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NDST1 (NM_001543) Human Untagged Clone
CAT＃: SC119198
NDST1 (untagged)-Human N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 1 (NDST1)
CNY 17,010.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | HSST; MRT46; NST1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_001543, the custom clone sequence may differ by one or more nucleotides
ATGCCTGCCCTGGCATGCCTCCGGAGGCTGTGTCGGCACGTGTCCCCGCAGGCTGTCCTTTTCCTGCTGT TCATCTTCTGCCTGTTCAGCGTTTTCATCTCGGCCTACTACCTATATGGCTGGAAGCGAGGCCTGGAGCC CTCGGCGGATGCCCCCGAGCCTGACTGCGGGGACCCGCCGCCTGTGGCCCCCAGTCGCCTGCTGCCACTC AAGCCTGTGCAGGCAGCCACCCCTTCCCGCACAGACCCGTTGGTGCTGGTCTTTGTGGAGAGCCTCTACT CGCAACTGGGCCAGGAGGTGGTGGCCATCCTGGAGTCCAGCCGCTTCAAATACCGCACAGAGATTGCGCC GGGCAAGGGTGACATGCCCACGCTCACTGACAAGGGCCGTGGCCGCTTCGCCCTCATCATCTATGAGAAC ATCCTCAAGTATGTCAACCTGGACGCCTGGAACCGGGAGCTGCTGGACAAGTACTGTGTGGCCTACGGCG TGGGCATCATTGGCTTCTTCAAGGCCAATGAGAACAGCCTGCTGAGTGCGCAGCTCAAGGGCTTCCCCCT GTTCCTGCACTCAAACCTGGGCCTGAAGGACTGCAGCATCAACCCCAAGTCCCCGCTGCTCTACGTGACG CGACCTAGCGAGGTGGAGAAAGGTGTGCTCCCCGGCGAGGACTGGACGGTTTTCCAGTCAAATCACTCCA CCTATGAGCCAGTGCTGCTGGCCAAGACGCGCTCGTCTGAGTCCATCCCACACCTGGGCGCAGACGCCGG CCTGCATGCTGCACTGCACGCCACTGTGGTCCAGGACCTGGGCCTGCACGACGGCATCCAGCGCGTGCTG TTTGGCAACAACCTGAACTTCTGGCTGCACAAGCTTGTCTTCGTGGATGCCGTGGCCTTCCTCACGGGGA AGCGCCTCTCCCTGCCATTGGACCGCTACATCCTGGTGGACATTGATGACATCTTCGTGGGCAAGGAGGG CACACGCATGAAGGTGGAGGACGTGAAGGCCCTGTTTGACACACAGAACGAACTACGCGCACACATCCCA AACTTCACCTTCAACCTGGGCTACTCAGGGAAATTCTTCCACACAGGTACCAATGCTGAGGACGCTGGGG ATGATCTGCTGCTGTCGTATGTGAAGGAGTTCTGGTGGTTCCCCCACATGTGGAGCCACATGCAGCCCCA CCTTTTCCACAACCAGTCCGTGTTGGCCGAGCAGATGGCCTTGAACAAGAAGTTCGCTGTCGAGCATGGC ATTCCCACAGACATGGGGTATGCAGTGGCGCCCCACCACTCGGGCGTGTACCCCGTGCACGTGCAGCTGT ACGAGGCTTGGAAGCAGGTGTGGAGCATCCGCGTGACCAGCACGGAGGAGTACCCCCACCTGAAGCCAGC CCGCTACCGCCGTGGCTTCATCCACAATGGCATCATGGTTCTCCCACGGCAGACCTGCGGCCTCTTCACA CACACCATCTTCTACAACGAGTACCCTGGCGGCTCCAGTGAGCTGGACAAGATCATCAACGGGGGCGAGC TCTTCCTCACCGTGCTCCTCAATCCTATCAGCATCTTCATGACGCACCTGTCCAACTATGGGAATGACCG CCTGGGCCTGTACACCTTCAAGCACCTGGTGCGCTTCCTGCACTCCTGGACGAACCTCCGGCTGCAGACA CTGCCCCCTGTGCAGTTGGCGCAGAAGTACTTCCAGATCTTCTCCGAGGAGAAGGACCCGCTCTGGCAGG