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PCCB (NM_000532) Human Untagged Clone
CAT＃: SC119822
PCCB (untagged)-Human propionyl CoA carboxylase, beta polypeptide (PCCB), nuclear gene encoding mitochondrial protein, transcript variant 1
CNY 4,024.00
CNY 7,220.00
Product images
CNY 6,281.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC119822 sequence for NM_000532 edited (data generated by NextGen Sequencing)
ATGGCGGCGGCATTACGGGTGGCGGCGGTCGGGGCAAGGCTCAGCGTTCTGGCGAGCGGT CTCCGCGCCGCGGTCCGCAGCCTTTGCAGCCAGGCCACCTCTGTTAACGAACGCATCGAA AACAAGCGCCGGACCGCGCTGCTGGGAGGGGGCCAACGCCGTATTGACGCGCAGCACAAG CGAGGAAAGCTAACAGCCAGGGAGAGGATCAGTCTCTTGCTGGACCCTGGCAGCTTTGTT GAGAGCGACATGTTTGTGGAACACAGATGTGCAGATTTTGGAATGGCTGCTGATAAGAAT AAGTTTCCTGGAGACAGCGTGGTCACTGGACGAGGCCGAATCAATGGAAGATTGGTTTAT GTCTTCAGTCAGGATTTTACAGTTTTTGGAGGCAGTCTGTCAGGAGCACATGCCCAAAAG ATCTGCAAAATCATGGACCAGGCCATAACGGTGGGGGCTCCAGTGATTGGGCTGAATGAC TCTGGGGGAGCACGGATCCAAGAAGGAGTGGAGTCTTTGGCTGGCTATGCAGACATCTTT CTGAGGAATGTTACGGCATCCGGAGTCATCCCTCAGATTTCTCTGATCATGGGCCCATGT GCTGGTGGGGCCGTCTACTCCCCAGCCCTAACAGACTTCACGTTCATGGTAAAGGACACC TCCTACCTGTTCATCACTGGCCCTGATGTTGTGAAGTCTGTCACCAATGAGGATGTTACC CAGGAGGAGCTCGGTGGTGCCAAGACCCACACCACCATGTCAGGTGTGGCCCACAGAGCT TTTGAAAATGATGTTGATGCCTTGTGTAATCTCCGGGATTTCTTCAACTACCTGCCCCTG AGCAGTCAGGACCCGGCTCCCGTCCGTGAGTGCCACGATCCCAGTGACCGTCTGGTTCCT GAGCTTGACACAATTGTCCCTTTGGAATCAACCAAAGCCTACAACATGGTGGACATCATA CACTCTGTTGTTGATGAGCGTGAATTTTTTGAGATCATGCCCAATTATGCCAAGAACATC ATTGTTGGTTTTGCAAGAATGAATGGGAGGACTGTTGGAATTGTTGGCAACCAACCTAAG GTGGCCTCAGGATGCTTGGATATTAATTCATCTGTGAAAGGGGCTCGTTTTGTCAGATTC TGTGATGCATTCAATATTCCACTCATCACTTTTGTTGATGTCCCTGGCTTTCTACCTGGC ACAGCACAGGAATACGGGGGCATCATCCGGCATGGTGCCAAGCTTCTCTACGCATTTGCT GAGGCAACTGTACCCAAAGTCACAGTCATCACCAGGAAGGCCTATGGAGGTGCCTATGAT GTCATGAGCTCTAAGCACCTTTGTGGTGATACCAACTATGCCTGGCCCACCGCAGAGATT GCAGTCATGGGAGCAAAGGGCGCTGTGGAGATCATCTTCAAAGGGCATGAGAATGTGGAA GCTGCTCAGGCAGAGTACATCGAGAAGTTTGCCAACCCTTTCCCTGCAGCAGTGCGAGGG TTTGTGGATGACATCATCCAACCTTCTTCCACACGTGCCCGAATCTGCTGTGACCTGGAT GTCTTGGCCAGCAAGAAGGTACAACGTCCTTGGAGAAAACATGCAAATATTCCATTGTAA Clone variation with respect to NM_000532.4 >OriGene 5' read for NM_000532 unedited
GGTCAAAATTTGTATACGACTCCTATAGGGCGGCCGCGATTCGGCACGAGGCCTCGTGCC GAATTCGGCACGAGGGCCGGCACAGCAAAAAGGCGGCGGCATTACGGGTGGCGGCGGTCG GGGCAAGGCTCAGCGTTCTGGCGAGCGGTCTCCGCGCCGCGGTCCGCAGCCTTTGCAGCC AGGCCACCTCTGTTAACGAACGCATCGAAAACAAGCGCCGGACCGCGCTGCTGGGAGGGG GCCAACGCCGTATTGACGCGCAGCACAAGCGAGGAAAGCTAACAGCCAGGGAGAGGATCA GTCTCTTGCTGGACCCTGGCAGCTTTGTTGAGAGCGACATGTTTGTGGAACACAGATGTG CAGATTTTGGAATGGCTGCTGATAAGAATAAGTTTCCTGGAGACAGCGTGGTCACTGGAC GAGGCCGAATCAATGGAAGATTGGTTTATGTCTTCAGTCAGGATTTTACAGTTTTTGGAG GCAGTCTGTCAGGAGCACATGCCCAAAAGATCTGCAAAATCATGGACCAGGCCATAACGG TGGGGGCTCCAGTGATTGGGCTGAATGACTCTGGGGGAGCACGGATCCAAGAAGGAGTGG AGTCTTTGGCTGGCTATGCAGACATCTTTCTGAGGAATGTTACGGCATCCGGAGTCATCC CTCAGATTTCTCTGATCATGGGCCCATGTGCTGGTGGGGCCGTCTACTCCCCAGCCCTAA CAGACTTCACGTTCATGGTAAAGGACACCTCCTACCTGTTCATCACTGGCCCTGATGTTG TGAAGTCTGTCACCATGGAAGATGTTACCCAGGAAGAGCCTGGGTGGTGCCAAGACCCCA CCACCATGTCAGGTGTGCCCACAGAAGCTTTGAAAAAGAAGGTGAAGCCTTGTGTAACTC TCGGGATTT >OriGene 3' read for NM_000532 unedited
GGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTACTATTTTTCTTTCTAGTTGTA TTGATTTTTCAGATTTCCACCTAAAAAAATCGAATTTATGCTCAAAAAATACAACTTAAA ATGTTTAAAAAAGTAAATAATTTTACAGTAAGGCACAAAAGAGATCTTTCTAGAATTTAA TAAACTTAGTTATTCTAAGTTATCCAACTATTTGGATTCCCAGGTTTCATGATTGCAAAA GGCAGGAATGGGATGTGAATGGGCAGACAGTAATTCAGTTCTTGGTTTCTTTTCCTTTGA TTTGTTTACAATGGAATATTTGCATGTTTTCTCCAAGGACGTTGTACCTTCTTGCTGGCC AAGACATCCAGGTCACAGCAGATTCGGGCACGTGTGGAAGAAGGTTGGATGATGTCATCC ACAAACCCTCGCACTGCTGCAGGGAAAGGGTTGGCAAACTTCTCGATGTACTCTGCCTGA GCAGCTTCCACATTCTCATGCCCTTTGAAGATGATCTCCACAGCGCCCTTTGCTCCCATG ACTGCAATCTCTGCGGTGGGCCAGGCATAGTTGGTATCACCACAAAGGTGCTTAGAGCTC ATGACATCATAGGCACCTCCATAGGCCTTCCTGGTGATGACTGTGACTTTGGGTACAGTT GCCTCAGCAAATGCGTAGAGAAGCTTGGCACCATGCCGGATGATGCCCCCGTATTCCTGT GCTGTGCCAGGTAGAAAGCCAGGGACATCAACAAAAGTGATGAGTGGGATATTGAATGCA TCACAGAATCTGACAAAACGAGCCCCTTTCACAGATGAAATATATTCAGCATCCTGAGGG CCACTTAGGTTGGTTGCCACAATNNCACAGTNNCTCCATCATTCTTGCAAAACAACATGA TGGTTTGGGCATATGGGCATGATCTACAAAATCACGCTATAACACACGAGGATGATGTCA CCTGTTGNAGCCTTGGTGATCCCAAGAC |
| Restriction Sites | ECoRI-NOT |
| ACCN | NM_000532 |
| Insert Size | 1960 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000532.2, NP_000523.1 |
| RefSeq Size | 1829 bp |
| RefSeq ORF | 1620 bp |
| Locus ID | 5096 |
| UniProt ID | P05166 |
| Domains | Carboxyl_trans |
| Protein Families | Druggable Genome |
| Protein Pathways | Metabolic pathways, Propanoate metabolism, Valine, leucine and isoleucine degradation |
| Gene Summary | The protein encoded by this gene is a subunit of the propionyl-CoA carboxylase (PCC) enzyme, which is involved in the catabolism of propionyl-CoA. PCC is a mitochondrial enzyme that probably acts as a dodecamer of six alpha subunits and six beta subunits. This gene encodes the beta subunit of PCC. Defects in this gene are a cause of propionic acidemia type II (PA-2). Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2010] Transcript Variant: This variant (1) represents the shorter transcript and encodes the shorter isoform (1). |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202019 | PCCB (Myc-DDK-tagged)-Human propionyl CoA carboxylase, beta polypeptide (PCCB), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 4,016.00 |
|
| RC202019L1 | Lenti ORF clone of Human propionyl CoA carboxylase, beta polypeptide (PCCB), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged |
CNY 6,416.00 |
|
| RC202019L2 | Lenti ORF clone of Human propionyl CoA carboxylase, beta polypeptide (PCCB), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged |
CNY 6,460.00 |
|
| RC202019L3 | Lenti ORF clone of Human propionyl CoA carboxylase, beta polypeptide (PCCB), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC202019L4 | Lenti ORF clone of Human propionyl CoA carboxylase, beta polypeptide (PCCB), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged |
CNY 6,460.00 |
|
| RG202019 | PCCB (tGFP-tagged) - Human propionyl CoA carboxylase, beta polypeptide (PCCB), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 5,616.00 |
|
| SC323905 | PCCB (untagged)-Human propionyl CoA carboxylase, beta polypeptide (PCCB), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 4,024.00 |
