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CD3D (NM_000732) Human Untagged Clone
CAT＃: SC119732
CD3D (untagged)-Human CD3d molecule, delta (CD3-TCR complex) (CD3D), transcript variant 1
CNY 2,400.00
CNY 2,950.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CD3-DELTA; IMD19; T3D |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC119732 sequence for NM_000732 edited (data generated by NextGen Sequencing)
ATGGAACATAGCACGTTTCTCTCTGGCCTGGTACTGGCTACCCTTCTCTCGCAAGTGAGC CCCTTCAAGATACCTATAGAGGAACTTGAGGACAGAGTGTTTGTGAATTGCAATACCAGC ATCACATGGGTAGAGGGAACGGTGGGAACACTGCTCTCAGACATTACAAGACTGGACCTG GGAAAACGCATCCTGGACCCACGAGGAATATATAGGTGTAATGGGACAGATATATACAAG GACAAAGAATCTACCGTGCAAGTTCATTATCGAATGTGCCAGAGCTGTGTGGAGCTGGAT CCAGCCACCGTGGCTGGCATCATTGTCACTGATGTCATTGCCACTCTGCTCCTTGCTTTG GGAGTCTTCTGCTTTGCTGGACATGAGACTGGAAGGCTGTCTGGGGCTGCCGACACACAA GCTCTGTTGAGGAATGACCAGGTCTATCAGCCCCTCCGAGATCGAGATGATGCTCAGTAC AGCCACCTTGGAGGAAACTGGGCTCGGAACAAGTGA Clone variation with respect to NM_000732.4 >OriGene 5' read for NM_000732 unedited
NGGGGCGTTCCAAATATTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACCAGNAC TAGGGGAGGGCAGCTCTCACCCAGGCTGATAGTTCGGTGACCTGGCTTTATCTACTGGAT GAGTTCCGCTGGGAGATGGAACATAGCACGTTTCTCTCTGGCCTGGTACTGGCTACCCTT CTCTCGCAAGTGAGCCCCTTCAAGATACCTATAGAGGAACTTGAGGACAGAGTGTTTGTG AATTGCAATACCAGCATCACATGGGTAGAGGGAACGGTGGGAACACTGCTCTCAGACATT ACAAGACTGGACCTGGGAAAACGCATCCTGGACCCACGAGGAATATATAGGTGTAATGGG ACAGATATATACAAGGACAAAGAATCTACCGTGCAAGTTCATTATCGAATGTGCCAGAGC TGTGTGGAGCTGGATCCAGCCACCGTGGCTGGCATCATTGTCACTGATGTCATTGCCACT CTGCTCCTTGCTTTGGGAGTCTTCTGCTTTGCTGGACATGAGACTGGAAGGCTGTCTGGG GCTGCCGACACACAAGCTCTGTTGAGGAATGACCAGGTCTATCAGCCCCTCCGAGATCGA GATGATGCTCAGTACAGCCACCTTGGAGGAAACTGGGCTCGGAACAAGTGAACCTGAGAC TGGTGGCTTCTAGAAGCAGCCATTACCAACTGTACCTTCCCTTCTTGCTCAGCCAATAAA TATATCCTCTTTCACTCAGAAAAAAAAAAAAAAAAAAAAAAACTCGACTCTAGATTGCGG CCGCGGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCATCCCTGTGACCCCTCCCCAG TGCCTCTCCTGGCCCTGGNGAGTGCACTCCAGTGCCCACCAGCCTGTCCTAATNAATAAG T >OriGene 3' read for NM_000732 unedited
NGGTACATCTATGNACCGCGCCGCAATCTANATCGAGTTTTTTTTTTTTTTTTTTTTTTT TCTGAGTGAAAGAGGATATATTTATTGGCTGAGCAAGAAGGGAAGGTACAGTTGGTAATG GCTGCTTCTAGAAGCCACCAGTCTCAGGTTCACTTGTTCCGAGCCCAGTTTCCTCCAAGG TGGCTGTACTGAGCATCATCTCGATCTCGGAGGGGCTGATAGACCTGGTCATTCCTCAAC AGAGCTTGTGTGTCGGCAGCCCCAGACAGCCTTCCAGTCTCATGTCCAGCAAAGCAGAAG ACTCCCAAAGCAAGGAGCAGAGTGGCAATGACATCAGTGACAATGATGCCAGCCACGGTG GCTGGATCCAGCTCCACACAGCTCTGGCACATTCGATAATGAACTTGCACGGTAGATTCT TTGTCCTTGTATATATCTGTCCCATTACACCTATATATTCCTCGTGGGTCCAGGATGCGT TTTCCCAGGTCCAGTCTTGTAATGTCTGAGAGCAGTGTTCCCACCGTTCCCTCTACCCAT GTGATGCTGGTATTGCAATTCACAAACACTCTGTCCTCAAGTTCCTCTATAGGTATCTTG GAGGGGCTCACTTGCGAGAGAAAGGTACCCAGTCCCAGGCCAGAGAAAACGTGCTATGTT CCATCTCCCAACGGAACTCATCCAGTAGATAAAGCCAGGTCCCCGACTATCAGCCTGGGG GAGAACTGTCCTCCCCTAACTCTGGTGCCGAATTCTCGGCCCGCCCTATGTGGGGGCGTA TTACAAAATTTTGAGGGGTTACTAAACGAACTTTGTTTTATAAAACCTCCCACCTACACC CCTCCCGCCCTTTTTGTCACCGGGGGGGGGTTTTACCACATTTTGGAAAGCCC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_000732 |
