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POLA2 (NM_002689) Human Untagged Clone
CAT＃: SC118517
POLA2 (untagged)-Human polymerase (DNA directed), alpha 2 (70kD subunit) (POLA2)
CNY 4,464.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC118517 sequence for NM_002689 edited (data generated by NextGen Sequencing)
ATGTCCGCATCCGCCCAGCAGCTGGCGGAGGAGCTGCAGATCTTCGGCCTAGACTGCGAG GAGGCTCTAATTGAGAAATTGGTAGAGCTTTGTGTTCAGTATGGACAGAATGAGGAGGGA ATGGTAGGCGAGCTTATAGCCTTCTGCACCAGCACACATAAAGTTGGCCTTACCTCAGAG ATCCTGAACTCTTTTGAGCATGAGTTTCTGAGCAAAAGATTATCGAAAGCCAGGCATAGT ACCTGCAAGGACAGTGGCCATGCAGGAGCTAGAGACATTGTTTCCATTCAAGAGCTAATT GAAGTGGAAGAAGAAGAGGAAATCCTCTTGAACTCTTACACCACACCTTCAAAGGGTTCT CAGAAGCGAGCTATCTCTACCCCAGAAACCCCCCTAACAAAAAGGAGTGTGTCAACTCGT AGCCCCCATCAGCTACTCTCACCGTCAAGTTTCTCTCCAAGTGCTACTCCCTCCCAGAAA TACAACTCACGAAGTAACCGAGGAGAAGTGGTTACCTCCTTCGGCTTAGCACAGGGAGTA TCTTGGTCTGGGAGAGGAGGAGCTGGAAACATCAGCCTGAAGGTCTTGGGATGTCCAGAG GCACTAACTGGGAGCTACAAATCCATGTTTCAGAAGCTCCCAGACATTCGAGAAGTTCTG ACCTGTAAGATAGAAGAACTTGGCAGCGAACTCAAGGAACATTACAAGATTGAAGCTTTC ACTCCTTTGCTAGCCCCAGCACAGGAGCCTGTCACTCTGCTGGGCCAGATTGGCTGTGAT AGCAACGGGAAGCTGAACAACAAGTCAGTGATTCTCGAGGGAGACCGGGAACATTCCTCG GGTGCTCAAATTCCAGTGGATTTATCTGAGCTTAAGGAATATTCTCTGTTTCCTGGACAG GTTGTAATTATGGAAGGAATCAACACCACTGGTAGGAAACTTGTTGCCACCAAACTCTAC GAGGGTGTGCCACTTCCATTTTATCAGCCCACTGAAGAGGATGCAGACTTTGAGCAAAGC ATGGTCCTGGTTGCCTGTGGACCATACACCACATCTGACAGCATCACGTATGACCCCCTG CTTGACCTGATTGCTGTCATCAACCATGACCGGCCAGATGTCTGCATCCTGTTTGGCCCT TTCCTGGATGCTAAGCATGAACAGGTGGAGAATTGTCTACTGACAAGTCCATTTGAAGAC ATTTTCAAGCAGTGTCTACGAACAATTATTGAAGGCACAAGAAGCTCCGGCTCCCACCTT GTCTTTGTCCCGTCATTGAGAGATGTGCACCATGAGCCTGTGTACCCCCAGCCGCCTTTC AGCTACTCCGATCTGTCTCGAGAGGACAAAAAGCAAGTACAGTTTGTGTCCGAGCCCTGC AGCCTCTCCATAAACGGAGTGATCTTCGGCTTGACATCCACAGATCTGCTTTTCCACCTG GGGGCCGAGGAGATCAGTAGTTCTTCCGGAACTTCAGACAGATTCAGCCGAATACTCAAG CACATCTTGACCCAGAGGAGCTACTACCCACTCTACCCGCCCCAAGAAGACATGGCCATT GACTATGAGTCGTTCTATGTTTACGCACAGCTGCCTGTCACCCCAGATGTCCTCATCATC CCGTCAGAGCTGAGGTACTTCGTGAAGGATGTCCTCGGCTGTGTCTGTGTGAACCCTGGG CGCCTTACCAAAGGGCAGGTGGGAGGCACCTTCGCCCGACTCTACCTTAGGAGGCCGGCA GCGGACGGGGCAGAGAGGCAGAGCCCATGCATTGCTGTGCAGGTCGTCAGGATCTGA Clone variation with respect to NM_002689.2 >OriGene 5' read for NM_002689 unedited
TGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGGCCACTCAGTTCTG CCACCGTCACTGAGAAGCTCAGCGGTAGCTTTTGGGAAGCAGGACGTTCTCACCAGGAGA GCGTCCTCTCGAGATTTCTGCTCCCTCCATTCAGGGCGTTTGGGAGCCACCCCTTCATTT TTTAAAAAAAGTATTTCTCTGTGACCGACGGCCGGGGCCTTCTGACGGTCTGAGGTCTTG CTTGGGCCAGTCACCTCCTGTCACGGTCCGCGGAGGGGGGAAGGATAAGAGGGCGAGGAG CTCATCGCTCGCCACCCCCGTGGGCTTCTTGGGCGCAGGTCGGAGCTGGGTGGGCCGGCT CCCCGGCCCCTGGCTTGGGCGACCATGTCCGCATCCGCCCAGCAGCTGGCGGAGGAGCTG