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PI4KB (NM_002651) Human Untagged Clone
CAT＃: SC118488
PI4KB (untagged)-Human phosphatidylinositol 4-kinase, catalytic, beta (PI4KB), transcript variant 1
CNY 15,870.00
| Cited in 2 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | NPIK; PI4K-BETA; PI4K92; PI4KBETA; PI4KIII; PI4KIIIBETA; PIK4CB |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_002651, the custom clone sequence may differ by one or more nucleotides
ATGAGATTCTTGGAAGCTCGAAGTCTGGCTGTGGCCATGGGAGATACAGTAGTGGAGCCTGCCCCCTTGA AGCCAACTTCTGAGCCCACTTCTGGCCCACCAGGGAATAATGGGGGGTCCCTGCTAAGTGTCATCACGGA GGGGGTCGGGGAACTATCAGTGATTGACCCTGAGGTGGCCCAGAAGGCCTGCCAGGAGGTGTTGGAGAAA GTCAAGCTTTTGCATGGAGGCGTGGCAGTCTCTAGCAGAGGCACCCCACTGGAGTTGGTCAATGGGGATG GTGTGGACAGTGAGATCCGTTGCCTAGATGATCCACCTGCCCAGATCAGGGAGGAGGAAGATGAGATGGG GGCCGCTGTGGCCTCAGGCACAGCCAAAGGAGCAAGAAGACGGCGGCAGAACAACTCAGCTAAACAGTCT TGGCTGCTGAGGCTGTTTGAGTCAAAACTGTTTGACATCTCCATGGCCATTTCATACCTGTATAACTCCA AGGAGCCTGGAGTACAAGCCTACATTGGCAACCGGCTCTTCTGCTTTCGCAACGAGGACGTGGACTTCTA TCTGCCCCAGTTGCTTAACATGTACATCCACATGGATGAGGACGTGGGTGATGCCATTAAGCCCTACATA GTCCACCGTTGCCGCCAGAGCATTAACTTTTCCCTCCAGTGTGCCCTGTTGCTTGGGGCCTATTCTTCAG ACATGCACATTTCCACTCAACGACACTCCCGTGGGACCAAGCTACGGAAGCTGATCCTCTCAGATGAGCT AAAGCCAGCTCACAGGAAGAGGGAGCTGCCCTCCTTGAGCCCGGCCCCTGACACAGGGCTGTCTCCCTCC AAAAGGACTCACCAGCGCTCTAAGTCAGATGCCACTGCCAGCATAAGTCTCAGCAGCAACCTGAAACGAA CAGCCAGCAACCCTAAAGTGGAGAATGAGGATGAGGAGCTCTCCTCCAGCACCGAGAGTATTGATAATTC ATTCAGTTCCCCTGTTCGACTGGCTCCTGAGAGAGAATTCATCAAGTCCCTGATGGCGATCGGCAAGCGG CTGGCCACGCTCCCCACCAAAGAGCAGAAAACACAGAGGCTGATCTCAGAGCTCTCCCTGCTCAACCATA AGCTCCCTGCCCGAGTCTGGCTGCCCACTGCTGGCTTTGACCACCACGTGGTCCGTGTACCCCACACACA GGCTGTTGTCCTCAACTCCAAGGACAAGGCTCCCTACCTGATTTATGTGGAAGTCCTTGAATGTGAAAAC TTTGACACCACCAGTGTCCCTGCCCGGATCCCCGAGAACCGAATTCGGAGTACGAGGTCCGTAGAAAACT TGCCCGAATGTGGTATTACCCATGAGCAGCGAGCTGGCAGCTTCAGCACTGTGCCCAACTATGACAACGA TGATGAGGCCTGGTCGGTGGATGACATAGGCGAGCTGCAAGTGGAGCTCCCCGAAGTGCATACCAACAGC TGTGACAACATCTCCCAGTTCTCTGTGGACAGCATCACCAGCCAGGAGAGCAAGGAGCCTGTGTTCATTG CAGCAGGGGACATCCGCCGGCGCCTTTCGGAACAGCTGGCTCATACCCCGACAGCCTTCAAACGAGACCC AGAAGATCCTTCTGCAGTTGCTCTCAAAGAGCCCTGGCAGGAGAAAGTACGGCGGATCAGAGAGGGCTCC CCCTACGGCCATCTCCCCAATTGGCGGCTCCTGTCAGTCATTGTCAAGTGTGGGGATGACCTTCGGCAAG AGCTTCTGGCCTTTCAGGTGTTGAAGCAACTGCAGTCCATTTGGGAACAGGAGCGAGTGCCCCTTTGGAT CAAGCCATACAAGATTCTTGTGATTTCGGCTGATAGTGGCATGATTGAACCAGTGGTCAATGCTGTGTCC ATCCATCAGGTGAAGAAACAGTCACAGCTCTCCTTGCTCGATTACTTCCTACAGGAGCACGGCAGTTACA