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HEXA (NM_000520) Human Untagged Clone
CAT＃: SC119813
HEXA (untagged)-Human hexosaminidase A (alpha polypeptide) (HEXA)
CNY 3,944.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | TSD |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC119813 sequence for NM_000520 edited (data generated by NextGen Sequencing)
ATGACAAGCTCCAGGCTTTGGTTTTCGCTGCTGCTGGCGGCAGCGTTCGCAGGACGGGCG ACGGCCCTCTGGCCCTGGCCTCAGAACTTCCAAACCTCCGACCAGCGCTACGTCCTTTAC CCGAACAACTTTCAATTCCAGTACGATGTCAGCTCGGCCGCGCAGCCCGGCTGCTCAGTC CTCGACGAGGCCTTCCAGCGCTATCGTGACCTGCTTTTCGGTTCCGGGTCTTGGCCCCGT CCTTACCTCACAGGGAAACGGCATACACTGGAGAAGAATGTGTTGGTTGTCTCTGTAGTC ACACCTGGATGTAACCAGCTTCCTACTTTGGAGTCAGTGGAGAATTATACCCTGACCATA AATGATGACCAGTGTTTACTCCTCTCTGAGACTGTCTGGGGAGCTCTCCGAGGTCTGGAG ACTTTTAGCCAGCTTGTTTGGAAATCTGCTGAGGGCACATTCTTTATCAACAAGACTGAG ATTGAGGACTTTCCCCGCTTTCCTCACCGGGGCTTGCTGTTGGATACATCTCGCCATTAC CTGCCACTCTCTAGCATCCTGGACACTCTGGATGTCATGGCGTACAATAAATTGAACGTG TTCCACTGGCATCTGGTAGATGATCCTTCCTTCCCATATGAGAGCTTCACTTTTCCAGAG CTCATGAGAAAGGGGTCCTACAACCCTGTCACCCACATCTACACAGCACAGGATGTGAAG GAGGTCATTGAATACGCACGGCTCCGGGGTATCCGTGTGCTTGCAGAGTTTGACACTCCT GGCCACACTTTGTCCTGGGGACCAGGTATCCCTGGATTACTGACTCCTTGCTACTCTGGG TCTGAGCCCTCTGGCACCTTTGGACCAGTGAATCCCAGTCTCAATAATACCTATGAGTTC ATGAGCACATTCTTCTTAGAAGTCAGCTCTGTCTTCCCAGATTTTTATCTTCATCTTGGA GGAGATGAGGTTGATTTCACCTGCTGGAAGTCCAACCCAGAGATCCAGGACTTTATGAGG AAGAAAGGCTTCGGTGAGGACTTCAAGCAGCTGGAGTCCTTCTACATCCAGACGCTGCTG GACATCGTCTCTTCTTATGGCAAGGGCTATGTGGTGTGGCAGGAGGTGTTTGATAATAAA GTAAAGATTCAGCCAGACACAATCATACAGGTGTGGCGAGAGGATATTCCAGTGAACTAT ATGAAGGAGCTGGAACTGGTCACCAAGGCCGGCTTCCGGGCCCTTCTCTCTGCCCCCTGG TACCTGAACCGTATATCCTATGGCCCTGACTGGAAGGATTTCTACGTAGTGGAACCCCTG GCATTTGAAGGTACCCCTGAGCAGAAGGCTCTGGTGATTGGTGGAGAGGCTTGTATGTGG GGAGAATATGTGGACAACACAAACCTGGTCCCCAGGCTCTGGCCCAGAGCAGGGGCTGTT GCCGAAAGGCTGTGGAGCAACAAGTTGACATCTGACCTGACATTTGCCTATGAACGTTTG TCACACTTCCGCTGTGAGTTGCTGAGGCGAGGTGTCCAGGCCCAACCCCTCAATGTAGGC TTCTGTGAGCAGGAGTTTGAACAGACCTGA Clone variation with respect to NM_000520.4 1306 a=>g;1518 a=>g >OriGene 5' read for NM_000520 unedited
NNNNNTTGTCAGAAATTGTATACGACTCACTATAGGCGGCCGCGAATCGGCACGAGGGAC CAGCGGGCCATGACAAGCTCCAGGCTTTGGTTTTCGCTGCTGCTGGCGGCAGCGTTCGCA GGACGGGCGACGGCCCTCTGGCCCTGGCCTCAGAACTTCCAAACCTCCGACCAGCGCTAC GTCCTTTACCCGAACAACTTTCAATTCCAGTACGATGTCAGCTCGGCCGCGCAGCCCGGC TGCTCAGTCCTCGACGAGGCCTTCCAGCGCTATCGTGACCTGCTTTTCGGTTCCGGGTCT TGGCCCCGTCCTTACCTCACAGGGAAACGGCATACACTGGAGAAGAATGTGTTGGTTGTC TCTGTAGTCACACCTGGATGTAACCAGCTTCCTACTTTGGAGTCAGTGGAGAATTATACC CTGACCATAAATGATGACCAGTGTTTACTCCTCTCTGAGACTGTCTGGGGAGCTCTCCGA GGTCTGGAGACTTTTAGCCAGCTTGTTTGGAAATCTGCTGAGGGCACATTCTTTATCAAC AAGACTGAGATTGAGGACTTTCCCCGCTTTCCTCACCGGGGGCTTGCTGTTGGATACATC TCGCCATTACCTGCCACTCTCTAGCATCCTGGACACTCTGGATGTCATGGCGTACAAAAA TTGGAACGTGTTCCACTGGCATCTGGGTAGATGATCCTTCCTTCCCATATGAGAGCTTCA CTNTTCCAGAGCTATGAGAAAAAGGGGTCCTACACCCTGTCACCCACATCTACACAGACA GGATGGGAAAGGGAGGTCATTTGATACGCACGCTNCCGGGGAATCCCGGGTGCTTGGCAA AGTTTGACACTCCTGGCCCACTTTGTCCTGGGGACCAGNATCCCTGGATACTGACTCCTG CTACTCGGGGTCGGAA >OriGene 3' read for NM_000520 unedited
