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IL8 (CXCL8) (NM_000584) Human Untagged Clone
CAT＃: SC119807
IL8 (untagged)-Human interleukin 8 (IL8)
CNY 1,800.00
| Cited in 2 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | GCP-1; GCP1; IL8; LECT; LUCT; LYNAP; MDNCF; MONAP; NAF; NAP-1; NAP1; SCYB8 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_000584 edited
GAATTCGGCACGAGGCAGCAGAGCACACAAGCTTCTAGGACAAGAGCCAGGAAGAAACCA CCGGAAGGAACCATCTCACTGTGTGTAAACATGACTTCCAAGCTGGCCGTGGCTCTCTTG GCAGCCTTCCTGATTTCTGCAGCTCTGTGTGAAGGTGCAGTTTTGCCAAGGAGTGCTAAA GAACTTAGATGTCAGTGCATAAAGACATACTCCAAACCTTTCCACCCCAAATTTATCAAA GAACTGAGAGTGATTGAGAGTGGACCACACTGCGCCAACACAGAAATTATTGTAAAGCTT TCTGATGGAAGAGAGCTCTGTCTGGACCCCAAGGAAAACTGGGTGCAGAGGGTTGTGGAG AAGTTTTTGAAGAGGGCTGAGAATTCATAAAAAAATTCATTCTCTGTGGTATCCAAGAAT CAGTGAAGATGCCAGTGAAACTTCAAGCAAATCTACTTCAACACTTCATGTATTGTGTGG GTCTGTTGTAGGGTTGCCAGATGCAATACAAGATTCCTGGTTAAATTTGAATTTCAGTAA ACAATGAATAGTTTTTCATTGTACCATGAAATATCCAGAACATACTTATATGTAAAGTAT TATTTATTTGAATCTACAAAAAACAACAAATAATTTTTAAATATAAGGATTTTCCTAGAT ATTGCACGGGAGAATATACAAATAGCAAAATTGAGGCCAAGGGCCAAGAGAATATCCGAA CTTTAATTTCAGGAATTGAATGGGTTTGCTAGAATGTGATATTTGAAGCATCACATAAAA ATGATGGGACAATAAATTTTGCCATAAAGTCAAATTTAGCTGGAAATCCTGGATTTTTTT CTGTTAAATCTGGCAACCCTAGTCTGCTAGCCAGGATCCACAAGTCCTTGTTCCACTGTG CCTTGGTTTCTCCTTTATTTCTAAGTGGAAAAAGTATTAGCCACCATCTTACCTCACAGT GATGTTGTGAGGACATGTGGAAGCACTTTAAGTTTTTTCATCATAACATAAATTATTTTC AAGTGTAACTTATTAACCTATTTATTATTTATGTATTTATTTAAGCATCAAATATTTGTG CAAGAATTTGGAAAAATAGAAGATGAATCATTGATTGAATAGTTATAAAGATGTTATAGT AAATTTATTTTATTTTAGATATTAAATGATGTTTTATTAGATAAATTTCAATCAGGGTTT TTAGATTAAACAAACAAACAATTGGGTACCCAGTTAAATTTTCATTTCAGATATACAACA AATAATTTTTTAGTATAAGTACATTATTGTTTATCTGAAATTTTAATTGAACTAACAATC CTAGTTTGATACTCCCAGTCTTGTCATTGCCAGCTGTGTTGGTAGTGCTGTGTTGAATTA CGGAATAATGAGTTAGAACTATTAAAACAGCCAAAACTCCACAGTCAATATTAGTAATTT CTTGCTGGTTGAAACTTGTTTATTATGTACAAATAGATTCTTATAATATTATTTAAATGA CTGCATTTTTAAATACAAGGCTTTATATTTTTAACTTTAAGATGTTTTTATGTGCTCTCC AAATTTTTTTTACTGTTTCTGATTGTATGGAAATATAAAAGTAAATATGAAACATTTAAA ATATAATTTGTTGTCAAAGCAAAAAAAAAAAAAAAAAACTCGAC >OriGene 5' read for NM_000584 unedited
ACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCAGCAGAGCACACAAGCTTCTAGGA CAAGAGCCAGGAAGAAACCACCGGAAGGAACCATCTCACTGTGTGTAAACATGACTTCCA AGCTGGCCGTGGCTCTCTTGGCAGCCTTCCTGATTTCTGCAGCTCTGTGTGAAGGTGCAG TTTTGCCAAGGAGTGCTAAAGAACTTAGATGTCAGTGCATAAAGACATACTCCAAACCTT TCCACCCCAAATTTATCAAAGAACTGAGAGTGATTGAGAGTGGACCACACTGCGCCAACA CAGAAATTATTGTAAAGCTTTCTGATGGAAGAGAGCTCTGTCTGGACCCCAAGGAAAACT GGGTGCAGAGGGTTGTGGAGAAGTTTTTGAAGAGGGCTGAGAATTCATAAAAAAATTCAT TCTCTGTGGTATCCAAGAATCAGTGAAGATGCCAGTGAAACTTCAAGCAAATCTACTTCA ACACTTCATGTATTGTGTGGGTCTGTTGTAGGGTTGCCAGATGCAATACAAGATTCCTGG TTAAATTTGAATTTCAGTAAACAATGAATAGTTTTTCATTGTACCATGAAATATCCAGAA CATACTTATATGTAAAGTATTATNTATTTGAATCTACAAAAAACACANNATATTTTTAAA TATAAGGATTTTCCTAGATATTGCACGGGGAGATATACAAATAGCANAATTGAGGCCCAG GGNCCAGAGGATATCCCGAACTTTATTNCAGGAANTGAATGGNTTGCTAGAATGTGATAT TTGAAGCATACATANAATGATGGGACANTAAATTTGCCATAAGTCAAATAGCTGGAAATC TGNATTNTTTTCTGTAAATCTGCACCCNTATCTGCTAGCCCAGATCCACAGTCCTTGTCC ACTGGGCCNTGGTNCTCCTTTATNCTAGNGGAAAAGTATANCCACCATCTACTNACATNG ATGTGGGAAGACATGTGAAGCTTNTAGTTT >OriGene 3' read for NM_000584 unedited