CCACTGAGGCATTCCTCAGTGCACAGCGCAATTTTGTGCAAAGTTGTGCTGGGTACTGCTTGGTCTGCTA CCTGCTGCAAGTCAAGGACAGACACAATGGGAATATCCTTTTGGACGCAGAAGGCCACATCATCCACATC GACTTTGGCTTCATCCTCTCCAGCTCACCCCGAAATCTGGGCTTTGAGACGTCAGCCTTTAAGCTGACCA CAGAGTTTGTGGATGTGATGGGCGGCCTGGATGGCGACATGTTCAACTACTATAAGATGCTGATGCTGCA AGGGCTGATTGCCGCTCGGAAACACATGGACAAGGTGGTGCAGATCGTGGAGATCATGCAGCAAGGTTCT CAGCTTCCTTGCTTCCATGGCTCCAGCACCATTCGAAACCTCAAAGAGAGGTTCCACATGAGCATGACTG AGGAGCAGCTGCAGCTGCTGGTGGAGCAGATGGTGGATGGCAGTATGCGGTCTATCACCACCAAACTCTA TGACGGCTTCCAGTACCTCACCAACGGCATCATGTGA >OriGene 5' read for NM_002651 unedited
NGGGGGGTCAAATTTGTACACGACTCATATAGGGCGGCCGCGATTCGGCACGAGGATTTT ATTTTATTTTATTATTTTTTTGGTCTTGGTTGTACTGAGGGAGGAAGAAGAGGTTGTGTG GCCCGGTCGAACTTGTGGCAGCCTGAAGGCCCCCTCAGGCGGCGCCGCGGGCAGCCCCGC AGCCGGGGCCTGGTGCAGCCTCCGCGGCCGCTGTCAGGGAAGCGCAGGCGGCCAATGGAA CCCGGGAGCGGTCGCTGCTGCTGAGGCGGCAGTGTCGGCAGTCCAACCGCGACTGCCCGC ACCCCCTCCGCGGGGGTCCCCCAGAGGATCAACTAAACCTTGAACTAAGAAGAAAAATGT GTTGTGAGCAGGGGGAGCCTCAGCTGCCTCAGGCCGTTCAGGACAGAAGGGTGTTTCTGA AGGCCGGAGCAAGTTTTGAAGAAGTCCCTATCAGATTACACTTGGTTGACTACTCCGGAG CAGCCACTAAGAGGGATGAACAGGCCTGCGTGGAAATTGAATGAGATTCTTGGAAGCTCG AAGTCTGGCTGTGGCCATGGGAGATACAGTAGTGGAGCCTGCCCCCTTGAAGCCAACTTC TGAGCCCACTTCTGGCCCACCAGGGAATAATGGGGGGTCCCTGCTAAGTGTCATCACGGA GGGGGTCGGNGAACTATCAGTGATTGACCCTTGAGTGGCCCAGAAGCCTGCCAGGAGGTG TTGGAGAAAGTCAAGCTTTTGCATGGAGGCGTGGCAGTCTCTAGCAGAGGCACCCACTGG GAGTTGTCATGGGGGATGGTGTGGACCGTGANTATCCGTGCCTANATGATCCACCTGCCC AATCAGGGAGGAGGAATAGATTAGGCGGCCCGTTTTGCCCTAGGCCACTCCC >OriGene 3' read for NM_002651 unedited
TAGAGGCATTTTTTCAACAAAATTAAAAATAAAAAATTGANAGCTCTTTNNTCCCTGGGT TTGGGNGAAGGGGAGTCAGGGTAGGGGAATTCCACATGGCTAGGCCAGTACCCTCAGTAC ACTGGAGGGCTGTGGGAATTCCCTTCCTTGCCCATGATGGGGAACCTCAGCAAGTGCAGG GCCCACGCAGCGCCCGCCAGCAAGGGGAGCTTGGGCCAGGGGTCGGTCCCTGGGAGGGGT AGGTCCCTGGGAGGGGTAGAGCTGGGCAGCAGCATGGCCAGTGGCCAACCACAGGATCAA GTCCTAAGTACCGAGAACCTACTCTTCCCCACTTCCTGGGCCCCAGAACGGAATAAGAGG ATGGCCTGTGGAAAGAACCTCTGAGAGACCCCTACAAGGAGAAGAAAGGCCCCAGTGTCC CTCACATTTGTCACCTCTGTTTTCTGGAGGGCAGCGAGTCCTGGAGTCAGTCTGGTGGTA ATACTGACACAGACCAGGAGGGGCATGGAAGCCCCAACAAGTCTGGACCCCACAGCTGGC TCTCCCACCCCTTAGCAAGCTCTCGCAGTTACCACATGATCCTTCGTGCCTCTTGCCCTC CATTTCCCTTGGGCGGATGGCTGGCGCAGGGGGGCCTCCTGGCTTCGGCGCTCCCAGAAA AGGCCCTCTTAGGAAGGTGCCCTGCACCCCCCCCATTCTGGGACTGCCACTGCGTCCACT TATCCTCCTCGTGACCCCTCCACCCCCCCACCCTTCGCGTTTCACACCCCTCCTCCCTTC CCTCTTTCTCCCCCTTACTCTCCCTCCCCCTCAACCTCCCATGATCCCACATCTCCGTTC CAAACCGTCTGCTTCTCCTCTCCCCACCCATAATCTTCTCAAAAAACTGCCTACCTCCCT CTCCCCCTTCTCTCTCCCATCCACCCCCCCACCNTTACCTTCTTTCCCCCCTCTCCCCCC TTT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_002651 |
