DNase II (DNASE2) (NM_001375) Human Untagged Clone

CAT#: SC119284

DNASE2 (untagged)-Human deoxyribonuclease II, lysosomal (DNASE2)



  "NM_001375" in other vectors (7)

CNY 3,656.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
Rabbit Polyclonal DNase II Antibody
    • 100 ug

CNY 5,808.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms DNASE2A; DNL; DNL2
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC119284 sequence for NM_001375 edited (data generated by NextGen Sequencing)
ATGATCCCGCTGCTGCTGGCAGCGCTGCTGTGCGTCCCCGCCGGGGCCCTGACCTGCTAC
GGGGACTCCGGGCAGCCTGTAGACTGGTTCGTGGTCTACAAGCTGCCAGCTCTTAGAGGG
TCCGGGGAGGCGGCGCAGAGAGGGCTGCAGTACAAGTATCTGGACGAGAGCTCCGGAGGC
TGGCGGGACGGCAGGGCACTCATCAACAGCCCGGAGGGGGCCGTGGGCCGAAGCCTGCAG
CCGCTGTACCGGAGCAACACCAGCCAGCTCGCCTTCCTGCTCTACAATGACCAACCGCCT
CAACCCAGCAAGGCTCAGGACTCTTCCATGCGTGGGCACACGAAGGGTGTCCTGCTCCTT
GACCACGATGGGGGCTTCTGGCTGGTCCACAGTGTACCTAACTTCCCTCCACCGGCCTCC
TCTGCTGCATACAGCTGGCCTCATAGCGCCTGTACCTACGGGCAGACCCTGCTCTGTGTG
TCTTTTCCCTTCGCTCAGTTCTCGAAGATGGGCAAGCAGCTGACCTACACCTACCCCTGG
GTCTATAACTACCAGCTGGAAGGGATCTTTGCCCAGGAATTCCCCGACTTGGAGAATGTG
GTCAAGGGCCACCACGTTAGCCAAGAACCCTGGAACAGCAGCATCACACTCACATCCCAG
GCCGGGGCTGTTTTCCAGAGCTTTGCCAAGTTCAGCAAATTTGGAGATGACCTGTACTCC
GGCTGGTTGGCAGCAGCCCTTGGTACCAACCTGCAGGTCCAGTTCTGGCACAAAACTGTA
GGCATCCTGCCCTCTAACTGCTCGGATATCTGGCAGGTTCTGAATGTGAACCAGATAGCT
TTCCCTGGACCAGCCGGCCCAAGCTTCAACAGCACAGAGGACCACTCCAAATGGTGCGTG
TCCCCAAAAGGGCCCTGGACCTGCGTGGGTGACATGAATCGGAACCAGGGAGAGGAGCAA
CGGGGTGGGGGCACACTGTGTGCCCAGCTGCCAGCCCTCTGGAAAGCCTTCCAGCCGCTG
GTGAAGAACTACCAGCCCTGTAATGGCATGGCCAGGAAGCCCAGCAGAGCTTATAAGATC
TAA

