p53 (TP53) (NM_000546) Human Untagged Clone

Name: p53 (TP53) (NM_000546) Human Untagged Clone
SKU: SC119832
Price: 5488.00

CAT＃: SC119832

TP53 (untagged)-Human tumor protein p53 (TP53), transcript variant 1

Datasheet

SDS

"NM_000546" in other vectors (6)

CNY 5,488.00

货期*

现货

规格

Cited in 13 publications.

Product images

经常一起买 (4)

TA502870

TP53 mouse monoclonal antibody, clone OTI5E2 (formerly 5E2)

CNY 1,999.00
CNY 2,700.00

【特别福利】购一抗正装产品（≥50ul或≥50ug），免费领10ul抗体试用装

TF81001

TurboFectin Transfection Reagent (1 mL in 1 vial)

CNY 4,090.00

CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

CNY 1,280.00

PRIMER

Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each

CNY 480.00

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	BCC7; BMFS5; LFS1; P53; TRP53
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF sequence for NM_000546 edited ATGGAGGAGCCGCAGTCAGATCCTAGCGTCGAGCCCCCTCTGAGTCAGGAAACATTTTCA GACCTATGGAAACTACTTCCTGAAAACAACGTTCTGTCCCCCTTGCCGTCCCAAGCAATG GATGATTTGATGCTGTCCCCGGACGATATTGAACAATGGTTCACTGAAGACCCAGGTCCA GATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCGCGTGGCCCCTGCACCAGCAGCTCCT ACACCGGCGGCCCCTGCACCAGCCCCCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAG AAAACCTACCAGGGCAGCTACGGTTTCCGTCTGGGCTTCTTGCATTCTGGGACAGCCAAG TCTGTGACTTGCACGTACTCCCCTGCCCTCAACAAGATGTTTTGCCAACTGGCCAAGACC TGCCCTGTGCAGCTGTGGGTTGATTCCACACCCCCGCCCGGCACCCGCGTCCGCGCCATG GCCATCTACAAGCAGTCACAGCACATGACGGAGGTTGTGAGGCGCTGCCCCCACCATGAG CGCTGCTCAGATAGCGATGGTCTGGCCCCTCCTCAGCATCTTATCCGAGTGGAAGGAAAT TTGCGTGTGGAGTATTTGGATGACAGAAACACTTTTCGACATAGTGTGGTGGTGCCCTAT GAGCCGCCTGAGGTTGGCTCTGACTGTACCACCATCCACTACAACTACATGTGTAACAGT TCCTGCATGGGCGGCATGAACCGGAGGCCCATCCTCACCATCATCACACTGGAAGACTCC AGTGGTAATCTACTGGGACGGAACAGCTTTGAGGTGCGTGTTTGTGCCTGTCCTGGGAGA GACCGGCGCACAGAGGAAGAGAATCTCCGCAAGAAAGGGGAGCCTCACCACGAGCTGCCC CCAGGGAGCACTAAGCGAGCACTGCCCAACAACACCAGCTCCTCTCCCCAGCCAAAGAAG AAACCACTGGATGGAGAATATTTCACCCTTCAGATCCGTGGGCGTGAGCGCTTCGAGATG TTCCGAGAGCTGAATGAGGCCTTGGAACTCAAGGATGCCCAGGCTGGGAAGGAGCCAGGG GGGAGCAGGGCTCACTCCAGCCACCTGAAGTCCAAAAAGGGTCAGTCTACCTCCCGCCAT AAAAAACTCATGTTCAAGACAGAAGGGCCTGACTCAGACTGA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_000546 unedited TTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTTTGCGTTCGGGC TGGGAGCGTGCTTTCCACGACGGTGACACGCTTCCCTGGATTGGCAGCCAGACTGCCTTC CGGGTCACTGCCATGGAGGAGCCGCAGTCAGATCCTAGCGTCGAGCCCCCTCTGAGTCAG GAAACATTTTCAGACCTATGGAAACTACTTCCTGAAAACAACGTTCTGTCCCCCTTGCCG TCCCAAGCAATGGATGATTTGATGCTGTCCCCGGACGATATTGAACAATGGTTCACTGAA GACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCGCGTGGCCCCTGCA CCAGCAGCTCCTACACCGGCGGCCCCTGCACCAGCCCCCTCCTGGCCCCTGTCATCTTCT GTCCCTTCCCAGAAAACCTACCAGGGCAGCTACGGTTTCCGTCTGGGCTTCTTGCATTCT GGGACAGCCAAGTCTGTGACTTGCACGTACTCCCCTGCCCTCAACAAGATGTTTTGCCAA CTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCCCGCCCGGCACCCGC GTCCGCGCCATGGCCCATCTACAAGCAGTCACAGCACATGACGGAGGTTGTGAGGCGCTG CCCCCACCATGAGCGCTGCTCAGATAGCGATGGTCTGCCCCTCCTCAGCATCTTATCCGA GTGGAAAGAAATTTGGCGTGTGGAGTATTTGGATGACAGAAAACACTTTTCCACATATGG GNGTGGTCGCCCTAAGAGCCCCCCTGAGGTTGGGTCTGACTGTCCCACCATCCCTTCCAC TAACTGGGAAACAGTTCTTGCTTGGCCGGGATGAACCGGAAGGCCCTCCTTACCTCATCA CACTGGAAGACTCCGGGGAATTCACG 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for NM_000546 unedited CCGCGGCCGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTGGCAGCAAAGTTTTATTG TAAAATAAGAGATCGATATAAAAATGGGATATAAAAAGGGAGAAGGAGGGGAAGGGTGGG GTGAAAATGCAGATGTGCTTGCAGAATGTAAAAGATGTTGACCCTTCCAGCTGGACGTGG TGGCTCACAATTGTAATCCCAGCACTCTGGGAGGCTGAGACAGGTGGATCGCCTGAGCCC AGGAGTTTGAGACCAGCCTGGGCAACACTGTGAGACCCCATCTCTACAAAACATGCAAAA GTTGGCTGGCCATGGTGGCATGAACCTGTGGTCCCAGCTACTCCGGAGGCTGAGGCAGGA CTGCTCGAGCCGGGGAGGCAAAGGCTGCAGTAAGCCAAGATCACGCCACTCCACTCCAGT CTGGGCAACTAAGCGAGACCCAGTCTTCAAGTAATTAGACCCCTTTCATTCCACTCCAAA TCCGATTTTGACCCTGAGCCTATAACAAGTCTTCGGTGGATCCCGCACCATCCTATACAA AAACATGAAATTCTTCAAGGGTGTGGGATGCGGGTGACAATTTCCCTTTTACGGTTCCAA CGGTTACCCAACAGGTTGGCCCACAATGGTTTGTGCTGGGCCCCTCATAACACTTGATAA CTCCCCTCTTACTTAACCACGTTTCACAAATACGTATATACTCCTCCCACCCCTCTTCAC ACCATCGGCTATGGGCACCCCCCCTCCGTCAAGGCGCGTCTACTCCCTCCCCCCACCTCT CACATAACAGTCTCTACACCCGAGTCTCACTCCTCTTCTATTTATTTCCTCCTCCACCCC GCTCTCCTGCCCGTCTGCTTCCCCATTCTACCCCTCCCCTCCCTCAATTATTACCCCTCT CTCTCACTCTATAACTCCCAAAGTGCCATGCCACACATTTATCTTCTCCCGTTCTACCCG CCACAGTTCACTCTCTCCGTATAATCGCATTCTAAACATCCGTCGCTCACCCCC
Restriction Sites	NotI-NotI
ACCN	NM_000546
Insert Size	1182 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_000546.2, NP_000537.2
RefSeq Size	2629 bp
RefSeq ORF	1182 bp
Locus ID	7157
UniProt ID	P04637
Domains	P53
Protein Families	Druggable Genome, Stem cell - Pluripotency, Transcription Factors
Protein Pathways	Amyotrophic lateral sclerosis (ALS), Apoptosis, Basal cell carcinoma, Bladder cancer, Cell cycle, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, Glioma, Huntington's disease, MAPK signaling pathway, Melanoma, Neurotrophin signaling pathway, Non-small cell lung cancer, p53 signaling pathway, Pancreatic cancer, Pathways in cancer, Prostate cancer, Small cell lung cancer, Thyroid cancer, Wnt signaling pathway
Gene Summary	This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277). [provided by RefSeq, Dec 2016] Transcript Variant: This variant (1) can initiate translation from two in-frame AUG start codons. The isoform represented in this variant (a, also known as p53alpha) results from translation initiation at the upstream start codon. Both variants 1 and 2 encode isoform a, which is the longest isoform.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (13)

