAGGAGACTGGCCAGGGCTCTTGCCTGGCCCACAGCAAGGGCTGATGCAAACCCTTCA
AGGGACATTGGCACGGGGGCCCAACAGGGTTGCACGTAAGCAAAGGTCAGGGACCGCCCA
CTCTTGGGACCGCCCGAACCAAATAACTGCCCCAAGGCCAGCAGTCTCTGCCCCCCCCTG
AGGGCGTCCCTTCCACCCCCATAAACCGAACACCCCCTGGAACACCCGGGCCAAGTCATT
GCCGCATAAAATCCCCGTCTCTTAAACACCCAGCCCCCTGCGTGCCAGGGCCCCCACTGC
CCCTCCTTGGAGCACCAAGGTCCCGCCCACCTCTTAAACTTCTCACAACTAAGGATCGGG
TTGCGATACTCATCCAAATAGGCATTTCGCTTGTCCCCCCTGCCTATACCCCCCATCCCC
TCCTCAN
Restriction Sites NotI-NotI     
ACCN NM_000487
Insert Size 2190 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_000487.3, NP_000478.2
RefSeq Size 2039 bp
RefSeq ORF 1524 bp
Locus ID 410
UniProt ID P15289
Domains Sulfatase
Protein Families Druggable Genome
Protein Pathways Lysosome, Sphingolipid metabolism
Gene Summary The protein encoded by this gene hydrolyzes cerebroside sulfate to cerebroside and sulfate. Defects in this gene lead to metachromatic leucodystrophy (MLD), a progressive demyelination disease which results in a variety of neurological symptoms and ultimately death. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Dec 2010]
Transcript Variant: This variant (1) represents the longest transcript and it encodes the longer protein (isoform a). Variants 1, 2, 3, and 4 encode the same isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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