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TCF4 (NM_003199) Human Untagged Clone
CAT＃: SC118127
TCF4 (untagged)-Human transcription factor 4 (TCF4), transcript variant 2
CNY 4,896.00
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | bHLHb19; CDG2T; E2-2; FECD3; ITF-2; ITF2; PTHS; SEF-2; SEF2; SEF2-1; SEF2-1A; SEF2-1B; SEF2-1D; TCF-4 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC118127 sequence for NM_003199 edited (data generated by NextGen Sequencing)
ATGCATCACCAACAGCGAATGGCTGCCTTAGGGACGGACAAAGAGCTGAGTGATTTACTG GATTTCAGTGCGATGTTTTCACCTCCTGTGAGCAGTGGGAAAAATGGACCAACTTCTTTG GCAAGTGGACATTTTACTGGCTCAAATGTAGAAGACAGAAGTAGCTCAGGGTCCTGGGGG AATGGAGGACATCCAAGCCCGTCCAGGAACTATGGAGATGGGACTCCCTATGACCACATG ACCAGCAGGGACCTTGGGTCACATGACAATCTCTCTCCACCTTTTGTCAATTCCAGAATA CAAAGTAAAACAGAAAGGGGCTCATACTCATCTTATGGGAGAGAATCAAACTTACAGGGT TGCCACCAGCAGAGTCTCCTTGGAGGTGACATGGATATGGGCAACCCAGGAACCCTTTCG CCCACCAAACCTGGTTCCCAGTACTATCAGTATTCTAGCAATAATCCCCGAAGGAGGCCT CTTCACAGTAGTGCCATGGAGGTACAGACAAAGAAAGTTCGAAAAGTTCCTCCAGGTTTG CCATCTTCAGTCTATGCTCCATCAGCAAGCACTGCCGACTACAATAGGGACTCGCCAGGC TATCCTTCCTCCAAACCAGCAACCAGCACTTTCCCTAGCTCCTTCTTCATGCAAGATGGC CATCACAGCAGTGACCCTTGGAGCTCCTCCAGTGGGATGAATCAGCCTGGCTATGCAGGA ATGTTGGGCAACTCTTCTCATATTCCACAGTCCAGCAGCTACTGTAGCCTGCATCCACAT GAACGTTTGAGCTATCCATCACACTCCTCAGCAGACATCAATTCCAGTCTTCCTCCGATG TCCACTTTCCATCGTAGTGGTACAAACCATTACAGCACCTCTTCCTGTACGCCTCCTGCC AACGGGACAGACAGTATAATGGCAAATAGAGGAAGCGGGGCAGCCGGCAGCTCCCAGACT GGAGATGCTCTGGGGAAAGCACTTGCTTCGATCTATTCTCCAGATCACACTAACAACAGC TTTTCATCAAACCCTTCAACTCCTGTTGGCTCTCCTCCATCTCTCTCAGCAGGCACAGCT GTTTGGTCTAGAAATGGAGGACAGGCCTCATCGTCTCCTAATTATGAAGGACCCTTACAC TCTTTGCAAAGCCGAATTGAAGATCGTTTAGAAAGACTGGATGATGCTATTCATGTTCTC CGGAACCATGCAGTGGGCCCATCCACAGCTATGCCTGGTGGTCATGGGGACATGCATGGA ATCATTGGACCTTCTCATAATGGAGCCATGGGTGGTCTGGGCTCAGGGTATGGAACCGGC CTTCTTTCAGCCAACAGACATTCACTCATGGTGGGGACCCATCGTGAAGATGGCGTGGCC CTGAGAGGCAGCCATTCTCTTCTGCCAAACCAGGTTCCGGTTCCACAGCTTCCTGTCCAG TCTGCGACTTCCCCTGACCTGAACCCACCCCAGGACCCTTACAGAGGCATGCCACCAGGA CTACAGGGGCAGAGTGTCTCCTCTGGCAGCTCTGAGATCAAATCCGATGACGAGGGTGAT GAGAACCTGCAAGACACGAAATCTTCGGAGGACAAGAAATTAGATGACGACAAGAAGGAT ATCAAATCAATTACTAGCAATAATGACGATGAGGACCTGACACCAGAGCAGAAGGCAGAG CGTGAGAAGGAGCGGAGGATGGCCAACAATGCCCGAGAGCGTCTGCGGGTCCGTGACATC AACGAGGCTTTCAAAGAGCTCGGCCGCATGGTGCAGCTCCACCTCAAGAGTGACAAGCCC CAGACCAAGCTCCTGATCCTCCACCAGGCGGTGGCCGTCATCCTCAGTCTGGAGCAGCAA GTCCGAGAAAGGAATCTGAATCCGAAAGCTGCGTGTCTGAAAAGAAGGGAGGAAGAGAAG GTGTCCTCGGAGCCTCCCCCTCTCTCCTTGGCCGGCCCACACCCTGGAATGGGAGACGCA TCGAATCACATGGGACAGATGTAA Clone variation with respect to NM_003199.2 1929 a=>g >OriGene 5' read for NM_003199 unedited
GTTTTTGTATACGACTCACTATAGGGCGGCNCGCGAATTCGCACGAGGGGCGCGGGAGCG GGCGCAGGAGCAGGCGGCGGCGGTGGCGGCGGCGGTTAGACATGAACGCCGCCTCGGCGC CGGCGGTGCACGGAGAGCCCCTTCTCGCGCGCGGGCGGTTTGTGTGATTTTGCTAAAATG CATCACCAACAGCGAATGGCTGTCTTAGGGACGGACAAAGAGCTGAGTGATTTACTGGAT TTCAGTGCGATGTTTTCACCTCCTGTGAGCAGTGGGAAAAATGGACCAACTTCTTTGGCA AGTGGACATTTTACTGGCTCAAATGTAGAAGACAGAAGTAGCTCAGGGTCCTGGGGGAAT GGAGGACATCCAAGCCCGTCCAGGAACTATGGAGATGGGACTCCCTATGACCACATGACC AGCAGGGACCTTGGGTCACATGACAATCTCTCTCCACCTTTTGTCAATTCCAGAATACAA AGTAAAACAGAAAGGGGCTCATACTCATCTTATGGGAGAGAATCAAACTTACAGGGTTGC CACCAGCAGAGTCTCCTTGGAGGTGACATGGATATGGGCAACCCAGGAACCCTTTCGCCC ACCAAACCTGGTTCCCAGTACTATCAGTATTCTAGCAATAATCCCCGAAGGAGGCCTCTT CACAGTAGTGCCATGGAGGTACAGACAAAGAAAGTTCGAAAAGTTCCTCCAGGTTTGCCA TCTTCAGTCTATGCTCCATCAGCAAGCACTGCCGACTACAATAGGGACTCGCCAGGCTAT TCTTCCTCCAAACAGCAACCAGCACTTTCCCTAGCTCTTTTTTATGCAAGATGGCATCAC AGCAGTGACCCTTGGAGCTCCTTCATGGGATGAATCCCCCTGCCTTTCAGGGATGTTGGG CAACTCTTTTCATATTCACAGTCCACACACTCTTGACTCTGATTCCACTGAACCTT >OriGene 3' read for NM_003199 unedited
CGGCACGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTGTTTTTCTGTTTTTTGATAA TTGGGAATGCTGAAACCTCTTGCGTCTGCGATTCATAACTACTCAGACTTGTCTTATATT ACAAAAATGGGGGTTAAGGAGAAGTGTTTATGTGGGTTTAAGATAATACAGCTGTTAAGG AAGTGGTCTCTTGTTTTAATGAAGCAATGTGGCAACTTGGACCCTTTTACATCTGTCCCA TGTGATTCGATGCGTCTCCCATTCCAGGGTGTGGGCCGGCCAAGGAGAGAGGGGGAGGCT CCGAGGACACCTTCTCTTCCTCCCTTCTTTTCAGACACGCAGCTTTCGGATTCAGATTCC TTTCTCGGACTTGCTGCTCCAGACTGAGGATGACGGCCACCGCCTGGTGGAGGATCAGGA GCTTGGTCTGGGGCTTGTCACTCTTGAGGTGGAGCTGCACCATGCGGCCGAGCTCTTTGA AAGCCTCGTTGATGTCACGGACCCGCAGACGCTCTCGGGCATTGTTGGCCATCCTCCGCT CCTTCTCACGCTCTGCCTTCTGCTCTGGTGTCAGGTCCTCATCGTCATTATTGCTAGTAA TTGATTTGATATCCTTCTTGTCGTCATCTAATTTCTTGTCCTCCGAAGATTTCGTGTCTT GCAAGTTCTCATCACCCTCGTCATCGGATTTGATCTCAGAGCTGCCAGAGGAGACACTCT GCCCCTGTAGTCCTGGTGGCATGCCTCTGNTAGGGTCCCTGGGGTGGGTTCAGGTCAGGG GAAGTCGCAAACTGGACAGNAAGCTGTGGAACCCGNACCTGNNNTTTGCAGAAAGAATGG CTGNCTCTCAGGNCCACGCATCTCACGATGGNTCCCACCATGANTGAATGTCTGTTGGCT GAAAAAAGGCCGTTCCATACCCTGAGCCCAGACANCCATGGCTCTTATGAGAAGTCCATG ATTCATGCTGTCCCTGAA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_003199 |
| Insert Size | 2340 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003199.1, NP_003190.1 |
| RefSeq Size | 2500 bp |
| RefSeq ORF | 2004 bp |
| Locus ID | 6925 |
| UniProt ID | P15884 |
| Domains | HLH |
| Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Transcription Factors |
| Gene Summary | This gene encodes transcription factor 4, a basic helix-loop-helix transcription factor. The encoded protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif first identified in immunoglobulin enhancers. This gene is broadly expressed, and may play an important role in nervous system development. Defects in this gene are a cause of Pitt-Hopkins syndrome. In addition, an intronic CTG repeat normally numbering 10-37 repeat units can expand to >50 repeat units and cause Fuchs endothelial corneal dystrophy. Multiple alternatively spliced transcript variants that encode different proteins have been described. [provided by RefSeq, Jul 2016] Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence and uses an alternate in-frame splice site at the 3' end of an exon compared to variant 3. The resulting isoform (b, also known as TCF4-B-) is shorter at the N-terminus and lacks an alternate internal segment compared to isoform c. Sequence Note: This gene is distinct from TCF7L2 (alias TCF-4). |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217718 | TCF4 (Myc-DDK-tagged)-Human transcription factor 4 (TCF4), transcript variant 2 |
CNY 4,888.00 |
|
| RC217718L1 | Lenti ORF clone of Human transcription factor 4 (TCF4), transcript variant 2, Myc-DDK-tagged |
CNY 7,288.00 |
|
| RC217718L2 | Lenti ORF clone of Human transcription factor 4 (TCF4), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RC217718L3 | Lenti ORF clone of Human transcription factor 4 (TCF4), transcript variant 2, Myc-DDK-tagged |
CNY 7,288.00 |
|
| RC217718L4 | Lenti ORF clone of Human transcription factor 4 (TCF4), transcript variant 2, mGFP tagged |
CNY 7,288.00 |
|
| RG217718 | TCF4 (tGFP-tagged) - Human transcription factor 4 (TCF4), transcript variant 2 |
CNY 6,488.00 |