ACCCCTGCGAGGACAAACGTCACAAAGACATCTGGTCCAAGGAGAAGACGTGTGACCGCTTCCCAAAGCT CCTCATCATCGGCCCCCAGAAAACAGGCACCACTGCCCTCTACCTGTTCCTGGGCATGCACCCTGACCTA AGCAGCAACTACCCCAGCTCTGAGACCTTTGAGGAGATCCAGTTTTTTAATGGCCACAACTATCACAAAG GCATCGACTGGTACATGGAGTTCTTCCCCATCCCTTCCAACACCACCTCCGACTTCTACTTTGAGAAAAG CGCCAACTACTTTGATTCAGAAGTGGCGCCCCGGCGGGCAGCAGCCCTCTTGCCCAAAGCCAAGGTCCTG ACCATCCTCATCAACCCCGCGGACCGGGCCTATTCCTGGTACCAGCACCAGCGAGCCCATGACGACCCAG TGGCCCTAAAGTACACCTTCCATGAGGTGATTACCGCCGGCTCTGACGCATCCTCGAAGCTGCGTGCCCT CCAGAACCGCTGCCTGGTCCCTGGCTGGTACGCCACCCACATCGAGCGCTGGCTCAGTGCCTATCACGCC AACCAGATTCTGGTCTTGGATGGCAAACTGCTTCGCACAGAACCTGCCAAAGTGATGGACATGGTGCAGA AGTTCCTTGGGGTGACCAACACCATTGACTACCACAAAACCTTGGCGTTTGATCCAAAGAAAGGATTTTG GTGCCAACTGCTTGAAGGAGGAAAAACCAAGTGTCTGGGCAAAAGCAAGGGCCGGAAATATCCCGAGATG GACTTGGATTCCCGAGCCTTCCTGAAGGACTATTACCGGGACCACAACATCGAGCTCTCCAAGCTGCTGT ATAAGATGGGCCAGACACTTCCCACTTGGCTACGAGAGGACCTCCAGAACACCAGGTAG >OriGene 5' read for NM_001543 unedited
TTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGCCTCCGGTGGCCA AGGTCTCGGAGGCCAGGATGCCTGCCCTGGCATGCCTCCGGAGGCTGTGTCGGCACGTGT CCCCGCAGGCTGTCCTTTTCCTGCTGTTCATCTTCTGCCTGTTCAGCGTTTTCATCTCGG CCTACTACCTATATGGCTGGAAGCGAGGCCTGGAGCCCTCGGCGGATGCCCCCGAGCCTG ACTGCGGGGACCCGCCGCCTGTGGCCCCCAGTCGCCTGCTGCCACTCAAGCCTGTGCAGG CAGCCACCCCTTCCCGCACAGACCCGTTGGTGCTGGTCTTTGTGGAGAGCCTCTACTCGC AACTGGGCCAGGAGGTGGTGGCCATCCTGGAGTCCAGCCGCTTCAAATACCGCACAGAGA TTGCGCCGGGCAAGGGTGACATGCCCACGCTCACTGACAAGGGCCGTGGCCGCTTCGCCC TCATCATCTATGAGAACATCCTCAAGTATGTCAACCTGGACGCCTGGAACCGGGAGCTGC TGGACAAGTACTGTGTGGCCTACGGCGTGGGCATCATTGGCTTCTTCAAGGCCAATGAGA ACAGCCTGCTGAGTGCGCAGCTCAAGGGCTTCCCCCTGTTCCTGCACTCAAACCTGNGCC TGAAGGACTGCAGCATCAACCCCAAGTCCCCGCTGCTCTACGTGACGCGACCTANCGAGG TGGAGAAAAGGTGTGCTCCCCGGCCGAGACTGGNACGGNTTTTNCAGTCAAATCACTCCA CCCTATGAGCAGNTGCTGCTGGCCAAGACGCGCCTCGTCTGAGTCCATCCACACCTGGGC GCANACGCCNGCCTGCATGCTGACTGACGCCACTGTGGTCANGACCCTGGCTGNACGACG CTTCCAAGGNGTGTGGTTGTTGGCACAACCTGACTCTGCTGACN >OriGene 3' read for NM_001543 unedited
