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HIF-1 alpha (HIF1A) (NM_001530) Human Untagged Clone
CAT＃: SC119189
HIF1A (untagged)-Human hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) (HIF1A), transcript variant 1
CNY 9,088.00
货期*
现货
规格
| Cited in 14 publications. |
Product images
经常一起买 (3)
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | bHLHe78; HIF-1-alpha; HIF-1A; HIF-1alpha; HIF1; HIF1-ALPHA; MOP1; PASD8 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_001530 edited
GAATTCGGCACGAGGCCTCTTCGTCGCTTCGGCCAGTGTGTCGGGCTGGGCCCTGACAAG CCACCTGAGGAGAGGCTCGGAGCCGGGCCCGGACCCCGGCGATTGCCGCCCGCTTCTCTC TAGTCTCACGAGGGGTTTCCCGCCTCGCACCCCCACCTCTGGACTTGCCTTTCCTTCTCT TCTCCGCGTGTGGAGGGAGCCAGCGCTTAGGCCGGAGCGAGCCTGGGGGCCGCCCGCCGT GAAGACATCGCGGGGACCGATTCACCATGGAGGGCGCCGGCGGCGCGAACGACAAGAAAA AGATAAGTTCTGAACGTCGAAAAGAAAAGTCTCGAGATGCAGCCAGATCTCGGCGAAGTA AAGAATCTGAAGTTTTTTATGAGCTTGCTCATCAGTTGCCACTTCCACATAATGTGAGTT CGCATCTTGATAAGGCCTCTGTGATGAGGCTTACCATCAGCTATTTGCGTGTGAGGAAAC TTCTGGATGCTGGTGATTTGGATATTGAAGATGACATGAAAGCACAGATGAATTGCTTTT ATTTGAAAGCCTTGGATGGTTTTGTTATGGTTCTCACAGATGATGGTGACATGATTTACA TTTCTGATAATGTGAACAAATACATGGGATTAACTCAGTTTGAACTAACTGGACACAGTG TGTTTGATTTTACTCATCCATGTGACCATGAGGAAATGAGAGAAATGCTTACACACAGAA ATGGCCTTGTGAAAAAGGGTAAAGAACAAAACACACAGCGAAGCTTTTTTCTCAGAATGA AGTGTACCCTAACTAGCCGAGGAAGAACTATGAACATAAAGTCTGCAACATGGAAGGTAT TGCACTGCACAGGCCACATTCACGTATATGATACCAACAGTAACCAACCTCAGTGTGGGT ATAAGAAACCACCTATGACCTGCTTGGTGCTGATTTGTGAACCCATTCCTCACCCATCAA ATATTGAAATTCCTTTAGATAGCAAGACTTTCCTCAGTCGACACAGCCTGGATATGAAAT TTTCTTATTGTGATGAAAGAATTACCGAATTGATGGGATATGAGCCAGAAGAACTTTTAG GCCGCTCAATTTATGAATATTATCATGCTTTGGACTCTGATCATCTGACCAAAACTCATC ATGATATGTTTACTAAAGGACAAGTCACCACAGGACAGTACAGGATGCTTGCCAAAAGAG GTGGATATGTCTGGGTTGAAACTCAAGCAACTGTCATATATAACACCAAGAATTCTCAAC CACAGTGCATTGTATGTGTGAATTACGTTGTGAGTGGTATTATTCAGCACGACTTGATTT TCTCCCTTCAACAAACAGAATGTGTCCTTAAACCGGTTGAATCTTCAGATATGAAAATGA CTCAGCTATTCACCAAAGTTGAATCAGAAGATACAAGTAGCCTCTTTGACAAACTTAAGA AGGAACCTGATGCTTTAACTTTGCTGGCCCCAGCCGCTGGAGACACAATCATATCTTTAG ATTTTGGCAGCAACGACACAGAAACTGATGACCAGCAACTTGAGGAAGTACCATTATATA ATGATGTAATGCTCCCCTCACCCAACGAAAAATTACAGAATATAAATTTGGCAATGTCTC CATTACCCACCGCTGAAACGCCAAAGCCACTTCGAAGTAGTGCTGACCCTGCACTCAATC AAGAAGTTGCATTAAAATTAGAACCAAATCCAGAGTCACTGGAACTTTCTTTTACCATGC CCCAGATTCAGGATCAGACACCTAGTCCTTCCGATGGAAGCACTAGACAAAGTTCACCTG AGCCTAATAGTCCCAGTGAATATTGTTTTTATGTGGATAGTGATATGGTCAATGAATTCA AGTTGGAATTGGTAGAAAAACTTTTTGCTGAAGACACAGAAGCAAAGAACCCATTTTCTA