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OCLN (NM_002538) Human Untagged Clone
CAT＃: SC118584
OCLN (untagged)-Human occludin (OCLN), transcript variant 1
CNY 5,840.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | BLCPMG; PPP1R115; PTORCH1 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC118584 sequence for NM_002538 edited (data generated by NextGen Sequencing)
ATGTCATCCAGGCCTCTTGAAAGTCCACCTCCTTACAGGCCTGATGAATTCAAACCGAAT CATTATGCACCAAGCAATGACATATATGGTGGAGAGATGCATGTTCGACCAATGCTCTCT CAGCCAGCCTACTCTTTTTACCCAGAAGATGAAATTCTTCACTTCTACAAATGGACCTCT CCTCCAGGAGTGATTCGGATCCTGTCTATGCTCATTATTGTGATGTGCATTGCCATCTTT GCCTGTGTGGCCTCCACGCTTGCCTGGGACAGAGGCTATGGAACTTCCCTTTTAGGAGGT AGTGTAGGCTACCCTTATGGAGGAAGTGGCTTTGGTAGCTACGGAAGTGGCTATGGCTAT GGCTATGGTTATGGCTATGGCTACGGAGGCTATACAGACCCAAGAGCAGCAAAGGGCTTC ATGTTGGCCATGGCTGCCTTTTGTTTCATTGCCGCGTTGGTGATCTTTGTTACCAGTGTT ATAAGATCTGAAATGTCCAGAACAAGAAGATACTACTTAAGTGTGATAATAGTGAGTGCT ATCCTGGGCATCATGGTGTTTATTGCCACAATTGTCTATATAATGGGAGTGAACCCAACT GCTCAGTCTTCTGGATCTCTATATGGTTCACAAATATATGCCCTCTGCAACCAATTTTAT ACACCTGCAGCTACTGGACTCTACGTGGATCAGTATTTGTATCACTACTGTGTTGTGGAT CCCCAGGAGGCCATTGCCATTGTACTGGGGTTCATGATTATTGTGGCTTTTGCTTTAATA ATTTTCTTTGCTGTGAAAACTCGAAGAAAGATGGACAGGTATGACAAGTCCAATATTTTG TGGGACAAGGAACACATTTATGATGAGCAGCCCCCCAATGTCGAGGAGTGGGTTAAAAAT GTGTCTGCAGGCACACAGGACGTGCCTTCACCCCCATCTGACTATGTGGAAAGAGTTGAC AGTCCCATGGCATACTCTTCCAATGGCAAAGTGAATGACAAGCGGTTTTATCCAGAGTCT TCCTATAAATCCACGCCGGTTCCTGAAGTGGTTCAGGAGCTTCCATTAACTTCGCCTGTG GATGACTTCAGGCAGCCTCGTTACAGCAGCGGTGGTAACTTTGAGACACCTTCAAAAAGA GCACCTGCAAAGGGAAGAGCAGGAAGGTCAAAGAGAACAGAGCAAGATCACTATGAGACA GACTACACAACTGGCGGCGAGTCCTGTGATGAGCTGGAGGAGGACTGGATCAGGGAATAT CCACCTATCACTTCAGATCAACAAAGACAACTGTACAAGAGGAATTTTGACACTGGCCTA CAGGAATACAAGAGCTTACAATCAGAACTTGATGAGATCAATAAAGAACTCTCCCGTTTG GATAAAGAATTGGATGACTATAGAGAAGAAAGTGAAGAGTACATGGCTGCTGCTGATGAA TACAATAGACTGAAGCAAGTGAAGGGATCTGCAGATTACAAAAGTAAGAAGAATCATTGC AAGCAGTTAAAGAGCAAATTGTCACACATCAAGAAGATGGTTGGAGACTATGATAGACAG AAAACATAG Clone variation with respect to NM_002538.3 >OriGene 5' read for NM_002538 unedited
NNGGGGCCNNNNNNNNNNNNNAACCCCNNGGTTCAGAATTGTATACGACTCACTATAGGC GGCCGCGAATTCGGCACCAGAGGAAACCGAGAACCAGGTCCAGAGCGCCGAGGAGCCGGT CTAGGACGCAGCAGATTGGTTTATCTTGGAAGCTAAAGGGCATTGCTCATCCTGAAGATC AGCTGACCATTGACAATCAGCCATGTCATCCAGGCCTCTTGAAAGTCCACCTCCTTACAG GCCTGATGAATTCAAACCGAATCATTATGCACCAAGCAATGACATATATGGTGGAGAGAT GCATGTTCGACCAATGCTCTCTCAGCCAGCCTACTCTTTTTACCCAGAAGATGAAATTCT TCACTTCTACAAATGGACCTCTCCTCCAGGAGTGATTCGGATCCTGTCTATGCTCATTAT TGTGATGTGCATTGCCATCTTTGCCTGTGTGGCCTCCACGCTTGCCTGGGACAGAGGCTA TGGAACTTCCCTTTTAGGAGGTAGTGTAGGCTACCCTTATGGAGGAAGTGGCTTTGGTAG CTACGGAAGTGGCTATGGCTATGGCTATGGTTATGGCTATGGCTACGGAGGCTATACAGA CCCAAGAGCAGCAAAGGGCTTCATGTTGGCCATGGCTGCCTTTTGTTTCATTGCCGCGTT GGTGATCTTTGTTACCAGTGTTATAAGATCTGANATGTCCAGAACAAGAAGATACTACTT AAGTGTGATAATAGTGAGTGCTATCCTGGGCATCATGGTGTTNNATTGCCACANTGTCTA TATAATGGGAGTGAACCCNACTGCTCAGTCTTCTGGATCTCTATATGGNTCACAAATATA TGCCCTCTGNCACCAATTTATACACCTGCAGCTACTGGACTCTACGTGGATCAGTATTGT ATCACTACTGNNGTNGGGNATCCCCAGNAGGCCATNGNNCATNGTACTGGGGNNTCATGA TTATGNGGGCTNNTGCTTATAAATNTCTTTGCTGN >OriGene 3' read for NM_002538 unedited
