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Glutathione Peroxidase 3 (GPX3) (NM_002084) Human Untagged Clone
CAT＃: SC118819
GPX3 (untagged)-Human glutathione peroxidase 3 (plasma) (GPX3) (10ug), (Note: selenocysteine protein, Internal stop codon present. see reference data summary below)
CNY 3,432.00
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Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Synonyms | GPx-P; GSHPx-3; GSHPx-P |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC118819 sequence for NM_002084 edited (data generated by NextGen Sequencing)
ATGGCCCGGCTGCTGCAGGCGTCCTGCCTGCTTTCCCTGCTCCTGGCCGGCTTCGTCTCG CAGAGCCGGGGACAAGAGAAGTCGAAGATGGACTGCCATGGTGGCATAAGTGGCACCATT TACGAGTACGGAGCCCTCACCATTGATGGGGAGGAGTACATCCCCTTCAAGCAGTATGCT GGCAAATACGTCCTCTTTGTCAACGTGGCCAGCTACTGAGGCCTGACGGGCCAGTACATT GAACTGAATGCACTACAGGAAGAGCTTGCACCATTCGGTCTGGTCATTCTGGGCTTTCCC TGCAACCAATTTGGAAAACAGGAACCAGGAGAGAACTCAGAGATCCTTCCTACCCTCAAG TATGTCCGACCAGGTGGAGGCTTTGTCCCTAATTTCCAGCTCTTTGAGAAAGGGGATGTC AATGGAGAGAAAGAGCAGAAATTCTACACTTTCCTAAAGAACTCCTGTCCTCCCACCTCG GAGCTCCTGGGTACATCTGACCGCCTCTTCTGGGAACCCATGAAGGTTCACGACATCCGC TGGAACTTTGAGAAGTTCCTGGTGGGGCCAGATGGTATACCCATCATGCGCTGGCACCAC CGGACCACGGTCAGCAACGTCAAGATGGACATCCTGTCCTACATGAGGCGGCAGGCAGCC CTGGGGGTCAAGAGGAAGTAA Clone variation with respect to NM_002084.3 >OriGene 5' read for NM_002084 unedited
TGCGTCGAGATTTGTAAACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCCTCGTG CCCGAATTCGCACGAGGGGGATCAGGCAGCGGCTCAGGCGACCCTGAGTGTGCCCCCACC CCGCCATGGCCCGGCTGCTGCAGGCGTCCTGCCTGCTTTCCCTGCTCCTGGCCGGCTTCG TCTCGCAGAGCCGGGGACAAGAGAAGTCGAAGATGGACTGCCATGGTGGCATAAGTGGCA CCATTTACGAGTACGGAGCCCTCACCATTGATGGGGAGGAGTACATCCCCTTCAAGCAGT ATGCTGGCAAATACGTCCTCTTTGTCAACGTGGCCAGCTACTGAGGCCTGACGGGCCAGT ACATTGAACTGAATGCACTACAGGAAGAGCTTGCACCATTCGGTCTGGTCATTCTGGGCT TTCCCTGCAACCAATTTGGAAAACAGGAACCAGGAGAGAACTCAGAGATCCTTCCTACCC TCAAGTATGTCCGACCAGGTGGAGGCTTTGTCCCTAATTTCCAGCTCTTTGAGAAAGGGG ATGTCAATGGAGAGAAAGAGCAGAAATTCTACACTTTCCTAAAGAACTCCTGTCCTCCCA CCTCGGAGCTCCCTGGTACATCTGACCGCCTCTTCTGGGAACCCATGAAGGTCACGACAT CCGCTGGAACTTTGAGAAGTTCCTGGTGGGGCCAGATGGTATACCCATCATGCGCTGGCA CCCACGGACCACGGTCAGCAACGTCAAGATGGACATCCCTGTCTACATGAGGCGGCCAGC AGCCCTGGGGGTCAAAGAGAAGTAAACTGAGGCCGTCTCATCCCATGTCCCACATGTAGG GGAGGAACTTTTGTTCAGGAAGAAATCCGTGTCTTCCACACACTATCTACCCATCACAGA CCCCTTTCCTATCACTCAGG >OriGene 3' read for NM_002084 unedited