Clone variation with respect to NM_001375.2
>OriGene 5' read for NM_001375 unedited
TAGATTTGTATACGACTCACTATAGGGNNCGGCCGCGCAATTCGGCACGAGGATGTAACC
CAGCGCCCCGCAGTCCCGACACAGATTCCTGGATCTCAGCCCCATAGCAGCTATGATCCC
GCTGCTGCTGGCAGCGCTGCTGTGCGTCCCCGCCGGGGCCCTGACCTGCTACGGGGACTC
CGGGCAGCCTGTAGACTGGTTCGTGGTCTACAAGCTGCCAGCTCTTAGAGGGTCCGGGGA
GGCGGCGCAGAGAGGGCTGCAGTACAAGTATCTGGACGAGAGCTCCGGAGGCTGGCGGGA
CGGCAGGGCACTCATCAACAGCCCGGAGGGGGCCGTGGGCCGAAGCCTGCAGCCGCTGTA
CCGGAGCAACACCAGCCAGCTCGCCTTCCTGCTCTACAATGACCAACCGCCTCAACCCAG
CAAGGCTCAGGACTCTTCCATGCGTGGGCACACGAAGGGTGTCCTGCTCCTTGACCACGA
TGGGGGCTTCTGGCTGGTCCACAGTGTACCTAACTTCCCTCCACCGGCCTCCTCTGCTGC
ATACAGCTGGCCTCATAGCGCCTGTACCTACGGGCAGACCCTGCTCTGTGTGTCTTTTCC
CTTCGCTCAGTTCTCGAAGATGGGCAAGCAGCTGACCTACACCTACCCCTGGGTCTATAA
CTACCAGCTGGAAGGGATCTTTGCCCAGGAATTCCCCGACTTGGAGAATGTGGTCAAGGG
CCACCACGTTAGCCAAGAACCCTGGAACAGCAGCATCACACTCACATCCCAGCCCCGGGC
TGTTTTCCAGAGCTCTGCCAAGTTCAGCAAATTTGGAGATGACCTGTACTCCGGCTGGTT
GGCAGCAGCCCTTGGTCCAACCTGCAGGTCCAGTTCCTGCACAAAACTGTTAGCATCCTG
CCCTCTAACTGCTCGNATATTTGGCAGGTTCTGAATGTGGACCCGCATACCTTCCCTGTA
CCAN
>OriGene 3' read for NM_001375 unedited
CTAGAGTCGAGTTTTTTTTTTTTTTTTTTAGATTTTTTATTTTTTATTTTTTTGACACAG
AGTCTCATTCTGTCACCCAGGCTGAAGTGCCGTGGTGTTAGCTCAGCTCATGACAACCTC
TGCCTCCCGAGTTCAAGCGATTCTCCTGCCTTACCCTCCCGAGGAGCTAGGACCACAGAC
GCGTGCCACCATGCCTGGCTAATCCTTAAACATTCTTAATGGATATGGGGTTTCACCATG
TTGGCCAGGCTGGCCTCGAACTCCTGACCTCAAGTGATCTGCCCATCTAAGACTCCCAAA
GTGCTGGGGCTACAGATGTGAGCCACTGCACCCACCCGTACACCGACCACCCTTTTTTTT
TGAAACAGAGCCTTGCTCTGTGACCTCACGCTGGCGCCCCACATAGCCCACTGTCACCAT
GATTCCCACTGGCCCAACCCATCCTCTGGCTTCACTGCGCCGGTACCCACAGGCCTTTAC
CACCCGCGCCTGCTTCCCTCTCTTCCAACTCCATCTCCACATGTGGCCTCCCTTTGCAAC
CCCCGCCTGTCCCCCCATCCCCTGCCTTCTAAGTGCACCCTTACCTCCTTCGACTTCGCC
AAGTCGCATGGAATACCATACCCGACCGCCTGCCCCTTGCCCCCAATGGCCTCCAACATT
ACTAACCCCTGCTGGGCCTACCCGGCCCATTCCATCACAGGGCTTGCCAACTCCCCCAAC
CCCCGTCCGTAACGATTATATTAAGGGCTCGCCCTCCGGGCCCCACAGTTGTGCCACACC
TCCCCACCACTATACATTCTTGCCACACCCCCCTGCGACACCCCCCCGTCTCGCGGCGAC
TTTTTCGGGACCCTCAACCCCTCTCTGCGTCGATTATACTCGCGCCTATATTAACCTTGG
CTTCATTTGACCACACCGCGCAANTGTGCTTGCCATATTTATCACGCCTTCCGCCTTTCC
CCTTCACCCCATTACGGCGACGCTCAATATAATACAGCGCTCATACACCCCGCATTCTCA
TCGCTNGTCCCCATGCGCTGCCGCACATTACCGCCCGTCCCGTGCCTCCCACTCCTCTAG
TCACGCCTCGCTCTANTCGCTNACCACACACCCACTCACGTTGTCGTG
Restriction Sites NotI-NotI     
ACCN NM_001375
Insert Size 1790 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001375.2, NP_001366.1
RefSeq Size 2011 bp
RefSeq ORF 1083 bp
Locus ID 1777
UniProt ID O00115
Domains DNase_II
Protein Families Druggable Genome
Protein Pathways Lysosome
Gene Summary This gene encodes a member of the DNase family. The protein, located in the lysosome, hydrolyzes DNA under acidic conditions and mediates the breakdown of DNA during erythropoiesis and apoptosis. Two codominant alleles have been characterized, DNASE2*L (low activity) and DNASE2*H (high activity), that differ at one nucleotide in the promoter region. The DNASE2*H allele is represented in this record. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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