CTCAGGACACAGATTTAGACTTGGAGATGTTAGCTCCCTATATCCCAATGGATGATGACT TCCAGTTACGTTCCTTCGATCAGTTGTCACCATTAGAAAGCAGTTCCGCAAGCCCTGAAA GCGCAAGTCCTCAAAGCACAGTTACAGTATTCCAGCAGACTCAAATACAAGAACCTACTG CTAATGCCACCACTACCACTGCCACCACTGATGAATTAAAAACAGTGACAAAAGACCGTA TGGAAGACATTAAAATATTGATTGCATCTCCATCTCCTACCCACATACATAAAGAAACTA CTAGTGCCACATCATCACCATATAGAGATACTCAAAGTCGGACAGCCTCACCAAACAGAG CAGGAAAAGGAGTCATAGAACAGACAGAAAAATCTCATCCAAGAAGCCCTAACGTGTTAT CTGTCGCTTTGAGTCAAAGAACTACAGTTCCTGAGGAAGAACTAAATCCAAAGATACTAG CTTTGCAGAATGCTCAGAGAAAGCGAAAAATGGAACATGATGGTTCACTTTTTCAAGCAG TAGGAATTGGAACATTATTACAGCAGCCAGACGATCATGCAGCTACTACATCACTTTCTT GGAAACGTGTAAAAGGATGCAAATCTAGTGAACAGAATGGAATGGAGCAAAAGACAATTA TTTTAATACCCTCTGATTTAGCATGTAGACTGCTGGGGCAATCAATGGATGAAAGTGGAT TACCACAGCTGACCAGTTATGATTGTGAAGTTAATGCTCCTATACAAGGCAGCAGAAACC TACTGCAGGGTGAAGAATTACTCAGAGCTTTGGATCAAGTTAACTGAGCTTTTTCTTAAT TTCATTCCTTTTTTTGGACACTGGTGGCTCACTACCTAAAGCAGTCTATTTATATTTTCT ACATCTAATTTTAGAAGCCTGGCTACAATACTGCACAAACTTGGTTAGTTCAATTTTTGA TCCCCTTTCTACTTAATTTACATTAATGCTCTTTTTTAGTATGTTCTTTAATGCTGGATC ACAGACAGCTCATTTTCTCAGTTTTTTGGTATTTAAACCATTGCATTGCAGTAGCATCAT TTXXXXXXXXXXXXCTCGAC >OriGene 5' read for NM_001530 unedited
AGTATTTTGTAATACGACTCACTTATAGGGCGGCCGCGAATCGGCACGAGGCCTCTTCGT CGCTTCGGCCAGTGTGTCGGGCTGGGCCCTGACAAGCCACCTGAGGAGAGGCTCGGAGCC GGGCCCGGACCCCGGCGATTGCCGCCCGCTTCTCTCTAGTCTCACGAGGGGTTTCCCGCC TCGCACCCCCACCTCTGGACTTGCCTTTCCTTCTCTTCTCCGCGTGTGGAGGGAGCCAGC GCTTAGGCCGGAGCGAGCCTGGGGGCCGCCCGCCGTGAAGACATCGCGGGGACCGATTCA CCATGGAGGGCGCCGGCGGCGCGAACGACAAGAAAAAGATAAGTTCTGAACGTCGAAAAG AAAAGTCTCGAGATGCAGCCAGATCTCGGCGAAGTAAAGAATCTGAAGTTTTTTATGAGC TTGCTCATCAGTTGCCACTTCCACATAATGTGAGTTCGCATCTTGATAAGGCCTCTGTGA TGAGGCTTACCATCAGCTATTTGCGTGTGAGGAAACTTCTGGATGCTGGTGATTTGGATA TTGAAGATGACATGAAAGCACAGATGAATTGCTTTTATTTGAAAGCCTTGGATGGTTTTG TTATGGTTCTCACAGATGATGGTGACATGATTTACATTTCTGATAATGTGAACAAATACA TGGGATTAACTCAGTTTGAACTAACTGGACACAGTGGTGTTTGATTTTACTCATCCATGT GACCATGAGGAAATGAGAGAAATGCTTACACACAGAAATGGCCTTGTGAAAAAGGGTANA GAACANAACACACAGCGAAGCTTTNTTCTCAGAATGAAGTGTACCCTAACTAGCCCGAGG AGAACTATGAACATAAAGTCTGCACCTTGGAAGGTATTGCACTGACAGGCCACATTCACG TATATGATACCACAGTACCACCTCCATGTGGGGTATAAGAACCACTAGACCGGCTGGGGC GCTGGATT >OriGene 3' read for NM_001530 unedited
