United States
Germany
Japan
United Kingdom
China
GCKR (NM_001486) Human Untagged Clone
CAT＃: SC119244
GCKR (untagged)-Human glucokinase (hexokinase 4) regulator (GCKR)
CNY 6,992.00
| Cited in 2 publications. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | FGQTL5; GKRP |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC119244 sequence for NM_001486 edited (data generated by NextGen Sequencing)
ATGCCAGGCACAAAACGGTTTCAACATGTCATTGAGACCCCGGAGCCTGGCAAGTGGGAG TTGTCTGGGTACGAGGCAGCTGTGCCAATCACGGAGAAGTCAAACCCACTGACCCAGGAT CTAGACAAAGCAGATGCTGAGAACATTGTTCGACTGCTAGGGCAATGTGATGCTGAGATC TTCCAGGAGGAGGGGCAAGCCCTGTCCACATACCAGAGACTCTACAGCGGATCCATTCTG ACCACCATGGTACAGGTGGCTGGGAAAGTTCAGGAAGTGCTGAAGGAGCCAGATGGGGGG CTGGTTGTGCTGAGTGGAGGGGGCACCTCTGGCCGGATGGCATTCCTCATGTCGGTGTCC TTTAATCAGCTGATGAAAGGTCTGGGACAGAAACCTCTTTACACCTACCTCATTGCAGGT GGTGACAGGTCTGTGGTGGCCTCTAGGGAGGGGACAGAAGATAGTGCCTTGCACGGGATT GAGGAACTGAAGAAGGTGGCTGCCGGGAAGAAGAGAGTGATTGTCATTGGCATTTCTGTG GGACTCTCTGCTCCCTTTGTGGCAGGCCAGATGGACTGCTGCATGAACAACACAGCTGTC TTCTTGCCAGTCCTGGTTGGCTTCAATCCAGTGAGCATGGCCAGAAATGACCCCATTGAA GACTGGAGTTCAACATTCCGACAAGTAGCAGAGCGGATGCAGAAAATGCAGGAGAAACAG AAAGCTTTTGTGCTCAATCCTGCCATCGGGCCCGAGGGTCTCAGCGGCTCCTCCCGGATG AAAGGTGGAAGTGCCACCAAGATTCTGCTGGAAACCCTGTTATTAGCAGCCCATAAGACT GTGGACCAGGGCATTGCAGCATCTCAAAGATGCCTCCTGGAAATCTTGCGGACATTTGAG CGAGCTCATCAGGTGACCTACAGCCAAAGCCCCAAGATTGCCACCCTGATGAAGAGTGTC AGCACCAGTCTGGAGAAGAAAGGCCACGTGTACCTGGTTGGCTGGCAGACCCTGGGCATC ATTGCCATCATGGATGGAGTAGAGTGCATCCACACCTTTGGTGCTGATTTCCGAGATGTC CGTGGCTTTCTCATTGGTGATCACAGTGACATGTTTAACCAGAAGGCTGAGCTCACCAAC CAGGGTCCCCAGTTCACCTTCTCCCAGGAGGACTTCCTGACTTCCATCCTTCCCTCTCTC ACGGAAATCGATACTGTGGTCTTCATTTTCACCCTGGATGACAACCTCACGGAGGTGCAG ACTATAGTGGAGCAGGTGAAAGAGAAGACCAACCACATCCAGGCCCTGGCACACAGCACC GTGGGTCAGACCTTGCCGATCCCTCTGAAGAAGCTCTTTCCCTCCATCATCAGCATCACA TGGCCACTGCTTTTCTTTGAATATGAAGGGAACTTCATCCAGAAGTTCCAGCGTGAGCTA AGCACCAAATGGGTGCTGAATACAGTGAGTACAGGTGCTCATGTGCTTCTTGGTAAGATC CTACAAAACCACATGTTGGACCTTCGGATTAGCAACTCCAAGCTCTTCTGGCGGGCGCTG GCCATGCTGCAGCGGTTCTCTGGACAGTCCAAGGCTCGATGCATCGAGAGCCTCCTCCGA GCGATCCACTTTCCCCAGCCACTGTCAGATGATATTCGGGCTGCTCCCATCTCCTGCCAT GTCCAGGTTGCACATGAGAAGGAACAGGTGATACCCATCGCCTTGCTGAGCCTCCTATTC CGGTGCTCGATCACTGAGGCTCAGGCACACCTGGCTGCAGCTCCTTCTGTCTGTGAGGCT GTCAGGAGTGCTCTTGCTGGGCCAGGTCAGAAGCGCACTGCGGACCCCCTCGAGATCCTA GAGCCTGACGTTCAGTGA Clone variation with respect to NM_001486.3 230 a=>g;1337 t=>c >OriGene 5' read for NM_001486 unedited
GTTAGCATTTGTATACTATCACTATAGGCGGCACGCGATTCGGCACGATCTGAAGAGCAG GAGGAACAGTGTATCCACAGCGTGGGACCATGCCAGGCACAAAACGGTTTCAACATGTCA TTGAGACCCCGGAGCCTGGCAAGTGGGAGTTGTCTGGGTACGATGCAGCTGTGCCAATCA CGGAGAAGTCAAACCCACTGACCCATGATCTATACAAAGCAGATGCTGATAACATTGTTC GACTGCTAAGGCAATGTGATGCTGAGATCTTCCAGGAGGAGGGGCAAGCCCTGTCCACAT ACCATAGACTCTACAGCGGATCCATTCTGACCACCATGGTACAGGTGGCTGGGAAAGTTC ATGAAGTGCTGAAGGATCCAGATGGGGGGCTGGTTGTGCTGATTGGAGGGGGCACCTCTG GCCGGATGGCATTCCTCATGTCGGTGTCCTTTAATCAGCTGATGAAAGGTCTGGGACAGA AACCTCTTTACACCTACCTCATTGCAGGTGGTGACAGGTCTGTGGTGGCCTCTATGGAGG GGACAGAAGATAGTGCCTTGCACGGGATTGAAGAACTGAAGAATGTGGCTGCCGGGAAGA ATATAGTGATTGTCATTGGCATTTCTGTGGGACTCTCTGCTCCCTTTGTGGCAGGCCAGA TGGACTGCTGCATGAACAACACAGCTGTCTTCTTGCCAGTCCTGGTTGGCTTCAATCCAG TGAGCATGGCCAGAAATGACCCCATTGTAGACTGGAGTTCAACATTCCGACAAGTAGCAT AGCGGATGCAGAAAATGCAAGATAAACAGAAAGCTTTTGTGCTCAATCCTGCCATCAGGC CCCGATGGTCTCATCGGCTCCTNCCCGATGATATGTGGGAGTGCCCACAAGATTCTGCTG GAAAACCTGTTATTATCAGCCATTAGACTGTGCAC >OriGene 3' read for NM_001486 unedited
