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Topoisomerase I (TOP1) (NM_003286) Human Tagged ORF Clone
CAT＃: RC215658
- TrueORF®
TOP1 (Myc-DDK-tagged)-Human topoisomerase (DNA) I (TOP1)
ORF Plasmid: tGFP
"NM_003286" in other vectors (6)
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CNY 3,990.00
| Cited in 1 publication. |
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | TOPI |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC215658 representing NM_003286
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGGGGACCACCTCCACAACGATTCCCAGATCGAAGCGGATTTCCGATTGAATGATTCTCATAAAC ACAAAGATAAACACAAAGATCGAGAACACCGGCACAAAGAACACAAGAAGGAGAAGGACCGGGAAAAGTC CAAGCATAGCAACAGTGAACATAAAGATTCTGAAAAGAAACACAAAGAGAAGGAGAAGACCAAACACAAA GATGGAAGCTCAGAAAAGCATAAAGACAAACATAAAGACAGAGACAAGGAAAAACGAAAAGAGGAAAAGG TTCGAGCCTCTGGGGATGCAAAAATAAAGAAGGAGAAGGAAAATGGCTTCTCTAGTCCACCACAAATTAA AGATGAACCTGAAGATGATGGCTATTTTGTTCCTCCTAAAGAGGATATAAAGCCATTAAAGAGACCTCGA GATGAGGATGATGCTGATTATAAACCTAAGAAAATTAAAACAGAAGATACCAAGAAGGAGAAGAAAAGAA AACTAGAAGAAGAAGAGGATGGTAAATTGAAAAAACCCAAGAATAAAGATAAAGATAAAAAAGTTCCTGA GCCAGATAACAAGAAAAAGAAGCCGAAGAAAGAAGAGGAACAGAAGTGGAAATGGTGGGAAGAAGAGCGC TATCCTGAAGGCATCAAGTGGAAATTCCTAGAACATAAAGGTCCAGTATTTGCCCCACCATATGAGCCTC TTCCAGAGAATGTCAAGTTTTATTATGATGGTAAAGTCATGAAGCTGAGCCCCAAAGCAGAGGAAGTAGC TACGTTCTTTGCAAAAATGCTCGACCATGAATATACTACCAAGGAAATATTTAGGAAAAATTTCTTTAAA GACTGGAGAAAGGAAATGACTAATGAAGAGAAGAATATTATCACCAACCTAAGCAAATGTGATTTTACCC AGATGAGCCAGTATTTCAAAGCCCAGACGGAAGCTCGGAAACAGATGAGCAAGGAAGAGAAACTGAAAAT CAAAGAGGAGAATGAAAAATTACTGAAAGAATATGGATTCTGTATTATGGATAACCACAAAGAGAGGATT GCTAACTTCAAGATAGAGCCTCCTGGACTTTTCCGTGGCCGCGGCAACCACCCCAAGATGGGCATGCTGA AGAGACGAATCATGCCCGAGGATATAATCATCAACTGTAGCAAAGATGCCAAGGTTCCTTCTCCTCCTCC AGGACATAAGTGGAAAGAAGTCCGGCATGATAACAAGGTTACTTGGCTGGTTTCCTGGACAGAGAACATC CAAGGTTCCATTAAATACATCATGCTTAACCCTAGTTCACGAATCAAGGGTGAGAAGGACTGGCAGAAAT ACGAGACTGCTCGGCGGCTGAAAAAATGTGTGGACAAGATCCGGAACCAGTATCGAGAAGACTGGAAGTC CAAAGAGATGAAAGTCCGGCAGAGAGCTGTAGCCCTGTACTTCATCGACAAGCTTGCTCTGAGAGCAGGC AATGAAAAGGAGGAAGGAGAAACAGCGGACACTGTGGGCTGCTGCTCACTTCGTGTGGAGCACATCAATC TACACCCAGAGTTGGATGGTCAGGAATATGTGGTAGAGTTTGACTTCCTCGGGAAGGACTCCATCAGATA CTATAACAAGGTCCCTGTTGAGAAACGAGTTTTTAAGAACCTACAACTATTTATGGAGAACAAGCAGCCC GAGGATGATCTTTTTGATAGACTCAATACTGGTATTCTGAATAAGCATCTTCAGGATCTCATGGAGGGCT TGACAGCCAAGGTATTCCGTACATACAATGCCTCCATCACGCTACAGCAGCAGCTAAAAGAACTGACAGC CCCGGATGAGAACATCCCAGCGAAGATCCTTTCTTATAACCGTGCCAATCGAGCTGTTGCAATTCTTTGT AACCATCAGAGGGCACCACCAAAAACTTTTGAGAAGTCTATGATGAACTTGCAAACTAAGATTGATGCCA AGAAGGAACAGCTAGCAGATGCCCGGAGAGACCTGAAAAGTGCTAAGGCTGATGCCAAGGTCATGAAGGA TGCAAAGACGAAGAAGGTAGTAGAGTCAAAGAAGAAGGCTGTTCAGAGACTGGAGGAACAGTTGATGAAG CTGGAAGTTCAAGCCACAGACCGAGAGGAAAATAAACAGATTGCCCTGGGAACCTCCAAACTCAATTATC TGGACCCTAGGATCACAGTGGCTTGGTGCAAGAAGTGGGGTGTCCCAATTGAGAAGATTTACAACAAAAC CCAGCGGGAGAAGTTTGCCTGGGCCATTGACATGGCTGATGAAGACTATGAGTTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC215658 representing NM_003286
