A RAF (ARAF) (NM_001654) Human Untagged Clone

CAT#: SC119127

ARAF (untagged)-Human v-raf murine sarcoma 3611 viral oncogene homolog (ARAF)



  "NM_001654" in other vectors (7)

CNY 4,520.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
ARAF mouse monoclonal antibody, clone OTI2G9 (formerly 2G9)
    • 100 ul

CNY 1,999.00
CNY 2,700.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms A-RAF; ARAF1; PKS2; RAFA1
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF sequence for NM_001654 edited
ATGGAGCCACCACGGGGCCCCCCTGCCAATGGGGCCGAGCCATCCCGGGCAGTGGGCACC
GTCAAAGTATACCTGCCCAACAAGCAACGCACGGTGGTGACTGTCCGGGATGGCATGAGT
GTCTACGACTCTCTAGACAAGGCCCTGAAGGTGCGGGGTCTAAATCAGGACTGCTGTGTG
GTCTACCGACTCATCAAGGGACGAAAGACGGTCACTGCCTGGGACACAGCCATTGCTCCC
CTGGATGGCGAGGAGCTCATTGTCGAGGTCCTTGAAGATGTCCCGCTGACCATGCACAAT
TTTGTACGGAAGACCTTCTTCAGCCTGGCGTTCTGTGACTTCTGCCTTAAGTTTCTGTTC
CATGGCTTCCGTTGCCAAACCTGTGGCTACAAGTTCCACCAGCATTGTTCCTCCAAGGTC
CCCACAGTCTGTGTTGACATGAGTACCAACCGCCAACAGTTCTACCACAGTGTCCAGGAT
TTGTCCGGAGGCTCCAGACAGCATGAGGCTCCCTCGAACCGCCCCCTGAATGAGTTGCTA
ACCCCCCAGGGTCCCAGCCCCCGCACCCAGCACTGTGACCCGGAGCACTTCCCCTTCCCT
GCCCCAGCCAATGCCCCCCTACAGCGCATCCGCTCCACGTCCACTCCCAACGTCCATATG
GTCAGCACCACGGCCCCCATGGACTCCAACCTCATCCAGCTCACTGGCCAGAGTTTCAGC
ACTGATGCTGCCGGTAGTAGAGGAGGTAGTGATGGAACCCCCCGGGGGAGCCCCAGCCCA
GCCAGCGTGTCCTCGGGGAGGAAGTCCCCACATTCCAAGTCACCAGCAGAGCAGCGCGAG
CGGAAGTCCTTGGCCGATGACAAGAAGAAAGTGAAGAACCTGGGGTACCGGGACTCAGGC
TATTACTGGGAGGTACCACCCAGTGAGGTGCAGCTGCTGAAGAGGATCGGGACGGGCTCG
TTTGGCACCGTGTTTCGAGGGCGGTGGCATGGCGATGTGGCCGTGAAGGTGCTCAAGGTG
TCCCAGCCCACAGCTGAGCAGGCCCAGGCTTTCAAGAATGAGATGCAGGTGCTCAGGAAG
ACGCGACATGTCAACATCTTGCTGTTTATGGGCTTCATGACCCGGCCGGGATTTGCCATC
ATCACACAGTGGTGTGAGGGCTCCAGCCTCTACCATCACCTGCATGTGGCCGACACACGC
TTCGACATGGTCCAGCTCATCGACGTGGCCCGGCAGACTGCCCAGGGCATGGACTACCTC
CATGCCAAGAACATCATCCACCGAGATCTCAAGTCTAACAACATCTTCCTACATGAGGGG
CTCACGGTGAAGATCGGTGACTTTGGCTTGGCCACAGTGAAGACTCGATGGAGCGGGGCC
CAGCCCTTGGAGCAGCCCTCAGGATCTGTGCTGTGGATGGCAGCTGAGGTGATCCGTATG
CAGGACCCGAACCCCTACAGCTTCCAGTCAGACGTCTATGCCTACGGGGTTGTGCTCTAC
GAGCTTATGACTGGCTCACTGCCTTACAGCCACATTGGCTGCCGTGACCAGATTATCTTT
ATGGTGGGCCGTGGCTATCTGTCCCCGGACCTCAGCAAAATCTCCAGCAACTGCCCCAAG
GCCATGCGGCGCCTGCTGTCTGACTGCCTCAAGTTCCAGCGGGAGGAGCGGCCCCTCTTC
CCCCAGATCCTGGCCACAATTGAGCTGCTGCAACGGTCACTCCCCAAGATTGAGCGGAGT
GCCTCGGAACCCTCCTTGCACCGCACCCAGGCCGATGAGTTGCCTGCCTGCCTACTCAGC
GCAGCCCGCCTTGTGCCTTAG
>OriGene 5' read for NM_001654 unedited
GGTTTACAATTGTAAACGACTCATATAGGCGGCCGCGAAATTCGCACGAAGGCCAAAATG
GAGACGGCGGCGGCTGTAGCGGCGTGACAGGAGCCCCATGGCACCTGCCCAGCCCCACCT
CAGCCCATCTTGACAAAATCTAAGGCTCCATGGAGCCACCACGGGGCCCCCCTGCCAATG
GGGCCGAGCCATCCCGGGCAGTGGGCACCGTCAAAGTATACCTGCCCAACAAGCAACGCA
CGGTGGTGACTGTCCGGGATGGCATGAGTGTCTACGACTCTCTAGACAAGGCCCTGAAGG
TGCGGGGTCTAAATCAGGACTGCTGTGTGGTCTACCGACTCATCAAGGGACGAAAGACGG