CGCGGCCCGCATTCTAANATCGAGTTTTTTTTTTTTTTTTTTTTTTTTCCTGGTTTTAAA AAAGTTTATTTGATGGAACAAAACAATACAGTTAGTGTTAGATCCAACCACAAAGAGCAA AAGGAATGAAAAATTTGTACAATGTACATAGAAAAAAACAAGATATTTACTGTGACAACT ATATATTCCTAAAATAATGCTTCTAAATTTTTTCAATTATTGAAATCTACATGGGGAAAA GGATGTTAATAGCGACAAAGTGCATAAAATCAAACATTGTATTTTGAGCAAATTAACATA CTAGGCAATTTTGCTAAAAATGCATGATTTTTTTTTTCTTGTTTACAGCCTGCTCAAAAT ATCTTTATACCAACAGGGTAGGCACAACATTTAGGTTTAATATCAGTTACACAATATTAG CATAAACTTTCACAACTACATAGGGTATTGCCCTCTTTTGACCTGGCAAAGTGACTATAC AAACATTATATGATTCCTCTGAATTGACAATTTCATCCAAATAAATGCCACATACCTTGT ACATATATGCATATCTTCCTATATTATGCAAATGGCTTTACTCTTTAAAAATTAACCATG CATGATACAACAATCATTATCCTCCGATTAACACTGTGCTGCGGCAGCGTCAACACTCGT GCTGGCCCAAGAAAAACTTTCGAGCTAAAAACTGTTCTATCAACCCATAATATGTCTCAT GAGCCACTGTTGGTCTTCTCTAATAAAAACTCTTGTGCAATGGGGGCTACCACACCCTGT TGGCAAACGCCCTTATCTACGCACAAGGGGAAAAAAAGGGGGAAAAATTGCCAACGGCCT TTATTTTTATCAAAGTATTGGCCTATTAAAAAATATTTGGACTTTTCTAAAATATTCGAA AAGGATAACCTCCCTTCCAAAAAAATAAAAAGGGGCAT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_001530 |
| Insert Size | 4000 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001530.2, NP_001521.1 |
| RefSeq Size | 3958 bp |
| RefSeq ORF | 2481 bp |
| Locus ID | 3091 |
| UniProt ID | Q16665 |
| Domains | PAS, HLH, PAC |
| Protein Families | Transcription Factors |
| Protein Pathways | mTOR signaling pathway, Pathways in cancer, Renal cell carcinoma |
| Gene Summary | This gene encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in energy metabolism, angiogenesis, apoptosis, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2011] Transcript Variant: This variant (1) represents the predominant transcript, and encodes isoform 1. |
Citations (14)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Estrogen-related receptor α is involved in angiogenesis and skeletal muscle revascularization in hindlimb ischemia
,Sopariwala, DH;Likhite, N;Pei, G;Haroon, F;Lin, L;Yadav, V;Zhao, Z;Narkar, VA;,
FASEB journal : official publication of the Federation of American Societies for Experimental Biology
,PubMed ID 33788962
[HIF1A]
|
|
Midkine Is a Potential Therapeutic Target of Tumorigenesis, Angiogenesis, and Metastasis in Non-Small Cell Lung Cancer
,Shin, DH;Jo, JY;Kim, SH;Choi, M;Han, C;Choi, BK;Kim, SS;,
Cancers (Basel) 2020
,PubMed ID 32847073
[HIF1A]
|
|
Direct eNAMPT Involvement in Pulmonary Hypertension and Vascular Remodeling: Transcriptional Regulation by SOX and HIF2α
,Sun, X;Sun, BL;Babicheva, A;Vanderpool, R;Oita, RC;Casanova, N;Tang, H;Gupta, A;Lynn, H;Gupta, G;Rischard, F;Sammani, S;Kempf, CL;Moreno-Vinasco, L;Ahmed, M;Camp, SM;Wang, J;Desai, AA;Yuan, JX;Garcia, JGN;,
Am. J. Respir. Cell Mol. Biol.