GTTGAGTTTTATTTTTTTTTTTTTTTACTTTTACATGATCTTTATTATTTAAGAAAAACC TCTTTTAACCATTTATATAACAGAAAAAAAATAGGGAGGCTGGTAGATCATCACATATAT AGTAGCTAAAATATGAAAGGCCAGGGAATTTATTATTAATGAAGTCATAAAACAGACTTA ACCAAAAGTGTGTGCTAGGAAACAAGCAGTTTCACTTCAGAGACTTCATTGCAGGAACCC AGTTTCCTTATGTGGAAAAAAGTGATTATAAATAACAGTTATCTGAAAGGTGGTTGAGAG GATTAAATGAGATCACCTATGCAAACAAATACATGTAGGTATGAAGACATCGTCTGGGGT GTGAAAGTTTAGTTCACACCAGAACCTTCCTTTAAGGCTTAAAAACCTCAAACAGTATTT CATTCTTAATACAAGAACCAATGTTTAAAGCATAAAATCCTTAATTGGAGTGTTCAGCCC AGTTGATTATCAAAAGCGATTTATAAAATGCTTCAATACTGATGATGTTAAAGCAACCAA AACTTTGTGATGCTCACAGAGGTTTGGCTTCCGGGGTTATGGTCCAAAGTCATTTGCCTT CTGAGAAGATTGCAGAGATGTCAGATACTTAATTTCTCAAACAACTTGGCATCAGCCTTC TATGTTTTCTGTCTATCATAGTCTCCCACCATCTTCTTGATGTGTGACAATTTGCTCTTT AACTGGCTGCAATGANNTCTNNCTACTTTGNAATCTGCAGATCCCCTCACTTGCTTCAGT CTATTGTATTCATCAGCAGCAGCCATGTACTCTTCACTTTCTTCTCTATAGTCATCCAAT TTCTTTATCCAACGGA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_002538 |
| Insert Size | 2500 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002538.2, NP_002529.1 |
| RefSeq Size | 2648 bp |
| RefSeq ORF | 1569 bp |
| Locus ID | 100506658 |
| UniProt ID | Q16625 |
| Domains | MARVEL |
| Gene Summary | This gene encodes an integral membrane protein that is required for cytokine-induced regulation of the tight junction paracellular permeability barrier. Mutations in this gene are thought to be a cause of band-like calcification with simplified gyration and polymicrogyria (BLC-PMG), an autosomal recessive neurologic disorder that is also known as pseudo-TORCH syndrome. Alternative splicing results in multiple transcript variants. A related pseudogene is present 1.5 Mb downstream on the q arm of chromosome 5. [provided by RefSeq, Apr 2011] Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). Both variants 1 and 2 encode isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206468 | OCLN (Myc-DDK-tagged)-Human occludin (OCLN), transcript variant 1 |
CNY 5,832.00 |
|
| RC206468L1 | Lenti ORF clone of Human occludin (OCLN), transcript variant 1, Myc-DDK-tagged |
CNY 8,232.00 |
|
| RC206468L2 | Lenti ORF clone of Human occludin (OCLN), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC206468L3 | Lenti ORF clone of Human occludin (OCLN), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC206468L4 | Lenti ORF clone of Human occludin (OCLN), transcript variant 1, mGFP tagged |
CNY 8,232.00 |
|
| RG206468 | OCLN (tGFP-tagged) - Human occludin (OCLN), transcript variant 1 |
CNY 7,432.00 |
|
| SC322559 | OCLN (untagged)-Human occludin (OCLN), transcript variant 1 |
CNY 5,840.00 |