| Insert Size | 3780 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002651.1, NP_002642.1 |
| RefSeq Size | 3471 bp |
| RefSeq ORF | 2487 bp |
| Locus ID | 5298 |
| UniProt ID | Q9UBF8 |
| Domains | PI3_PI4_kinase |
| Protein Families | Druggable Genome |
| Protein Pathways | Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system |
| Gene Summary | Phosphorylates phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate (PIP). May regulate Golgi disintegration/reorganization during mitosis, possibly via its phosphorylation. Involved in Golgi-to-plasma membrane trafficking (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Class III Phosphatidylinositol 4-Kinase Alpha and Beta Are Novel Host Factor Regulators of Hepatitis C Virus Replication
,null,
,PubMed ID 19605471
[PI4KB]
|
|
Class III phosphatidylinositol-4 kinase alpha & beta are novel host factor regulators of hepatitis C virus replication
,Jason Borawski, Philip Troke, Xiaoling Puyang, Veronica Gibaja, ShanChaun Zhao, Craig Mickanin, Juliet Leighton-Davies, Christopher J. Wilson, Vic Myer, Ivan CornellaTaracido, Jeremy Baryza, John Tallarico, Gerard Joberty, Marcus Bantscheff, Markus Schirle, Tewis Bouwmeester, Joanna E. Mathy, Kai Lin, Teresa Compton, Mark Labow, Brigitte Wiedmann, and L. Alex Gaither,
J. Virol., Jul 2009; 10.1128/JVI.02418-08.
[PI4KB]
|
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216570 | PI4KB (Myc-DDK-tagged)-Human phosphatidylinositol 4-kinase, catalytic, beta (PI4KB), transcript variant 1 |
CNY 9,096.00 |
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| RC216570L1 | Lenti-ORF clone of PI4KB (Myc-DDK-tagged)-Human phosphatidylinositol 4-kinase, catalytic, beta (PI4KB), transcript variant 1 |
CNY 11,496.00 |
|
| RC216570L2 | Lenti-ORF clone of PI4KB (mGFP-tagged)-Human phosphatidylinositol 4-kinase, catalytic, beta (PI4KB), transcript variant 1 |
CNY 10,550.00 |
|
| RC216570L3 | Lenti-ORF clone of PI4KB (Myc-DDK-tagged)-Human phosphatidylinositol 4-kinase, catalytic, beta (PI4KB), transcript variant 1 |
CNY 10,550.00 |
|
| RC216570L4 | Lenti-ORF clone of PI4KB (mGFP-tagged)-Human phosphatidylinositol 4-kinase, catalytic, beta (PI4KB), transcript variant 1 |
CNY 10,550.00 |
|
| RG216570 | PI4KB (tGFP-tagged) - Human phosphatidylinositol 4-kinase, catalytic, beta (PI4KB), transcript variant 1 |
CNY 10,696.00 |
