TFIIE beta (GTF2E2) (NM_002095) Human Untagged Clone

CAT#: SC118828

GTF2E2 (untagged)-Human general transcription factor IIE, polypeptide 2, beta 34kDa (GTF2E2)



  "NM_002095" in other vectors (5)

CNY 3,872.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
Rabbit polyclonal anti-TF2E2 antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms FE; TF2E2; TFIIE-B; TTD6
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_002095, the custom clone sequence may differ by one or more nucleotides


ATGGATCCAAGCCTGTTGAGAGAAAGGGAGCTGTTCAAAAAACGAGCTCTTTCTACTCCTGTAGTAGAAA
AACGTTCAGCATCTTCTGAGTCATCATCATCATCGTCAAAGAAGAAGAAAACAAAGGTAGAACATGGAGG
ATCGTCAGGCTCTAAACAAAATTCTGATCATAGCAATGGATCATTTAACTTGAAAGCTTTGTCAGGAAGC
TCTGGATATAAGTTTGGTGTTCTTGCTAAGATTGTGAATTACATGAAGACACGGCATCAGCGAGGAGATA
CGCATCCTCTAACCTTAGATGAAATTTTGGATGAAACACAACATTTAGATATTGGACTCAAGCAGAAACA
ATGGCTAATGACTGAGGCTTTAGTCAACAATCCCAAAATTGAAGTAATAGATGGGAAGTATGCTTTCAAG
CCCAAGTACAACGTGAGAGATAAGAAGGCCCTACTTAGGCTCTTAGATCAGCATGACCAGCGAGGATTAG
GAGGAATTCTTTTAGAAGACATAGAAGAAGCACTGCCCAATTCCCAGAAAGCTGTCAAGGCTTTGGGGGA
CCAGATACTATTTGTAAATCGTCCCGATAAGAAGAAAATACTTTTCTTCAATGATAAGAGCTGTCAGTTT
TCTGTGGATGAAGAATTTCAGAAACTGTGGAGGAGTGTCACTGTAGATTCCATGGACGAGGAGAAAATTG
AAGAATATCTGAAGCGACAGGGTATTTCTTCCATGCAGGAATCTGGACCAAAGAAAGTGGCCCCTATTCA
GAGAAGGAAAAAGCCTGCTTCACAGAAAAAGCGACGCTTTAAGACTCATAACGAACACTTGGCTGGAGTG
CTGAAGGATTACTCTGACATTACTTCCAGCAAATAG


>OriGene 5' read for NM_002095 unedited
TATATTTGTATACGACTTACTATAGGGCGGCCGCGAATTCGGCACGAGGCTAGCACCCGA
TCGGGAAGTGGCGGGCGGAGTCCCGGGTCCAGTCGCCGCCTCAGCTACCGCCGCTGCCGC
CGCCGCCGCCGCCACCGCCAGTGGTGAGACCCCGACCTGGCGGGTCAGCGCTGGGCGTGC
GTGCGGGCAGGCGGGGGCGCTGACGAGAAGCAGGAAGAGGGTGCAGTGCCGGCGTGGGCG
GCCGGCCGAGGCGGAGGCGCAGGAAGGGGGCGGCGAGTCGTGCGAGGCTGCCCTTCTCAC
TCAGCATTATGGATCCAAGCCTGTTGAGAGAAAGGGAGCTGTTCAAAAAACGAGCTCTTT
CTACTCCTGTAGTAGAAAAACGTTCAGCATCTTCTGAGTCATCATCATCATCGTCAAAGA
AGAAGAAAACAAAGGTAGAACATGGAGGATCGTCAGGCTCTAAACAAAATTCTGATCATA
GCAATGGATCATTTAACTTGAAAGCTTTGTCAGGAAGCTCTGGATATAAGTTTGGTGTTC
TTGCTAAGATTGTGAATTACATGAAGACACGGCATCAGCGAGGAGATACGCATCCTCTAA
CCTTAGATGAAATTTTGGATGAAACACAACATTTAGATATTGGACTCAAGCAGAAACAAT
GGCTAATGACTGAGGCTTTAGTCAACAATCCCAAAATTGAAGTAATAGATGGGAAGTATG
CTTTCAAGCCCAAGTACAACGTGAGAGATAAGAAGGCCCTACTTANGCTCTTAGATCAGC
ATGACCAGCGAGGATTAGGGAGGATTCTTTTAGAAGACATAGAAGAAGCACTGCCCAATT
CCCAGAAGCTGTCAGGGCTTTGGGGACAGATCTATTGTAATCGTCCGATAGAAGAAATAC
TTTCTCATGATAAGCTGTCAGTTCTGTGATGAAGATTCAGACTGGGAGGATGTACTGTAA
TCCTGGCGAGGAGAATGAGAATTT
>OriGene 3' read for NM_002095 unedited
NNNTTTTTCCTATGGACCGCGGGCCGCATTCTAGGATCGAGTTTTTTTTTTTTTTTTTTT
TTTTTTTCCTTCTACAGGGATTTTATTATAAATATGTTCTTTTGGAATAAACAAATATTG
CATTCAAGACTATGGGCAAACTTGCTCTAAAGTAACAAACGAGGAAGAAAGGAACCAGGA
ACCATTTAAAAGTGGAGAAAAACACAGCAAAGACACCTGCATGCCACGCTCAGCGCCTTT
CTCCCAGGGAGTAACAGAAGGTTAACAGGGAACATCACATTGCCCATGAGCCCATTCTAC
TCAAATAAGCTTTGAGTTTGGTAATTTGCACTTGTTTTGTAAACTGAACTGCTCCTCTCC
TCAGCCGCAAGAAGCAGATAGGAAGACAGTCTTCAGACCCCGAGCATCAGCAAGAACACT
CTTGATTGGGTATCTGTAACTCTGTTCCAGGGCAAAACTGTTCCCTATTTGCTGGAAGTA
ATGTCAGAGTAATCCTTCAGCACTCCAGCCAAGTGTTCGTTATGAGTCTTAAAGCGTCGC
TTTTTCTGTGAAGCAGGCTTTTTCCTTCTCTGAATAGGGGCCACTTTCTTTGGTCCAGAT
TCCTGCATGGAAGAAATACCCTGTCGCTTCAGATATTCTTCAATTTTCTCCTCGTCCATG
GAATCTACAGTGACACTCCTCCACAGTTTCTGAAATTCTTCATCCACAGAAAACTGACAG
CTCTTATCATTGAAGAAAAGTATTTTCTTCTTATCGGGACGATTTACAAATAGTATCTGG
TCCCCCCAAGCCTTGACAGCTTTCTGGGAATTGGGCAGTGCTTCTTCTATTGTCTCTAAA
GAATTCTCCTAACCTCGCTGGCATGCTGATCTAAGAGCCTAGTAGGGCCTTCTTATCTCT
CACGTGTACTTGGGCTG
Restriction Sites NotI-NotI     
ACCN NM_002095
Insert Size 1640 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_002095.3, NP_002086.1
RefSeq Size 1560 bp
RefSeq ORF 1560 bp
Locus ID 2961
UniProt ID P29084
Domains TFIIE_beta
Protein Families Transcription Factors
Protein Pathways Basal transcription factors
Gene Summary The general transcription factor IIE (TFIIE) is part of the RNA polymerase II transcription initiation complex, recruiting TFIIH and being essential for promoter clearance by RNA polymerase II. TFIIE is a heterodimer (and sometimes heterotetramer) of alpha and beta subunits. The protein encoded by this gene represents the beta subunit of TFIIE. [provided by RefSeq, Jan 2017]
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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