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EIF4B (NM_001417) Human Untagged Clone
CAT＃: SC119269
EIF4B (untagged)-Human eukaryotic translation initiation factor 4B (EIF4B)
CNY 4,560.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | EIF-4B; PRO1843 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC119269 sequence for NM_001417 edited (data generated by NextGen Sequencing)
ATGGCGGCCTCAGCAAAAAAGAAGAATAAGAAGGGGAAGACTATCTCCCTAACAGACTTT CTGGCTGAGGATGGGGGTACTGGTGGAGGAAGCACCTATGTTTCCAAACCAGTCAGCTGG GCTGATGAAACGGATGACCTGGAAGGAGATGTTTCGACCACTTGGCACAGTAACGATGAC GATGTGTATAGGGCGCCTCCAATTGACCGTTCCATCCTTCCCACTGCTCCACGGGCTGCT CGGGAACCCAATATCGACCGGAGCCGTCTTCCCAAATCGCCACCCTACACTGCTTTTCTA GGAAACCTACCCTATGATGTTACAGAAGAGTCAATTAAGGAATTCTTTCGAGGATTAAAT ATCAGTGCAGTGCGTTTACCACGTGAACCCAGCAATCCAGAGAGGTTGAAAGGTTTTGGT TATGCTGAATTTGAGGACCTGGATTCCCTGCTCAGTGCCCTGAGTCTCAATGAAGAGTCT CTAGGTAACAGGAGAATTCGAGTGGACGTTGCTGATCAAGCACAGGATAAAGACAGGGAT GATCGTTCTTTTGGCCGTGATAGAAATCGGGATTCTGACAAAACAGATACAGACTGGAGG GCTCGTCCTGCTACAGACAGCTTTGATGACTACCCACCTAGAAGAGGTGATGATAGCTTT GGAGACAAGTATCGAGATCGTTATGATTCAGACCGGTATCGGGATGGGTATCGGGATGGG TATCGGGATGGCCCACGCCGGGATATGGATCGATATGGTGGCCGGGATCGCTATGATGAC CGAGGCAGCAGAGACTATGATAGAGGCTATGATTCCCGGATAGGCAGTGGCAGAAGAGCA TTTGGCAGTGGGTATCGCAGGGATGATGACTACAGAGGAGGCGGGGACCGCTATGAAGAC CGATATGACAGACGGGATGATCGGTCGTGGAGCTCCAGAGATGATTACTCTCGGGATGAT TATAGGCGTGATGATAGAGGTCCCCCCCAAAGACCCAAACTGAATCTAAAGCCTCGGAGT ACTCCTAAGGAAGATGATTCCTCTGCTAGTACCTCCCAGTCCACTCGAGCTGCTTCTATC TTTGGAGGGGCAAAGCCTGTTGACACAGCTGCTAGAGAAAGAGAAGTAGAAGAACGGCTA CAGAAGGAACAAGAGAAGTTGCAGCGTCAGCTGGATGAGCCAAAACTAGAACGACGGCCT CGGGAGAGACACCCAAGCTGGCGAAGTGAAGAAACTCAGGAACGGGAACGGTCGAGGACA GGAAGTGAGTCATCACAAACTGGGACCTCCACCACATCTAGCAGAAATGCACGAAGGAGA GAGAGTGAGAAGTCTCTAGAAAATGAAACACTCAATAAGGAGGAAGATTGCCACTCTCCA ACTTCTAAACCTCCCAAACCTGATCAGCCCCTAAAGGTAATGCCAGCCCCTCCACCAAAG GAGAATGCTTGGGTGAAGCGAAGTTCTAACCCTCCTGCTCGATCTCAGAGCTCAGACACA GAGCAGCAGTCCCCTACAAGTGGTGGGGGAAAAGTAGCTCCAGCTCAACCATCTGAGGAA GGACCAGGAAGGAAAGATGAAAATAAAGTAGATGGGATGAATGCCCCAAAAGGCCAAACT GGGAACTCTAGCCGTGGTCCAGGAGACGGAGGGAACAGAGACCACTGGAAGGAGTCAGAT AGGAAAGATGGCAAAAAGGATCAAGACTCCAGATCTGCACCTGAGCCAAAGAAACCTGAG GAAAATCCAGCTTCYAAGTTCAGTTCTGCAAGCAAGTATGCTGCTCTCTCTGTTGATGGT GAAGATGAAAATGAGGGAGAAGATTATGCCGAATAG Clone variation with respect to NM_001417.4 1755 c=>y >OriGene 5' read for NM_001417 unedited