NNNTTTTAGCTATGGACCGGCGGCCGCAATCTAGNGTCGAGTTTTTTTTTTTTTTTTTTT TTTTGCTGCAGAAAGGCTTTTACTGGGCAGACGGGGTGAGTCTCAAGCCAGTGGACCAGT GAGGGGTGAGGGCACGTCCTCCGAAGGAGCAGGGGTGGCATCCCTGCCCAGGGGCCTTAG CCTGAATGCACTAAGGGCTGGCCCCTCAGACAGGCTCAGGGGAGGTCCGCCCACAAGGGC TTTGGGCCCCTCCCTCATGAAGACGCCACCCCTGCCATGGGGTCCGTGGCCCTTGCTGTC ACATCTGCCTTGGTTGATCTCAGGGAGGTTGATCTCTCTCAGGGAGAACTGTGGGGTGGG GATGTAGCCTTGGGAGGGCCCTTCAGGAAGTAAGAGTGGGGGGTTGGGGAGTGTTNGTAN ACCCANAAACTTCTGGGGACGTCAGTCATAGTTACTAATATTTGGAGGCAGTGGGAGATG CTGGCCCCAAGGTTGAGGTATCAGTTAGAGCAGAACAATTGGACCTAGAGCTGGTTTTTC CTTTGGGTTTAGGTGTAAGTGAACTATTATCATTGGAGTGGAGAACTGGAGAGAAAGGGT TGTCACTGTCCAGCACTACACAGCTGCAGGCACACAGATGGTACACCATTCCAGAAAGAC ACATAGGCTGACATGTACTTGGNCACNNATGCNNACACACNCAATNANGCATACCTGGAG GCATGNGGGNAAACACCACATGCACCCACACCCCATGCCTGGCAGTACAAGAACTGTTGC ATTCATTTGTGCCAGGCTGGGCCCTGACAGAAGGAAAGGGGCTGTGTTGTTACACCCCCT GTCTGCAAACCCTCCTTTCTTAAAAAATCCTCCCCTCTGGGGCATCGCAGAACGGCTTAT TCCTTCCTCCAACCCCG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_002084 |
| Insert Size | 1660 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). The expression of this clone is not guaranteed due to the nature of selenoproteins. |
| OTI Annotation | This clone encodes a selenoprotein containing the rare amino acid selenocysteine (Sec). Sec is encoded by UGA codon, which normally signals translational termination. Expression of this clone is not guaranteed due to the nature of selenoproteins. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002084.2, NP_002075.2 |
| RefSeq Size | 1856 bp |
| Locus ID | 2878 |
| UniProt ID | P22352 |
| Protein Families | Druggable Genome, Secreted Protein |
| Protein Pathways | Arachidonic acid metabolism, Glutathione metabolism |
| Gene Summary | The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of organic hydroperoxides and hydrogen peroxide (H2O2) by glutathione, and thereby protect cells against oxidative damage. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme is secreted, and is abundantly found in plasma. Downregulation of expression of this gene by promoter hypermethylation has been observed in a wide spectrum of human malignancies, including thyroid cancer, hepatocellular carcinoma and chronic myeloid leukemia. This isozyme is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2016] Transcript Variant: This variant (1) represents the predominant transcript and encodes isoform 1. |
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207758 | GPX3 (Myc-DDK-tagged)-Human glutathione peroxidase 3 (plasma) (GPX3), (Note, selenocysteine protein, internal stop codon, see reference data summary) |
CNY 2,400.00 |
|
| RC207758L1 | Lenti-ORF, GPX3 (Myc-DDK-tagged)-Human glutathione peroxidase 3 (plasma) (GPX3), (Note, selenocystein protein, internal stop codon, see summary) |
CNY 4,800.00 |
|
| RG207758 | GPX3 (GFP-tagged) - Human glutathione peroxidase 3 (plasma) (GPX3), (10ug,) (Note: selenocysteine protein, Internal stop codon present. see reference data summary below) |
CNY 4,000.00 |
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