GCCTACGTGNACCGAGCCGCNATCNAGGATCGAGTAATATATTCTTTTTTTTTTATATAG AAAATGCCACATTACTCTTTATTGAATGCGAGCGCCAGCACCGGCCCCTTTCTCTCCAAG CACAGGGGCACGCAGGCAAGGGGCACGAAGGCAAGGGGCTCCGTCCCCTGGCCAGGATGC AGTGGAAGCCTGGCTCCACTACCATTCACCTACAGTCAGCTCCCTCCTCGGTGCCTGGGG CTCAGGTCTGTTCAAACTCCTGCTCACAGAAGCCTACATTGAGGGGTTGGGCCTGGACAC CTCGCCTCAGCAACTCACAGCGGAAGTGTGACAAACGTTCATAGGCAAATGTCAGGTCAG ATGTCAACTTGTTGCTCCACAGCCTTTCGGCAACAGCCCCTGCTCTGGGCCAGAGCCTGG GGACCAGGTTTGTGTTGTCCACATATTCTCCCCACATACAAGCCTCTCCACCAATCACCA GAGCCTTCTGCTCAGGGGTACCTTCAAATGCCAGGGGTTCCACTACGTAGAAATCCTTCC AGTCAGGGCCATAGGATATACGGTTCAGGTACCAGGGGGCAGAGAGAATGGCCCGGGAGC CGGCCTTGGTGACCAGTTCCAGCTCCTTCATATAGTTCACTGGAATATCCTCTCCCCCCC CCCTGTTGATTGTGTCTGGCTGAATCTTTACTTTTTTATCAAACACCTTCTGCCCNCCCC ATAGCCCTTGGCTTCCGCATAGACTATGTCCCTCTGCGTCCGGTTGTTGTAGGCCCTCCC CTTTCTTGGAGTCCTCCCCGAAGCCCTTTCTTCCTTTTCAAGTCTCTGGTCCTTGGGTTG GCGCTTCCCGCGGGGGGTTCCCACCCTTTTTTTCCCCGCTCGCNCTCTTTCTCTTGGGCC TCTCCTCTTCCTTCTCCGAATAAAGCTATATAAAAATAAAAGAAAA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_000520 |
| Insert Size | 1880 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000520.2, NP_000511.1 |
| RefSeq Size | 2255 bp |
| RefSeq ORF | 1590 bp |
| Locus ID | 3073 |
| UniProt ID | P06865 |
| Domains | Glyco_hydro_20 |
| Protein Families | Druggable Genome |
| Protein Pathways | Amino sugar and nucleotide sugar metabolism, Glycosaminoglycan degradation, Glycosphingolipid biosynthesis - ganglio series, Glycosphingolipid biosynthesis - globo series, Lysosome, Metabolic pathways, Other glycan degradation |
| Gene Summary | This gene encodes a member of the glycosyl hydrolase 20 family of proteins. The encoded preproprotein is proteolytically processed to generate the alpha subunit of the lysosomal enzyme beta-hexosaminidase. This enzyme, together with the cofactor GM2 activator protein, catalyzes the degradation of the ganglioside GM2, and other molecules containing terminal N-acetyl hexosamines. Mutations in this gene lead to an accumulation of GM2 ganglioside in neurons, the underlying cause of neurodegenerative disorders termed the GM2 gangliosidoses, including Tay-Sachs disease (GM2-gangliosidosis type I). Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. [provided by RefSeq, Jan 2016] Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region compared to variant 1. The encoded isoform (2) is shorter than isoform 1. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203185 | HEXA (Myc-DDK-tagged)-Human hexosaminidase A (alpha polypeptide) (HEXA) |
CNY 3,936.00 |
|
| RC203185L1 | Lenti ORF clone of Human hexosaminidase A (alpha polypeptide) (HEXA), Myc-DDK-tagged |
CNY 6,336.00 |
|
| RC203185L2 | Lenti ORF clone of Human hexosaminidase A (alpha polypeptide) (HEXA), mGFP tagged |
CNY 5,890.00 |
|
| RC203185L3 | Lenti ORF clone of Human hexosaminidase A (alpha polypeptide) (HEXA), Myc-DDK-tagged |
CNY 6,336.00 |
|
| RC203185L4 | Lenti ORF clone of Human hexosaminidase A (alpha polypeptide) (HEXA), mGFP tagged |
CNY 6,336.00 |
|
| RG203185 | HEXA (tGFP-tagged) - Human hexosaminidase A (alpha polypeptide) (HEXA) |
CNY 5,536.00 |
|
| SC320889 | HEXA (untagged)-Human hexosaminidase A (alpha polypeptide) (HEXA) |
CNY 3,944.00 |
