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Topoisomerase I (TOP1) (NM_003286) Human Untagged Clone
CAT＃: SC118105
TOP1 (untagged)-Human topoisomerase (DNA) I (TOP1)
CNY 7,220.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | TOPI |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_003286 edited
ATGAGTGGGGACCACCTCCACAACGATTCCCAGATCGAAGCGGATTTCCGATTGAATGAT TCTCATAAACACAAAGATAAACACAAAGATCGAGAACACCGGCACAAAGAACACAAGAAG GAGAAGGACCGGGAAAAGTCCAAGCATAGCAACAGTGAACATAAAGATTCTGAAAAGAAA CACAAAGAGAAGGAGAAGACCAAACACAAAGATGGAAGCTCAGAAAAGCATAAAGACAAA CATAAAGACAGAGACAAGGAAAAACGAAAAGAGGAAAAGGTTCGAGCCTCTGGGGATGCA AAAATAAAGAAGGAGAAGGAAAATGGCTTCTCTAGTCCACCACAAATTAAAGATGAACCT GAAGATGATGGCTATTTTGTTCCTCCTAAAGAGGATATAAAGCCATTAAAGAGACCTCGA GATGAGGATGATGCTGATTATAAACCTAAGAAAATTAAAACAGAAGATACCAAGAAGGAG AAGAAAAGAAAACTAGAAGAAGAAGAGGATGGTAAATTGAAAAAACCCAAGAATAAAGAT AAAGATAAAAAAGTTCCTGAGCCAGATAACAAGAAAAAGAAGCCGAAGAAAGAAGAGGAA CAGAAGTGGAAATGGTGGGAAGAAGAGCGCTATCCTGAAGGCATCAAGTGGAAATTCCTA GAACATAAAGGTCCAGTATTTGCCCCACCATATGAGCCTCTTCCAGAGAATGTCAAGTTT TATTATGATGGTAAAGTCATGAAGCTGAGCCCCAAAGCAGAGGAAGTAGCTAC:GTTC:T TTGCAAAAATGCTCGACCATGAATATACTACCAAGGAAATATTTAGGAAAAATTTCTTTA AAGACTGGAGAAAGGAAATGACTAATGAAGAGAAGAATATTATCACCAACCTAAGCAAAT GTGATTTTACCCAGATGAGCCAGTATTTCAAAGCCCAGACGGAAGCTCGGAAACAGATGA GCAAGGAAGAGAAACTGAAAATCAAAGAGGAGAATGAAAAATTACTGAAAGAATATGGAT TCTGTATTATGGATAACCACAAAGAGAGGATTGCTAACTTCAAGATAGAGCCTCCTGGAC TTTTCCGTGGCCGCGGCAACCACCCCAAGATGGGCATGCTGAAGAGACGAATCATGCCCG AGGATATAATCATCAACTGTAGCAAAGATGCCAAGGTTCCTTCTCC:TCCTCCAGGACAT AAGTGGAAAGAAGTCCGGCATGATAACAAGGTTACTTGGCTGGTTTCCTGGACAGAGAAC ATCCAAGGTTCCATTAAATACATCATGCTTAACCCTAGTTCACGAATCAAGGGTGAGAAG GACTGGCAGAAATACGAGACTGCTCGGCGGCTGAAAAAATGTGTGGACAAGATCCGGAAC CAGTATCGAGAAGACTGGAAGTCCAAAGAGATGAAAGTCCGGCAGAGAGCTGTAGCCCTG TACTTCATCGACAAGCTTGCTCTGAGAGCAGGCAATGAAAAGGAGGAAGGAGAAACAGCG GACACTGTGGGCTGCTGCTCACTTCGTGTGGAGCACATCAATCTACACCCAGAGTTGGAT GGTCAGGAATATGTGGTAGAGTTTGACTTCCTCGGGAAGGACTCCATCAGATACTATAAC AAGGTCCCTGTTGAGAAACGAGTTTTTAAGAACCTACAACTATTTATGGAGAACAAGCAG CCCGAGGATGATCTTTTTGATAGACTCAATACTGGTATTCTGAATAAGCATCTTCAGGAT CTCATGGAGGGCTTGACAGCCAAGGTATTCCGTACATACAATGCCTCCATCACGCTACAG CAGCAGCTAAAAGAACTGACAGCCCCGGATGAGAACATCCCAGCGAAGATCCTTTCTTAT AACCGTGCCAATCGAGCTGTTGCAATTCTTTGTAACCATCAGAGGGCACCACCAAAAACT TTTGAGAAGTCTATGATGAACTTGCAAACTAAGATTGATGCCAAGAAGGAACAGCTAGCA GATGCCCGGAGAGACCTGAAAAGTGCTAAGGCTGATGCCAAGGTCATGAAGGATGCAAAG ACGAAGAAGGTAGTAGAGTCAAAGAAGAAGGCTGTTCAGAGACTGGAGGAACAGTTGATG AAGCTGGAAGTTCAAGCCACAGACCGAGAGGAAAATAAACAGATTGCCCTGGGAACCTCC AAACTCAATTATCTGGACCCTAGGATCACAGTGGCTTGGTGCAAGAAGTGGGGTGTCCCA ATTGAGAAGATTTACAACAAAACCCAGCGGGAGAAGTTTGCCTGGGCCATTGACATGGCT GATGAAGACTATGAGTTTTAG >OriGene 5' read for NM_003286 unedited
