A RAF (ARAF) (NM_001654) Human Untagged Clone
CAT#: SC119127
ARAF (untagged)-Human v-raf murine sarcoma 3611 viral oncogene homolog (ARAF)
CNY 4,520.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | A-RAF; ARAF1; PKS2; RAFA1 |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF sequence for NM_001654 edited
ATGGAGCCACCACGGGGCCCCCCTGCCAATGGGGCCGAGCCATCCCGGGCAGTGGGCACC GTCAAAGTATACCTGCCCAACAAGCAACGCACGGTGGTGACTGTCCGGGATGGCATGAGT GTCTACGACTCTCTAGACAAGGCCCTGAAGGTGCGGGGTCTAAATCAGGACTGCTGTGTG GTCTACCGACTCATCAAGGGACGAAAGACGGTCACTGCCTGGGACACAGCCATTGCTCCC CTGGATGGCGAGGAGCTCATTGTCGAGGTCCTTGAAGATGTCCCGCTGACCATGCACAAT TTTGTACGGAAGACCTTCTTCAGCCTGGCGTTCTGTGACTTCTGCCTTAAGTTTCTGTTC CATGGCTTCCGTTGCCAAACCTGTGGCTACAAGTTCCACCAGCATTGTTCCTCCAAGGTC CCCACAGTCTGTGTTGACATGAGTACCAACCGCCAACAGTTCTACCACAGTGTCCAGGAT TTGTCCGGAGGCTCCAGACAGCATGAGGCTCCCTCGAACCGCCCCCTGAATGAGTTGCTA ACCCCCCAGGGTCCCAGCCCCCGCACCCAGCACTGTGACCCGGAGCACTTCCCCTTCCCT GCCCCAGCCAATGCCCCCCTACAGCGCATCCGCTCCACGTCCACTCCCAACGTCCATATG GTCAGCACCACGGCCCCCATGGACTCCAACCTCATCCAGCTCACTGGCCAGAGTTTCAGC ACTGATGCTGCCGGTAGTAGAGGAGGTAGTGATGGAACCCCCCGGGGGAGCCCCAGCCCA GCCAGCGTGTCCTCGGGGAGGAAGTCCCCACATTCCAAGTCACCAGCAGAGCAGCGCGAG CGGAAGTCCTTGGCCGATGACAAGAAGAAAGTGAAGAACCTGGGGTACCGGGACTCAGGC TATTACTGGGAGGTACCACCCAGTGAGGTGCAGCTGCTGAAGAGGATCGGGACGGGCTCG TTTGGCACCGTGTTTCGAGGGCGGTGGCATGGCGATGTGGCCGTGAAGGTGCTCAAGGTG TCCCAGCCCACAGCTGAGCAGGCCCAGGCTTTCAAGAATGAGATGCAGGTGCTCAGGAAG ACGCGACATGTCAACATCTTGCTGTTTATGGGCTTCATGACCCGGCCGGGATTTGCCATC ATCACACAGTGGTGTGAGGGCTCCAGCCTCTACCATCACCTGCATGTGGCCGACACACGC TTCGACATGGTCCAGCTCATCGACGTGGCCCGGCAGACTGCCCAGGGCATGGACTACCTC CATGCCAAGAACATCATCCACCGAGATCTCAAGTCTAACAACATCTTCCTACATGAGGGG CTCACGGTGAAGATCGGTGACTTTGGCTTGGCCACAGTGAAGACTCGATGGAGCGGGGCC CAGCCCTTGGAGCAGCCCTCAGGATCTGTGCTGTGGATGGCAGCTGAGGTGATCCGTATG CAGGACCCGAACCCCTACAGCTTCCAGTCAGACGTCTATGCCTACGGGGTTGTGCTCTAC GAGCTTATGACTGGCTCACTGCCTTACAGCCACATTGGCTGCCGTGACCAGATTATCTTT ATGGTGGGCCGTGGCTATCTGTCCCCGGACCTCAGCAAAATCTCCAGCAACTGCCCCAAG GCCATGCGGCGCCTGCTGTCTGACTGCCTCAAGTTCCAGCGGGAGGAGCGGCCCCTCTTC CCCCAGATCCTGGCCACAATTGAGCTGCTGCAACGGTCACTCCCCAAGATTGAGCGGAGT GCCTCGGAACCCTCCTTGCACCGCACCCAGGCCGATGAGTTGCCTGCCTGCCTACTCAGC GCAGCCCGCCTTGTGCCTTAG >OriGene 5' read for NM_001654 unedited
GGTTTACAATTGTAAACGACTCATATAGGCGGCCGCGAAATTCGCACGAAGGCCAAAATG GAGACGGCGGCGGCTGTAGCGGCGTGACAGGAGCCCCATGGCACCTGCCCAGCCCCACCT CAGCCCATCTTGACAAAATCTAAGGCTCCATGGAGCCACCACGGGGCCCCCCTGCCAATG GGGCCGAGCCATCCCGGGCAGTGGGCACCGTCAAAGTATACCTGCCCAACAAGCAACGCA CGGTGGTGACTGTCCGGGATGGCATGAGTGTCTACGACTCTCTAGACAAGGCCCTGAAGG TGCGGGGTCTAAATCAGGACTGCTGTGTGGTCTACCGACTCATCAAGGGACGAAAGACGG TCACTGCCTGGGACACAGCCATTGCTCCCCTGGATGGCGAGGAGCTCATTGTCGAGGTCC TTGAAGATGTCCCGCTGACCATGCACAATTTTGTACGGAAGACCTTCTTCAGCCTGGCGT TCTGTGACTTCTGCCTTAAGTTTCTGTTCCATGGCTTCCGTTGCCAAACCTGTGGCTACA AGTTCCACCAGCATTGTTCCTCCAAGGTCCCCACAGTCTGTGTTGACATGAGTACCAACC GCCAACAGTTCTACCACAGTGTCCAGGATTTGTCCGGAGGCTCCAGACAGCATGAGGCTC CCTCGAACCGCCCCCTGAATGAGTTGCTAACCCCCCCAGGTCCCAGNCCCCGCACCCAGC ACTGTGACCCGNAGCACTTNCCCCTTCCTGCCCCAACCAATGCCCCCCCTACAGCGCATC CGCTCCACGTCCACTCCCCACGTCCATATGGTCAACACCACGGCCCCCATGGACTCCAAC TCATNCAGCTCACTGGCCAAAGTTTCANCACTGATGCTNGCCGTAGTANAAGAAGTANTG ATGGAAACCCCCCGG >OriGene 3' read for NM_001654 unedited
CGGCACGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCCAGCAGAATTCTGTGCTTC TTGGTGAAGAAAGAGAGGAGTCCTTGAATGGCGGGGGAGACACAAAAGGGACCCCCCAAA ATCCACATGGGGTGCTGGGAACCCCAAAATCCAGTGGAAGGCACATCAGGCACAAATTCC AGAGAGGTTCTGAGTGGAATCCCTGCCACAAATCCCAGCCATTGGAGATGGAGGAGCTCC CAAAATTTAAAAGTATCCCCAAAGCCAAGAGGAAACCAAATGATGGAGGAGACAGGGGGC TCAGTCTTTGGCGGGGGTCCCCCAATTCCAGAAGAACTGGAAAAGCACATGGGGACCCCC TCATCTCCCAGGGTGGGGAATGGGGGATCCTGAGGCAGCATCAGGGCAGAGACGAACATT GATTGGCTGGTGGGCAAGGCTCCTTGGCGTGGAGGGCTGAAATTGGCTCCCTGGTGGCTT GGGCGGGGCCTAAGGCACAAGGCGGGCTGCGCTGAGTAGGCAGGCAGGCAACTCATCGGC CTGNGTGCGGTGCAAGGAGGGTTCCGAGGCACTCCGCTCAATCTTGGGGAGTGACCGTTG CAGCAGCTCAATTGTGGCCAGGATCTGGGGGAAGAGGGGCCGCTCCTCCCGCTGGAACTT GAGGCANTCANACAGCANGCGCCGCATGGCCTTGGGGCANNTGCTGGAGATTTTGCCTGA GTCGGGGACAGATACCCCGGCCCACATAAAGATATCTGGTCACGCACCCATGTGGCTGTA GGCANTGAGCCANTCATACCTCCTANACCCACCCCCGTAGCTANACTCTGACTGGAACTT AAGGGGTCCGGTCCTGCTTACGATCACCTANCTGCCTCCCAGCAAAATCTGGAGGCTGCT CAAGGCTGGCCCCCCCTCTTGAGTCTCCTGTGCCAACAAAAACACGTCTTACCGGG |
Restriction Sites | NotI-NotI |
ACCN | NM_001654 |
Insert Size | 2380 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_001654.1, NP_001645.1 |
RefSeq Size | 2466 bp |
RefSeq ORF | 1821 bp |
Locus ID | 369 |
UniProt ID | P10398 |
Domains | pkinase, TyrKc, DAG_PE-bind, S_TKc, RBD |
Protein Families | Druggable Genome, Protein Kinase |
Protein Pathways | Acute myeloid leukemia, Bladder cancer, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Glioma, Insulin signaling pathway, Long-term depression, Long-term potentiation, Melanoma, Natural killer cell mediated cytotoxicity, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Progesterone-mediated oocyte maturation, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma, Vascular smooth muscle contraction |
Gene Summary | This proto-oncogene belongs to the RAF subfamily of the Ser/Thr protein kinase family, and maybe involved in cell growth and development. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2012] Transcript Variant: This variant (1) encodes the predominant isoform (1). |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC200737 | ARAF (Myc-DDK-tagged)-Human v-raf murine sarcoma 3611 viral oncogene homolog (ARAF) |
CNY 4,512.00 |
|
RC200737L1 | Lenti ORF clone of Human v-raf murine sarcoma 3611 viral oncogene homolog (ARAF), Myc-DDK-tagged |
CNY 6,912.00 |
|
RC200737L2 | Lenti ORF clone of Human v-raf murine sarcoma 3611 viral oncogene homolog (ARAF), mGFP tagged |
CNY 5,890.00 |
|
RC200737L3 | Lenti ORF clone of Human v-raf murine sarcoma 3611 viral oncogene homolog (ARAF), Myc-DDK-tagged |
CNY 6,912.00 |
|
RC200737L4 | Lenti ORF clone of Human v-raf murine sarcoma 3611 viral oncogene homolog (ARAF), mGFP tagged |
CNY 6,912.00 |
|
RG200737 | ARAF (tGFP-tagged) - Human v-raf murine sarcoma 3611 viral oncogene homolog (ARAF) |
CNY 6,112.00 |
|
SC323512 | ARAF (untagged)-Kinase deficient mutant (K336M) of Human v-raf murine sarcoma 3611 viral oncogene homolog (ARAF) |
CNY 4,520.00 |