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BCKDHA (NM_000709) Human Untagged Clone
CAT＃: SC119718
BCKDHA (untagged)-Human branched chain keto acid dehydrogenase E1, alpha polypeptide (BCKDHA), nuclear gene encoding mitochondrial protein, transcript variant 1
CNY 6,664.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | BCKDE1A; MSU; MSUD1; OVD1A |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_000709, the custom clone sequence may differ by one or more nucleotides
ATGGCGGTAGCGATCGCTGCAGCGAGGGTCTGGCGGCTAAACCGTGGTTTGAGCCAGGCTGCCCTCCTGC TGCTGCGGCAGCCTGGGGCTCGGGGACTGGCTAGATCTCACCCCCCCAGGCAGCAGCAGCAGTTTTCATC TCTGGATGACAAGCCCCAGTTCCCAGGGGCCTCGGCGGAGTTTATAGATAAGTTGGAATTCATCCAGCCC AACGTCATCTCTGGAATCCCCATCTACCGCGTCATGGACCGGCAAGGCCAGATCATCAACCCCAGCGAGG ACCCCCACCTGCCGAAGGAGAAGGTGCTGAAGCTCTACAAGAGCATGACACTGCTTAACACCATGGACCG CATCCTCTATGAGTCTCAGCGGCAGGGCCGGATCTCCTTCTACATGACCAACTATGGTGAGGAGGGCACG CACGTGGGGAGTGCCGCCGCCCTGGACAACACGGACCTGGTGTTTGGCCAGTACCGGGAGGCAGGTGTGC TGATGTATCGGGACTACCCCCTGGAACTATTCATGGCCCAGTGCTATGGCAACATCAGTGACTTGGGCAA GGGGCGCCAGATGCCTGTCCACTACGGCTGCAAGGAACGCCACTTCGTCACTATCTCCTCTCCACTGGCC ACGCAGATCCCTCAGGCGGTGGGGGCGGCGTACGCAGCCAAGCGGGCCAATGCCAACAGGGTCGTCATCT GTTACTTCGGCGAGGGGGCAGCCAGTGAGGGGGACGCCCATGCCGGCTTCAACTTCGCTGCCACACTTGA GTGCCCCATCATCTTCTTCTGCCGGAACAATGGCTACGCCATCTCCACGCCCACCTCTGAGCAGTATCGC GGCGATGGCATTGCAGCACGAGGCCCCGGGTATGGCATCATGTCAATCCGCGTGGATGGTAATGATGTGT TTGCCGTATACAACGCCACAAAGGAGGCCCGACGGCGGGCTGTGGCAGAGAACCAGCCCTTCCTCATCGA GGCCATGACCTACAGGATCGGGCACCACAGCACCAGTGACGACAGTTCAGCGTACCGCTCGGTGGATGAG GTCAATTACTGGGATAAACAGGACCACCCCATCTCCCGGCTGCGGCACTATCTGCTGAGCCAAGGCTGGT GGGATGAGGAGCAGGAGAAGGCCTGGAGGAAGCAGTCCCGCAGGAAGGTGATGGAGGCCTTTGAGCAGGC CGAGCGGAAGCCCAAACCCAACCCCAACCTACTCTTCTCAGACGTGTATCAGGAGATGCCCGCCCAGCTC CGCAAGCAGCAGGAGTCTCTGGCCCGCCACCTGCAGACCTACGGGGAGCACTACCCACTGGATCACTTCG ATAAGTGA >OriGene 5' read for NM_000709 unedited
AATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTAGCGATCGCTGCAGCGA GGGTCTGGCGGCTAAACCGTGGTTTGAGCCAGGCTGCCCTCCTGCTGCTGCGGCAGCCTG GGGCTCGGGGACTGGCTAGATCTCACCCCCCCAGGCAGCAGCAGCAGTTTTCATCTCTGG ATGACAAGCCCCAGTTCCCAGGGGCCTCGGCGGAGTTTATAGATAAGTTGGAATTCATCC AGCCCAACGTCATCTCTGGAATCCCCATCTACCGCGTCATGGACCGGCAAGGCCAGATCA TCAACCCCAGCGAGGACCCCCACCTGCCGAAGGAGAAGGTGCTGAAGCTCTACAAGAGCA TGACACTGCTTAACACCATGGACCGCATCCTCTATGAGTCTCAGCGGCAGGGCCGGATCT CCTTCTACATGACCAACTATGGTGAGGAGGGCACGCACGTGGGGAGTGCCGCCGCCCTGG ACAACACGGACCTGGTGTTTGGCCAGTACCGGGAGGCAGGTGTGCTGATGTATCGGGACT ACCCCCTGGAACTATTCATGGCCCAGTGCTATGGCAACATCAGTGACTTGGGCAAGGGGC GCCAGATGCCTGTCCACTACGGCTGNCAGGAACGCCACTTCGTCACTATCTCCTCTCCAC TGGCCACGCAGATCCCTCAGGCGGGTGGGGGCGGCGTACGCAGCCAAGCGGGCCAATGCC AACAGGGNTCGTCATCTGGTACTTCNGCGAGGGGGCAGCCAGTGAGGGGGACGCCATGCC NGCTTNCACTTCGCTGCCACACTTGAGTGCCCCATCATCTTCTTCTTGCCGGACAAGGGN TACGCCATCTCCACGCCCACCTCTGAGCAGTATCGCGGCGATGGCATTGCAGCACGAGGC CCCCGGTATGGCATCATGTCATCCGCGTGGATG >OriGene 3' read for NM_000709 unedited
TGACCGCGGCCGCTTTTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTAAAAGCCAGCGCA GAGAGCAGTTTATTCGTGACCTCTGCTGGCCACTCGCACCTGCGCAAGTTCCACAGGCTG ACCTGCCATGTCCACCCCATGCCCAGAGGGGCTTCTAGTCCTGAATGTGCCCTCACCCAG CCCCTGGGAGAAGGCACTGTAACAACAGGTGAAGAGGGGGCTGACGGAGCCACAGCAACT GCTGCAGATGCTGTCCCCTGACAATGTAACAGCCGGGAGGAGCAGGGGTGAAGAGTGGCA GCCGCCCTGGCCGCTGAGTATTTTAGAGGGAGCTGACTGGGGAAGACAGTGGTGTGCTGT CAGGTCCCCCTGCTCCCCTTAGAGTGGGGCTACCTCTCGGGGTAGCTGAGGATGGGTGGG GGTGGGCTGAGCAGGTCTCACTTATCGAAGTGATCCAGTGGGTAGTGCTCCCCGTAGGTC TGCAGGTGGCGGGCCAGAGACTCCTGCTGCTTGCGGAGCTGGGCGGGCATCTCCTGATAC ACGTCTGAGAAGAGCAGGTTGGGGTTGGGTTTGGGCTTCCGCTCGGCCTGCTCAAAGGCC TCCATCACCTTCCTGCGGGACTGCTTCCTCCAGGCCTTCTCCTGCTCCTCATCCCACCAG NCCTTGGCTCACCAGATAGTGCCGCAGCCGGGAGATGGGGTGGCCCTGTTTATCCCATTA TTTGACCTCATCCACCGAGCGGTACGCTGAACTGCCGTCACTGGTGCTGCGGTGCCCGAT CCCTGTAGTCATGCCTCGAGACAAAGGTCTGGTTTCTGCCCAGCCCGCCGTGGGCCTCTT TGGGGGCTGTTACCGGAAAACCTCTTACCTCCCCCGGATTGACTGATGCCTACCCGGGGC TCGGCT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_000709 |
| Insert Size | 1870 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000709.2, NP_000700.1 |
| RefSeq Size | 1791 bp |
| RefSeq ORF | 1791 bp |
| Locus ID | 593 |
| UniProt ID | P12694 |
| Protein Families | Druggable Genome |
| Protein Pathways | Metabolic pathways, Valine, leucine and isoleucine degradation |
| Gene Summary | The branched-chain alpha-keto acid (BCAA) dehydrogenase (BCKD) complex is an innter mitochondrial enzyme complex that catalyzes the second major step in the catabolism of the branched-chain amino acids leucine, isoleucine, and valine. The BCKD complex consists of three catalytic components: a heterotetrameric (alpha2-beta2) branched-chain alpha-keto acid decarboxylase (E1), a dihydrolipoyl transacylase (E2), and a dihydrolipoamide dehydrogenase (E3). This gene encodes the alpha subunit of the decarboxylase (E1) component. Mutations in this gene result in maple syrup urine disease, type IA. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204816 | BCKDHA (Myc-DDK-tagged)-Human branched chain keto acid dehydrogenase E1, alpha polypeptide (BCKDHA), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 5,488.00 |
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| RC204816L1 | Lenti-ORF clone of BCKDHA (Myc-DDK-tagged)-Human branched chain keto acid dehydrogenase E1, alpha polypeptide (BCKDHA), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 7,888.00 |
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| RC204816L2 | Lenti-ORF clone of BCKDHA (mGFP-tagged)-Human branched chain keto acid dehydrogenase E1, alpha polypeptide (BCKDHA), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 5,890.00 |
|
| RC204816L3 | Lenti-ORF clone of BCKDHA (Myc-DDK-tagged)-Human branched chain keto acid dehydrogenase E1, alpha polypeptide (BCKDHA), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 5,890.00 |
|
| RC204816L4 | Lenti-ORF clone of BCKDHA (mGFP-tagged)-Human branched chain keto acid dehydrogenase E1, alpha polypeptide (BCKDHA), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 5,890.00 |
|
| RG204816 | BCKDHA (tGFP-tagged) - Human branched chain keto acid dehydrogenase E1, alpha polypeptide (BCKDHA), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 4,370.00 |
