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Acid sphingomyelinase (SMPD1) (NM_000543) Human Untagged Clone
CAT＃: SC119827
SMPD1 (untagged)-Human sphingomyelin phosphodiesterase 1, acid lysosomal (SMPD1), transcript variant 1
CNY 4,704.00
| Cited in 1 publication. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ASM; ASMASE; NPD |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC119827 sequence for NM_000543 edited (data generated by NextGen Sequencing)
ATGCCCCGCTACGGAGCGTCACTCCGCCAGAGCTGCCCCAGGTCCGGCCGGGAGCAGGGA CAAGACGGGACCGCCGGAGCCCCCGGACTCCTTTGGATGGGCCTGGCGCTGGCGCTGGCG CTGGCGCTGGCGCTGGCGCTGGCTCTGTCTGACTCTCGGGTTCTCTGGGCTCCGGCAGAG GCTCACCCTCTTTCTCCCCAAGGCCATCCTGCCAGGTTACATCGCATAGTGCCCCGGCTC CGAGATGTCTTTGGGTGGGGGAACCTCACCTGCCCAATCTGCAAAGGTCTATTCACCGCC ATCAACCTCGGGCTGAAGAAGGAACCCAATGTGGCTCGCGTGGGCTCCGTGGCCATCAAG CTGTGCAATCTGCTGAAGATAGCACCACCTGCCGTGTGCCAATCCATTGTCCACCTCTTT GAGGATGACATGGTGGAGGTGTGGAGACGCTCAGTGCTGAGCCCATCTGAGGCCTGTGGC CTGCTCCTGGGCTCCACCTGTGGGCACTGGGACATTTTCTCATCTTGGAACATCTCTTTG CCTACTGTGCCGAAGCCGCCCCCCAAACCCCCTAGCCCCCCAGCCCCAGGTGCCCCTGTC AGCCGCATCCTCTTCCTCACTGACCTGCACTGGGATCATGACTACCTGGAGGGCACGGAC CCTGACTGTGCAGACCCACTGTGCTGCCGCCGGGGTTCTGGCCTGCCGCCCGCATCCCGG CCAGGTGCCGGATACTGGGGCGAATACAGCAAGTGTGACCTGCCCCTGAGGACCCTGGAG AGCCTGTTGAGTGGGCTGGGCCCAGCCGGCCCTTTTGATATGGTGTACTGGACAGGAGAC ATCCCCGCACATGATGTCTGGCACCAGACTCGTCAGGACCAACTGCGGGCCCTGACCACC GTCACAGCACTTGTGAGGAAGTTCCTGGGGCCAGTGCCAGTGTACCCTGCTGTGGGTAAC CATGAAAGCACACCTGTCAATAGCTTCCCTCCCCCCTTCATTGAGGGCAACCACTCCTCC CGCTGGCTCTATGAAGCGATGGCCAAGGCTTGGGAGCCCTGGCTGCCTGCCGAAGCCCTG CGCACCCTCAGAATTGGGGGGTTCTATGCTCTTTCCCCATACCCCGGTCTCCGCCTCATC TCTCTCAATATGAATTTTTGTTCCCGTGAGAACTTCTGGCTCTTGATCAACTCCACGGAT CCCGCAGGACAGCTCCAGTGGCTGGTGGGGGAGCTTCAGGCTGCTGAGGATCGAGGAGAC AAAGTGCATATAATTGGCCACATTCCCCCAGGGCACTGTCTGAAGAGCTGGAGCTGGAAT TATTACCGAATTGTAGCCAGGTATGAGAACACCCTGGCTGCTCAGTTCTTTGGCCACACT CATGTGGATGAATTTGAGGTCTTCTATGATGAAGAGACTCTGAGCCGGCCGCTGGCTGTA GCCTTCCTGGCACCCAGTGCAACTACCTACATCGGCCTTAATCCTGGTTACCGTGTGTAC CAAATAGATGGAAACTACTCCGGGAGCTCTCACGTGGTCCTGGACCATGAGACCTACATC CTGAATCTGACCCAGGCAAACATACCGGGAGCCATACCGCACTGGCAGCTTCTCTACAGG GCTCGAGAAACCTATGGGCTGCCCAACACACTGCCTACCGCCTGGCACAACCTGGTATAT CGCATGCGGGGCGACATGCAACTTTTCCAGACCTTCTGGTTTCTCTACCATAAGGGCCAC CCACCCTCGGAGCCCTGTGGCACGCCCTGCCGTCTGGCTACTCTTTGTGCCCAGCTCTCT GCCCGTGCTGACAGCCCTGCTCTGTGCCGCCACCTGATGCCAGATGGGAGCCTCCCAGAG GCCCAGAGCCTGTGGCCAAGGCCACTGTTTTGCTAG Clone variation with respect to NM_000543.4 107 t=>c >OriGene 5' read for NM_000543 unedited
GTTGACTATTTTGTAATACGACTTTACTATAGGGCGGCCGCGAATTCGCACGAGGGGACA GAGAGAGGAAGAGGAAGGGGCGGAGCTGCTTTGCGGCCGGCCGCGGAGCAGTCAGCCGAC TACAGAGAAGGGTAATCGGGTGTCCCCGGCGCCGCCCGGGGCCCTGAGGGCTGGCTAGGG TCCAGGCCGGGGGGGACGGGACAGACGAACCAGCCCCGTGTAGGAAGCGCGACAATGCCC CGCTACGGAGCGTCACTCCGCCAGAGCTGCCCCAGGTCCGGCCGGGAGCAGGGACAAGAC GGGACCGCCGGAGCCCCCGGACTCCTTTGGATGGGCCTGGCGCTGGCGCTGGCGCTGGCG CTGGCGCTGGCGCTGGCTCTGTCTGACTCTCGGGTTCTCTGGGCTCCGGCAGAGGCTCAC CCTCTTTCTCCCCAAGGCCATCCTGCCAGGTTACATCGCATAGTGCCCCGGCTCCGAGAT GTCTTTGGGTGGGGGAACCTCACCTGCCCAATCTGCAAAGGTCTATTCACCGCCATCAAC CTCGGGCTGAAGAAGGAACCCAATGTGGCTCGCGTGGGCTCCGTGGCCATCAAGCTGTGC AATCTGCTGAAGATAGCACCACCTGCCGTGTGCCAATCCATTGTCCACCTCTTTGAGGAT GACATGGTGGAGGTGTGGAGACGCTCAGTGCTGAGCCCATCTGAGGCCTGTGGCCTGCTC CTGGGCTCCACCTGTGGGCACTGGGACATTTTCTCATCTTGGAACATCTCTTTGCCTACT GGCCGAAAACCCGCCCCCCCAAACCCCCTAGCCCCCCAACCCCAAGTGGCCCCTGGCACC CGCATCCTCTTCTCACTGACCTGCACTGGGATCATGACTACCTGGGAGGCCACGGACCCT GATTGTGCAAACACACTGTTGTTGCCCCCGG >OriGene 3' read for NM_000543 unedited
GCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGGAGTCCAAGTCTCTTATCTTTA TTTTAACAGACTGGCAGCATCAGGTCGCAGCAGCAGTACAGGGTTCCTGGCTGGGCAGCA CAGGCCTGGGGCGACAGCTCCTGTCTTGTCTGCCCAGGGCAGTGTCAACTTAAGCCTCTA CTCCATGGCTGTGAAAGGACAGCAGCCTCAAGGCAGTCTAGCTCCTGGGCACAGGCAGCC AAACCCCCTCCCATATCCAGCTAAACCAGCTCCAGGAAAGGAGAAGGTCCTGTTTCCCCG GCATCCTTGGGGCCCAGGGACTGGTTCTTTCACCGGATGATCTTGCCTGGTTGAACCACA GCAGCATTTGGGCTTTTTCACCCTTTCCTACATCAAGAACTTTCCCAAATGTGGGCCCTG GGGCCCTAGCAAAACAGTGGCCTTGGCCACAGGCTCTGGGCCTCTGGGAGGCTCCCATCT GGCATCAGGTGGCGGCACAGAGCAGGGCTGTCAGCACGGGCAGAGAGCTGGGCACAAAGA GTAGCCAGACGGCAGGGCGTGCCACAGGGCTCCGAGGGTGGGTGGCCCTTATGGTAGAGA AACCAGAAGGTCTGGAAAAGTTGCATGTCGCCCCGCATGCGATATACCATGTTGTGCCAG GCGGTATGCAGTGTGTTGGGCAGCCCATATGTTTCTCGAGCCCTGTAGAGAATCTGNCAG TGCGGTATGGCTCCCCGTATGTTTGCCTGGGTCAAATTCANGATGTAGGTCTCATGGTCC CAGAACACGTGAGATGCTCCGGAGTAGTTTCCATCTATTTGGTACACACGGTACCCAGAT TAAGCCGATGTAAGTAGTTGCACTGGGTGCCANGAAAGCTACAGCCANNCGCCGGCTCAA AGTCTCTTCTCATAGAAGACCTCCAATCCTCCACCATGATGTTGGCCAAGAACTGAGCGC NCAGGGGTGGTCTCATACCTGGCTACANTNCNGNNNATANTCCACNTCCAC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_000543 |
| Insert Size | 2640 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000543.3, NP_000534.3 |
| RefSeq Size | 2473 bp |
| RefSeq ORF | 1896 bp |
| Locus ID | 6609 |
| UniProt ID | P17405 |
| Domains | Metallophos, SAPB |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | Lysosome, Metabolic pathways, Sphingolipid metabolism |
| Gene Summary | The protein encoded by this gene is a lysosomal acid sphingomyelinase that converts sphingomyelin to ceramide. The encoded protein also has phospholipase C activity. Defects in this gene are a cause of Niemann-Pick disease type A (NPA) and Niemann-Pick disease type B (NPB). Multiple transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2010] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Ebolavirus Requires Acid Sphingomyelinase Activity and Plasma Membrane Sphingomyelin for Infection
,Mary E. Miller, Shramika Adhikary, Andrey A. Kolokoltsov, and Robert A. Davey,
J. Virol., Jul 2012; 86: 7473 - 7483.
[SMPD1]
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219758 | SMPD1 (Myc-DDK-tagged)-Human sphingomyelin phosphodiesterase 1, acid lysosomal (SMPD1), transcript variant 1 |
CNY 4,696.00 |
|
| RC219758L1 | Lenti ORF clone of Human sphingomyelin phosphodiesterase 1, acid lysosomal (SMPD1), transcript variant 1, Myc-DDK-tagged |
CNY 7,096.00 |
|
| RC219758L2 | Lenti ORF clone of Human sphingomyelin phosphodiesterase 1, acid lysosomal (SMPD1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC219758L3 | Lenti ORF clone of Human sphingomyelin phosphodiesterase 1, acid lysosomal (SMPD1), transcript variant 1, Myc-DDK-tagged |
CNY 7,096.00 |
|
| RC219758L4 | Lenti ORF clone of Human sphingomyelin phosphodiesterase 1, acid lysosomal (SMPD1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG219758 | SMPD1 (tGFP-tagged) - Human sphingomyelin phosphodiesterase 1, acid lysosomal (SMPD1), transcript variant 1 |
CNY 6,296.00 |
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