TCACTGCCTGGGACACAGCCATTGCTCCCCTGGATGGCGAGGAGCTCATTGTCGAGGTCC
TTGAAGATGTCCCGCTGACCATGCACAATTTTGTACGGAAGACCTTCTTCAGCCTGGCGT
TCTGTGACTTCTGCCTTAAGTTTCTGTTCCATGGCTTCCGTTGCCAAACCTGTGGCTACA
AGTTCCACCAGCATTGTTCCTCCAAGGTCCCCACAGTCTGTGTTGACATGAGTACCAACC
GCCAACAGTTCTACCACAGTGTCCAGGATTTGTCCGGAGGCTCCAGACAGCATGAGGCTC
CCTCGAACCGCCCCCTGAATGAGTTGCTAACCCCCCCAGGTCCCAGNCCCCGCACCCAGC
ACTGTGACCCGNAGCACTTNCCCCTTCCTGCCCCAACCAATGCCCCCCCTACAGCGCATC
CGCTCCACGTCCACTCCCCACGTCCATATGGTCAACACCACGGCCCCCATGGACTCCAAC
TCATNCAGCTCACTGGCCAAAGTTTCANCACTGATGCTNGCCGTAGTANAAGAAGTANTG
ATGGAAACCCCCCGG
>OriGene 3' read for NM_001654 unedited
CGGCACGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCCAGCAGAATTCTGTGCTTC
TTGGTGAAGAAAGAGAGGAGTCCTTGAATGGCGGGGGAGACACAAAAGGGACCCCCCAAA
ATCCACATGGGGTGCTGGGAACCCCAAAATCCAGTGGAAGGCACATCAGGCACAAATTCC
AGAGAGGTTCTGAGTGGAATCCCTGCCACAAATCCCAGCCATTGGAGATGGAGGAGCTCC
CAAAATTTAAAAGTATCCCCAAAGCCAAGAGGAAACCAAATGATGGAGGAGACAGGGGGC
TCAGTCTTTGGCGGGGGTCCCCCAATTCCAGAAGAACTGGAAAAGCACATGGGGACCCCC
TCATCTCCCAGGGTGGGGAATGGGGGATCCTGAGGCAGCATCAGGGCAGAGACGAACATT
GATTGGCTGGTGGGCAAGGCTCCTTGGCGTGGAGGGCTGAAATTGGCTCCCTGGTGGCTT
GGGCGGGGCCTAAGGCACAAGGCGGGCTGCGCTGAGTAGGCAGGCAGGCAACTCATCGGC
CTGNGTGCGGTGCAAGGAGGGTTCCGAGGCACTCCGCTCAATCTTGGGGAGTGACCGTTG
CAGCAGCTCAATTGTGGCCAGGATCTGGGGGAAGAGGGGCCGCTCCTCCCGCTGGAACTT
GAGGCANTCANACAGCANGCGCCGCATGGCCTTGGGGCANNTGCTGGAGATTTTGCCTGA
GTCGGGGACAGATACCCCGGCCCACATAAAGATATCTGGTCACGCACCCATGTGGCTGTA
GGCANTGAGCCANTCATACCTCCTANACCCACCCCCGTAGCTANACTCTGACTGGAACTT
AAGGGGTCCGGTCCTGCTTACGATCACCTANCTGCCTCCCAGCAAAATCTGGAGGCTGCT
CAAGGCTGGCCCCCCCTCTTGAGTCTCCTGTGCCAACAAAAACACGTCTTACCGGG
Restriction Sites NotI-NotI     
ACCN NM_001654
Insert Size 2380 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001654.1, NP_001645.1
RefSeq Size 2466 bp
RefSeq ORF 1821 bp
Locus ID 369
UniProt ID P10398
Domains pkinase, TyrKc, DAG_PE-bind, S_TKc, RBD
Protein Families Druggable Genome, Protein Kinase
Protein Pathways Acute myeloid leukemia, Bladder cancer, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Glioma, Insulin signaling pathway, Long-term depression, Long-term potentiation, Melanoma, Natural killer cell mediated cytotoxicity, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Progesterone-mediated oocyte maturation, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma, Vascular smooth muscle contraction
Gene Summary This proto-oncogene belongs to the RAF subfamily of the Ser/Thr protein kinase family, and maybe involved in cell growth and development. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2012]
Transcript Variant: This variant (1) encodes the predominant isoform (1).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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