CAGATCTTCGGCCTAGACTGCGAGGAGGCTCTAATTGAGAAATTGGTAGAGCTTTGTGTT CAGTATGGACAGAATGAGGAGGGAATGGTAGGCGAGCTTATAGCCTTCTGCACCAGCACA CATAAAGTTGGCCTTACCTCAGAGATCCTGAACTCTTTTGAGCATGAGTTTCTGAGCAAA AGATTATCGAAAGCCAGGCATAGTACCTGCAAGGACAGTGGCCATGCAGGAGCTAGAGAC ATTGTTTCCATTCAAGAGCTAATTGAAGTGGAAGAAGAAGAGGAAATCCTCTTGAACTCT TACACCACACCTTCAAAGGGTTCTCAGAAGCGAGCTATCTCTACCCCAGAAACCCCCCTA CAAAAGGAGTGTGTCAACTCGTAGCCCCATCAGCTACTCTCACCGCAAGTTTCTCTCCAG TGCTACTCCCTCCAGAATACACTCACGAGTACCCGAGAGAAGTGGTTACTNCTTTCGCTA GC >OriGene 3' read for NM_002689 unedited
GCCGCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTACACACTCAGAATTTAA TTTCTCCAAAAGTTGTTTCTGTGTTTGATTCATGTAAAAAATCACAAAGCCTTCTCGCCT TTTCTGAGTGGGCGGGATCCACAGCTGGCTTACTTCTGGACACGGAGCATGGACTGAGAA GCAAGAGCTTCCTTCCAGGAGAGGCACCACTCAGAGGCAGGCCCGGCTGCACAAGTCCTC CCTGCTGGGCCTGGTCACTGCAGCTGCCCCTCCCTGGCTGGCTCCTCTCTCCTTTCCCAC CCAACTGTTCACCTTGTCACAGGGCTATGTCTTGGCTCTTGGCTAAGACTTTAAGGGCCC ACACAGCAGAGAACAGCAGAGGACAGAAGCCTCAGATCCTGACGACCTGCACAGCAATGC ATGGGCTCTGCCTCTCTGCCCCGTCCGCTGCCGGCCTCCTAAGGTAAAGTCGGGCGAAGG TGCCTCCCACCTGCCCTTTGGTAAGGCGCCCAGGGTTCACACAGACACAGCCGAGGACAT CCTTCACGAAGTACCTCACCTCTGACGGGATGATGAGGACATCTGGGGTGACAGGCAGCT GTGCGTAAACCTAGAACCGACTATAGTCAATGGCCATGTCTTCTTGGGGCCCGACACAGT GGGTAGTAACCTACCTTGGGACAAGAAGCGCCTTGAGTATTCCGGCTGAAATCTGCCCCG AAGTTCCTGAAAAACCTACTGGATTCACCTCNGGCCCCCACGGGGAAAAAGCAAATCTGG GGGCCGTNAAGCCCAAAAAACACTCCCGCTTTATGGGAAAGGCTTGACGGTCTCCGACCC ACACCGTGATCTGGGTTTTTGGGCCCCCCGATACAATACCGTGAACCCTCAAAGGGGCTC GGGGGGTCCCAGCGCCCCATGTGCCCACTTCTTAATGAACGGAACAAGACAAGGGGGGG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_002689 |
| Insert Size | 2560 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002689.2, NP_002680.2 |
| RefSeq Size | 2514 bp |
| RefSeq ORF | 1797 bp |
| Locus ID | 23649 |
| UniProt ID | Q14181 |
| Domains | DNA_pol_alpha_B |
| Protein Pathways | DNA replication, Metabolic pathways, Purine metabolism, Pyrimidine metabolism |
| Gene Summary | May play an essential role at the early stage of chromosomal DNA replication by coupling the polymerase alpha/primase complex to the cellular replication machinery.[UniProtKB/Swiss-Prot Function] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201145 | POLA2 (Myc-DDK-tagged)-Human polymerase (DNA directed), alpha 2 (70kD subunit) (POLA2) |
CNY 4,456.00 |
|
| RC201145L3 | Lenti ORF clone of Human polymerase (DNA directed), alpha 2 (70kD subunit) (POLA2), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201145L4 | Lenti ORF clone of Human polymerase (DNA directed), alpha 2 (70kD subunit) (POLA2), mGFP tagged |
CNY 5,890.00 |
|
| RG201145 | POLA2 (tGFP-tagged) - Human polymerase (DNA directed), alpha 2 (70kD subunit) (POLA2) |
CNY 4,370.00 |