Red=Cloning site Green=Tags(s) MSGDHLHNDSQIEADFRLNDSHKHKDKHKDREHRHKEHKKEKDREKSKHSNSEHKDSEKKHKEKEKTKHK DGSSEKHKDKHKDRDKEKRKEEKVRASGDAKIKKEKENGFSSPPQIKDEPEDDGYFVPPKEDIKPLKRPR DEDDADYKPKKIKTEDTKKEKKRKLEEEEDGKLKKPKNKDKDKKVPEPDNKKKKPKKEEEQKWKWWEEER YPEGIKWKFLEHKGPVFAPPYEPLPENVKFYYDGKVMKLSPKAEEVATFFAKMLDHEYTTKEIFRKNFFK DWRKEMTNEEKNIITNLSKCDFTQMSQYFKAQTEARKQMSKEEKLKIKEENEKLLKEYGFCIMDNHKERI ANFKIEPPGLFRGRGNHPKMGMLKRRIMPEDIIINCSKDAKVPSPPPGHKWKEVRHDNKVTWLVSWTENI QGSIKYIMLNPSSRIKGEKDWQKYETARRLKKCVDKIRNQYREDWKSKEMKVRQRAVALYFIDKLALRAG NEKEEGETADTVGCCSLRVEHINLHPELDGQEYVVEFDFLGKDSIRYYNKVPVEKRVFKNLQLFMENKQP EDDLFDRLNTGILNKHLQDLMEGLTAKVFRTYNASITLQQQLKELTAPDENIPAKILSYNRANRAVAILC NHQRAPPKTFEKSMMNLQTKIDAKKEQLADARRDLKSAKADAKVMKDAKTKKVVESKKKAVQRLEEQLMK LEVQATDREENKQIALGTSKLNYLDPRITVAWCKKWGVPIEKIYNKTQREKFAWAIDMADEDYEF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003286 |
| ORF Size | 2295 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003286.4 |
| RefSeq Size | 3734 bp |
| RefSeq ORF | 2298 bp |
| Locus ID | 7150 |
| UniProt ID | P11387 |
| Domains | TOPEUc |
| Protein Families | Druggable Genome |
| MW | 90.5 kDa |
| Gene Summary | This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22. [provided by RefSeq, Jul 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
FL118, acting as a ‘molecular glue degrader’, binds to dephosphorylates and degrades the oncoprotein DDX5 (p68) to control c‐Myc, survivin and mutant Kras against colorectal and pancreatic cancer with high efficacy, et al. FL118, acting as a ‘molecular glue degrader’, binds to dephosphorylates and degrades the oncoprotein DDX5 (p68) to control c‐Myc, survivin and mutant Kras against colorectal and pancreatic cancer with high efficacy
,null,
Clinical and Translational Medicine
,PubMed ID 35604033
[Topoisomerase I]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC215658L1 | Lenti-ORF clone of TOP1 (Myc-DDK-tagged)-Human topoisomerase (DNA) I (TOP1) |
CNY 10,800.00 |
|
| RC215658L2 | Lenti-ORF clone of TOP1 (mGFP-tagged)-Human topoisomerase (DNA) I (TOP1) |
CNY 5,890.00 |
|
| RC215658L3 | Lenti-ORF clone of TOP1 (Myc-DDK-tagged)-Human topoisomerase (DNA) I (TOP1) |
CNY 5,890.00 |
|
| RC215658L4 | Lenti-ORF clone of TOP1 (mGFP-tagged)-Human topoisomerase (DNA) I (TOP1) |
CNY 5,890.00 |
|
| RG215658 | TOP1 (tGFP-tagged) - Human topoisomerase (DNA) I (TOP1) |
CNY 10,000.00 |
|
| SC118105 | TOP1 (untagged)-Human topoisomerase (DNA) I (TOP1) |
CNY 7,220.00 |
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