EEF1A2 (NM_001958) Human Untagged Clone

CAT#: SC118902

EEF1A2 (untagged)-Human eukaryotic translation elongation factor 1 alpha 2 (EEF1A2)



  "NM_001958" in other vectors (6)

CNY 5,488.00


货期*
现货

规格
    • 10 ug

Cited in 2 publications.

Product images

经常一起买 (3)
EEF1A2 Rabbit polyclonal Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

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TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms DEE33; EEF1AL; EF-1-alpha-2; EF1A; EIEE33; HS1; MRD38; STN; STNL
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC118902 sequence for NM_001958 edited (data generated by NextGen Sequencing)
ATGGGCAAGGAGAAGACCCACATCAACATCGTGGTCATCGGCCACGTGGACTCCGGAAAG
TCCACCACCACGGGCCACCTCATCTACAAATGCGGAGGTATTGACAAAAGGACCATTGAG
AAGTTCGAGAAGGAGGCGGCTGAGATGGGGAAGGGATCCTTCAAGTATGCCTGGGTGCTG
GACAAGCTGAAGGCGGAGCGTGAGCGCGGCATCACCATCGACATCTCCCTCTGGAAGTTC
GAGACCACCAAGTACTACATCACCATCATCGATGCCCCCGGCCACCGCGACTTCATCAAG
AACATGATCACGGGTACATCCCAGGCGGACTGCGCAGTGCTGATCGTGGCGGCGGGCGTG
GGCGAGTTCGAGGCGGGCATCTCCAAGAATGGGCAGACGCGGGAGCATGCCCTGCTGGCC
TACACGCTGGGTGTGAAGCAGCTCATCGTGGGCGTGAACAAAATGGACTCCACAGAGCCG
GCCTACAGCGAGAAGCGCTACGACGAGATCGTCAAGGAAGTCAGCGCCTACATCAAGAAG
ATCGGCTACAACCCGGCCACCGTGCCCTTTGTGCCCATCTCCGGCTGGCACGGTGACAAC
ATGCTGGAGCCCTCCCCCAACATGCCGTGGTTCAAGGGCTGGAAGGTGGAGCGTAAGGAG
GGCAACGCAAGCGGCGTGTCCCTGCTGGAGGCCCTGGACACCATCCTGCCCCCCACGCGC
CCCACGGACAAGCCCCTGCGCCTGCCGCTGCAGGACGTGTACAAGATTGGCGGCATTGGC
ACGGTGCCCGTGGGCCGGGTGGAGACCGGCATCCTGCGGCCGGGCATGGTGGTGACCTTT
GCGCCAGTGAACATCACCACTGAGGTGAAGTCAGTGGAGATGCACCACGAGGCTCTGAGC
GAAGCTCTGCCCGGCGACAACGTCGGCTTCAATGTGAAGAACGTGTCGGTGAAGGACATC
CGGCGGGGCAACGTGTGTGGGGACAGCAAGTCTGACCCGCCGCAGGAGGCTGCTCAGTTC
ACCTCCCAGGTCATCATCCTGAACCACCCGGGGCAGATTAGCGCCGGCTACTCCCCGGTC
ATCGACTGCCACACAGCCCACATCGCCTGCAAGTTTGCGGAGCTGAAGGAGAAGATTGAC
CGGCGCTCTGGCAAGAAGCTGGAGGACAACCCCAAGTCCCTGAAGTCTGGAGACGCGGCC
ATCGTGGAGATGGTGCCGGGAAAGCCCATGTGTGTGGAGAGCTTCTCCCAGTACCCGCCT
CTCGGCCGCTTCGCCGTGCGCGACATGAGGCAGACGGTGGCCGTAGGCGTCATCAAGAAC
GTGGAGAAGAAGAGCGGCGGCGCCGGCAAGGTCACCAAGTCGGCGCAGAAGGCGCAGAAG
GCGGGCAAGTGA

