SMARCE1 (NM_003079) Human Untagged Clone
CAT#: SC118214
SMARCE1 (untagged)-Human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1 (SMARCE1)
CNY 5,488.00
Cited in 1 publication. |
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Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | BAF57; CSS5 |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene sequence for NM_003079 edited
GAATTCGGCACGAGGCTCAGATTCATTCTTAAGGAACTGAGAACTTAATCTTCCAAAATG TCAAAAAGACCATCTTATGCCCCACCTCCCACCCCAGCTCCTGCAACACAAATGCCCAGC ACACCAGGGTTTGTGGGATACAATCCATACAGTCATCTCGCCTACAACAACTACAGGCTG GGAGGGAACCCGGGCACCAACAGCCGGGTCACGGCATCCTCTGGTATCACGATTCCAAAA CCCCCAAAGCCACCAGATAAGCCGCTGATGCCCTACATGAGGTACAGCAGAAAGGTCTGG GACCAAGTAAAGGCTTCCAACCCTGACCTAAAGTTGTGGGAGATTGGCAAGATTATTGGT GGCATGTGGCGAGATCTCACTGATGAAGAAAAACAAGAATATTTAAACGAATACGAAGCA GAAAAGATAGAGTACAATGAATCTATGAAGGCCTATCATAATTCCCCCGCGTACCTTGCT TACATAAATGCAAAAAGTCGTGCAGAAGCTGCTTTAGAGGAAGAAAGTCGACAGAGACAA TCTCGCATGGAGAAAGGAGAACCGTACATGAGCATTCAGCCTGCTGAAGATCCAGATGAT TATGATGATGGCTTTTCAATGAAGCATACAGCCACCGCCCGTTTCCAGAGAAACCACCGC CTCATCAGTGAAATTCTTAGTGAGAGTGTGGTGCCAGACGTTCGGTCAGTTGTCACAACA GCTAGAATGCAGGTCCTCAAACGGCAGGTCCAGTCCTTAATGGTTCATCAGCGAAAACTA GAAGCTGAACTTCTTCAAATAGAGGAACGACACCAGGAGAAGAAGAGGAAATTCCTGGAA AGCACAGATTCATTTAACAATGAACTTAAAAGGTTGTGCGGTCTGAAAGTAGAAGTGGAT ATGGAGAAAATTGCAGCTGAGATTGCACAGGCAGAGGAACAGGCCCGCAAAAGGCAGGAG GAAAGGGAGAAGGAGGCCGCAGAGCAAGCTGAGCGCAGTCAGAGCAGCATCGTTCCTGAG GAAGAACAAGCAGCTAACAAAGGCGAGGAGAAGAAAGACGACGAGAACATTCCGATGGAG ACAGAGGAGACACACCTTGAAGAAACAACAGAGAGCCAACAGAATGGTGAAGAAGGCACG TCTACTCCTGAGGACAAGGAGAGTGGGCAGGAGGGGGTCGACAGTATGGCAGAGGAAGGA ACCAGTGATAGTAACACTGGCTCGGAGAGCAACAGTGCAACAGTGGAGGAGCCACCAACA GATCCCATACCAGAAGATGAGAAAAAAGAATAAGTGTTGCCTTGTTTTGTGTGTTCTAAA TACTTTTTTTAATGAAAAAATGTTTTTTGGTTTTAATGGTGTTACGTGGTTTGTGTATTA ATTTTTTTTCTTGTCCATATCATACCACCAAAGGCTTTTGGACCATTTAGCATCATGAGC CTAATGGCTCAGTCAGTCACCTTTCTTAAGTGTTGTGAAGATGGCTCTTTTCTTTGGATC TTGTTTCTAGCCCTCAACTGCTGAAAGCCTCAGAATTTAGATTAATTGAGAAAACACCCA CCTCTTTTAGAGAATTATCCTTTGATGCTGCAGAATCTACTCTTACAATGCCTTCCTACA GCTCACTGGGGTGCTTACCAAAGCCATAGCTTTAAACCTTCCCAGTCCCCATCAGTAGCT TCCTGAAAGTCTCCTCTCTTGTTTACTTCTGCAAAGGGTAGCTTCTTAAAAACGTGATCA TGTATGAGTATGTATTTGTTCACTTACCCTTTTTTACTTTTAXXXXXXXXXXXXXXXXXX XXXXXXXXXXXXAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTCG AC >OriGene 5' read for NM_003079 unedited
GGTTTGCANATTTTGTATACGATTCCTATAGGCGGCCCGCGAATTCGCACGAGGCTCAGA TTCATTCTTAAGGAACTGAGAACTTAATCTTCCAAAATGTCAAAAAGACCATCTTATGCC CCACCTCCCACCCCAGCTCCTGCAACACAAATGCCCAGCACACCAGGGTTTGTGGGATAC AATCCATACAGTCATCTCGCCTACAACAACTACAGGCTGGGAGGGAACCCGGGCACCAAC AGCCGGGTCACGGCATCCTCTGGTATCACGATTCCAAAACCCCCAAAGCCACCAGATAAG CCGCTGATGCCCTACATGAGGTACAGCAGAAAGGTCTGGGACCAAGTAAAGGCTTCCAAC CCTGACCTAAAGTTGTGGGAGATTGGCAAGATTATTGGTGGCATGTGGCGAGATCTCACT GATGAAGAAAAACAAGAATATTTAAACGAATACGAAGCAGAAAAGATAGAGTACAATGAA TCTATGAAGGCCTATCATAATTCCCCCGCGTACCTTGCTTACATAAATGCAAAAAGTCGT GCAGAAGCTGCTTTAGAGGAAGAAAGTCGACAGAGACAATCTCGCATGGAGAAAGGAGAA CCGTACATGAGCATTCAGCCTGCTGAAGATCCAGATGATTATGATGATGCCTTTTCAATG AAGCATACAGCCACCGCCCGTTTCCAGAAGAACCACCGCCTCATCAGTGAAATTCTTAGT GAGAGTGTGGTGCCAGACGTTCGGTCAGTTGTCACACCAGCTAGAATGCANGTTCTCAAA CGGGCAGTCCCAGTCTTAATGGGTCATCAGCGAAAACCTAAAGCTGAACTTCTCCAATAG AGGCACGACCCCAGNNAGAGAAGAGAAATTCCTCGGAAGCCCANAATCTTTTAACATGAA CTTAAAAGTTGTGCGGTCTGAAAGTACATTGCT >OriGene 3' read for NM_003079 unedited
