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ALAS1 (NM_000688) Human Untagged Clone
CAT＃: SC119768
ALAS1 (untagged)-Human aminolevulinate, delta-, synthase 1 (ALAS1), transcript variant 1
CNY 4,776.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ALAS; ALAS-H; ALAS3; ALASH; MIG4 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC119768 sequence for NM_000688 edited (data generated by NextGen Sequencing)
ATGGAGAGTGTTGTTCGCCGCTGCCCATTCTTATCCCGAGTCCCCCAGGCCTTTCTGCAG AAAGCAGGCAAATCTCTGTTGTTCTATGCCCAAAACTGCCCCAAGATGATGGAAGTTGGG GCCAAGCCAGCCCCTCGGGCATTGTCCACTGCAGCAGTACACTACCAACAGATCAAAGAA ACCCCTCCGGCCAGTGAGAAAGACAAAACTGCTAAGGCCAAGGTCCAACAGACTCCTGAT GGATCCCAGCAGAGTCCAGATGGCACACAGCTTCCGTCTGGACACCCCTTGCCTGCCACA AGCCAGGGCACTGCAAGCAAATGCCCTTTCCTGGCAGCACAGATGAATCAGAGAGGCAGC AGTGTCTTCTGCAAAGCCAGTCTTGAGCTTCAGGAGGATGTGCAGGAAATGAATGCCGTG AGGAAAGAGGTTGCTGAAACCTCAGCAGGCCCCAGTGTGGTTAGTGTGAAAACCGATGGA GGGGATCCCAGTGGACTGCTGAAGAACTTCCAGGACATCATGCAAAAGCAAAGACCAGAA AGAGTGTCTCATCTTCTTCAAGATAACTTGCCAAAATCTGTTTCCACTTTTCAGTATGAT CGTTTCTTTGAGAAAAAAATTGATGAGAAAAAGAATGACCACACCTATCGAGTTTTTAAA ACTGTGAACCGGCGAGCACACATCTTCCCCATGGCAGATGACTATTCAGACTCCCTCATC ACCAAAAAGCAAGTGTCAGTCTGGTGCAGTAATGACTACCTAGGAATGAGTCGCCACCCA CGGGTGTGTGGGGCAGTTATGGACACTTTGAAACAACATGGTGCTGGGGCAGGTGGTACT AGAAATATTTCTGGAACTAGTAAATTCCATGTGGACTTAGAGCGGGAGCTGGCAGACCTC CATGGGAAAGATGCCGCACTCTTGTTTTCCTCGTGCTTTGTGGCCAATGACTCAACCCTC TTCACCCTGGCTAAGATGATGCCAGGCTGTGAGATTTACTCTGATTCTGGGAACCATGCC TCCATGATCCAAGGGATTCGAAACAGCCGAGTGCCAAAGTACATCTTCCGCCACAATGAT GTCAGCCACCTCAGAGAACTGCTGCAAAGATCTGACCCCTCAGTCCCCAAGATTGTGGCA TTTGAAACTGTCCATTCAATGGATGGGGCGGTGTGCCCACTGGAAGAGCTGTGTGATGTG GCCCATGAGTTTGGAGCAATCACCTTCGTGGATGAGGTCCACGCAGTGGGGCTTTATGGG GCTCGAGGCGGAGGGATTGGGGATCGGGATGGAGTCATGCCAAAAATGGACATCATTTCT GGAACACTTGGCAAAGCCTTTGGTTGTGTTGGAGGGTACATCGCCAGCACGAGTTCTCTG ATTGACACCGTACGGTCCTATGCTGCTGGCTTCATCTTCACCACCTCTCTGCCACCCATG CTGCTGGCTGGAGCCCTGGAGTCTGTGCGGATCCTGAAGAGCGCTGAGGGACGGGTGCTT CGCCGCCAGCACCAGCGCAACGTCAAACTCATGAGACAGATGCTAATGGATGCCGGCCTC CCTGTTGTCCACTGCCCCAGCCACATCATCCCTGTGCGGGTTGCAGATGCTGCTAAAAAC ACAGAAGTCTGTGATGAACTAATGAGCAGACATAACATCTACGTGCAAGCAATCAATTAC CCTACGGTGCCCCGGGGAGAAGAGCTCCTACGGATTGCCCCCACCCCTCACCACACACCC CAGATGATGAACTACTTCCTTGAGAATCTGCTAGTCACATGGAAGCAAGTGGGGCTGGAA CTGAAGCCTCATTCCTCAGCTGAGTGCAACTTCTGCAGGAGGCCACTGCATTTTGAAGTG ATGAGTGAAAGAGAGAAGTCCTATTTCTCAGGCTTGAGCAAGTTGGTATCTGCTCAGGCC TGA Clone variation with respect to NM_000688.4 >OriGene 5' read for NM_000688 unedited
GTATTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGCGCGGCCTGAGGCT GCTCCCGGACAAGGGCAACGAGCGTTTCGTTTGGACTTCTCGACTTGAGTGCCCGCCTCC TTCGCCGCCGCCTCTGCAGTCCTCAGCGCAGTCTTTCCACAGGAGCCAGCATACTTCCTG AACATGGAGAGTGTTGTTCGCCGCTGCCCATTCTTATCCCGAGTCCCCCAGGCCTTTCTG CAGAAAGCAGGCAAATCTCTGTTGTTCTATGCCCAAAACTGCCCCAAGATGATGGAAGTT GGGGCCAAGCCAGCCCCTCGGGCATTGTCCACTGCAGCAGTACACTACCAACAGATCAAA GAAACCCCTCCGGCCAGTGAGAAAGACAAAACTGCTAAGGCCAAGGTCCAACAGACTCCT GATGGATCCCAGCAGAGTCCAGATGGCACACAGCTTCCGTCTGGACACCCCTTGCCTGCC ACAAGCCAGGGCACTGCAAGCAAATGCCCTTTCCTGGCAGCACAGATGAATCAGAGAGGC AGCAGTGTCTTCTGCAAAGCCAGTCTTGAGCTTCAGGAGGATGTGCAGGAAATGAATGCC GTGAGGAAAGAGGTTGCTGAAACCTCAGCAGGCCCCAGTGTGGTTAGTGTGAAAACCGAT GGAGGGGATCCCAGTGGACTGCTGAAGAACTTCCAGGACATCATGCAAAAGCANAGACCA GANAGAGTGTCTCATCTTCTTCAAGATACTTGCCAAATCTGTTTCCACTTTCAGTATGAT CGTTTCTTTGAGACAAAAATGATGAGAAAAAGATGACCCACCTATCGAGATCTTAAACTG TGACCTGGCGAGCCACATCTTCCCCTGCCAATGATATTCAGACTCCTCACACCAAAAGCA GTGTCAGTCTGTTGCAGTATGACTACCTAGAATGAGTCGCCACCCAGGGTGTGG >OriGene 3' read for NM_000688 unedited
CGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTATTTCCAGGACTATGTTTT TACTATAGATTAAAATTTAATATAACTTAATTCACATATAAAGACAACTCTGAAGACCAT CTGGATATGATAATGGCCTGGGGTTAAGTGAAATAATTGAGGTCATGCTCAGGCCTGAGC AGATACCAACTTGCTCAAGCCTGAGAAATAGGACTTCTCTCTTTCACTCATCACTTCAAA ATGCAGTGGCCTCCTGCAGAAGTTGCACTCAGCTGAGGAATGAGGCTTCAGTTCCAGCCC CACTTGCTTCCATGTGACTAGCAGATTCTCAAGGAAGTAGTTCATCATCTGGGGTGTGTG GTGAGGGGTGGGGGCAATCCGTAGGAGCTCTTCTCCCCGGGGCACCGTAGGGTAATTGAT TGCTTGCACGTAGATGTTATGTCTGCTCATTAGTTCATCACAGACTTCTGTGTTTTTAGC AGCATCTGCAACCCGCACAGGGATGATGTGGCTGGGGCAGTGGACAACAGGGAGGCCGGC ATCCATTAGCATCTGTCTCATGAGTTTGACGTTGCGCTGGTGCTGGCGGCGAAGCACCCG TCCCTCAGCGCTCTTCAGGATCCGCACAGACTCCAGGGCTCCAGCCAGCAGCATGGGTGG CAGAGAGGTGGTGAAGATGAAGCCAGCAGCATAGGACCGTACGGTGTCAATCAGAGAACT CGTGCTGGCGATGTACCCTCCACACACCAAGGCTNTTGCCAGTGTCCAGAATGATGTCCA TTTTTGCATGACTCATCCCGATCCCATCCCCTCGCTCGGCCCATAAGCCCACTGCGTGAC CTATCACGAGGTGANTGCTCAACTATGGCCAATACACAGTTTTCGGGGCCACCGCCTCTT GATGGACGTTCATGCCATCTGGACGAGGGCAACTTTCGAGTTTGAGGGT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_000688 |
| Insert Size | 2290 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000688.4, NP_000679.1 |
| RefSeq Size | 2407 bp |
| RefSeq ORF | 1923 bp |
| Locus ID | 211 |
| UniProt ID | P13196 |
| Domains | ALA_synthase, aminotran_1_2 |
| Protein Pathways | Glycine, serine and threonine metabolism, Metabolic pathways, Porphyrin and chlorophyll metabolism |
| Gene Summary | This gene encodes the mitochondrial enzyme which is catalyzes the rate-limiting step in heme (iron-protoporphyrin) biosynthesis. The enzyme encoded by this gene is the housekeeping enzyme; a separate gene encodes a form of the enzyme that is specific for erythroid tissue. The level of the mature encoded protein is regulated by heme: high levels of heme down-regulate the mature enzyme in mitochondria while low heme levels up-regulate. A pseudogene of this gene is located on chromosome 12. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2015] Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2, and 3 encode the same protein. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219938 | ALAS1 (Myc-DDK-tagged)-Human aminolevulinate, delta-, synthase 1 (ALAS1), transcript variant 1 |
CNY 4,768.00 |
|
| RC219938L1 | Lenti ORF clone of Human aminolevulinate, delta-, synthase 1 (ALAS1), transcript variant 1, Myc-DDK-tagged |
CNY 7,168.00 |
|
| RC219938L2 | Lenti ORF clone of Human aminolevulinate, delta-, synthase 1 (ALAS1), transcript variant 1, mGFP tagged |
CNY 6,460.00 |
|
| RC219938L3 | Lenti ORF clone of Human aminolevulinate, delta-, synthase 1 (ALAS1), transcript variant 1, Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC219938L4 | Lenti ORF clone of Human aminolevulinate, delta-, synthase 1 (ALAS1), transcript variant 1, mGFP tagged |
CNY 6,460.00 |
|
| RG219938 | ALAS1 (tGFP-tagged) - Human aminolevulinate, delta-, synthase 1 (ALAS1), transcript variant 1 |
CNY 6,368.00 |
