DNase II (DNASE2) (NM_001375) Human Untagged Clone
CAT#: SC119284
DNASE2 (untagged)-Human deoxyribonuclease II, lysosomal (DNASE2)
CNY 3,656.00
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Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | DNASE2A; DNL; DNL2 |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC119284 sequence for NM_001375 edited (data generated by NextGen Sequencing)
ATGATCCCGCTGCTGCTGGCAGCGCTGCTGTGCGTCCCCGCCGGGGCCCTGACCTGCTAC GGGGACTCCGGGCAGCCTGTAGACTGGTTCGTGGTCTACAAGCTGCCAGCTCTTAGAGGG TCCGGGGAGGCGGCGCAGAGAGGGCTGCAGTACAAGTATCTGGACGAGAGCTCCGGAGGC TGGCGGGACGGCAGGGCACTCATCAACAGCCCGGAGGGGGCCGTGGGCCGAAGCCTGCAG CCGCTGTACCGGAGCAACACCAGCCAGCTCGCCTTCCTGCTCTACAATGACCAACCGCCT CAACCCAGCAAGGCTCAGGACTCTTCCATGCGTGGGCACACGAAGGGTGTCCTGCTCCTT GACCACGATGGGGGCTTCTGGCTGGTCCACAGTGTACCTAACTTCCCTCCACCGGCCTCC TCTGCTGCATACAGCTGGCCTCATAGCGCCTGTACCTACGGGCAGACCCTGCTCTGTGTG TCTTTTCCCTTCGCTCAGTTCTCGAAGATGGGCAAGCAGCTGACCTACACCTACCCCTGG GTCTATAACTACCAGCTGGAAGGGATCTTTGCCCAGGAATTCCCCGACTTGGAGAATGTG GTCAAGGGCCACCACGTTAGCCAAGAACCCTGGAACAGCAGCATCACACTCACATCCCAG GCCGGGGCTGTTTTCCAGAGCTTTGCCAAGTTCAGCAAATTTGGAGATGACCTGTACTCC GGCTGGTTGGCAGCAGCCCTTGGTACCAACCTGCAGGTCCAGTTCTGGCACAAAACTGTA GGCATCCTGCCCTCTAACTGCTCGGATATCTGGCAGGTTCTGAATGTGAACCAGATAGCT TTCCCTGGACCAGCCGGCCCAAGCTTCAACAGCACAGAGGACCACTCCAAATGGTGCGTG TCCCCAAAAGGGCCCTGGACCTGCGTGGGTGACATGAATCGGAACCAGGGAGAGGAGCAA CGGGGTGGGGGCACACTGTGTGCCCAGCTGCCAGCCCTCTGGAAAGCCTTCCAGCCGCTG GTGAAGAACTACCAGCCCTGTAATGGCATGGCCAGGAAGCCCAGCAGAGCTTATAAGATC TAA Clone variation with respect to NM_001375.2 >OriGene 5' read for NM_001375 unedited
TAGATTTGTATACGACTCACTATAGGGNNCGGCCGCGCAATTCGGCACGAGGATGTAACC CAGCGCCCCGCAGTCCCGACACAGATTCCTGGATCTCAGCCCCATAGCAGCTATGATCCC GCTGCTGCTGGCAGCGCTGCTGTGCGTCCCCGCCGGGGCCCTGACCTGCTACGGGGACTC CGGGCAGCCTGTAGACTGGTTCGTGGTCTACAAGCTGCCAGCTCTTAGAGGGTCCGGGGA GGCGGCGCAGAGAGGGCTGCAGTACAAGTATCTGGACGAGAGCTCCGGAGGCTGGCGGGA CGGCAGGGCACTCATCAACAGCCCGGAGGGGGCCGTGGGCCGAAGCCTGCAGCCGCTGTA CCGGAGCAACACCAGCCAGCTCGCCTTCCTGCTCTACAATGACCAACCGCCTCAACCCAG CAAGGCTCAGGACTCTTCCATGCGTGGGCACACGAAGGGTGTCCTGCTCCTTGACCACGA TGGGGGCTTCTGGCTGGTCCACAGTGTACCTAACTTCCCTCCACCGGCCTCCTCTGCTGC ATACAGCTGGCCTCATAGCGCCTGTACCTACGGGCAGACCCTGCTCTGTGTGTCTTTTCC CTTCGCTCAGTTCTCGAAGATGGGCAAGCAGCTGACCTACACCTACCCCTGGGTCTATAA CTACCAGCTGGAAGGGATCTTTGCCCAGGAATTCCCCGACTTGGAGAATGTGGTCAAGGG CCACCACGTTAGCCAAGAACCCTGGAACAGCAGCATCACACTCACATCCCAGCCCCGGGC TGTTTTCCAGAGCTCTGCCAAGTTCAGCAAATTTGGAGATGACCTGTACTCCGGCTGGTT GGCAGCAGCCCTTGGTCCAACCTGCAGGTCCAGTTCCTGCACAAAACTGTTAGCATCCTG CCCTCTAACTGCTCGNATATTTGGCAGGTTCTGAATGTGGACCCGCATACCTTCCCTGTA CCAN >OriGene 3' read for NM_001375 unedited