The use of this cDNA Clones has been cited in the following citations:
iNOS/NO is required for IRF1 activation in response to liver ischemia-reperfusion in mice ,Du, Q;Luo, J;Yang, MQ;Liu, Q;Heres, C;Yan, YH;Stolz, D;Geller, DA;, Mol. Med. ,PubMed ID 32517688 [p53]
Length and secondary structure of the 5\' non-coding regions of mouse p53 mRNA transcripts - mouse as a model organism for p53 gene expression studies ,Szpotkowska, J;Swiatkowska, A;Ciesiołka, J;, RNA Biol ,PubMed ID 30518296 [p53]
CELF1/p53 axis: a sustained antiproliferative signal leading to villus atrophy under total parenteral nutrition ,Yan, JK;Zhang, T;Dai, LN;Gu, BL;Zhu, J;Yan, WH;Cai, W;Wang, Y;, FASEB J. ,PubMed ID 30514107 [p53]
HMGB1 represses the anti-cancer activity of sunitinib by governing TP53 autophagic degradation via its nucleus-to-cytoplasm transport ,Luo, P;Xu, Z;Li, G;Yan, H;Zhu, Y;Zhu, H;Ma, S;Yang, B;He, Q;, Autophagy ,PubMed ID 30205729 [p53]
Transcribed ultraconserved region 339 promotes carcinogenesis by modulating tumor suppressor microRNAs ,Vannini, I;Wise, PM;Challagundla, KB;Plousiou, M;Raffini, M;Bandini, E;Fanini, F;Paliaga, G;Crawford, M;Ferracin, M;Ivan, C;Fabris, L;Davuluri, RV;Guo, Z;Cortez, MA;Zhang, X;Chen, L;Zhang, S;Fernandez-Cymering, C;Han, L;Carloni, S;Salvi, S;Ling, H;Murtadha, M;Neviani, P;Gitlitz, BJ;Laird-Offringa, IA;Nana-Sinkam, P;Negrini, M;Liang, H;Amadori, D;Cimmino, A;Fabbri, M;Calin, GA;, Nat Commun ,PubMed ID 29180617 [p53]
Molecular mechanism leading to SAHA-induced autophagy in tumor cells: evidence for a p53-dependent pathway ,Fröhlich, LF;Mrakovcic, M;Smole, C;Zatloukal, K;, Cancer Cell Int. ,PubMed ID 27601937 [p53]
Loss of Abhd5 Promotes Colorectal Tumor Development and Progression by Inducing Aerobic Glycolysis and Epithelial-Mesenchymal Transition ,null, Cell reports ,PubMed ID 25482557 [p53]
Cytosolic galectin-7 impairs p53 functions and induces chemoresistance in breast cancer cells ,Grosset, AA;Labrie, M;Gagné, D;Vladoiu, MC;Gaboury, L;Doucet, N;St-Pierre, Y;, BMC Cancer ,PubMed ID 25367122 [p53]
SCO2 Induces p53-Mediated Apoptosis by Thr845 Phosphorylation of ASK-1 and Dissociation of the ASK-1–Trx Complex ,Esha Madan, Rajan Gogna, Periannan Kuppusamy, Madan Bhatt, Abbas Ali Mahdi, and Uttam Pati, Mol. Cell. Biol., Apr 2013; 33: 1285 - 1302. ,PubMed ID 23319048 [p53]
miR-34a contributes to megakaryocytic differentiation of K562 cells independently of p53 ,Francisco Navarro, David Gutman, Eti Meire, Mario Caceres, Isidore Rigoutsos, Zvi Bentwich, and Judy Lieberman, Blood, Jul 2009; 10.1182/blood-2009-02-205062. [p53]
Interaction of Heterogeneous Nuclear Ribonucleoprotein C1/C2 with a Novel cis-Regulatory Element within p53 mRNA as a Response to Cytostatic Drug Treatment ,Kyle J. Christian, Matti A. Lang, and Françoise Raffalli-Mathieu, Mol. Pharmacol., May 2008; 73: 1558 - 1567. [p53]
Macrophages Produce TGF-ß-Induced (ß-ig-h3) following Ingestion of Apoptotic Cells and Regulate MMP14 Levels and Collagen Turnover in Fibroblasts ,Zachary A. Cooper, Michael P. Gillmeister, Nevins W. Todd, and Sergei P. Atamas, J. Cell Sci., Apr 2008; 121: 989 - 1001. [p53]
Interaction of hnRNPC1/C2 with a novel cis-regulatory element within p53 mRNA as a response to cytostatic drug treatment ,Kyle J Christian, Matti A. Lang, and Francoise Raffali-Mathieu, Mol. Pharmacol., Feb 2008; 10.1124/mol.107.042507. [p53]

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
RC200003	TP53 (Myc-DDK-tagged)-Human tumor protein p53 (TP53), transcript variant 1	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,656.00 CNY 5,320.00
RC200003L1	Lenti ORF clone of Human tumor protein p53 (TP53), transcript variant 1, Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 6,056.00
RC200003L2	Lenti ORF clone of Human tumor protein p53 (TP53), transcript variant 1, mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 6,056.00
RC200003L3	Lenti ORF clone of Human tumor protein p53 (TP53), transcript variant 1, Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 6,056.00
RC200003L4	Lenti ORF clone of Human tumor protein p53 (TP53), transcript variant 1, mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 6,056.00
RG200003	TP53 (tGFP-tagged) - Human tumor protein p53 (TP53), transcript variant 1	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,256.00

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