TFIIE beta (GTF2E2) (NM_002095) Human Untagged Clone
CAT#: SC118828
GTF2E2 (untagged)-Human general transcription factor IIE, polypeptide 2, beta 34kDa (GTF2E2)
CNY 3,872.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | FE; TF2E2; TFIIE-B; TTD6 |
Vector | pCMV6-XL4 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>NCBI ORF sequence for NM_002095, the custom clone sequence may differ by one or more nucleotides
ATGGATCCAAGCCTGTTGAGAGAAAGGGAGCTGTTCAAAAAACGAGCTCTTTCTACTCCTGTAGTAGAAA AACGTTCAGCATCTTCTGAGTCATCATCATCATCGTCAAAGAAGAAGAAAACAAAGGTAGAACATGGAGG ATCGTCAGGCTCTAAACAAAATTCTGATCATAGCAATGGATCATTTAACTTGAAAGCTTTGTCAGGAAGC TCTGGATATAAGTTTGGTGTTCTTGCTAAGATTGTGAATTACATGAAGACACGGCATCAGCGAGGAGATA CGCATCCTCTAACCTTAGATGAAATTTTGGATGAAACACAACATTTAGATATTGGACTCAAGCAGAAACA ATGGCTAATGACTGAGGCTTTAGTCAACAATCCCAAAATTGAAGTAATAGATGGGAAGTATGCTTTCAAG CCCAAGTACAACGTGAGAGATAAGAAGGCCCTACTTAGGCTCTTAGATCAGCATGACCAGCGAGGATTAG GAGGAATTCTTTTAGAAGACATAGAAGAAGCACTGCCCAATTCCCAGAAAGCTGTCAAGGCTTTGGGGGA CCAGATACTATTTGTAAATCGTCCCGATAAGAAGAAAATACTTTTCTTCAATGATAAGAGCTGTCAGTTT TCTGTGGATGAAGAATTTCAGAAACTGTGGAGGAGTGTCACTGTAGATTCCATGGACGAGGAGAAAATTG AAGAATATCTGAAGCGACAGGGTATTTCTTCCATGCAGGAATCTGGACCAAAGAAAGTGGCCCCTATTCA GAGAAGGAAAAAGCCTGCTTCACAGAAAAAGCGACGCTTTAAGACTCATAACGAACACTTGGCTGGAGTG CTGAAGGATTACTCTGACATTACTTCCAGCAAATAG >OriGene 5' read for NM_002095 unedited
TATATTTGTATACGACTTACTATAGGGCGGCCGCGAATTCGGCACGAGGCTAGCACCCGA TCGGGAAGTGGCGGGCGGAGTCCCGGGTCCAGTCGCCGCCTCAGCTACCGCCGCTGCCGC CGCCGCCGCCGCCACCGCCAGTGGTGAGACCCCGACCTGGCGGGTCAGCGCTGGGCGTGC GTGCGGGCAGGCGGGGGCGCTGACGAGAAGCAGGAAGAGGGTGCAGTGCCGGCGTGGGCG GCCGGCCGAGGCGGAGGCGCAGGAAGGGGGCGGCGAGTCGTGCGAGGCTGCCCTTCTCAC TCAGCATTATGGATCCAAGCCTGTTGAGAGAAAGGGAGCTGTTCAAAAAACGAGCTCTTT CTACTCCTGTAGTAGAAAAACGTTCAGCATCTTCTGAGTCATCATCATCATCGTCAAAGA AGAAGAAAACAAAGGTAGAACATGGAGGATCGTCAGGCTCTAAACAAAATTCTGATCATA GCAATGGATCATTTAACTTGAAAGCTTTGTCAGGAAGCTCTGGATATAAGTTTGGTGTTC TTGCTAAGATTGTGAATTACATGAAGACACGGCATCAGCGAGGAGATACGCATCCTCTAA CCTTAGATGAAATTTTGGATGAAACACAACATTTAGATATTGGACTCAAGCAGAAACAAT GGCTAATGACTGAGGCTTTAGTCAACAATCCCAAAATTGAAGTAATAGATGGGAAGTATG CTTTCAAGCCCAAGTACAACGTGAGAGATAAGAAGGCCCTACTTANGCTCTTAGATCAGC ATGACCAGCGAGGATTAGGGAGGATTCTTTTAGAAGACATAGAAGAAGCACTGCCCAATT CCCAGAAGCTGTCAGGGCTTTGGGGACAGATCTATTGTAATCGTCCGATAGAAGAAATAC TTTCTCATGATAAGCTGTCAGTTCTGTGATGAAGATTCAGACTGGGAGGATGTACTGTAA TCCTGGCGAGGAGAATGAGAATTT >OriGene 3' read for NM_002095 unedited