ACCGCGGCCGCAATCNANAGTCGAGTTTTTTTTTTTTTTTTTTGCTTTGACAACAAATTA TATTTTAAATGTTTCATATTTACTTTTATATTTCCATACAATCAGAAACAGTAAAAAAAA TTTGGAGAGCACATAAAAACATCTTAAAGTTAAAAATATAAAGCCTTGTATTTAAAAATG CAGTCATTTAAATAATATTATAAGAATCTATTTGTACATAATAAACAAGTTTCAACCAGC AAGAAATTACTAATATTGACTGTGGAGTTTTGGCTGTTTTAATAGTTCTAACTCATTATT CCGTAATTCAACACAGCACTACCAACACAGCTGGCAATGACAAGACTGGGAGTATCAAAC TAGGATTGTTAGTTCAATTAAAATTTCAGATAAACAATAATGTACTTATACTAAAAAATT ATTTGTTGTATATCTGAAATGAAAATTTAACTGGGTACCCAATTGTTTGTTTGTTTAATC TAAAAACCCTGATTGAAATTTATCTAATAAAACATCATTTAATATCTAAAATAAAATAAA TTTACTATAACATCTTTATAACTATTCAATCAATGCATCATCTTCTATTTTTTCAAACTT CTTGCACAAATATTTGATGCCTAAATTAATCCCTAACTAATAAATTGGCTGATAAAGCTA CACCTTGAAAATAATTTATGTTATGGATGAAAAAACTTAAAGTGCTTACACCTGGGCTCA CCACATACCTGCGGAGGAAAAAGCGTGCCTAATACTTTTCCCCTTAGAAAAAAGGAAAAC CCGGGCTCATGGGAACAAGACCTGGGGTTCCTGGCTTACAACCCGGGTGGCCATATTACC GCACAACCCGGGATGCCAGCTTATTGGCCCTCGGACGAATTCTGCGCCCGTCTTTATGTG GGCCCGCGACCGCCTTCGCACGACCCTTGCCACCGTCTTTACTGCG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_000584 |
| Insert Size | 1690 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000584.2, NP_000575.1 |
| RefSeq Size | 1666 bp |
| RefSeq ORF | 300 bp |
| Locus ID | 3576 |
| UniProt ID | P10145 |
| Domains | IL8 |
| Protein Families | Druggable Genome, Secreted Protein, Transmembrane |
| Protein Pathways | Bladder cancer, Chemokine signaling pathway, Cytokine-cytokine receptor interaction, Epithelial cell signaling in Helicobacter pylori infection, NOD-like receptor signaling pathway, Pathways in cancer, RIG-I-like receptor signaling pathway, Toll-like receptor signaling pathway |
| Gene Summary | The protein encoded by this gene is a member of the CXC chemokine family and is a major mediator of the inflammatory response. The encoded protein is commonly referred to as interleukin-8 (IL-8). IL-8 is secreted by mononuclear macrophages, neutrophils, eosinophils, T lymphocytes, epithelial cells, and fibroblasts. It functions as a chemotactic factor by guiding the neutrophils to the site of infection. Bacterial and viral products rapidly induce IL-8 expression. IL-8 also participates with other cytokines in the proinflammatory signaling cascade and plays a role in systemic inflammatory response syndrome (SIRS). This gene is believed to play a role in the pathogenesis of the lower respiratory tract infection bronchiolitis, a common respiratory tract disease caused by the respiratory syncytial virus (RSV). The overproduction of this proinflammatory protein is thought to cause the lung inflammation associated with csytic fibrosis. This proinflammatory protein is also suspected of playing a role in coronary artery disease and endothelial dysfunction. This protein is also secreted by tumor cells and promotes tumor migration, invasion, angiogenesis and metastasis. This chemokine is also a potent angiogenic factor. The binding of IL-8 to one of its receptors (IL-8RB/CXCR2) increases the permeability of blood vessels and increasing levels of IL-8 are positively correlated with increased severity of multiple disease outcomes (eg, sepsis). This gene and other members of the CXC chemokine gene family form a gene cluster in a region of chromosome 4q. [provided by RefSeq, May 2020] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
The combined effects of physicochemical properties of size-fractionated ambient particulate matter on in vitro toxicity in human A549 lung epithelial cells
,Akhtar, US;Rastogi, N;McWhinney, RD;Urch, B;,
Toxicology Reports Jul 2014
[CXCL8]
|
|
Tristetraprolin regulates IL-8 mRNA stability in cystic fibrosis lung epithelial cells
,Nagaraja Sethuraman Balakathiresan, Sharmistha Bhattacharyya, Usha Gutti, Robert P Long, Catherine Jozwik, Wei Huang, Meera Srivastava, Harvey B. Pollard, and Roopa Biswas,
Am J Physiol Lung Cell Mol Physiol, Jun 2009; 296: L1012 - L1018.
[CXCL8]
|
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Resources
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