GPR32 (NM_001506) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	RVDR1
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >SC119212 representing NM_001506. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGAATGGGGTCTCGGAGGGGACCAGAGGCTGCAGTGACAGGCAACCTGGGGTCCTGACACGTGATCGC TCTTGTTCCAGGAAGATGAACTCTTCCGGATGCCTGTCTGAGGAGGTGGGGTCCCTCCGCCCACTGACT GTGGTTATCCTGTCTGCGTCCATTGTCGTCGGAGTGCTGGGCAATGGGCTGGTGCTGTGGATGACTGTC TTCCGTATGGCACGCACGGTCTCCACCGTCTGCTTCTTCCACCTGGCCCTTGCCGATTTCATGCTCTCA CTGTCTCTGCCCATTGCCATGTACTATATTGTCTCCAGGCAGTGGCTCCTCGGAGAGTGGGCCTGCAAA CTCTACATCACCTTTGTGTTCCTCAGCTACTTTGCCAGTAACTGCCTCCTTGTCTTCATCTCTGTGGAC CGTTGCATCTCTGTCCTCTACCCCGTCTGGGCCCTGAACCACCGCACTGTGCAGCGGGCAAGCTGGCTG GCCTTTGGGGTGTGGCTCCTGGCCGCCGCCTTGTGCTCTGCGCACCTGAAATTCCGGACAACCAGAAAA TGGAATGGCTGTACGCACTGCTACTTGGCGTTCAACTCTGACAATGAGACTGCCCAGATTTGGATTGAA GGGGTCGTGGAGGGACACATTATAGGGACCATTGGCCACTTCCTGCTGGGCTTCCTGGGGCCCTTAGCA ATCATAGGCACCTGCGCCCACCTCATCCGGGCCAAGCTCTTGCGGGAGGGCTGGGTCCATGCCAACCGG CCCAAGAGGCTGCTGCTGGTGCTGGTGAGCGCTTTCTTTATCTTCTGGTCCCCGTTTAACGTGGTGCTG TTGGTCCATCTGTGGCGACGGGTGATGCTCAAGGAAATCTACCACCCCCGGATGCTGCTCATCCTCCAG GCTAGCTTTGCCTTGGGCTGTGTCAACAGCAGCCTCAACCCCTTCCTCTACGTCTTCGTTGGCAGAGAT TTCCAAGAAAAGTTTTTCCAGTCTTTGACTTCTGCCCTGGCGAGGGCGTTTGGAGAGGAGGAGTTTCTG TCATCCTGTCCCCGTGGCAACGCCCCCCGGGAATGA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_001506 unedited NAAACACTCTAGATTTGTTAAACGNACTTTACTATCAGGGCGTGGCGCCAGTGTGNATGG GATATCTGTCANAATTCGAGCTTATGGCAATGGNAGAAATCATGAATGGGGTCTCGGAGG GGACCAGAGGCTGCAGTGACAGGCAACCTGGGGTCCTGACACGTGATCGCTCTTGTTCCA GGAAGATGAACTCTTCCGGATGCCTGTCTGAGGAGGTGGGGTCCCTCCGCCCACTGACTG TGGTTATCCTGTCTGCGTCCATTGTCGTCGGAGTGCTGGGCAATGGGCTGGTGCTGTGGA TGACTGTCTTCCGTATGGCACGCACGGTCTCCACCGTCTGCTTCTTCCACCTGGCCCTTG CCGATTTCATGCTCTCACTGTCTCTGCCCATTGCCATGTACTATATTGTCTCCAGGCAGT GGCTCCTCGGAGAGTGGGCCTGCAAACTCTACATCACCTTTGTGTTCCTCAGCTACTTTG CCAGTAACTGCCTCCTTGTCTTCATCTCTGTGGACCGTTGCATCTCTGTCCTCTACCCCG TCTGGGCCCTGAACCACCGCACTGTGCAGCGGGCAAGCTGGCTGGCCTTTGGGGTGTGGC TCCTGGCCGCCGCCTTGTGCTCTGCGCACCTGAAATTCCGGACAACCAGAAAATGGAATG GCTGTACGCACTGCTACTTGGCGTTCAACTCTGACAATGAGACTGCCCAGATTTGGATTG AAGGGGTCGTGGAGGGACACATTATAGGGACCATTGGCCACTTCCTGCTGGGCTTCCTGG GGCCCTTAGCATCATAGGCACCTGCGCCCACCTCATCCGGGCCAAGCTCTTGCAGGAGGG CTGGGTNCCATGCCAACCGCCCAAGAGCTGCTGCTGGTGCTGGTGAGCGCTTTTCTTATC TTCTGGNTCCCGTTTAACGTGGTGCTGTTGGTCCATCTGGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001506
Insert Size	1071 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001506.1
RefSeq Size	1071 bp
RefSeq ORF	1071 bp
Locus ID	2854
UniProt ID	O75388
Protein Families	Druggable Genome, GPCR, Transmembrane
MW	40.1 kDa
Gene Summary	This gene is intronless and encodes a member of the G-protein coupled receptor 1 family. The encoded protein binds to resolvin D1 and lipoxin A4 and has been linked to pulmonary inflammation. A related pseudogene has been identified on chromosome 19. [provided by RefSeq, Nov 2012]

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