,PubMed ID 32142369
[HIF1A]
|
|
Hypoxia-inducible factor-1 promotes cancer progression through activating AKT/Cyclin D1 signaling pathway in osteosarcoma.
,null,
Biomedicine pharmacotherapy = Biomedecine pharmacotherapie
,PubMed ID 29807229
[HIF1A]
|
|
Andrographolide inhibits the migration, invasion and matrix metalloproteinase expression of rheumatoid arthritis fibroblast-like synoviocytes via inhibition of HIF-1a signaling
,Li, GF;Qin, YH;Du, PQ;,
Life Sci.
,PubMed ID 26141990
[HIF1A]
|
|
Oroxylin A inhibits glycolysis-dependent proliferation of human breast cancer via promoting SIRT3-mediated SOD2 transcription and HIF1a destabilization
,Wei, L;Zhou, Y;Qiao, C;Ni, T;Li, Z;You, Q;Guo, Q;Lu, N;,
Cell Death Dis
,PubMed ID 25855962
[HIF1A]
|
|
Intermittent hypoxia effect on osteoclastogenesis stimulated by neuroblastoma cells
,Bhaskara, VK;Mohanam, I;Gujrati, M;Mohanam, S;,
PLoS ONE
,PubMed ID 25148040
[HIF1A]
|
|
Variations of CITED2 Are Associated with Congenital Heart Disease (CHD) in Chinese Population
,Liu, Y;Wang, F;Wu, Y;Tan, S;Wen, Q;Wang, J;Zhu, X;Wang, X;Li, C;Ma, X;Pan, H;,
PLoS ONE
,PubMed ID 24848765
[HIF1A]
|
|
Proline-Hydroxylated Hypoxia-Inducible Factor 1α (HIF-1α) Upregulation in Human Tumours
,Snell, CE;Turley, H;McIntyre, A;Li, D;Masiero, M;Schofield, CJ;Gatter, KC;Harris, AL;Pezzella, F;,
PLoS ONE, Feb 2014.
,PubMed ID 24563687
[HIF1A]
|
|
Hypoxia-induced invadopodia formation: a role for ß-PIX
,Nur Fariesha Md Hashim, Nicole S. Nicholas, Anna E. Dart, Serafim Kiriakidis, Ewa Paleolog, and Claire M. Wells,
Open Bio, Jun 2013; 3: 120159.
,PubMed ID 23740575
[HIF1A]
|
|
Regulation of the Bone-restricted IFITM-like ( Bril ) Gene Transcription by Sp and Gli Family Members and CpG Methylation
,null,
The Journal of Biological Chemistry
,PubMed ID 23530031
[HIF1A]
|
|
The Paradox of E-Cadherin: Role in response to hypoxia in the tumor microenvironment and regulation of energy metabolism
,null,
Oncotarget
,PubMed ID 23530113
[HIF1A]
|
|
Gene expression of the IGF pathway family distinguishes subsets of gastrointestinal stromal tumors wild type for KIT and PDGFRA
,null,
Cancer Medicine
,PubMed ID 24133624
[HIF1A]
|
|
Transglutaminase 2 protects against ischemic insult, interacts with HIF1{beta}, and attenuates HIF1 signaling
,Anthony J. Filiano, Craig D. C. Bailey, Janusz Tucholski, Soner Gundemir, and Gail V. W. Johnson,
FASEB J. published 28 March 2008, 10.1096/fj.07-097709
[HIF1A]
|
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202461 | HIF1A (Myc-DDK-tagged)-Human hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) (HIF1A), transcript variant 1 |
CNY 6,048.00 |
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| RC202461L1 | Lenti ORF clone of Human hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) (HIF1A), transcript variant 1, Myc-DDK-tagged |
CNY 8,448.00 |
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| RC202461L2 | Lenti ORF clone of Human hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) (HIF1A), transcript variant 1, mGFP tagged |
CNY 8,448.00 |
|
| RC202461L3 | Lenti ORF clone of Human hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) (HIF1A), transcript variant 1, Myc-DDK-tagged |
CNY 8,448.00 |
|
| RC202461L4 | Lenti ORF clone of Human hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) (HIF1A), transcript variant 1, mGFP tagged |
CNY 7,700.00 |
|
| RG202461 | HIF1A (tGFP-tagged) - Human hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) (HIF1A), transcript variant 1 |
CNY 7,648.00 |
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