NGGCGTGTCATATTTGTATACGACTCATATAGGCGGCNCGCGAATTCGCACGAGGCTCCC AACATGGCGGCCTCAGCAAAAAAGAAGAATAAGAAGGGGAAGACTATCTCCCTAACAGAC TTTCTGGCTGAGGATGGGGGTACTGGTGGAGGAAGCACCTATGTTTCCAAACCAGTCAGC TGGGCTGATGAAACGGATGACCTGGAAGGAGATGTTTCGACCACTTGGCACAGTAACGAT GACGATGTGTATAGGGCGCCTCCAATTGACCGTTCCATCCTTCCCACTGCTCCACGGGCT GCTCGGGAACCCAATATCGACCGGAGCCGTCTTCCCAAATCGCCACCCTACACTGCTTTT CTAGGAAACCTACCCTATGATGTTACAGAAGAGTCAATTAAGGAATTCTTTCGAGGATTA AATATCAGTGCAGTGCGTTTACCACGTGAACCCAGCAATCCAGAGAGGTTGAAAGGTTTT GGTTATGCTGAATTTGAGGACCTGGATTCCCTGCTCAGTGCCCTGAGTCTCAATGAAGAG TCTCTAGGTAACAGGAGAATTCGAGTGGACGTTGCTGATCAAGCACAGGATAAAGACAGG GATGATCGTTCTTTTGGCCGTGATAGAAATCGGGATTCTGACAAAACAGATACAGACTGG AGGGCTCGTCCTGCTACAGACAGCTNTGATGACTACCCACCTAGAAGAGGTGATGATAGC TNTGGAGACAAGTATCGAGATCGTTATGATTCAGACCGGTATCGGGATGGGTATCGGNAT GGGGTATCGGATGGCCCACGCCGGGATATGGATCGATATGGTGGCCCGGNATCGCTATGA TGACCCGAGCAGCAGAGACTATGATAGAGGCTATGATTCCCGNATAGCAGTGGCAGAAGA GCATTTGNCA >OriGene 3' read for NM_001417 unedited
CTATGTACGCGGCCGCAATCTATAGTCGAGTNTTTTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTTTTAAAGAAAAGGGGGATTTTTATTTTGTCTTGTCGGAATA AAGGTTTTGATTTGCTCTCAAATGTCCCAGGGGGGAAAAAACCTAGGAAAAAAACCCCAG GATGTAAAGGCCTTTTCGGCATAATCTTCCCCCTCTTTTTCTTCTTCCCCATCAACAAAA AAACCACCTTCCTTGCTTGCAAAACTGAACTTGAAAGCTGGATTTTCCTCAGGTTTTTTT GGCTCAGGGGCAAATCGGGAGTCTTGACCCTTTTTGCCATCTTTCCTATCTGACTCCTTC CAGGGGTCTTTGTCCCCTCCGTCTCCTGAACCACGGTTAAATTTCCCATTTGGGCCTTTG GGGGCTTTCACCCCATCAACTTTATTTTCATCTTTCCTTCCGGGCCCTTCCTAAAATGGG GGAGCGGGACCTACTTTTCCCCCACCACTTGTAGGGAACTGCTGCTCTGGGTCTGACCTC TGAAATCAACCAGGAGGGTTAAAACCTTCCTTTCCCCAACCATTCTCCTTTGGGGGAGGG GCGGGCTTTACCTTTAGGGGCTGACCAGGTTGGGAAGGTCTAAAATTCGGAAATGGGCAT CCTCCCCCTTTTGGAGGGTTAATTTCCTAGAAACTTTCCCTCCTTTCCTCTTCGGCTTTC TGCAAATGGGGGGGAGGCCCCCGTCTGGGATACCCCCTACTTGTCCCAACCGCCCCGTAC TCGATTTCTTCCCTACACCATTTGCGTGTACACTCCACGTCCTCTCTTACTACCTCTCTC CCCCTCTCCACGCTCTCTTCTCACTTCCTCACCCTCTCACCACCCTCTTTGCCCCATCCT TTCTTTTCTTATCCCCACCCCCCCTCCCTATCTCTCTTTCACCCACACCACCCCACTTCC TCTCATCCTCTCATACCTCTT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_001417 |
| Insert Size | 2090 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001417.2, NP_001408.2 |
| RefSeq Size | 3919 bp |
| RefSeq ORF | 1836 bp |
| Locus ID | 1975 |
| UniProt ID | P23588 |
| Domains | RRM |
| Protein Pathways | mTOR signaling pathway |
| Gene Summary | Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209955 | EIF4B (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 4B (EIF4B) |
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| RC209955L1 | Lenti ORF clone of Human eukaryotic translation initiation factor 4B (EIF4B), Myc-DDK-tagged |
CNY 6,952.00 |
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| RC209955L2 | Lenti ORF clone of Human eukaryotic translation initiation factor 4B (EIF4B), mGFP tagged |
CNY 5,890.00 |
|
| RC209955L3 | Lenti ORF clone of Human eukaryotic translation initiation factor 4B (EIF4B), Myc-DDK-tagged |
CNY 6,952.00 |
|
| RC209955L4 | Lenti ORF clone of Human eukaryotic translation initiation factor 4B (EIF4B), mGFP tagged |
CNY 5,890.00 |
|
| RG209955 | EIF4B (tGFP-tagged) - Human eukaryotic translation initiation factor 4B (EIF4B) |
CNY 6,152.00 |
