gamma Adaptin (AP1G1) (NM_001128) Human Untagged Clone

CAT#: SC119413

AP1G1 (untagged)-Human adaptor-related protein complex 1, gamma 1 subunit (AP1G1), transcript variant 2



  "NM_001128" in other vectors (4)

CNY 6,032.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


Anti-AP1G1 Rabbit Polyclonal Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms ADTG; CLAPG1
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC119413 sequence for NM_001128 edited (data generated by NextGen Sequencing)
ATGCCAGCCCCCATCAGATTGCGGGAGCTGATCCGGACCATCCGGACAGCCCGAACCCAA
GCTGAAGAACGAGAAATGATCCAGAAAGAATGTGCTGCAATCCGGTCATCTTTTAGAGAA
GAAGACAATACATACCGATGTCGGAATGTGGCAAAATTACTGTATATGCACATGCTGGGC
TACCCTGCTCACTTTGGACAGTTGGAGTGCCTCAAGCTTATTGCCTCTCAAAAATTTACA
GACAAACGCATTGGCTATTTAGGGGCAATGCTGCTGTTAGATGAAAGACAAGATGTCCAT
CTTCTCATGACCAACTGTATCAAGAATGATCTTAATCATAGCACGCAATTCGTACAGGGG
TTAGCACTTTGTACCCTCGGCTGCATGGGCTCCTCAGAGATGTGCAGAGATCTTGCAGGA
GAGGTAGAGAAGCTCCTGAAAACCTCCAACTCTTACTTAAGAAAAAAGGCAGCACTGTGT
GCTGTTCATGTCATCAGGAAAGTTCCTGAACTTATGGAGATGTTTTTACCAGCAACAAAA
AATTTATTGAATGAGAAGAACCATGGTGTCCTCCACACATCTGTAGTCCTCCTCACAGAA
ATGTGTGAGCGAAGCCCAGACATGCTTGCGCATTTCAGAAAGCTTGTGCCCCAATTAGTT
CGTATTTTAAAGAACCTCATCATGTCCGGATATTCACCAGAACATGATGTTTCTGGTATC
AGTGACCCCTTTTTGCAGGTACGAATTTTGCGGTTATTAAGAATTTTAGGACGAAATGAT
GATGATTCAAGTGAAGCTATGAATGATATATTAGCACAGGTTGCCACTAATACTGAGACT
AGTAAAAATGTAGGAAATGCTATTCTTTATGAAACGGTTTTGACTATCATGGATATTAAG
TCAGAGAGTGGATTGCGAGTCCTAGCCATAAATATCCTGGGTCGTTTCTTATTGAACAAT
GACAAGAATATTAGATATGTGGCTCTGACATCTTTGTTGAAGACTGTACAGACAGATCAT
AATGCAGTACAGAGGCACAGAAGCACAATTGTGGACTGTCTTAAAGATTTGGATGTCTCA
ATAAAACGGCGTGCAATGGAATTGAGTTTTGCCCTGGTAAATGGGAATAATATCCGAGGC
ATGATGAAAGAATTACTTTATTTTCTGGATTCGTGTGAGCCAGAATTTAAAGCAGACTGT
GCATCTGGAATCTTTCTTGCTGCAGAAAAGTATGCACCTTCCAAACGATGGCATATAGAC
ACAATTATGCGTGTTTTGACAACGGCAGGAAGTTATGTTCGTGATGATGCAGTCCCCAAT
TTAATCCAGTTAATAACTAATAGTGTGGAGATGCATGCCTATACTGTCCAGCGCCTGTAC
AAAGCAATTCTTGGTGATTATTCTCAACAACCTTTGGTACAAGTGGCTGCATGGTGTATA
GGTGAATATGGTGATCTTCTTGTATCTGGCCAGTGTGAAGAGGAAGAGCCTATTCAGGTA
ACAGAGGATGAAGTGTTGGATATTTTAGAAAGTGTCCTAATCTCTAATATGTCCACCTCT
GTGACACGAGGTTATGCCCTCACTGCCATTATGAAGCTTTCCACTCGATTCACTTGTACT
GTAAACCGAATTAAGAAAGTGGTTTCCATCTACGGAAGCAGCATTGATGTGGAACTCCAG
CAGAGGGCAGTAGAATATAATGCACTTTTCAAGAAATATGACCACATGAGGTCTGCCCTA
CTTGAGAGAATGCCTGTCATGGAAAAAGTGACCACAAATGGCCCTACTGAGATTGTGCAG
ACAAATGGAGAGACAGAACCAGCTCCACTAGAGACCAAACCGCCACCCTCTGGGCCACAG
CCCACCAGCCAGGCCAATGATTTATTGGATTTGTTGGGAGGAAATGACATAACACCTGTT
ATTCCAACTGCGCCTACAAGCAAACCATCTTCTGCTGGTGGAGAACTTCTTGATTTGCTG
GGAGACATCAACCTTACAGGTGCTCCAGCTGCTGCTCCTGCCCCTGCCTCAGTCCCACAG
ATATCCCAGCCCCCCTTCTTGTTGGATGGGCTTTCATCACAGCCTCTCTTCAATGATATT
GCTGCAGGCATCCCCTCCATCACAGCATACAGTAAGAATGGCTTGAAGATAGAATTCACC
TTTGAACGGTCAAATACCAACCCCAGTGTAACAGTGATAACGATACAGGCCTCCAACAGC
ACAGAGCTAGATATGACGGACTTTGTTTTCCAAGCTGCAGTACCAAAGACATTCCAGCTG
CAGCTCTTGTCTCCTAGCAGCAGCATTGTCCCAGCATTTAACACGGGGACCATCACACAA
GTCATTAAAGTTCTGAACCCTCAGAAGCAACAGCTGCGAATGCGGATCAAGCTTACATAT
AATCACAAGGGCTCAGCAATGCAAGATCTAGCAGAGGTGAACAACTTTCCCCCTCAGTCC
TGGCAATGA

