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GIRK1 (KCNJ3) (NM_002239) Human Untagged Clone
CAT＃: SC118769
KCNJ3 (untagged)-Human potassium inwardly-rectifying channel, subfamily J, member 3 (KCNJ3)
CNY 5,608.00
| Cited in 3 publications. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | GIRK1; KGA; KIR3.1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC118769 sequence for NM_002239 edited (data generated by NextGen Sequencing)
ATGTCTGCACTCCGAAGGAAATTTGGGGACGATTATCAGGTAGTGACCACATCGTCCAGC GGCTCGGGCTTGCAGCCCCAGGGGCCAGGCCAGGACCCTCAGCAGCAGCTTGTGCCCAAG AAGAAGCGGCAGCGGTTCGTGGACAAGAACGGCCGGTGCAATGTACAGCACGGCAACCTG GGCAGCGAGACAAGCCGCTACCTCTCGGACCTCTTCACCACGCTGGTGGACCTCAAGTGG CGCTGGAACCTCTTCATCTTCATTCTCACCTACACCGTGGCCTGGCTTTTCATGGCGTCC ATGTGGTGGGTGATCGCCTACACTCGGGGCGACCTGAACAAAGCCCACGTCGGTAACTAC ACGCCTTGCGTGGCCAATGTCTATAACTTCCCTTCTGCCTTCCTCTTCTTCATCGAGACG GAGGCCACCATCGGCTATGGCTACCGATACATCACAGACAAGTGCCCCGAGGGCATCATC CTCTTCCTCTTCCAGTCCATCCTGGGCTCCATCGTGGACGCCTTCCTCATCGGCTGCATG TTCATCAAGATGTCCCAGCCCAAGAAGCGCGCCGAGACCCTCATGTTCAGCGAGCACGCG GTGATCTCCATGAGGGACGGAAAACTCACGCTTATGTTCCGGGTGGGCAACCTGCGCAAC AGCCACATGGTCTCCGCGCAGATTCGCTGCAAGCTGCTCAAATCTCGGCAGACACCTGAG GGTGAGTTCCTTCCCCTTGACCAACTTGAACTGGATGTAGGTTTTAGTACAGGGGCAGAT CAACTTTTTCTTGTGTCCCCCCTCACAATTTGCCACGTGATCGATGCCAAAAGCCCCTTT TATGACCTATCCCAGCGAAGCATGCAAACTGAACAGTTCGAGATTGTCGTCATCCTAGAA GGCATTGTGGAAACAACTGGGATGACTTGTCAAGCTCGAACATCATATACTGAAGATGAA GTTCTTTGGGGTCATCGTTTTTTTCCTGTAATTTCCTTAGAAGAGGGATTCTTTAAAGTT GATTACTCCCAGTTCCATGCAACATTTGAAGTCCCCACCCCACCTTACAGTGTGAAAGAG CAGGAGGAAATGCTTCTCATGTCGTCCCCTTTAATAGCACCAGCCATAACTAACAGCAAA GAAAGACATAATTCTGTGGAATGCTTAGATGGACTAGATGATATTACTACAAAACTACCA TCTAAGCTGCAGAAAATTACTGGAAGAGAAGACTTTCCCAAAAAACTCTTGAGGATGAGT TCTACAACTTCAGAAAAAGCCTACAGCTTGGGAGACTTGCCCATGAAACTTCAACGAATA AGTTCAGTTCCGGGCAACTCAGAAGAAAAACTGGTATCTAAAACCACCAAGATGTTATCT GATCCCATGAGCCAGTCTGTGGCTGATTTGCCACCAAAGCTTCAAAAGATGGCTGGAGGA GCAGCTAGGATGGAAGGGAACCTTCCAGCCAAATTAAGAAAAATGAACTCTGATCGCTTC ACATAA Clone variation with respect to NM_002239.2 >OriGene 5' read for NM_002239 unedited
GCATTTGTATACGACTCCTATAGGGCGGCCGCGATTCGGCACGAGGGTGACCCGGGATGC CGGTGGTGGGGAAAGAAAAGAGAGCCTCTGGAAGCTTGGAGGCAAAATTGCGCTTGGGTT CCTGTTCCTTGCATCCCTCCTGGCTTGAGTGCGGGAGAACACTTTTTAAAGACTCACCTT GGAAAGAAGGCCTCCGTCCCAGGGGAGAAGGAGAGGCGTCTGCAGGGGGCAGAGACCGCA GCTACCTGCCGGGTGCGCCCCCCACCCAGGAGCGCTCGCTTCGCCCCCTTTCCTCCCCCG CCCCCACCTCCTTATTGGTGCTAGTTTGCAGCGCCCAGCTCCTGCGCCTTCGCTTCGCGT TTGAATCTGGCTCGCCCCTTCGTATTATGTCTGCACTCCGAAGGAAATTTGGGGACGATT ATCAGGTAGTGACCACATCGTCCAGCGGCTCGGGCTTGCAGCCCCAGGGGCCAGGCCAGG ACCCTCAGCAGCAGCTTGTGCCCAAGAAGAAGCGGCAGCGGTTCGTGGACAAGAACGGCC GGTGCAATGTACAGCACGGCAACCTGGGCAGCGAGACAAGCCGCTACCTCTCGGACCTCT TCACCACGCTGGTGGACCTCAAGTGGCGCTGGAACCTCTTCATCTTCATTCTCACCTACA CCGTGGCCTGGCTTTTCATGGCGTCCATGTGGTGGGGTGATCGCCTACACTCGGNGCGAC CTGAACAAGCCCACGTCGGTAACTACACGCCTTGCGTGGCCAATGTCTATAACTTCCTTN CTGCCTTCCTCTTCTTCATCGAGACGGAGGCCACCATCGGCTATGGCTACCGATACATCA CAGACAAGTGCCCCCGAGGCATCATNCTCTTCCTCTTNCAGNCCATNCTGGGCTCCATCG GGGGN >OriGene 3' read for NM_002239 unedited
CTCCCCACCCCCCCCACCCACCTTTTTCCCTCCTTACACACAATTTGACTTTGNACCGCG GNCCGCATACTAGNGATCGGTTTTTTTTTTTTTTTTTTGAAATGTTTCCAATTTACTCAG TTTGGAACAGGGACAATTAATTGTGTAACTTCACACAGATTCTGATATATTCAATATAAC TTGGCTACTGTCTATTAACATGATTCAGTGTTTGTTCAGAGCTCATCTTTGTATTAGATC ATATACAAAACAGATTTCCAGATTTTAATTAGTCTAGGAAAGGAATAATTCTCAAATTAA TCAACAATTATTTCTCCCCAGTACTAGCAACTTTTAAAAATCATAAGAAATGTTCAATGC TATACTGAGATCTGGTATGCTCTAAAATTTACAATTTAAACATGTAATGGTTATATTTTT GCAGGGCCATAGATAGCACAAACTTGTGTGATTCTCTCATATAAAATACAATTGAAAGCC TCCCAAGACCTTAAACAGCAGAAAACATTAAATCACATTTTAAAATGACAAAAATTTAAG GCTAAAATATTTGGTATTAATAATTAATGCCAGTAGCCTTCCTACTCTAATAAAGTAAAC ATGCACAAGAACTACAACTTTGAACAAACCTCACAATTTTTATCAGATATATATGCATGT ATGTATGACTGCGGATGTATGTGCATATATATATGCAAAAGGCCGGGCCACCTTACCCCC CCCCCCATCCCCCCTCCTTTTCATCCCCCTCGTCCTGCCACATTACTCCCCTCTGTCCCG GGGTTACTACCATTTCGTCAGAACGCTCCCCCTTGACGCTCCTATCTTTCACACTCTTAC AATTCCATTTTCATATTCCCTAACTTTCCCTGTCCCCTTTCTCTCCTCCCCGCCCCCCTG TCCTCATTCTCAAAGGCGTCACCTTCCCCCTTCATCCTCCTCCTCTACGTCCCACCCCTC GCCACCATCCTCAAG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_002239 |