NNGTACTTGNACCCGGCCGCAATCTANGATCGAGTTTTTTTTTTTTTTTTTTATTTTAGC TTCTTAGTATTTGGGAGGTTAAGGGGGTCTCTGAGAGCCCTCGGCCACAGGTGGCTAAGC AAAGCCGTGGCTGGCAGTCTCACCAGCTCGCCCATGTCTGCCTGGGCCTGAGGCTGGGAG GGTGTGGGGACCGTGTCTGTACATGGGAACATGTTACTGGCAGACCCCTGGCCTGCCTCA GTCTTTTACCACAGGAAACACCACACATCATATCCCAGGGGGAAGAAAGGGGTGCAGGTG GGGCCCGGAGAACTGGGCCCGATGGCAAGGCAGTGCCCCAAGGCCCTGCGGGAAGCCTTG AGGATGGAGTGGATACTGTAGGAACCAGAAATTCTTCCCCAGAACGGGAGGGTGAGATGT GAAGGTAGGAACCAGATGTGGAGGCTGGGAGTCCTCTTGAGGGGGCCACCCTCCCATCCA GCCTAGGACTGACTCCGGGGACCCAGCTGGCCCCTTGGAAGAGCCTGGCTCGAAACAGGG AAATGTCCAGTCTGTCTCCCCCGGCCAGGCTCTCCTCCCCTACAGCCTGGAGGACAGGGA CATATGGCAGCGAATACTGAGGGGCGCCCTGCGNAAGCAGGGAGTGATGGGTGACAANAG GGGACAGTCGGCNGAACACAGTGAGGGACAGAAAAGAGTCTCGTGGACTCCCTCCTACCA GAAAGCGGCCTCCCCAGGAGCACCTGGGAACGAATGAACCCCTGCTGGCGAGGGAGGTCC CTTAGGCGCAAACCAGCGTGGGTGCTCCGAGGGGCTGCAAATCGCCTGGGTGCTCCCCAC CCCACCAAACCTTCCAAAATATGGTCATAATGCGCCATTGCCCTGTCCAGTTTCCATTTT GAGGTTTGGTAACGGTGGGGGGATTGC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_001543 |
| Insert Size | 3670 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001543.3, NP_001534.1 |
| RefSeq Size | 7913 bp |
| RefSeq ORF | 2649 bp |
| Locus ID | 3340 |
| UniProt ID | P52848 |
| Domains | Sulfotransfer |
| Protein Families | Transmembrane |
| Protein Pathways | Heparan sulfate biosynthesis, Metabolic pathways |
| Gene Summary | This gene encodes a member of the heparan sulfate/heparin GlcNAc N-deacetylase/ N-sulfotransferase family. The encoded enzyme is a type II transmembrane protein that resides in the Golgi apparatus. The encoded protein catalyzes the transfer of sulfate from 3'-phosphoadenosine 5'-phosphosulfate to nitrogen of glucosamine in heparan sulfate. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014] Transcript Variant: This variant (1) encodes the longer isoform (1). |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221638 | NDST1 (Myc-DDK-tagged)-Human N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 1 (NDST1) |
CNY 6,464.00 |
|
| RC221638L3 | Lenti ORF clone of Human N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 1 (NDST1), Myc-DDK-tagged |
CNY 9,030.00 |
|
| RC221638L4 | Lenti ORF clone of Human N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 1 (NDST1), mGFP tagged |
CNY 9,030.00 |
|
| RG221638 | NDST1 (tGFP-tagged) - Human N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 1 (NDST1) |
CNY 7,890.00 |