Clone variation with respect to NM_001128.5
>OriGene 5' read for NM_001128 unedited
NGGTTCGAATATTTGTATACGACTCACTATTAGGGCGGCCGCGATTCGGCACGAGTGCCG
CCGCCCCCGGAGCTGCTGGTTTCATTCGAGGTTTCGGGCCGTTTCAGGGGTCCAGTCCCA
GGCTGTGGGTGTTGAGAAGAGAAGACAGAGAAGCTGGGCCAGGAGAGGCCGGGAGCCCCA
GGGGCTGCTCCGACTTCTGGAGGGCCGACCCCGAGGCCTCGGACCGGGAGGTTCCGCGCG
CCGCGGCGTTCCGTGTTGTGGCGTTGCCCCGCCGCTCCTGGCCCTGCGGACGTCAGCGTC
TAATAATTCTCCCGGTCTTCCTGATTGCCTGAAAGAGTCGGGACGGTTCGGTTGATTGTG
TTTCCCGGTTAGGACTCGGTGTAGGAGATCCATCGAACCACTCGCTCAGGGATGCCAGCC
CCCATCAGATTGCGGGAGCTGATCCGGACCATCCGGACAGCCCGAACCCAAGCTGAAGAA
CGAGAAATGATCCAGAAAGAATGTGCTGCAATCCGGTCATCTTTTAGAGAAGAAGACAAT
ACATACCGATGTCGGAATGTGGCAAAATTACTGTATATGCACATGCTGGGCTACCCTGCT
CACTTTGGANCAGTTGGAGTGCCTCAAGCTTATTGCCTCTCAAAAATTTACAGACAAACG
CATTGGCTATTTANGGGCAATGCTGCTGTTAGATGAAAGACNAGATGTCCATCTTCTCAT
GACCAACTGTATCAAGAATGATCTTAATCATAGCACGCAATTCGTACCGGGGGTTAGCAC
TTTGTACCCTCGGCTGCATGGGCTCTCAANAATGGGNCANAGATCTGCAGGGAGAGGTAN
AGAAGCTCCTGANACCTCCACTCTTACTTAGAAAAGCGCACTGGGGTGCTGGTATGCTCN
GGGGAAGTCCTGATATAGGC
>OriGene 3' read for NM_001128 unedited
ATTTTTAACCGTGTGGTCTTTTATTTAAGTGCCAGTGTTACAGTTACAACACAAATGTTC
CAGTTAGAAGGAATTCAAACGGAATGCCAAGGTCCAAGCCAGGCTCAAAAAATAAAAAGG
GAGGTTTGGAGTAATAAATAAAATGACTCCAATACTCACTCTTCCTAAGGGCAAAGGTCC
TTTTGATACAGAGTCTGATCTTTGAAACTGGTGAACTCCTCTTCCACCCATTACCATAGT
TCAAACAGGCAAGTTATGGGCTTAGGACCACTTTAAAATTTGGGGGGGGAATAGGGTCAT
TAATAACTATGAATATATCTTTTAAAAGGTGACCATTTTGCACTTTAAAGGGAATCAATT
TTGAAAATCATGGAAACTATTCATGACTACAGCTAAAAAATGGCGAAAAAGGGGAGCTGG
AAAAGCCTTGGAAGTTTCTATTACAAATAGAGCACCATATCCTTCATGCCAAATCTCAAC
AAAAGCTCTTTTTAACTCCATCTGTCCAGTGTTAACAAATAAACTCGCAAGGTCTGACCA
GTTCTTGGTAACAAACATACATGTGTGTGTCTGTGTGTATACAGCAATGCACAAAAAAGG
CTACCAGGAGCCTAATGCCTCTTTCAAACATTGGGGGAACCAGTAGAAAAAGGCAGGGCT
CCCTAATGTCCATTATTACATTTCCATTCCGAATGCCAGATGTTAAAAGTGCCTGAAGAT
GGTAACCCAGCTAGTGAGGAATAAATACCCCACCTTGCCCAGTCCACAGAGGAACAACAG
TNGAAAGAGGGGGCACTTCTTTGCTGCAAAGACAAATGAGTGTTTTTTCCCATGGATCGC
AGTCCTTTCCTCA
Restriction Sites NotI-NotI     
ACCN NM_001128
Insert Size 4280 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001128.5, NP_001119.3
RefSeq Size 6841 bp
RefSeq ORF 2469 bp
Locus ID 164
UniProt ID O43747
Domains Adaptin_N, Alpha_adaptinC2
Protein Pathways Lysosome
Gene Summary Adaptins are important components of clathrin-coated vesicles transporting ligand-receptor complexes from the plasma membrane or from the trans-Golgi network to lysosomes. The adaptin family of proteins is composed of four classes of molecules named alpha, beta-, beta prime- and gamma- adaptins. Adaptins, together with medium and small subunits, form a heterotetrameric complex called an adaptor, whose role is to promote the formation of clathrin-coated pits and vesicles. The protein encoded by this gene is a gamma-adaptin protein and it belongs to the adaptor complexes large subunits family. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Transcript Variant: This variant (2) lacks an in-frame exon compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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