ABCF1 (NM_001090) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ABC27; ABC50
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001090, the custom clone sequence may differ by one or more nucleotides ATGCCGAAGGCGCCCAAGCAGCAGCCGCCGGAGCCCGAGTGGATCGGGGACGGAGAGAGCACGAGCCCAT CAGACAAAGTGGTGAAGAAAGGGAAGAAGGACAAGAAGATCAAAAAAACGTTCTTTGAAGAGCTGGCAGT AGAAGATAAACAGGCTGGGGAAGAAGAGAAAGTGCTCAAGGAGAAGGAGCAGCAGCAGCAGCAACAGCAA CAGCAGCAAAAAAAAAAGCGAGATACCCGAAAAGGCAGGCGGAAGAAGGATGTGGATGATGATGGAGAAG AGAAAGAGCTCATGGAGCGTCTTAAGAAGCTCTCAGTGCCAACCAGTGATGAGGAGGATGAAGTACCCGC CCCAAAACCCCGCGGAGGGAAGAAAACCAAGGGTGGTAATGTTTTTGCAGCCCTGATTCAGGATCAGAGT GAGGAAGAGGAGGAGGAAGAAAAACATCCTCCTAAGCCTGCCAAGCCGGAGAAGAATCGGATCAATAAGG CCGTATCTGAGGAACAGCAGCCTGCACTCAAGGGCAAAAAGGGAAAGGAAGAGAAGTCAAAAGGGAAGGC TAAGCCTCAAAATAAATTCGCTGCTCTGGACAATGAAGAGGAGGATAAAGAAGAAGAAATTATAAAGGAA AAGGAGCCTCCCAAACAAGGGAAGGAGAAGGCCAAGAAGGCAGAGCAGATGGAGTATGAGCGCCAAGTGG CTTCATTAAAAGCAGCCAATGCAGCTGAAAATGACTTCTCCGTGTCCCAGGCGGAGATGTCCTCCCGCCA AGCCATGTTAGAAAATGCATCTGACATCAAGCTGGAGAAGTTCAGCATCTCCGCTCATGGCAAGGAGCTG TTCGTCAATGCAGACCTGTACATTGTAGCCGGCCGCCGCTACGGGCTGGTAGGACCCAATGGCAAGGGCA AGACCACACTCCTCAAGCACATTGCCAACCGAGCCCTGAGCATCCCTCCCAACATTGATGTGTTGCTGTG TGAGCAGGAGGTGGTAGCAGATGAGACACCAGCAGTCCAGGCTGTTCTTCGAGCTGACACCAAGCGATTG AAGCTGCTGGAAGAGGAGCGGCGGCTTCAGGGACAGCTGGAACAAGGGGATGACACAGCTGCTGAGAGGC TAGAGAAGGTGTATGAGGAATTGCGGGCCACTGGGGCGGCAGCTGCAGAGGCCAAAGCACGGCGGATCCT GGCTGGCCTGGGCTTTGACCCTGAAATGCAGAATCGACCCACACAGAAGTTCTCAGGGGGCTGGCGCATG CGTGTCTCCCTGGCCAGGGCACTGTTCATGGAGCCCACACTGCTGATGCTGGATGAGCCCACCAACCACC TGGACCTCAACGCTGTCATCTGGCTTAATAACTACCTCCAGGGCTGGCGGAAGACCTTGCTGATCGTCTC CCATGACCAGGGCTTCTTGGATGATGTCTGCACTGATATCATCCACCTCGATGCCCAGCGGCTCCACTAC TATAGGGGCAATTACATGACCTTCAAAAAGATGTACCAGCAGAAGCAGAAAGAACTGCTGAAACAGTATG AGAAGCAAGAGAAAAAGCTGAAGGAGCTGAAGGCAGGCGGGAAGTCCACCAAGCAGGCGGAAAAACAAAC GAAGGAAGCCCTGACTCGGAAGCAGCAGAAATGCCGACGGAAAAACCAAGATGAGGAATCCCAGGAGGCC CCTGAGCTCCTGAAGCGCCCTAAGGAGTACACTGTGCGCTTCACTTTTCCAGACCCCCCACCACTCAGCC CTCCAGTGCTGGGTCTGCATGGTGTGACATTCGGCTACCAGGGACAGAAACCACTCTTTAAGAACTTGGA TTTTGGCATCGACATGGATTCAAGGATTTGCATTGTGGGCCCTAATGGTGTGGGGAAGAGTACGCTACTC CTGCTGCTGACTGGCAAGCTGACACCGACCCATGGGGAAATGAGAAAGAACCACCGGCTGAAAATTGGCT TCTTCAACCAGCAGTATGCAGAGCAGCTGCGCATGGAGGAGACGCCCACTGAGTACCTGCAGCGGGGCTT CAACCTGCCCTACCAGGATGCCCGCAAGTGCCTGGGCCGCTTCGGCCTGGAGAGTCACGCCCACACCATC CAGATCTGCAAACTCTCTGGTGGTCAGAAGGCGCGAGTTGTGTTTGCTGAGCTGGCCTGTCGGGAACCTG ATGTCCTCATCTTGGACGAGCCAACCAATAACCTGGACATAGAGTCTATTGATGCTCTAGGGGAGGCCAT CAATGAATACAAGGGTGCTGTGATCGTTGTCAGCCATGATGCCCGACTCATCACAGAAACCAATTGCCAG CTGTGGGTGGTGGAGGAGCAGAGTGTTAGCCAAATCGATGGTGACTTTGAAGACTACAAGCGGGAGGTGT TGGAGGCCCTGGGTGAAGTCATGGTCAGCCGGCCCCGAGAGTGA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_001090 unedited GATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGCCACAGTAG CTGTAACTGCCACCGCGATGCCGAAGGCGCCCAAGCAGCAGCCGCCGGAGCCCGAGTGGA TCGGGGACGGAGAGAGCACGAGCCCATCAGACAAAGTGGTGAAGAAAGGGAAGAAGGACA AGAAGATCAAAAAAACGTTCTTTGAAGAGCTGGCAGTAGAAGATAAACAGGCTGGGGAAG AAGAGAAAGTGCTCAAGGAGAAGGAGCAGCAGCAGCAGCAACAGCAACAGCAGCAAAAAA AAAAGCGAGATACCCGAAAAGGCAGGCGGAAGAAGGATGTGGATGATGATGGAGAAGAGA AAGAGCTCATGGAGCGTCTTAAGAAGCTCTCAGTGCCAACCAGTGATGAGGAGGATGAAG TACCCGCCCCAAAACCCCGCGGAGGGAAGAAAACCAAGGGTGGTAATGTTTTTGCAGCCC TGATTCAGGATCANAGTGAGGAAGAGGAGGAGGAAGAAAAACATCCTCCTAAGCCTGCCA AGCCGGAGAAGAATCGGATCAATAAGGCCGTATCTGAGGAACAGCAGCCTGCACTCAAGG GCAAAAAGGGAAAGGAAGAGAAGTCAAAAGGGAAGGCTAAGCCTCAAAATAAATTCGCTG CTCTGGACAATGAAGAGGAGGATAAAGAAGAAGAAATTATAAAGGAGAAGGGAGCCTCCC AAACAAGGGAAGGGAGAAAGCCAAGAAAGCAGAAGCAGGTTTCANAGGAAGAANGGAGAG GGGGGAAAAANAAGGAGGAGAANGGAGGAGAGTCTAAGGCANAATGATCCCTATGGCTCA TCTTANCAAAAAGGAGAAGAANAAGCTGGAAAACAGATGGATTATGAGCGCCAAG 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for NM_001090 unedited GCGGCCCGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTGGCAGCAACTCCTTTCCTT TATTTCTTCCCCTTGTAAAGGGAAATTCAAGTTCAGCAGCATTCCTTTCCTGCCCCAAGT CCTCAACCAGACAAGAGGCTGCAGGCACCAAATCTTGGGCTGGATAATGGCAAAGGCCTC AGAAGCTCACCTCCAGCTCTGAGCTTCAACAGCTGTTTGTACCAGTGAGTCAGCATTAAA TCCACCAGAAAAGAACAGCACCACCCAAAGACTGGGGGGCAGCTGGGCCTGAAGCTGTAG GGTAAATCAAAGGCAGGCTTCTGAGTGATGAGAGTCCTGAGACAATAGGCCACATAAACT TGGCTGGATGGAACCTCACAATAAGGTGGTCACCTCTTGTTTGTTTAGGGGGATGCCAAG GATAAGGCCAGCTCAGTTATATGAAGAGAAGCAGAACAAACAAGTCTTTCAGAGAAATGG ATGCAATCAGAGTGGGATCCCGGTCACATCAAGGTCACACTCCACCTTCATGTGCCTGAA TGGTTGCCAGGTCAGCTGCAGGCCANAGGCAGTCTTCAGAGGAGGGGAGACCACAGAGGA CTTCTAGGCCCCACAAATATGTCTCTCGGGAGACTTCTGGGAAGGAAAGCTTCACTCTCG GGGCCGGCTGACCCTGACTTCCCCCAAGGCCTNCAACACCTNCCGCTTGGAGCCTCAAAG CCCCCTCGATTTGGTAACCCTCTGTTCCCCCCACCCAAACTGCAATTGGGTTCTGGAATA ACCCGGCCTTATGGCTGCAACCATCACACACCCTTGTATCTTTGAGGCCCCCCTACACCT CAAAACCCCTTCTCCCAGCTACGGGTGCGCTCCCCAAAAGAGAGCCCAGTTCCCCCAAGG CACCTTCACAAACAAACTTCCCCCCTTGACCACCAACACGTCGCCAAA
Restriction Sites	NotI-NotI
ACCN	NM_001090
Insert Size	3270 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001090.2, NP_001081.1
RefSeq Size	3360 bp
RefSeq ORF	2424 bp
Locus ID	23
UniProt ID	Q8NE71
Domains	ABC_tran, AAA
Protein Families	Druggable Genome
Gene Summary	The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the GCN20 subfamily. Unlike other members of the superfamily, this protein lacks the transmembrane domains which are characteristic of most ABC transporters. This protein may be regulated by tumor necrosis factor-alpha and play a role in enhancement of protein synthesis and the inflammation process. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) lacks an alternate in-frame exon, compared to variant 1. The resulting protein (isoform b) is shorter than isoform a.

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