GTTGACCATTGGCTGATGGCAACTTGCCAGGNCCAGTATAGGCCTGGGGNAGGGTCACAG GGCATGCCACCCGGGTATCTGTTCAGGCAAACAGCTATGACCGCGGCCGCAATCTAGAGT CGAGTTTTTTTTTTTTTTTTTTTTTTTTAACTTTCCAAAATAAGACATTTCATTTTATTT CTGAAATCAGAATAAGTCGGTGAGAGTAGAAACCACTAGGTCGAGAGCAAGAACTCTCCC CCAAAGTGGAGAGAATATTTCTCCCTACCCTGGGCTGCGGATGCCCTGGAAACGGGGCTT CTTCCTCCCACATGTTCTGCTGGCACAAGTCCCCTTGGGCGGGCTGGGCTGAAGTGGGCA GGGTTGGGCCCCTTTCACCCACCCAGAAACATGGGTTCACTGAACGTCAGGCTCTAGGAT CTCGAGGGGGTCCGCAGTGCGCTTCTGACCTGGCCCAGCAAGAGCACTCCTGACAGCCTC ACAGACAGAAGGAGCTGCAGCCAGGTGTGCCTGAGCCTCAGTGATCGAGCACCGGAATAG GAGGCTCAGCAAGGCGATGGGTATCACCTGTTCCTTCTCATGTGCAACCTGGACATGGCA GGAGATGGGAGCAGCCCGAATATCATCTGACAGTGGCTGGGGAAAGTGGATCGCTCGGAG GAGGCTCTCGATGCATCGAGCCTTGGACTGTCCAGAGAACCGCTGCAGCATGGCCAGCGC CCGCCAGAAGAGCTTGGAGTTGCTAATCCGAAGTCCAACATGTGGTTGTGTAGGATCTTA CCAAGAAGCACATGAGCACCTGTACTCACTGTATTCAGCACCCTTTTTGTGCTTAGCTCA CGCTGGAACTTCTGGATGAAGTTCCCTTCATATTCAAGAAAAACAGTGGCCATGTGATGC TGAT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_001486 |
| Insert Size | 2250 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001486.2, NP_001477.1 |
| RefSeq Size | 2197 bp |
| RefSeq ORF | 1878 bp |
| Locus ID | 2646 |
| UniProt ID | Q14397 |
| Domains | SIS |
| Gene Summary | This gene encodes a protein belonging to the GCKR subfamily of the SIS (Sugar ISomerase) family of proteins. The gene product is a regulatory protein that inhibits glucokinase in liver and pancreatic islet cells by binding non-covalently to form an inactive complex with the enzyme. This gene is considered a susceptibility gene candidate for a form of maturity-onset diabetes of the young (MODY). [provided by RefSeq, Jul 2008] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Functional Variant in the GCKR Gene Affects Lactate Levels Differentially in the Fasting State and During Hyperglycemia
,López Rodríguez, M;Fernandes Silva, L;Vangipurapu, J;Modi, S;Kuusisto, J;Kaikkonen, MU;Laakso, M;,
Sci Rep
,PubMed ID 30375486
[GCKR]
|
|
Role of glucokinase in the subcellular localization of glucokinase regulatory protein
,Jin, L;Guo, T;Li, Z;Lei, Z;Li, H;Mao, Y;Wang, X;Zhou, N;Zhang, Y;Hu, R;Zhang, X;Niu, G;Irwin, DM;Tan, H;,
Int J Mol Sci
,PubMed ID 25849650
[GCKR]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214230 | GCKR (Myc-DDK-tagged)-Human glucokinase (hexokinase 4) regulator (GCKR) |
CNY 6,976.00 |
|
| RC214230L1 | Lenti ORF clone of Human glucokinase (hexokinase 4) regulator (GCKR), Myc-DDK-tagged |
CNY 9,376.00 |
|
| RC214230L2 | Lenti ORF clone of Human glucokinase (hexokinase 4) regulator (GCKR), mGFP tagged |
CNY 5,890.00 |
|
| RC214230L3 | Lenti ORF clone of Human glucokinase (hexokinase 4) regulator (GCKR), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC214230L4 | Lenti ORF clone of Human glucokinase (hexokinase 4) regulator (GCKR), mGFP tagged |
CNY 5,890.00 |
|
| RG214230 | GCKR (tGFP-tagged) - Human glucokinase (hexokinase 4) regulator (GCKR) |
CNY 8,576.00 |
