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EIF4E (NM_001968) Human Untagged Clone
CAT＃: SC118908
EIF4E (untagged)-Human eukaryotic translation initiation factor 4E (EIF4E), transcript variant 1
CNY 2,400.00
CNY 2,950.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | AUTS19; CBP; eIF-4E; EIF4E1; EIF4EL1; EIF4F |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC118908 sequence for NM_001968 edited (data generated by NextGen Sequencing)
ATGGCGACTGTCGAACCGGAAACCACCCCTACTCCTAATCCCCCGACTACAGAAGAGGAG AAAACGGAATCTAATCAGGAGGTTGCTAACCCAGAACACTATATTAAACATCCCCTACAG AACAGATGGGCACTCTGGTTTTTTAAAAATGATAAAAGCAAAACTTGGCAAGCAAACCTG CGGCTGATCTCCAAGTTTGATACTGTTGAAGACTTTTGGGCTCTGTACAACCATATCCAG TTGTCTAGTAATTTAATGCCTGGCTGTGACTACTCACTTTTTAAGGATGGTATTGAGCCT ATGTGGGAAGATGAGAAAAACAAACGGGGAGGACGATGGCTAATTACATTGAACAAACAG CAGAGACGAAGTGACCTCGATCGCTTTTGGCTAGAGACACTTCTGTGCCTTATTGGAGAA TCTTTTGATGACTACAGTGATGATGTATGTGGCGCTGTTGTTAATGTTAGAGCTAAAGGT GATAAGATAGCAATATGGACTACTGAATGTGAAAACAGAGAAGCTGTTACACATATAGGG AGGGTATACAAGGAAAGGTTAGGACTTCCTCCAAAGATAGTGATTGGTTATCAGTCCCAC GCAGACACAGCTACTAAGAGCGGCTCCACCACTAAAAATAGGTTTGTTGTTTAA Clone variation with respect to NM_001968.3 >OriGene 5' read for NM_001968 unedited
GCAATTGTATACGACTCACTATAGGGCGGCCGCGAAATTCGCACGAGGTCTGGAGCGGTT GTGCGATCAGATCGATCTAAGATGGCGACTGTCGAACCGGAAACCACCCCTACTCCTAAT CCCCCGACTACAGAAGAGGAGAAAACGGAATCTAATCAGGAGGTTGCTAACCCAGAACAC TATATTAAACATCCCCTACAGAACAGATGGGCACTCTGGTTTTTTAAAAATGATAAAAGC AAAACTTGGCAAGCAAACCTGCGGCTGATCTCCAAGTTTGATACTGTTGAAGACTTTTGG GCTCTGTACAACCATATCCAGTTGTCTAGTAATTTAATGCCTGGCTGTGACTACTCACTT TTTAAGGATGGTATTGAGCCTATGTGGGAAGATGAGAAAAACAAACGGGGAGGACGATGG CTAATTACATTGAACAAACAGCAGAGACGAAGTGACCTCGATCGCTTTTGGCTAGAGACA CTTCTGTGCCTTATTGGAGAATCTTTTGATGACTACAGTGATGATGTATGTGGCGCTGTT GTTAATGTTAGAGCTAAAGGTGATAAGATAGCAATATGGACTACTGAATGTGAAAACAGA GAAGCTGTTACACATATAGGGAGGGTATACAAGGAAAGGTTAGGACTTCCTCCAAAGATA GTGATTGGTTATCAGTCCCACGCAGACACAGCTACTAAGAGCGGCTCCACCACTAAAAAT AGGGTTTGTTGTTTAAGAAGACACCTTCTGAGTATTCTCATAGGAGACTGCGTCAAGCAA TCGAGATTTGGGAGCTGAACCAAAGCCTCTTCAAAAAGCAGAGTGGACTGCATTTAAAAT TGATTTCCATCTTAATGGTACTCAGATATAAGAGAAGTCTCATTCGCCTTTGNNCTGNAC TCTGNGTCATTTTTTTTTTTTTTTGGCTANATTNCCCTTNCCATCAAGAATACAGTCCAT CCCAGATCTAATGTGTTCTGCCACTTGNATA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_001968 |
| Insert Size | 2500 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001968.2, NP_001959.1 |
| RefSeq Size | 2493 bp |
| RefSeq ORF | 654 bp |
| Locus ID | 1977 |
| UniProt ID | P06730 |
| Domains | IF4E |
| Protein Pathways | Insulin signaling pathway, mTOR signaling pathway |
| Gene Summary | The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of messenger RNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the 5'-cap structure. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. This gene acts as a proto-oncogene, and its expression and activation is associated with transformation and tumorigenesis. Several pseudogenes of this gene are found on other chromosomes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015] Transcript Variant: This variant (1) encodes the shortest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because transcript sequence consistent with the reference genome assembly was not available for all regions of the RefSeq transcript. The extent of this transcript is supported by transcript alignments. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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Phosphorylation independent eIF4E translational reprogramming of selective mRNAs determines tamoxifen resistance in breast cancer
,Gong, C;Tsoi, H;Mok, KC;Cheung, J;Man, EPS;Fujino, K;Wong, A;Lam, EWF;Khoo, US;,
Oncogene
,PubMed ID 32066877
[EIF4E]
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Other Versions
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| RC207333L1 | Lenti ORF clone of Human eukaryotic translation initiation factor 4E (EIF4E), transcript variant 1, Myc-DDK-tagged |
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| RC207333L2 | Lenti ORF clone of Human eukaryotic translation initiation factor 4E (EIF4E), transcript variant 1, mGFP tagged |
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| RC207333L3 | Lenti ORF clone of Human eukaryotic translation initiation factor 4E (EIF4E), transcript variant 1, Myc-DDK-tagged |
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| RC207333L4 | Lenti ORF clone of Human eukaryotic translation initiation factor 4E (EIF4E), transcript variant 1, mGFP tagged |
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| RG207333 | EIF4E (tGFP-tagged) - Human eukaryotic translation initiation factor 4E (EIF4E), transcript variant 1 |
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