P2RX7 (AB209709) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	P2X7
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AB209709, the custom clone sequence may differ by one or more nucleotides CTGTGGCCCTGTCAGGAAGAGTAGAGCTCTGGTCCAGCTCCGCGCAGGGAGGGAGGCTGTCACCATGCCG GCCTGCTGCAGCTGCAGTGATGTTTTCCAGTATGAGACGAACAAAGTCACTCGGATCCAGAGCATGAATT ATGGCACCATTAAGTGGTTCTTCCACGTGATCATCTTTTCCTACGTTTGCTTTGCTCTGGTGAGTGACAA GCTGTACCAGCGGAAAGAGCCTGTCATCAGTTCTGTGCACACCAAGGTGAAGGGGATAGCAGAGGTGAAA GAGGAGATCGTGGAGAATGGAGTGAAGAAGTTGGTGCACAGTGTCTTTGACACCGCAGACTACACCTTCC CTTTGCAGGGGAACTCTTTCTTCGTGATGACAAACTTTCTCAAAACAGAAGGCCAAGAGCAGCGGTTGTG TCCCGAGTATCCCACCCGCAGGACGCTCTGTTCCTCTGACCGAGGTTGTAAAAAGGGATGGATGGACCCG CAGAGCAAAGGAATTCAGACCGGAAGGTGTGTAGTGCATGAAGGGAACCAGAAGACCTGTGAAGTCTCTG CCTGGTGCCCCATCGAGGCAGTGGAAGAGGCCCCCCGGCCTGCTCTCTTGAACAGTGCCGAAAACTTCAC TGTGCTCATCAAGAACAATATCGACTTCCCCGGCCACAACTACACCACGAGAAACATCCTGCCAGGTTTA AACATCACTTGTACCTTCCACAAGACTCAGAATCCACAGTGTCCCATTTTCCGACTAGGAGACATCTTCC GAGAAACAGGCGATAATTTTTCAGATGTGGCAATTCAGGGCGGAATAATGGGCATTGAGATCTACTGGGA CTGCAACCTAGACCGTTGGTTCCATCACTGCCGTCCCAAATACAGTTTCCGTCGCCTTGACGACAAGACC ACCAACGTGTCCTTGTACCCTGGCTACAACTTCAGATACGCCAAGTACTACAAGGAAAACAATGTTGAGA AACGGACTCTGATAAAAGTCTTCGGGATCCGTTTTGACATCCTGGTTTTTGGCACCGGAGGAAAATTTGA CATTATCCAGCTGGTTGTGTACATCGGCTCAACCCTCTCCTACTTCGGTCTGGTAAGAGATTCTCTTTTC CATGCTTTAGGAAAATGGTTTGGAGAAGGAAGTGACTAACGCAGCGCTTGTCTGCATTCTCCCCAGGCCA CTGTGTTCATCGACTTCCTCATCGACACTTACTCCAGTAACTGCTGTCGCTCCCATATTTATCCCTGGTG CAAGTGCTGTCAGCCCTGTGTGGTCAACGAATACTACTACAGGAAGAAGTGCGAGTCCATTGTGGAGCCA AAGCCGACATTAAAGTATGTGTCCTTTGTGGATGAATCCCACATTAGGATGGTGAACCAGCAGCTACTAG GGAGAAGTCTGCAAGATGTCAAGGGCCAAGAAGTCCCAAGACCTGCGATGGACTTCACAGATTTGTCCAG GCTGCCCCTGGCCCTCCATGACACACCCCCGATTCCTGGACAACCAGAGGAGATACAGCTGCTTAGAAAG GAGGCGACTCCTAGATCCAGGGATAGCCCCGTCTGGTGCCAGTGTGGAAGCTGCCTCCCATCTCAACTCC CTGAGAGCCACAGGTGCCTGGAGGAGCTGTGCTGCCGGAAAAAGCCGGGGGCCTGCATCACCACCTCAGA GCTGTTCAGGAAGCTGGTCCTGTCCAGACACGTCCTGCAGTTCCTCCTGCTCTACCAGGAGCCCTTGCTG GCGCTGGATGTGGATTCCACCAACAGCCGGCTGCGGCACTGTGCCTACAGGTGCTACGCCACCTGGCGCT TCGGCTCCCAGGACATGGCTGACTTTGCCATCCTGCCCAGCTGCTGCCGCTGGAGGATCCGGAAAGAGTT TCCAAAGAGTGAAGGGCAGTACAGTGGCTTCAAGAGTCCTTACTGAAGCCAGGCACCGTGGCTCACGTCT GTAATCCCAGCGCTTTGGGAGGCCGAGGCAGGCAGATCACCTGAGGTCGGGAGTTGGAGACCCGCCTGGC TAACAAGGCGAAATCCTGTCTGTACTAAAAATACAAAAATCAGCCAGACATGGTGGCATGCACCTGCAAT CCCAGCTACTCGGGAGGCTGAGGCACAAGAATCACTTGAACCCGGGAGGCAGAGGTTGTAGTGAGCCCAG ATTGTGCCACTGCTCTCCAGCCTGGGAGGCACAGCAAACTGTCCC 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AB209709 unedited NGGTCAAAATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGTGGCCCTG TCAGGAAGAGTAGAGCTCTGGTCCAGCTCCGCGCAGGGAGGGAGGCTGTCACCATGCCGG CCTGCTGCAGCTGCAGTGATGTTTTCCAGTATGAGACGAACAAAGTCACTCGGATCCAGA GCATGAATTATGGCACCATTAAGTGGTTCTTCCACGTGATCATCTTTTCCTACGTTTGCT TTGCTCTGGTGAGTGACAAGCTGTACCAGCGGAAAGAGCCTGTCATCAGTTCTGTGCACA CCAAGGTGAAGGGGATAGCAGAGGTGAAAGAGGAGATCGTGGAGAATGGAGTGAAGAAGT TGGTGCACAGTGTCTTTGACACCGCAGACTACACCTTCCCTTTGCAGGGGAACTCTTTCT TCGTGATGACAAACTTTCTCAAAACAGAAGGCCAAGAGCAGCGGTTGTGTCCCGAGTATC CCACCCGCAGGACGCTCTGTTCCTCTGACCGAGGTTGTAAAAAGGGATGGATGGACCCGC AGAGCAAAGGAATTCAGACCGGAAGGTGTGTAGTGCATGAAGGGAACCAGAAGACCTGTG AAGTCTCTGCCTGGTGCCCCATCGAGGCAGTGGAAGAGGCCCCCCGGCCTGCTCTCTTGA ACAGTGCCGAAACTTTCACTGTGCTCATCAAGAACAATATCGACTTCCCCGGGCCACACT ACACCACGAGAAACATCCTGCCAGGTTTAAACATCACTTGTACCTTCCACAAGACTCAGA ATCCACAGTGTCCCATTTTTCGACTANGAGACATCTTCCGAGAAACAGGCGATAATTTTT CAGATGTGGCAATCAGGNNCGGATATGGGCCATTGAGATCTACTGGGACTGCACCTANAC CG 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AB209709 unedited AGGTCGTTGCTGTGCCCCCCCAGTCTGGNATACAGTGGCACAATCTGGGCTCACTACAAC CTCTGCCTCCCGGGTTCAAGTGATTCTTGTGCCTCAGCCTCCCGAGTAGCTGGGATTGCA GGTGCATGCCACCATGTCTGGCTGATTTTTGTATTTTTAGTACAGACAGGATTTCGCCTT GTTAGCCAGGCGGGTCTCCAACTCCCGACCTCAGGTGATCTGCCTGCCTCGGCCTCCCAA AGCGCTGGGATTACAGACGTGAGCCACGGTGCCTGGCTTCAGTAAGGACTCTTGAAGCCA CTGTACTGCCCTTCACTCTTTGGAAACTCTTTCCGGATCCTCCAGCGGCAGCAGCTGGGC AGGATGGCAAAGTCAGCCATGTCCTGGGAGCCGAAGCGCCAGGTGGCGTAGCACCTGTAG GCACAGTGCCGCAGCCGGCTGTTGGTGGAATCCACATCCAGCGCCAGCAAGGGCTCCTGG TAGAGCAGGAGGAACTGCAGGACGTGTCTGGACAGGACCAGCTTCCTGAACAGCTCTGAG GTGGTGATGCAGGCCCCCGGCTTTTTCCGGCAGCACAGCTCCTCCAGGCACCTGTGGCTC TCANGGAGTTGAGATGGGAGGCAGCTTCCACACTGGCACCAGACGGGGCTATCCCTGGAT CTAGGAGTCGCCTCCTTTCTAAGCAGCTGTATCTCCTCTGGTTGTCCAGGAATCGGGGGT GTGTCATGGAAGGCCAGGGGCAGCCTGGACAAATCTGTGAAAGTCATCACAAGTCCTTGG GACTTCTTGGCCCTTTGACATCTTTGCAGAACTTCCTCCCTGATAGG
Restriction Sites	NotI-NotI
ACCN	AB209709
Insert Size	2240 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AB209709.1, BAD92946.1
RefSeq Size	2215 bp
RefSeq ORF	2215 bp
Locus ID	5027
Protein Families	Druggable Genome, Ion Channels: ATP Receptors, Transmembrane
Protein Pathways	Calcium signaling pathway, Neuroactive ligand-receptor interaction
Gene Summary	The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel and is responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Activation of this nuclear receptor by ATP in the cytoplasm may be a mechanism by which cellular activity can be coupled to changes in gene expression. Multiple alternatively spliced variants have been identified, most of which fit nonsense-mediated decay (NMD) criteria. [provided by RefSeq, Jul 2010]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.