NNNTTTTTCCTATGGACCGCGGGCCGCATTCTAGGATCGAGTTTTTTTTTTTTTTTTTTT TTTTTTTCCTTCTACAGGGATTTTATTATAAATATGTTCTTTTGGAATAAACAAATATTG CATTCAAGACTATGGGCAAACTTGCTCTAAAGTAACAAACGAGGAAGAAAGGAACCAGGA ACCATTTAAAAGTGGAGAAAAACACAGCAAAGACACCTGCATGCCACGCTCAGCGCCTTT CTCCCAGGGAGTAACAGAAGGTTAACAGGGAACATCACATTGCCCATGAGCCCATTCTAC TCAAATAAGCTTTGAGTTTGGTAATTTGCACTTGTTTTGTAAACTGAACTGCTCCTCTCC TCAGCCGCAAGAAGCAGATAGGAAGACAGTCTTCAGACCCCGAGCATCAGCAAGAACACT CTTGATTGGGTATCTGTAACTCTGTTCCAGGGCAAAACTGTTCCCTATTTGCTGGAAGTA ATGTCAGAGTAATCCTTCAGCACTCCAGCCAAGTGTTCGTTATGAGTCTTAAAGCGTCGC TTTTTCTGTGAAGCAGGCTTTTTCCTTCTCTGAATAGGGGCCACTTTCTTTGGTCCAGAT TCCTGCATGGAAGAAATACCCTGTCGCTTCAGATATTCTTCAATTTTCTCCTCGTCCATG GAATCTACAGTGACACTCCTCCACAGTTTCTGAAATTCTTCATCCACAGAAAACTGACAG CTCTTATCATTGAAGAAAAGTATTTTCTTCTTATCGGGACGATTTACAAATAGTATCTGG TCCCCCCAAGCCTTGACAGCTTTCTGGGAATTGGGCAGTGCTTCTTCTATTGTCTCTAAA GAATTCTCCTAACCTCGCTGGCATGCTGATCTAAGAGCCTAGTAGGGCCTTCTTATCTCT CACGTGTACTTGGGCTG |
Restriction Sites | NotI-NotI |
ACCN | NM_002095 |
Insert Size | 1640 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_002095.3, NP_002086.1 |
RefSeq Size | 1560 bp |
RefSeq ORF | 1560 bp |
Locus ID | 2961 |
UniProt ID | P29084 |
Domains | TFIIE_beta |
Protein Families | Transcription Factors |
Protein Pathways | Basal transcription factors |
Gene Summary | The general transcription factor IIE (TFIIE) is part of the RNA polymerase II transcription initiation complex, recruiting TFIIH and being essential for promoter clearance by RNA polymerase II. TFIIE is a heterodimer (and sometimes heterotetramer) of alpha and beta subunits. The protein encoded by this gene represents the beta subunit of TFIIE. [provided by RefSeq, Jan 2017] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC203477 | GTF2E2 (Myc-DDK-tagged)-Human general transcription factor IIE, polypeptide 2, beta 34kDa (GTF2E2) |
CNY 2,400.00 |
|
RC203477L3 | Lenti ORF clone of Human general transcription factor IIE, polypeptide 2, beta 34kDa (GTF2E2), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC203477L4 | Lenti ORF clone of Human general transcription factor IIE, polypeptide 2, beta 34kDa (GTF2E2), mGFP tagged |
CNY 5,890.00 |
|
RG203477 | GTF2E2 (tGFP-tagged) - Human general transcription factor IIE, polypeptide 2, beta 34kDa (GTF2E2) |
CNY 4,000.00 |
|
SC320510 | GTF2E2 (untagged)-Human general transcription factor IIE, polypeptide 2, beta 34kDa (GTF2E2) |
CNY 2,400.00 |
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