| Insert Size | 2870 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_002239.2, NP_002230.1 |
| RefSeq Size | 2890 bp |
| RefSeq ORF | 1506 bp |
| Locus ID | 3760 |
| UniProt ID | P48549 |
| Domains | IRK |
| Protein Families | Druggable Genome, Ion Channels: Potassium, Transmembrane |
| Gene Summary | Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. The protein encoded by this gene is an integral membrane protein and inward-rectifier type potassium channel. The encoded protein, which has a greater tendency to allow potassium to flow into a cell rather than out of a cell, is controlled by G-proteins and plays an important role in regulating heartbeat. It associates with three other G-protein-activated potassium channels to form a heteromultimeric pore-forming complex that also couples to neurotransmitter receptors in the brain and whereby channel activation can inhibit action potential firing by hyperpolarizing the plasma membrane. These multimeric G-protein-gated inwardly-rectifying potassium (GIRK) channels may play a role in the pathophysiology of epilepsy, addiction, Down's syndrome, ataxia, and Parkinson's disease. Alternative splicing results in multiple transcript variants encoding distinct proteins. [provided by RefSeq, May 2012] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1; also known as GIRK1a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Citations (3)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Pathophysiological and Pharmacological Characteristics of KCNJ5 157-159delITE Somatic Mutation in Aldosterone-Producing Adenomas
,null,
Biomedicines
,PubMed ID 34440230
[KCNJ3]
|
|
A Novel KCNJ5-insT149 Somatic Mutation Close to, but Outside, the Selectivity Filter Causes Resistant Hypertension by Loss of Selectivity for Potassium
,Kuppusamy, M;Caroccia, B;Stindl, J;Bandulik, S;Lenzini, L;Gioco, F;Fishman, V;Zanotti, G;Gomez-Sanchez, C;Bader, M;Warth, R;Rossi, GP;,
J. Clin. Endocrinol. Metab. July 2014
,PubMed ID 25057880
[KCNJ3]
|
|
A Novel KCNJ5-insT149 Somatic Mutation Close to,but Outside, the Selectivity Filter Causes ResistantHypertension by Loss of Selectivity for Potassium
,Kuppusamy, M;Caroccia, B;Stindl, J;Bandulik, S;,
J Clin Endocrinol Metab
[KCNJ3]
|
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205322 | KCNJ3 (Myc-DDK-tagged)-Human potassium inwardly-rectifying channel, subfamily J, member 3 (KCNJ3) |
CNY 3,728.00 |
|
| RC205322L1 | Lenti ORF clone of Human potassium inwardly-rectifying channel, subfamily J, member 3 (KCNJ3), Myc-DDK-tagged |
CNY 6,128.00 |
|
| RC205322L2 | Lenti ORF clone of Human potassium inwardly-rectifying channel, subfamily J, member 3 (KCNJ3), mGFP tagged |
CNY 6,460.00 |
|
| RC205322L3 | Lenti ORF clone of Human potassium inwardly-rectifying channel, subfamily J, member 3 (KCNJ3), Myc-DDK-tagged |
CNY 6,128.00 |
|
| RC205322L4 | Lenti ORF clone of Human potassium inwardly-rectifying channel, subfamily J, member 3 (KCNJ3), mGFP tagged |
CNY 6,460.00 |
|
| RG205322 | KCNJ3 (tGFP-tagged) - Human potassium inwardly-rectifying channel, subfamily J, member 3 (KCNJ3) |
CNY 5,320.00 |