TTTTGTAATACGACTCCACTATAGGGCCGCGCCGCGCAATTCGGCACGAGGGTTACACAA CTGCTGGGGTCTGTTCTCGCCGCCCGCCCGGCAGTCAGGCAGCGTCGCCGCCGTGGTAGC AGCCTCAGCCGTTTCTGGAGTCTCGGGCCCACAGTCACCGCCGCTTACCTGCGCCTCCTC GAGCCTCCGGAGTCCCCGTCCGCCCGCACAGGCCGGTTCGCCGTCTGCGTCTCCCCCACG CCGCCTCGCCTGCCGCCGCGCTCGTCCCTCCGGGCCGACATGAGTGGGGACCACCTCCAC AACGATTCCCAGATCGAAGCGGATTTCCGATTGAATGATTCTCATAAACACAAAGATAAA CACAAAGATCGAGAACACCGGCACAAAGAACACAAGAAGGAGAAGGACCGGGAAAAGTCC AAGCATAGCAACAGTGAACATAAAGATTCTGAAAAGAAACACAAAGAGAAGGAGAAGACC AAACACAAAGATGGAAGCTCAGAAAAGCATAAAGACAAACATAAAGACAGAGACAAGGAA AAACGAAAAGAGGAAAAGGTTCGAGCCTCTGGGGATGCAAAATAAAGAAGGAGAAGGAAA ATGGCTTCTCTAGTCCACCACATATTAAAGATGAACCTGAAGATGATGGCTATTTTGTTC CTCCTAAAGAGGATTATAAGCCATTAAAGAGACCTCGAGATGAGGATGATGCTGATTATT ACCCTAGGAAATTAAACCAGAGGATCCCAGAAAGAGATGAAAGAAACTTGAAGAGAAGAG GGTGGTAAATTGAAAAACCCAAAATTAAGTACAAGAAAAAAAGTTCCTGGGCCCTCATAC ATGAACAGCACCCCTAGCAAGAAGAGAACCTAACGGAAATGGTGGAAGAACAGCCCTTCC TGAAGCCTT >OriGene 3' read for NM_003286 unedited
CTATGCTACCGCGTGCCGCATNCTAAAATCGAGTTTTTTTTTTTTTTTTTTCCTTAAGCT GTAGGAATTTATTTTAAATAACCTACAGTTGATTAAAAGGGAATTCATGATAAAAATACA AGATACTTGAGGAAAGATGTGGGAAATGGACTCTGCACACACACTAAACTAACAATGCCT CTAAAACTAATGATTATAGCAAAAAATGTCTTCACATTAAAATTCTGCTTTTTATGTTTT TTTCCATTTTTTACACAATTACAAAAGAAAAAATAAAAGCCCTAAAATCTTGATTATTTT TCCTTTTTTTGGACCAAATACTCATTTTCCTCTAAGTTTATTGACCTGTGAAACTTTTTA TACAATAAAATCTTTCAAGTGAAAGATTAGGGTTAAAAAGAAAAAGATGGATATCTTAAA GGGTACAGCGAATGCTCAGAACAAAGGATGATGGGCAAAATGGTTTCAGCCACTGATTAT TCCATTATCCTTAGATTCACTCGCCCTTGATCCCTCCCCAACCCCAATTTACACGATCTT TAAGATCAAGAAAAAGGTTTAAATATTTTAAAATAATCAAAAAGAAATAAAAATTCACAT TTAAAAAGGAACACTGAAGCCAGGAAACATTTCGCCATCATGCTTTCCCTGTGAACAGGT GTTTGACCCAAAACACTGCCAAAGGCACGACCGCTTCAAGCTTAATGAAAATTGATCCCC CCGACAATAGAGAGTGACGACTCTAACAGGCGCGCCGGGATTTTTGCCCTACTTAACCTT AACACCTGAAAGGGAGAGAGCAAGGGAGCCTAGAATTTATTCCTATCCAATATAGAGCTA TTGGGAACCCTACTGTAAAAAGCACGATATAACCGACCTTCATCACGCCCTAAGAGACCC AACTTTCACGAAACGCGAACTGCCTGGAAAACCTGAAACATCCAATCCTGCCTCTGGAAC AAAACAGATTCAACACAT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_003286 |
| Insert Size | 2298 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003286.2, NP_003277.1 |
| RefSeq Size | 3734 bp |
| RefSeq ORF | 2298 bp |
| Locus ID | 7150 |
| UniProt ID | P11387 |
| Domains | TOPEUc |
| Protein Families | Druggable Genome |
| Gene Summary | This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC215658 | TOP1 (Myc-DDK-tagged)-Human topoisomerase (DNA) I (TOP1) |
CNY 3,990.00 |
|
| RC215658L1 | Lenti-ORF clone of TOP1 (Myc-DDK-tagged)-Human topoisomerase (DNA) I (TOP1) |
CNY 10,800.00 |
|
| RC215658L2 | Lenti-ORF clone of TOP1 (mGFP-tagged)-Human topoisomerase (DNA) I (TOP1) |
CNY 5,890.00 |
|
| RC215658L3 | Lenti-ORF clone of TOP1 (Myc-DDK-tagged)-Human topoisomerase (DNA) I (TOP1) |
CNY 5,890.00 |
|
| RC215658L4 | Lenti-ORF clone of TOP1 (mGFP-tagged)-Human topoisomerase (DNA) I (TOP1) |
CNY 5,890.00 |
|
| RG215658 | TOP1 (tGFP-tagged) - Human topoisomerase (DNA) I (TOP1) |
CNY 10,000.00 |
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