PGM1 (NM_002633) Human Untagged Clone
CAT#: SC118479
PGM1 (untagged)-Human phosphoglucomutase 1 (PGM1), transcript variant 1
CNY 4,192.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | CDG1T; GSD14 |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC118479 sequence for NM_002633 edited (data generated by NextGen Sequencing)
ATGGTGAAGATCGTGACAGTTAAGACCCAGGCGTACCAGGACCAGAAGCCGGGCACGAGC GGGCTGCGGAAGCGGGTGAAGGTGTTCCAGAGCAGCGCCAACTACGCGGAGAACTTCATC CAGAGTATCATCTCCACCGTGGAGCCGGCGCAGCGGCAGGAGGCCACGCTGGTGGTGGGC GGGGACGGCCGGTTCTACATGAAGGAGGCCATCCAGCTCATCGCTCGCATCGCTGCCGCC AACGGGATCGGTCGCTTGGTTATCGGACAGAATGGAATCCTCTCCACCCCTGCTGTATCC TGCATCATTAGAAAAATCAAAGCCATTGGTGGGATCATTCTGACAGCCAGTCACAACCCA GGGGGCCCCAATGGAGATTTTGGAATCAAATTCAATATTTCTAATGGAGGTCCTGCTCCA GAAGCAATAACTGATAAAATTTTCCAAATCAGCAAGACAATTGAAGAATATGCAGTTTGC CCTGACCTGAAAGTAGACCTTGGTGTTCTGGGAAAGCAGCAGTTTGACTTGGAAAATAAG TTCAAACCCTTCACAGTGGAAATTGTGGATTCGGTAGAAGCTTATGCTACAATGCTGAGA AGCATCTTTGATTTCAGTGCACTGAAAGAACTACTTTCTGGGCCAAACCGACTGAAGATC CGTATTGATGCTATGCATGGAGTTGTGGGACCGTATGTAAAGAAGATCCTCTGTGAAGAA CTCGGTGCCCCTGCGAACTCGGCAGTTAACTGCGTTCCTCTGGAGGACTTTGGAGGCCAC CACCCTGACCCCAACCTCACCTATGCAGCTGACCTGGTGGAGACCATGAAGTCAGGAGAG CATGATTTTGGGGCTGCCTTTGATGGAGATGGGGATCGAAACATGATTCTGGGCAAGCAT GGGTTCTTTGTGAACCCTTCAGACTCTGTGGCTGTCATTGCTGCCAACATCTTCAGCATT CCGTATTTCCAGCAGACTGGGGTCCGCGGCTTTGCACGGAGCATGCCCACGAGTGGTGCT CTGGACCGGGTGGCTAGTGCTACAAAGATTGCTTTGTATGAGACCCCAACTGGCTGGAAG TTTTTTGGGAATTTGATGGACGCGAGCAAACTGTCCCTTTGTGGGGAGGAGAGCTTCGGG ACCGGTTCTGACCACATCCGTGAGAAAGATGGACTGTGGGCTGTCCTTGCCTGGCTCTCC ATCCTAGCCACCCGCAAGCAGAGTGTGGAGGACATTCTCAAAGATCATTGGCAAAAGCAT GGCCGGAATTTCTTCACCAGGTATGATTACGAGGAGGTGGAAGCTGAGGGCGCAAACAAA ATGATGAAGGACTTGGAGGCCCTGATGTTTGATCGCTCCTTTGTGGGGAAGCAGTTCTCA GCAAATGACAAAGTTTACACTGTGGAGAAGGCCGATAACTTTGAATACAGCGACCCAGTG GATGGAAGCATTTCAAGAAATCAGGGCTTGCGCCTCATTTTCACAGATGGTTCTCGAATC GTCTTCCGACTGAGCGGCACTGGGAGTGCCGGGGCCACCATTCGGCTGTACATCGATAGC TATGAGAAGGACGTTGCCAAGATTAACCAGGACCCCCAGGTCATGTTGGCCCCCCTTATT TCCATTGCTCTGAAAGTGTCCCAGCTGCAGGAGAGGACGGGACGCACTGCACCCACTGTC ATCACCTAA Clone variation with respect to NM_002633.2 1258 t=>c >OriGene 5' read for NM_002633 unedited
GGGCAGTATTTGTATACGACTCATATAGGGCGGCCGCGATTCGGCACGAGGCCGCCAGCC AAGTCCGCCGCTCTGACCCCCGGCAGCAAGTCGCCACCATGGTGAAGATCGTGACAGTTA AGACCCAGGCGTACCAGGACCAGAAGCCGGGCACGAGCGGGCTGCGGAAGCGGGTGAAGG TGTTCCAGAGCAGCGCCAACTACGCGGAGAACTTCATCCAGAGTATCATCTCCACCGTGG AGCCGGCGCAGCGGCAGGAGGCCACGCTGGTGGTGGGCGGGGACGGCCGGTTCTACATGA AGGAGGCCATCCAGCTCATCGCTCGCATCGCTGCCGCCAACGGGATCGGTCGCTTGGTTA TCGGACAGAATGGAATCCTCTCCACCCCTGCTGTATCCTGCATCATTAGAAAAATCAAAG CCATTGGTGGGATCATTCTGACAGCCAGTCACAACCCAGGGGGCCCCAATGGAGATTTTG GAATCAAATTCAATATTTCTAATGGAGGTCCTGCTCCAGAAGCAATAACTGATAAAATTT TCCAAATCAGCAAGACAATTGAAGAATATGCAGTTTGCCCTGACCTGAAAGTAGACCTTG GTGTTCTGGGAAAGCAGCAGTTTGACTTGGAAATAAGTTCAACCCTTCACAGTGGAAATT GTGGATTCGGTAGAAGCTTATGCTACAATGCTGAGAAGCATCTTTGATTTCAGTGCACCT GAAGAACTACCTTTCTGGNCCAAACCGACTGAAGATCCGTATTGATGCTATGCATGAAGT TGTTGGGACCGTATGTAAAGAAGATCCTCTGTGAAGGACTTCGTGCCCCTGCGGACTCCG GAGTTAACTTGCGTCCTCTGGNAGACTTTGAAAGGCACCACCCTGACCCCACCTTACCTA GCAGCTGACCTGGGGG >OriGene 3' read for NM_002633 unedited
TAGCTCTGGACCGGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTGTAGCT TATTTTCCTTTATTATCCCAAAAATCTGTGTGAATGGGACAATGTCGCAAAACAAGGCAG AAATCTGTAGCCAAGTACGTCTCACTCAGAGGAAAGAACTGATTGGACAGAAGGCACTAG AAATTGCATTTTGTTGGGTTACATGTAAACAGGAAATGGGGGGAAAGATTCACAAAAGAG AAAGGAACTTGCACCATCATTTCATTGCCACATCCTAAGGATGAAGAATGGCCTCACTCA CTGCTGATGCTCCACCTATAGCTTACATTGTAGTGTAAACTGTACCTGATGGCTAAGGAG ACAAATACCCACGCAGCAGCAATGCAGGAGGGCATGAAAAGGAATTGAGATTGATCTAAG CCCGCAGGTCCTCTTTCCCTCACAGCTCCCAAGGGCATCTTGTTTGGCAGTGCCTTGTAA ATGCACTGCTCGTCAACTGGTTAGTGAAAAAGTCAAATCCTAAAATGCTTGGTGAATACA TTTTGTTTCTGTCATGCTCTTCAATCAGATGACTTGGATGGGTCCGGGGGTGGAGGGACG TACCACATCAGGCCTGTCTTCTTAGGTGATGACAGTGGGTGCAGTGCGTCCCGTCCTCTC CTGCAGCTGGGACACTTTCAGAGCAATGGAAATAAGGGGGGCCAACATGACCTGGGGGTC CTGGTTAATCTTGGCAACGTCCTTCTCATAGCTATCGATGTACAGCCGAATGGTGGCCCC CGCACTCCCAGTGCCGCTCAGTCGGAAGACGATTCGAGAACCATCTGTGAAAATGAGCGC AGCCCTGATTTCTTGAATGCTTNCTCCACTGGGTCGCTGNATTCAAGNNTATCGCCTTCT CCCAGTGTAAACTTGTCATTGCTGAGACTGCTTNCCACAAGAGCGATC |
Restriction Sites | ECoRI-NOT |
ACCN | NM_002633 |
Insert Size | 2210 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_002633.2, NP_002624.2 |
RefSeq Size | 2487 bp |
RefSeq ORF | 1689 bp |
Locus ID | 5236 |
UniProt ID | P36871 |
Domains | PGM_PMM, PGM_PMM_I, PGM_PMM_II, PGM_PMM_III |
Protein Pathways | Amino sugar and nucleotide sugar metabolism, Galactose metabolism, Glycolysis / Gluconeogenesis, Metabolic pathways, Pentose phosphate pathway, Starch and sucrose metabolism |
Gene Summary | The protein encoded by this gene is an isozyme of phosphoglucomutase (PGM) and belongs to the phosphohexose mutase family. There are several PGM isozymes, which are encoded by different genes and catalyze the transfer of phosphate between the 1 and 6 positions of glucose. In most cell types, this PGM isozyme is predominant, representing about 90% of total PGM activity. In red cells, PGM2 is a major isozyme. This gene is highly polymorphic. Mutations in this gene cause glycogen storage disease type 14. Alternativley spliced transcript variants encoding different isoforms have been identified in this gene.[provided by RefSeq, Mar 2010] Transcript Variant: This variant (1) is dominant and encodes a protein of 562aa. |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC205771 | PGM1 (Myc-DDK-tagged)-Human phosphoglucomutase 1 (PGM1), transcript variant 1 |
CNY 4,184.00 |
|
RC205771L3 | Lenti ORF clone of Human phosphoglucomutase 1 (PGM1), transcript variant 1, Myc-DDK-tagged |
CNY 6,460.00 |
|
RC205771L4 | Lenti ORF clone of Human phosphoglucomutase 1 (PGM1), transcript variant 1, mGFP tagged |
CNY 6,460.00 |
|
RG205771 | PGM1 (tGFP-tagged) - Human phosphoglucomutase 1 (PGM1), transcript variant 1 |
CNY 5,784.00 |