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GAD67 (GAD1) (NM_000817) Human Untagged Clone
CAT＃: SC119631
GAD1 (untagged)-Human glutamate decarboxylase 1 (brain, 67kDa) (GAD1), transcript variant GAD67
CNY 6,648.00
| Cited in 1 publication. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CPSQ1; DEE89; GAD; SCP |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC119631 sequence for NM_000817 edited (data generated by NextGen Sequencing)
ATGGCGTCTTCGACCCCATCTTCGTCCGCAACCTCCTCGAACGCGGGAGCGGACCCCAAT ACCACTAACCTGCGCCCCACAACGTACGATACCTGGTGCGGCGTGGCCCACGGATGCACC AGAAAACTGGGGCTCAAGATCTGCGGCTTCTTGCAAAGGACCAACAGCCTGGAAGAGAAG AGTCGCCTTGTGAGTGCCTTCAAGGAGAGGCAATCCTCCAAGAACCTGCTTTCCTGTGAA AACAGCGACCGGGATGCCCGCTTCCGGCGCACAGAGACTGACTTCTCTAATCTGTTTGCT AGAGATCTGCTTCCGGCTAAGAACGGTGAGGAGCAAACCGTGCAATTCCTCCTGGAAGTG GTGGACATACTCCTCAACTATGTCCGCAAGACATTTGATCGCTCCACCAAGGTGCTGGAC TTTCATCACCCACACCAGTTGCTGGAAGGCATGGAGGGCTTCAACTTGGAGCTCTCTGAC CACCCCGAGTCCCTGGAGCAGATCCTGGTTGACTGCAGAGACACCTTGAAGTATGGGGTT CGCACAGGTCATCCTCGATTTTTCAACCAGCTCTCCACTGGATTGGATATTATTGGCCTA GCTGGAGAATGGCTGACATCAACGGCCAATACCAACATGTTTACATATGAAATTGCACCA GTGTTTGTCCTCATGGAACAAATAACACTTAAGAAGATGAGAGAGATAGTTGGATGGTCA AGTAAAGATGGTGATGGGATATTTTCTCCTGGGGGCGCCATATCCAACATGTACAGCATC ATGGCTGCTCGCTACAAGTACTTCCCGGAAGTTAAGACAAAGGGCATGGCGGCTGTGCCT AAACTGGTCCTCTTCACCTCAGAACAGAGTCACTATTCCATAAAGAAAGCTGGGGCTGCA CTTGGCTTTGGAACTGACAATGTGATTTTGATAAAGTGCAATGAAAGGGGGAAAATAATT CCAGCTGATTTTGAGGCAAAAATTCTTGAAGCCAAACAGAAGGGATATGTTCCCTTTTAT GTCAATGCAACTGCTGGCACGACTGTTTATGGAGCTTTTGATCCGATACAAGAGATTGCA GATATATGTGAGAAATATAACCTTTGGTTGCATGTCGATGCTGCCTGGGGAGGTGGGCTG CTCATGTCCAGGAAGCACCGCCATAAACTCAACGGCATAGAAAGGGCCAACTCAGTCACC TGGAACCCTCACAAGATGATGGGCGTGCTGTTGCAGTGCTCTGCCATTCTCGTCAAGGAA AAGGGTATACTCCAAGGATGCAACCAGATGTGTGCAGGATACCTCTTCCAGCCAGACAAG CAGTATGATGTCTCCTACGACACCGGGGACAAGGCAATTCAGTGTGGCCGCCACGTGGAT ATCTTCAAGTTCTGGCTGATGTGGAAAGCAAAGGGCACAGTGGGATTTGAAAACCAGATC AACAAATGCCTGGAACTGGCTGAATACCTCTATGCCAAGATTAAAAACAGAGAAGAATTT GAGATGGTTTTCAATGGCGAGCCTGAGCACACAAACGTCTGTTTTTGGTATATTCCACAA AGCCTCAGGGGTGTGCCAGACAGCCCTCAACGACGGGAAAAGCTACACAAGGTGGCTCCA AAAATCAAAGCCCTGATGATGGAGTCAGGTACGACCATGGTTGGCTACCAGCCCCAAGGG GACAAGGCCAACTTCTTCCGGATGGTCATCTCCAACCCAGCCGCTACCCAGTCTGACATT GACTTCCTCATTGAGGAGATAGAAAGACTGGGCCAGGATCTGTAA Clone variation with respect to NM_000817.2 111 t=>c >OriGene 5' read for NM_000817 unedited
GCTCTTGCCCGCCCGTCGCCTCTAAGGGCGGTAGGCGTGTACGGTGGGNAGGTCTATATA AGCAGAGCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCG CGAATTCGGCACGAGGCGCGTGCGAGAGGGAACTAGCGAGAACGAGGAAGCAGCTGGAGG TGACGCCGGGCAGATTACGCCTGTCAGGGCCGAGCCGAGCGGATCGCTGGGCGCTGTGCA GAGGAAAGGCGGGAGTGCCCGGCTCGCTGTCGCAGAGCCGAGCCTGTTTCTGCGCCGGAC CAGTCGAGGACTCTGGACAGTAGAGGCCCCGGGACGACCGAGCTGATGGCGTCTTCGACC CCATCTTCGTCCGCAACCTCCTCGAACGCGGGAGCGGACCCCAATACCACTAACCTGCGC CCCACAACGTACGATACCTGGTGCGGCGTGGCCCACGGATGCACCAGAAAACTGGGGCTC AAGATCTGCGGCTTCTTGCAAAGGACCAACAGCCTGGAAGAGAAGAGTCGCCTTGTGAGT GCCTTCAAGGAGAGGCAATCCTCCAAGAACCTGCTTTCCTGTGAAAACAGCGACCGGGAT GCCCGCTTCCGGCGCACAGAGACTGACTTCTCTAATCTGTTTGCTAGAGATCTGCTTCCG GCTAAGAACGGTGAGGAGCAAACCGTGCAATTCCTCCTGGAAGTGGTGGACATACTCCTC AACTATGTCCGCAAGACATTTGATCGCTCCACCAAGGTGCTGGACTTTCATCACCCACAC CAGTTGCTGGAAGGCATGGGAGGCTTCAACTTGGAGCTCTCTGACCACCCCGAGTCCCTG GAGCAGATCCTGGTTGACTGCAGAGACACCTTGAAGTATGGAGTTCGCACAGGTCATCCT CGATTTTTCAA >OriGene 3' read for NM_000817 unedited
CGGGCCGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTGTTCAAATTCTCCATGGAGA ATATTTTTATTTATTTATATTCTTCTTGCATACTCTAAAACGCACAGTGTGAATTTCCCT TCTTCAAAAACAGTTGTGAGCCTGGTCACTTTATCTGAGATCTTCAGAAGGTCTTCGGAA ATGTTGCCTTAGGTTTCAGCTAAGCGAGTCACAGAGATTGGTCATCAACTACTACAAAAT TACAGGAGATCTAGTTATTATCCAACAGTTGCTAGAAAAGCATTGAGAACACCAGACGTA CAGCCAGACAGAGAGCGTAAGAGAAGAGCAGATGCTATTTAAGTAACAGGATTTCCATAT ATATGACTTTATGTACAATCTTAGAATTCTCTTACAGACAGTAAAATGCTTAAATCATTA TTTTAAGGGCACTCTAATATAAATACAAATCTCACTAATTTTAATCTTTTCTCTTTGGCT CACAAATGCACGAAAATACCATAAACAGTTTTCAGAACTTACTTCATATATAAATAAGAT ACATTACAAATGGGAAGAAAATACAAGACGCCTCTTTAACATGGTACATCTCTGAATATA AATAAGCAAATATTTCATTGACCTACAATTAAACAATCATTGAAATCATGACACATAGTC TCCCCCTCCCACCACCAAGGTCAGACAACAGGAATTTACACAATCTACACAAATTAATCT CTAAAAATACAGCCATAGTACACCTGGGTACATGACTCTGCTACTATTTTCCCCTACAGG ATACTAATGGGGNAGGGNTGTGACTGTCATTGGNNGGNNGTTTTTCTCATCCATCATCAC TTCAGGTGGAGAAACGCTGACAGTGAGGTCGATTCTTACCCAACAGCATTCCTTTGGCCN NCTAGACATGGCGTTTGAAAAACAGTTNCCTTGGAGTGTTGACAANCTGGNTGGCNCATA GTTANCTCCTCCGTAC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_000817 |
| Insert Size | 3270 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000817.2, NP_000808.2 |
| RefSeq Size | 3488 bp |
| RefSeq ORF | 1785 bp |
| Locus ID | 2571 |
| UniProt ID | Q99259 |
| Domains | pyridoxal_deC |
| Protein Families | Druggable Genome |
| Protein Pathways | Alanine, aspartate and glutamate metabolism, beta-Alanine metabolism, Butanoate metabolism, Metabolic pathways, Taurine and hypotaurine metabolism, Type I diabetes mellitus |
| Gene Summary | This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form. [provided by RefSeq, Jul 2008] Transcript Variant: Transcript variant GAD67 represents the full-length and predominant form of this gene. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Antibodies to inhibitory synaptic proteins in neurological syndromes associated with glutamic Acid decarboxylase autoimmunity
,Gresa-Arribas, N;Ariño, H;Martínez-Hernández, E;Petit-Pedrol, M;Sabater, L;Saiz, A;Dalmau, J;Graus, F;,
PLoS ONE
,PubMed ID 25774787
[GAD1]
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207226 | GAD1 (Myc-DDK-tagged)-Human glutamate decarboxylase 1 (brain, 67kDa) (GAD1), transcript variant GAD67 |
CNY 6,632.00 |
|
| RC207226L1 | Lenti ORF clone of Human glutamate decarboxylase 1 (brain, 67kDa) (GAD1), transcript variant GAD67, Myc-DDK-tagged |
CNY 9,032.00 |
|
| RC207226L2 | Lenti ORF clone of Human glutamate decarboxylase 1 (brain, 67kDa) (GAD1), transcript variant GAD67, mGFP tagged |
CNY 5,890.00 |
|
| RC207226L3 | Lenti ORF clone of Human glutamate decarboxylase 1 (brain, 67kDa) (GAD1), transcript variant GAD67, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC207226L4 | Lenti ORF clone of Human glutamate decarboxylase 1 (brain, 67kDa) (GAD1), transcript variant GAD67, mGFP tagged |
CNY 5,890.00 |
|
| RG207226 | GAD1 (tGFP-tagged) - Human glutamate decarboxylase 1 (brain, 67kDa) (GAD1), transcript variant GAD67 |
CNY 8,232.00 |