GCTGGGGTTACTTTGGTCCTTTAANAACATATACCATCAGNAATACCACTATTCAGTAAA AATCTATTCTAGTTTTTGGGAAGCTTATGTGTCACAAACTATCTTGCTTCTAGTCTCCAA GACTTTACTAAGTTTATCAAAAAGGTGGGCTCTGGTCTATGGGATCCTGAGCCTTGAGGT TTGGATGTTCTGATTATTGTAAAACAATTTCAAGTGGATTAACTTAATTCCCAGCTGTTT TTCTTCTGGAATGGGCTAATCAGTTTGAATTATCTAGTCCATTACAAGAATATTAAATTC CTTGAGCTTTGTGATCTATTCTGTCTTTGGAAAACCTGGTAAAAAAGTAATTTTTTTTCT ATACAATAAGGATGGATTTACCAGAACATACCCAGTATTAATCATAACAAAAAGCAGTAT AGATAATGTATATGTGCTGTGTTCACTTTAATAACCACTTCATAAGTAACTACTCTAACT ATGGGGACTATTTCTGGATCAGTAAGATCCAAGTGTAGTTAGTTACACACTACACTCAGC TTAACACAACTCTTGGTATCTGACATTGATTAAAAGTAAAAAAGGGTAAGTGAACAAATA CATACTCATACATGATCACGTTTTTAAGAAGCTACCCTTTGCAGAAGTAAACAAGAGAGG AGACTTTCAGGAAGCTACTGATGGGGACTGGGAAGGGTTAAAGCTATGGCTNTGGTAAGC ACCCCAGTGAGCTGTAGGAAGGCATTGTAAGAGTAGATTCTGCAGCATCAAAGGATAATT CTCCTAAAGAAGTGGGTGTTTTCTCAATTAATCTAAATTCTGAGGCTTTCAGCAGTTTGA GGCTANNAACANGATCCAAAGAAAAAAGCCTCCTCCCACCACTTAAGAAAGTGACTGACT GACCATTAGCTCATGAGCTAAAAGTCCAAAAGCCTTTGTTGTATGAATAGACA |
Restriction Sites | NotI-NotI |
ACCN | NM_003079 |
Insert Size | 2550 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_003079.4, NP_003070.3 |
RefSeq Size | 2425 bp |
RefSeq ORF | 1236 bp |
Locus ID | 6605 |
UniProt ID | Q969G3 |
Domains | HMG |
Protein Families | Transcription Factors |
Gene Summary | The protein encoded by this gene is part of the large ATP-dependent chromatin remodeling complex SWI/SNF, which is required for transcriptional activation of genes normally repressed by chromatin. The encoded protein, either alone or when in the SWI/SNF complex, can bind to 4-way junction DNA, which is thought to mimic the topology of DNA as it enters or exits the nucleosome. The protein contains a DNA-binding HMG domain, but disruption of this domain does not abolish the DNA-binding or nucleosome-displacement activities of the SWI/SNF complex. Unlike most of the SWI/SNF complex proteins, this protein has no yeast counterpart. [provided by RefSeq, Jul 2008] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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SWI/SNF Regulates the Cellular Response to Hypoxia
,Niall S. Kenneth, Sharon Mudie, Patrick van Uden, and Sonia Rocha,
J. Biol. Chem., Feb 2009; 284: 4123 - 4131.
[SMARCE1]
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC209444 | SMARCE1 (Myc-DDK-tagged)-Human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1 (SMARCE1) |
CNY 5,488.00 |
|
RC209444L1 | Lenti ORF clone of Human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1 (SMARCE1), Myc-DDK-tagged |
CNY 7,888.00 |
|
RC209444L2 | Lenti ORF clone of Human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1 (SMARCE1), mGFP tagged |
CNY 7,888.00 |
|
RC209444L3 | Lenti ORF clone of Human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1 (SMARCE1), Myc-DDK-tagged |
CNY 7,888.00 |
|
RC209444L4 | Lenti ORF clone of Human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1 (SMARCE1), mGFP tagged |
CNY 5,890.00 |
|
RG209444 | SMARCE1 (tGFP-tagged) - Human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1 (SMARCE1) |
CNY 7,088.00 |
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