Clone variation with respect to NM_001958.2
>OriGene 5' read for NM_001958 unedited
ACCTGGATTTGTAAACCGNACTTCACTATAGGGCGGCACAAGCAATTCGCACGAGGGAGA
CCTCGGCTCCGGNAATCACTGCAGCCCCCCTCGCCCTGAGCCAGAGCACCCCGGGTCCCG
CCAGCCCCTCACACTCCCAGCAAAATGGGCAAGGAGAAGACCCACATCAACATCGTGGTC
ATCGGCCACGTGGACTCCGGAAAGTCCACCACCACGGTTTTCCTCATCTACAAATGCGGA
GGTATTGACAAAAGGACCATTGAGAAGTTCGAGAAGGAGGCGGCTGAGATGGGGAAGGGA
TCCTTCAAGTATGCCTGGGTGCTGGACAAGCTGAAGGCGGAGCGTGAGCGCGGCATCACC
ATCGACATCTCCCTCTGGAAGTTCGAGACCACCAAGTACTACATCACCATCATCGATGCC
CCCGGCCACCGCGACTTCATCAAGAACATGATCACGGGTACATCCCAGGCGGACTGCGCA
GTGCTGATCGTGGCGGCGGGCGTGGGCGAGTTCGAGGCGGGCATCTCCAAGAATGGGCAG
ACGCGGGAGCATGCCCTGCTGGCCTACACGCTGGGTGTGAAGCAGCTCATCGTGGGCGTG
AACAAAATGGACTCCACAGAGCCGGCCTACAGCGAGAAGCGCTACGACGAGATCGTCAAG
GAAGTCAGCGCCTACATCAAGAAGATCGGCTACAACCCGGCCACCGTGCCCTTTGTGCCC
CTCTCCGGCTGGCACGGTGACAACATGCTGGAGCCCTCCCCCACATGCCGTGGTTCAAGG
GCTGGAAGGTGGAGCGTAAGGAGGGCAACGCAANGCGCGTGTCCCTGCTGGNAGCCCTGG
ACACATNCTGCCCCCCACGCGCCCACGGACAGCCCCTGCGCCTGCCGCTGCAAAACGTGT
ACAAAATTGGCGGCATTGGCACCGTG
>OriGene 3' read for NM_001958 unedited
GGCGGTCACAGTGGAGCCACGCCGTCTCTGTTCAGGAAACAGCTATGACCGCGGCCGCAA
TCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAGGGTCTCTCACTTTATTGGGAAAACACAC
ACTGATCGTGGCGAGCCCTGGCCCCGGAACCCTGGGGGGGGCGCGCCTTTCCCCGTGGAC
AACCTCCACTGTCCCATTGCGGCGCAGCCGGGGATCGGTGAGCCGTGGCCTCTGGCGCTC
CACTGGCCCGACTTCCGCGCCTTTTGCGTCGCACTTGCTGACCTTGCCGGCGCCGCCGCT
CTTCTTCTCCACGTTCTTGATGACGCCTACGGCCACCGTCTGCCTTATGCTCCGCACTGT
TAATCGGCCGATAAGCGGGTACTGGGAGAAGCTCCTCCCACACATGGTCCTTTCCCGGCA
CCATCTCCACGATGGCCGTGTCTCCAGACTTCAGGGACTTGGTGTTGCCCCCCCTCTTCT
TGTCCGAGCGCCGTGTAATCTTCTCCTTCTCCTCCGCCAACTTGCATGCCACTTTGTTCG
GCGTGGTAGTCGATGACCGGGGAGTAGCCGGCGCCACCCTTGTCCCGGGCGGGTCAAGGA
TGATGGACCTGNGAGGTGAACCGAATCATCCTCCTGTTTCTGGGCACAACTTGCCGGGCT
CCTTTTACTTTGGCCCCGCCGGGGCGTCCCTCACCCGAACCGGTTCTTTATATTTGAAAC
CCACCCTTTTNTTCTTGTAAAAGCTTCGCTTCAAAGCCCCCGGGTGTCTCGGCCCGGGCT
AGCCCCGTGATGGTGTAGTTCTATTGCGCCCTTAGGCCCCCNCCCGCCCCCGGGCCTTTA
GATGCCCGTTCNTCCACTCGGCGCCTGTGTGCCCTGGCCCATACCACCCCACTCTTTTTA
AAGATACTGGAAAGAGAAGAGAGAG
Restriction Sites NotI-NotI     
ACCN NM_001958
Insert Size 1890 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001958.2, NP_001949.1
RefSeq Size 1841 bp
RefSeq ORF 1392 bp
Locus ID 1917
UniProt ID Q05639
Domains GTP_EFTU, GTP_EFTU_D3, GTP_EFTU_D2
Protein Families Druggable Genome
Gene Summary This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 2) is expressed in brain, heart and skeletal muscle, and the other isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas. This gene may be critical in the development of ovarian cancer. [provided by RefSeq, Mar 2014]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (2)

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