| Insert Size | 800 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000732.3, NP_000723.1 |
| RefSeq Size | 1585 bp |
| RefSeq ORF | 516 bp |
| Locus ID | 915 |
| UniProt ID | P04234 |
| Domains | ITAM |
| Protein Families | Druggable Genome |
| Protein Pathways | Hematopoietic cell lineage, Primary immunodeficiency, T cell receptor signaling pathway |
| Gene Summary | The protein encoded by this gene is part of the T-cell receptor/CD3 complex (TCR/CD3 complex) and is involved in T-cell development and signal transduction. The encoded membrane protein represents the delta subunit of the CD3 complex, and along with four other CD3 subunits, binds either TCR alpha/beta or TCR gamma/delta to form the TCR/CD3 complex on the surface of T-cells. Defects in this gene are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (SCIDBNK). Two transcript variants encoding different isoforms have been found for this gene. Other variants may also exist, but the full-length natures of their transcripts has yet to be defined. [provided by RefSeq, Feb 2009] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (A). |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Retroviral vectors for homologous recombination provide efficient cloning and expression in mammalian cells
,Kobayashi, E;Kishi, H;Ozawa, T;Horii, M;Hamana, H;Nagai, T;Muraguchi, A;,
Biochem. Biophys. Res. Commun., Feb 2014.
,PubMed ID 24462869
[CD3D]
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Other Versions
| SKU | Description | Size | Price |
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| RC210010 | CD3D (Myc-DDK-tagged)-Human CD3d molecule, delta (CD3-TCR complex) (CD3D), transcript variant 1 |
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| RC210010L1 | Lenti ORF clone of Human CD3d molecule, delta (CD3-TCR complex) (CD3D), transcript variant 1, Myc-DDK-tagged |
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| RC210010L2 | Lenti ORF clone of Human CD3d molecule, delta (CD3-TCR complex) (CD3D), transcript variant 1, mGFP tagged |
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| RC210010L3 | Lenti ORF clone of Human CD3d molecule, delta (CD3-TCR complex) (CD3D), transcript variant 1, Myc-DDK-tagged |
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| RC210010L4 | Lenti ORF clone of Human CD3d molecule, delta (CD3-TCR complex) (CD3D), transcript variant 1, mGFP tagged |
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| RG210010 | CD3D (tGFP-tagged) - Human CD3d molecule, delta (CD3-TCR complex) (CD3D), transcript variant 1 |
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