CTAGAGTCGAGTTTTTTTTTTTTTTTTTTAGATTTTTTATTTTTTATTTTTTTGACACAG AGTCTCATTCTGTCACCCAGGCTGAAGTGCCGTGGTGTTAGCTCAGCTCATGACAACCTC TGCCTCCCGAGTTCAAGCGATTCTCCTGCCTTACCCTCCCGAGGAGCTAGGACCACAGAC GCGTGCCACCATGCCTGGCTAATCCTTAAACATTCTTAATGGATATGGGGTTTCACCATG TTGGCCAGGCTGGCCTCGAACTCCTGACCTCAAGTGATCTGCCCATCTAAGACTCCCAAA GTGCTGGGGCTACAGATGTGAGCCACTGCACCCACCCGTACACCGACCACCCTTTTTTTT TGAAACAGAGCCTTGCTCTGTGACCTCACGCTGGCGCCCCACATAGCCCACTGTCACCAT GATTCCCACTGGCCCAACCCATCCTCTGGCTTCACTGCGCCGGTACCCACAGGCCTTTAC CACCCGCGCCTGCTTCCCTCTCTTCCAACTCCATCTCCACATGTGGCCTCCCTTTGCAAC CCCCGCCTGTCCCCCCATCCCCTGCCTTCTAAGTGCACCCTTACCTCCTTCGACTTCGCC AAGTCGCATGGAATACCATACCCGACCGCCTGCCCCTTGCCCCCAATGGCCTCCAACATT ACTAACCCCTGCTGGGCCTACCCGGCCCATTCCATCACAGGGCTTGCCAACTCCCCCAAC CCCCGTCCGTAACGATTATATTAAGGGCTCGCCCTCCGGGCCCCACAGTTGTGCCACACC TCCCCACCACTATACATTCTTGCCACACCCCCCTGCGACACCCCCCCGTCTCGCGGCGAC TTTTTCGGGACCCTCAACCCCTCTCTGCGTCGATTATACTCGCGCCTATATTAACCTTGG CTTCATTTGACCACACCGCGCAANTGTGCTTGCCATATTTATCACGCCTTCCGCCTTTCC CCTTCACCCCATTACGGCGACGCTCAATATAATACAGCGCTCATACACCCCGCATTCTCA TCGCTNGTCCCCATGCGCTGCCGCACATTACCGCCCGTCCCGTGCCTCCCACTCCTCTAG TCACGCCTCGCTCTANTCGCTNACCACACACCCACTCACGTTGTCGTG |
Restriction Sites | NotI-NotI |
ACCN | NM_001375 |
Insert Size | 1790 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_001375.2, NP_001366.1 |
RefSeq Size | 2011 bp |
RefSeq ORF | 1083 bp |
Locus ID | 1777 |
UniProt ID | O00115 |
Domains | DNase_II |
Protein Families | Druggable Genome |
Protein Pathways | Lysosome |
Gene Summary | This gene encodes a member of the DNase family. The protein, located in the lysosome, hydrolyzes DNA under acidic conditions and mediates the breakdown of DNA during erythropoiesis and apoptosis. Two codominant alleles have been characterized, DNASE2*L (low activity) and DNASE2*H (high activity), that differ at one nucleotide in the promoter region. The DNASE2*H allele is represented in this record. [provided by RefSeq, Jul 2008] |
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RC209573L2 | Lenti ORF clone of Human deoxyribonuclease II, lysosomal (DNASE2), mGFP tagged |
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RC209573L3 | Lenti ORF clone of Human deoxyribonuclease II, lysosomal (DNASE2), Myc-DDK-tagged |
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RC209573L4 | Lenti ORF clone of Human deoxyribonuclease II, lysosomal (DNASE2), mGFP tagged |
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RG209573 | DNASE2 (tGFP-tagged) - Human deoxyribonuclease II, lysosomal (DNASE2) |
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SC322699 | DNASE2 (untagged)-Human deoxyribonuclease II, lysosomal (DNASE2) |
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